Antibodies & Imaging Systems

Transcription

1 Antibodies & Imaging Systems Cancer Neuroscience Molecular Biology Cell Biology Proteomics Stem Cell Biochemistry

2 VWR Gel Imagers Capture, preview, and save gel images with user-friendly features that enable fast, easy-to-use gel documentation. The simple design is excellent for multiple use laboratories. VWR Gel Imagers are available in two different styles with two different camer and door designs. Gel Imagers feature a 0.3MP camera with a wide front door; Gel Imager Plus models are available with a 1.3MP camera and both a wide front door and side access doors. An external computer is not required, although network connectivity is possible for transferring images directly to a computer. Live See a live preview of the gel Snap Capture images and Save the snapped image to TIFF, GIF, and JPEG file formats Exp Warn Set the exposure warning Print Connect to an optional printer Time Stamp Set the image date and time Preferences Set user preferences Integration Increase or decrease exposure time Choice of monochrome CCD Camera megapixels (Gel Imager) or 1.3 megapixels (Gel Imager Plus) Zoom Lens (manual) located below the camera is easily accessible for adjustment of aperture, zoom and focus Ethidium Bromide Filter in the slide-out filter tray allows easy exchange of the filter Large 8" Touch Screen displays live preview of images; the screen tilts for optimum viewing angle Save images to the USB Stick (included) or connect to a network to transfer images to another computer Epi White Light for focusing and illumination of samples Unique Viewer Window allows visibility of gels without opening the door Wide Front Door with safety switch turns UV off when the door is open Side Access Doors (imagers with 1.3 camera only) allow gels to be moved or cut while viewing the gel fluorescence through the window Compact Design for minimal laboratory bench space Choice of UV Transilluminator Dimensions External with Camera: 78H x 36.1W x 33.8Dcm (30 3 4" x " x ") Internal Darkroom: 32.5H x 30.5W x 29.7D cm (11 3 4" x 12" x ") Ask a VWR Representative about accessories including: Thermal Printer and Paper Converter Plates: Convert UV to white light for Coomassie Blue and Silver Stains Gel Cutter and Gel Tools Analysis Software Specifications Darkroom: Wide front door, Viewer window, UV safety switch Epi white light, Side access doors (select models) USB Drive: USB 2.0 (1 GB) LCD Screen: 8" color, touch screen with stylus pen Filter: Ethidium bromide Software: Integrated controls Transilluminator: Filter size: 20 x 20cm (7 7 8" x 7 7 8") or 21 x 26cm (8 1 4" x ") Wavelength: 302nm or 302/365nm UV Power: 115V/60Hz or 230V/50Hz Network: FTP connection 0.3 Megapixel Camera (for Gel Imager) CCD Imager: Monochrome 8-bit scientific grade CCD Resolution: 0.3 megapixels Pixel Resolution: 640 x 480 Grayscale: Lens: mm zoom 1.3 Megapixel Camera (for Gel Imager Plus) CCD Imager: Monochrome 12-bit scientific grade CCD; 16-bit file bit depth Resolution: 1.3 megapixels Pixel Resolution: 1280 x 1024 Grayscale: 0-65,535 Lens: 8-48 mm zoom Ordering Information Transilluminator Filter UV Wavelength Electrical Cat. No. cm (in.) nm Gel Imager, 0.3 MP Camera 20 x V x V x / V x / V x V x V x / V x / V Gel Imager Plus, 1.3 MP Camera, Side Access Doors 20 x V x V x / V x / V x V x V x / V x / V

3 VWR Power Supply For a wide range of applications including DNA, RNA, and protein separations, isoelectric focusing, and blotting. Each unit features operating modes for constant voltage or constant current with automatic crossover. Modular Mini PAGE System The VWR Mini Vertical PAGE System is designed to meet your PAGE, electroblotting and 2D electrophoresis needs. The system is modular purchase just the components you need. A common buffer tank accepts three inserts: the PAGE insert accommodates up to 4 mini gels. The electroblotting insert comes complete with three blotting cassettes for quick transfers. Up to 10 tube gels can be run simultaneously in the 2D gel insert. Customizable accessories are available. The VWR Power Supplies feature constant voltage or current with automatic crossover. Model 202 has a maximum of 250V and 3,000mA, while Model 302 offers a maximum of 300V and 500mA. For PAGE, Electroblotting and 2D Electrophoresis Leakproof, modular design Accepts most 10x10 precast gels Description Cat. No. Mini Vertical PAGE System, 250mL Mini PAGE Electroblotting System Western Blotting System Users can program a preset time or set the timer to run continuously. Units also feature integral folding feet to allow for easy viewing of the LCD screen. Four sets of color coded output terminals allow multiple gels to be run simultaneously. Each unit measures 19W x 25D x 8H cm (7 1 2" x 9 7 8" x ") and is stackable to save space. Compact Design with Small Footprint Designed with Safety in Mind Description Volt Amp Cat. No. Model V 3A Model V 500mA VWR DNA MW Markers These new VWR DNA molecular weight markers are available in 1Kb and 100 bp sizes in ready-to-use format. They are ideal for sizing and quantification of wide range double stranded DNA fragments and PCR products. The 100bp DNA ladder contains 11 discrete DNA fragments ranging from 100bp to 2,000bp. This marker is ideal for the size determination of PCR products. The marker comes in a ready-to-use mixture at a concentration of 0.1µg/µl. The 1Kb Ladder contains 13 discrete DNA fragments ranging in size from 300 to 10,000bp. This marker is ideal for size determination of digested DNA. The marker is ready-to-use mixture at a concentration of 0.1µg/µl. Wide range markers 1Kb and 100bp Ready-to-use format 0.1µg/µl contains glycerol and bromophenol blue Description Size Cat. No. VWR DNA Molecular Weight Markers 1 Kb (500 µl) VWR DNA Molecular Weight Markers 100 Kb (500 µl)

4 BDH Molecular Biology Reagents that make it easier for your lab Description CAS No. Size Packaging Cat. No. ACES g Poly Bottle BDH G ACES g Poly Bottle BDH G Agarose 3:1, High Resolution Blend 100 g Poly Bottle BDH G Agarose 3:1, High Resolution Blend 250 g Poly Bottle BDH G Agarose SFR 100 g Poly Bottle BDH G Agarose SFR 250 g Poly Bottle BDH G Ammonium Sulfate kg Poly Bottle BDH4108-1KG L-Asparagine, Anhydrous g Poly Bottle BDH GP L-Asparagine, Anhydrous kg Poly Bottle BDH4506-1KGP L-Asparagine, Monohydrate g Poly Bottle BDH GP L-Asparagine, Monohydrate kg Poly Bottle BDH4508-1KGP BIS-TRIS g Poly Bottle BDH GP BIS-TRIS kg Poly Bottle BDH4512-1KGP Boric Acid g Poly Bottle BDH G Boric Acid kg Poly Bottle BDH4118-1KG Calcium Chloride, Anhydrous g Poly Bottle BDH GP Calcium Chloride, Anhydrous kg Poly Bottle BDH4524-1KGP Cesium Chloride g Poly Bottle BDH G Cesium Chloride g Poly Bottle BDH G Cesium Chloride kg Poly Bottle BDH4124-1KG Cesium Chloride, DNase- and RNase-free g Poly Bottle BDH G Cesium Chloride, DNase- and RNase-free g Poly Bottle BDH G Cesium Sulfate g Poly Bottle BDH G Cesium Sulfate g Poly Bottle BDH G Citric Acid Trisodium Salt, Dihydrate g Poly Bottle BDH G Citric Acid Trisodium Salt, Dihydrate kg Poly Bottle BDH4136-1KG L-Cystine Dihydrochloride g Poly Bottle BDH GP L-Cystine Dihydrochloride kg Poly Bottle BDH4510-1KGP Dextrose kg Poly Bottle BDH4526-1KGP EDTA Disodium Salt g Poly Bottle BDH GP EDTA Disodium Salt kg Poly Bottle BDH4528-1KGP L-Glutamine g Poly Bottle BDH GP L-Glutamine kg Poly Bottle BDH4514-1KGP Glycerol ml Poly Bottle BDH ML Glycerol L Poly Bottle BDH4154-1L Glycine g Poly Bottle BDH G Glycine kg Poly Bottle BDH4156-1KG Guanidine Hydrochloride g Poly Bottle BDH GP Guanidine Hydrochloride kg Poly Bottle BDH4504-1KGP HEPES g Poly Bottle BDH GP HEPES kg Poly Bottle BDH4518-1KGP HEPES Sodium Salt g Poly Bottle BDH GP HEPES Sodium Salt kg Poly Bottle BDH4520-1KGP Magnesium Acetate Tetrahydrate g Poly Bottle BDH G Magnesium Acetate Tetrahydrate kg Poly Bottle BDH4170-1KG Magnesium Chloride Hexahydrate g Poly Bottle BDH G Magnesium Chloride Hexahydrate g Poly Bottle BDH G MES Monohydrate g Poly Bottle BDH GP MES Monohydrate kg Poly Bottle BDH4544-1KGP MOPS g Poly Bottle BDH GP MOPS kg Poly Bottle BDH4522-1KGP PIPES g Poly Bottle BDH GP PIPES kg Poly Bottle BDH4546-1KGP PIPES kg Poly Pail BDH4546-5KGP Potassium Chloride g Poly Bottle BDH GP Potassium Chloride kg Poly Bottle BDH4532-1KGP Polysorbate L Poly Bottle BDH4530-4LG

5 Primary Antibodies Rockland produces an extensive range of Antibodies and Antibody Based Tools in its laboratories located west of Philadelphia, PA. Primary antibodies are in the areas of Apoptosis, Cell Signaling, Cytokines, Notch Signaling Pathway, NFkB Pathway, PI3 Kinase Pathway, Ubiquitin & UBL Pathway PRODUCT NAME HOST APPLICATIONS SIZE CAT. NO. Anti-ABCB5 (Rabbit) WB, IP, E 100 µg RL600401A77 Anti-AKT pan (Rabbit) IHC, IF, WB, IP, E 200 µl RL Anti-AKT Phospho ps473 (Mouse Mab) IHC, IF, WB, IP, E 100 µg RL Anti-AKT Phospho pt308 (Mouse Mab) IHC, IF, WB, IP, E 100 µg RL Anti-Angiopoietin 1 (Rabbit) WB, IP, E 200 µl RL Anti-Apolipoprotein A-I (Goat) IHC, WB, E 1 mg RL Anti-ATM Protein Kinase ps1981 (Mouse Mab) IHC, IF, WB, IP, E 100 µg RL Anti-ATM Protein Kinase S1981 (Rabbit) WB, IP, E 100 µg RL Anti-Beta Amyloid (Rabbit) IHC, IF, WB, E 100 µg RL Anti-Collagen Type I (Rabbit) IHC, WB, E 100 µg RL Anti-COX 2 (Rabbit) WB, IP, E 100 µl RL Anti-CREB-1 (p43) (Rabbit) WB, IP, E 100 µl RL Anti-Dab1 ( ) (Rabbit) IHC, WB, IP, E 100 µl RL Anti-DELTA-4 (Rabbit) IHC, WB, IP, E 100 µg RL Anti-Gli1 (Rabbit) WB, IP, E 100 µl RL Anti-Heat Shock Protein 27 (HSP27) (Mouse Mab) IHC, WB, IP, E 100 µg RL Anti-hTERT (Rabbit) IHC, IF, WB, IP, E 100 µg RL Anti-Interleukin 6 (IL-6) (Rabbit) IHC, IF, WB, IP, E, RIA, N 1 ml RL Anti-IKB alpha C-terminal (Rabbit) WB, IP, E 100 µl RL C Anti-Jagged-1 (Rabbit) IHC, IF, WB, IP, E 500 µg RL Anti-Mcl 1 (Rabbit) WB, IP, E 100 µg RL Anti-Mesothelin, Clone MB (Mouse Mab) IHC, WB, IP, E 100 µg RL200301A87 Anti-Myosin ps19/ps20 (Rabbit) IHC, IF, WB, IP, E 100 µg RL Anti-Nestin (Rabbit) WB, IP, E 100 µg RL Anti-NFKB p65 (Rel A) (Rabbit) WB, GS, IP, E 100 µl RL Anti-NOTCH 1 (Cleaved N terminal) (Rabbit) WB, IP, E 200 µl RL Anti-Osteopontin (Rabbit) IHC, WB, IP, E 200 µl RL Anti-Ovalbumin (Rabbit) IHC, WB, IP, E 50 mg RL Anti-Pdcd4 (Programmed Cell Death 4) (Rabbit) IHC, WB, IP, E 100 µg RL Rb anti-akt Ms anti-atm ps1981 Rb anti-cox 2 Rb anti-dab1 Rb anti-jagged-1 Rb anti-akt ps473 Rb anti-collagen I Rb anti-beta Amyloid Rb anti-delta4 Rb anti-mesothelin MN Anti-PPAR alpha (N-terminal specific) (Rabbit) WB, IP, E 100 µg RL Anti-SMAD3 ps423 / ps425 (Rabbit) IHC, WB, IP, E 100 µg RL Rb anti-myosin ps19/20 Rb anti-nrf 1 Anti-STREPTAVIDIN (Rabbit) WB, IP, E 50 mg RL Anti-SUMO Yeast (Rabbit) IHC, WB, IP, E 500 µg RL Anti-TNF alpha (Rabbit) IHC, WB, IP, E 1 mg RL Anti-Toll Like Receptor 2 (TLR2) (Rabbit) IHC, WB, IP, E 100 µg RL Anti-UBIQUITIN (Rabbit) WB, IP, E 500 µg RL Rb anti-pdcd4 Rb anti-smad3 ps423/425

6 Conjugated Secondary Antibodies Host Fluorescein DyLight 680 IRDye 800 Peroxidase Alkaline Phosphatase A=495 / E=528 A=682 / E=715 A=770 / E=794 HRP Alk. Phos. Anti-Chicken IgY Rabbit RL RL RL RL RL mg 100µg 500µg 1.5mg 1.0mg Anti-Goat IgG (H&L) Rabbit RL RL RL RL RL Anti-Goat IgG (H&L) Donkey RL RL RL RL RL Anti-Goat IgG Fc Rabbit RL RL RL RL RL Anti-Guinea Pig (H&L) Goat RL RL RL RL RL (Min X Bv Ch Gt Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) 1.0mg 100µg 500µg 1.0mg 1.0mg Anti-Hamster IgG (H&L) Goat RL RL RL RL Golden Syrian & Armenian 1.0mg 100µg 500µg 1.0mg Anti-Human IgG (H&L) Goat RL RL RL RL RL (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins) 1.0mg 100µg 500µg 1.0mg 1.0mg Anti-Mouse IgG (H&L) Rabbit RL RL RL RL RL Anti-Mouse IgG (H&L) Goat RL RL RL RL RL (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) 1.0mg 100µg 500µg 1.0mg 1.0mg Anti-Mouse IgG1 Rabbit RL RL RL RL (gamma 1 subclass specific) 1.0mg 100µg 1.0mg 1.0mg Anti-Mouse IgG2a Rabbit RL RL RL RL RL (gamma 2a chain subclass specific) 1.0mg 100µg 500µg 1.0mg 1.0mg Anti-Mouse IgG Fc Goat RL RL RL RL RL mg 100ug 500µg 2.0mg 1.0mg Anti-Mouse IgM (mu chain) Goat RL RL RL RL RL mg 100µg 500µg 1.0mg 1.0mg Anti-Rabbit IgG (H&L) Goat RL RL RL RL RL Anti-Rabbit IgG Fc Goat RL RL RL RL RL Anti-Rat IgG (H&L) Rabbit RL RL RL RL RL Anti-Sheep IgG (H&L) Rabbit RL RL RL RL RL Anti-Sheep IgG (H&L) Donkey RL RL RL RL RL (Min X Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins) 1.0mg 100µg 500µg 1.0mg 1.0mg STREPTAVIDIN RLS RLS RLS RLS RLS mg 100µg 500µg 1.0mg 1.0mg Anti-alpha-Tubulin Anti-beta-Actin Anti-GAPDH Rabbit Rabbit Rabbit LOADING CONTROL ANTIBODIES RL µg RL µg RL A33 100µg DyLight is a trademark of Thermo Fisher Scientific FLAG is a registered trademark of Sigma-Aldrich IRDye is a registered trademark of LI-COR, Inc Rb anti-gapdh Rb anti-beta-actin Rb anti-alpha-tubulin

7 Green Fluorescent Protein (GFP) & Red Fluorescent Protein (RFP) Antibodies Rockland s GFP and RFP antibody conjugates provide the researcher with unmatched Level of Detection (LOD) brightness and photostability. Anti-GFP antibodies are ideal for detecting wild type, recombinant and enhanced forms of GFP & RFP. They are also suitable for signal amplification methods in cells or tissues. Applications: Western Blotting, Immunohistochemistry, Fluorescence Microscopy, ELISA, Odyssey Infrared Imaging System. Anti-GFP (Green Fluorescent Protein) Host: Goat Unconjugated 1.0mg RL Fluorescein 1.0mg RL DyLight µg RL Biotin 1.0mg RL Peroxidase 1.0mg RL IRDye µg RL Anti-RFP (Red Fluorescent Protein) Host: Rabbit Unconjugated 100µg RL Fluorescein 100µg RL Biotin 100µg RL Peroxidase 100µg RL Anti-GST & Anti-DYKDDDDK Antibody detects GST and FLAG epitope tags Rockland s Antibodies to GST and Epitope Tags can recognize antigenic determinants (epitopes) on recombinant proteins and other synthetic constructs. The most commonly used epitope tags include His tag (6-Histidine), HA tag (Hemagglutinin), c- Myc tag, GST tag, and DYKDDDDK. Rockland offers highly specific antibodies to all of these epitope tags. Applications: Western Blot, Immunohistochemistry, FLOW Cytometry, Immunocytochemistry, Fluorescence Microscopy, Immunoprecipitation (IP), ELISA. Anti-GST (Glutathione S-Transferase) Host: Goat Unconjugated 1.0mg RL Agarose 20mg RL Biotin 1.0mg RL Peroxidase 1.0mg RL DYKDDDDK Antibody detects FLAG Tag Host: Mouse Unconjugated 100µg RL Agarose 1.0ml RL Biotin 100µg RL Peroxidase 100µg RL Anti-Dye, Anti-Hapten Antibodies and More Anti-6X HIS (Rabbit) 100µg RL Anti-CAT (Rabbit) 100µl RL Anti-Beta Galactosidase (Rabbit) 10mg RL Anti-DNP (Hamster) 100µg RL200-B Anti-GFP (Mouse) 1.0mg RL Anti-HA (Rabbit) 100µg RL Anti-Biotin (Goat) 1.0mg RL

8 Western Blotting Kits & Chemiluminescent Substrate Rockland's FemtoMax Super Sensitive Chemiluminescent Western Blot Kit combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol substrate. Kit design includes straightforward, easy-to-use procedures and color-coding. Optimized reagents provide for the highest signal-to-noise ratio available. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm 2 (2,400 cm 2 ). The kit allows for the detection of primary polyclonal antibodies provided by the user. Rockland s specialized formulation of luminol provides an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Western Blot Kit for use with Rabbit Primary Antibody 1 Kit RLKCA003 Western Blot Kit for use with Mouse Primary Antibody 1 Kit RLKCA002 Chemiluminescent Substrate FemtoMax detection of femtogram (10-15 ) 110ml RLFemtoMAX-110 Blocking Buffers Rockland s blocking solution are especially useful in Western Blotting, ELISA and Immunohistochemistry (IHC) procedures for reducing background staining. Blocking solutions bind to any open protein binding sites preventing non-specific protein binding. Bovine Serum Albumin (BSA) 50g RLBSA50 30% Bovine Serum Albumin Solution 500ml RLBSA-30 Goat Serum (NGS) 10ml RLB304 ELISA Microwell Blocking Buffer and Stabilizer 1000ml RLMB Fluorescent Western Blotting Blocking Buffer 500ml RLMB-070 Agarose Conjugates Antibody affinity gels are designed for immunoprecipitation (IP) of antigens from sera, antisera, ascitic fluid or culture fluid, bacterial and mammalian cell lysates and fusion protein preparations and are also useful for selection of immunoglobulins or removal of unwanted cross-reactivity PRODUCT NAME HOST SIZE CAT. NO. Anti-Goat IgG Rb 20mg RL Anti-Mouse IgG Gt 20mg RL Anti-Rabbit IgG Gt 20mg RL SEPHAROSE Protein A 2.0ml RLPA ml RLPA ml RLPA IgGy Antibody Selector Use IgGy - VWR s Antibody Selector Tool Cell Signaling Apoptosis Cancer Research Chromatin Neuroscience Ubiquitin Secondary Antibodies Epitope Tags M Lit. No FOR INFORMATION OR TO PLACE AN ORDER, CONTACT VWR AT OR GO TO