Biological Waste Disposal Table

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1 Biological Disposal Table Type of Animal bedding, cage, water bottle from infected animals Animal carcasses and tissue (including transgenic animal tissue) Human gans, tissue, body parts (pathological ) Liquid biohazardous including all r/snas* material Place cage, water bottle and bedding in clear autoclave bag as directed by RAR. (If animals housed outside of RAR, return bag to RAR) Place in digestible bag in RAR designated red Digestible bag in U Market #GC20009 (small) #GC20010 (large) Program liquid handling. Autoclaved by RAR staff. Disinfect with 1 part bleach to 9 parts liquid, approved disinfectant per an IBC protocol, and sewer. Can also autoclave and sewer. (Do not sewer agar; handle Place autoclaved bedding in regular trash. See RAR Animal Care Services f the location of animal coolers. Program Not applicable Program Not applicable

2 Type of Contaminated nonsharps (including r/snas*) Glass (not hazardous material) Sharps, whether not contaminated Liquid mixed biohazardous and (including r/snas*) Clear autoclave bag etc Red biohazardous bag such as U Market # etc. Dedicated box closed and taped shut (use separate boxes f broken & non broken glass and label as such) Sharps etc Place in liquid handling, label as. as solid.) Autoclave at 121ºC f 60 min. See Autoclaving Biological Disinfect with 1 part bleach to 9 parts liquid IBC approved disinfectant. autoclaving place in regular NOTE: Red bags cannot be placed in hallways other public areas. NOTE: Sharps s can t be placed in hallways other public areas. disinfecting place in liquid. Call f a request if biohazardous material is not nmally picked up in your area. call f a request if biohazardous material isn t nmally picked up in your area. Manual

3 Type of Solid mixed biohazardous and (including r/snas*) animal Liquid prions Place solid in clear autoclave bag, attach autoclave tape, label as. See Manual liquid handling. NOTE: Do not use bleach with iodine. Autoclave at 121 C f 60 min. See Autoclaving Biological See Manual Dispose in VDL Tissue Digester autoclaving place in solid. See Manual Not applicable Manual UHS Not applicable autoclave at 134 C f 1 hour and sewer treat with 40% bleach 2N NaOH f 1 hour and neutralize with acid until ph is between 5 and 8 and sewer hold f chemical disposal.

4 Solid prions Sharps prions Type of Animal tissue and carcasses prions Biological toxins Place non tissue low level solid in yellow bag in yellow Sharps etc. Place in digestible bag in RAR designated red Digestible bag in U Market #GC20009 (small) #GC20010 (large) f treatment Place sharps in yellow bag Liquid : Treat with 10% (1:9 v/v) bleach f at least 30 minutes other proven effective inactivating agent. Solid containing low molecular weight toxin: Place in a yellow See RAR Animal Care Services f the location of animal coolers. If the toxin is in a hazardous chemical mixture, put in hazardous chemical after treatment with inactivating agent no longer a toxin hazard f yellow bag & yellow barrel delivery & 6481 f Hazardous Chemical Management Guidebook 6481 f yellow bag & yellow barrel delivery &

5 bag f incineration. Solid containing proteinaceous toxin: Autoclave at 121ºC f 60 min place in a red biohazard bag. Note: r/snas refers to recombinant/synthetic Nucleic Acids. Place autoclaved bag in regular Place red bag next to regular