Product Datasheet. CD31/PECAM-1 Antibody (C31.7) NBP mg. Unit Size: 0.1 mg. Store at 4C. Publications: 1

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1 Product Datasheet CD31/PECAM-1 Antibody (C31.7) NBP mg Unit Size: 0.1 mg Store at 4C. Publications: 1 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 7/11/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NBP mg CD31/PECAM-1 Antibody (C31.7) Product Information Unit Size Concentration 0.1 mg 0.2 mg/ml Storage Store at 4C. Clonality Monoclonal Clone C31.7 Preservative Isotype Purity Buffer 0.05% Sodium Azide IgG1 Kappa Protein A or G purified 10mM PBS and 0.05% BSA Page 1 of 5 v.20.1 Updated 7/11/2018 Product Description Host Mouse Gene ID 5175 Gene Symbol Species Reactivity Notes Marker Specificity/Sensitivity Immunogen Product Application Details Applications Recommended Dilutions PECAM1 Human, Cynomolgus Monkey, Rabbit Human, Cynomolgus Monkey and Rabbit. Weakly reacts with Rat. Feedback has been negative on pig kidney paraffin sections. Endothelial Cell Marker CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence. Human recombinant CD31 protein Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, SDS-Page Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry- Paraffin 0.5-1ug/ml, SDS-Page Application Notes Human Chromosome Location: 17q23.3 Mol. Weight of Antigen: ~100kDa (endothelium) and ~130kDa (platelets) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, ph 8.0, for min followed by cooling at RT for 20 min. Immunocytochemistry/Immunofluorescence application cited in literature.

3 Images Western Blot: CD31/PECAM-1 Antibody (C31.7) [NBP ] - Analysis using the Azide and BSA Free version of NBP Detection of CD31 in THP-1 lysate using CD31 MAb (C31.7) Page 2 of 5 v.20.1 Updated 7/11/2018 Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.7) [NBP ] - Analysis using Azide/BSA FREE version of NBP Formalin-paraffin human Angiosarcoma stained with CD31 MAb (C31.7). Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP ] - Human CD31 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled CD31 monoclonal antibody (C31.7). Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.7) [NBP ] - Analysis using the Azide and BSA Free version of NBP Staining of human Tonsil stained with CD31 MAb (C31.7).

4 Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP ] - Human CD31 on Jurkat Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD31 monoclonal antibody (C31.7). Page 3 of 5 v.20.1 Updated 7/11/2018 SDS-Page: CD31/PECAM-1 Antibody (C31.7) [NBP ] Publications O'Connor M, Patil R, Yu J et al. Mesenchymal Stem Cells Synergize with 635, 532, and 405 nm Laser Wavelengths in Renal Fibrosis: A Pilot Study. Photomed Laser Surg. [PMID: ] Details: Citation using the Azide and BSA Free form of this antibody.

5 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NBP mg HAF007 NB720-B NBP mg NBP PEP Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] (Preadsorbed) Mouse IgG1 Kappa Light Chain Isotype Control (P ) CD31/PECAM-1 Recombinant Protein Antigen Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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