Dayanand College, Hisar

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1 Dayanand College, Hisar Session Lesson Plan for B.Sc. Vth Semester (Theory) Name of Assistant Professor:Mr. Lalit Kumar (Deptt.of Biotechnology, Dayanand College, Hisar) Class: B. Sc. 3 rd Year (5 th Sem.) Subject: Paper XI. Animal Biotechnology Subject Lesson Plan: Odd Semester 20 weeks (from July 2018 to November 2018) Date Topic july Animal Cell & Tissue Culture: Introduction, Principles & practice. History and Development of animal cell culture. Scope and Applications August Culture Media: Media components, Serum containing and serum free media. Natural media- Plasma clot, biological fluids, tissue extracts August Growth factors required for proliferation of animal cells. Chemically defined media, balanced salt solutions August Physical requirements for growing animal cells in culture. Washing, drying, sterilization practices, various instruments and their uses in animal cell culture practices August Primary Cell Culture techniques: Initiation of cell culture-substrates (glass, plastic, metals) their preparation and sterilization August Isolation of tissue explants, disaggregation- enzyme disaggregation and mechanical disaggregation of the tissue. development of primary culture and cell lines September Subculture. Contamination.. Suspension culture, Growth curve of animal cells in culture. Secondary cell culture transformed cell and continuous cell lines. Finite and infinite cell lines. Cell lines: Insect and animal cells September Commonly used cell lines- their organization and characteristics. Cell repositories and their function. Karyotyping, biochemical and genetic characterization of cell lines.

2 20-22 September Organ Culture: technique, advantages, applications and limitations. Artificial skin September Transfection of animal cells: transfection methods. Methods for cell fusion, Selectable markers, HAT selection and Antibiotic resistance. 27 September Assignment submission October Cloning and expression of foreign genes in animal cells: Expression vectors. Over production and preparation of the final product i.e. expressed proteins. Production of vaccines in animal cells October Hybridoma Technology: Production of monoclonal antibodies and their applications 18 October Assesment Test October Embryo transfer technology- technique, its applications. Artificial insemination. Animal clones. Transgenic Animals: transgenic sheep, cow, pig, goat October Production of transgenic mice, ES cells can be used for gene targeting in mice, applications of gene targeting. Therapeutic products through genetic engineering blood proteins, insulin, growth hormone etc November Gene Therapy: introduction, types of gene therapy, vectors in gene therapy, major achievements, problems and prospects November Doubt classes Dr. Vivek Srivastava (Associate Professor) Head, Dept. of Biotechnology Mr. Lalit Kumar (Assistant Professor)

3 DAYANAND COLLEGE, Hisar Department of Biotechnology Lesson Plan Semester III (M. Sc. 2) year Paper BT-116 SUBJECT: Microbial Biotechnology Sr. No. Date Topics 1 Aug 1to Aug 7, 2018 Microbial Biotechnology: Scopes application and challenges. Isolation preservation and improvement of industrially important microorganisms. 2. Aug 8, 2018 Revision work 3 Aug 9to Aug 14, 2018 Kinetics of microbial growth and product formation. Fermentation system; batch and continuous system 5 Aug 16 to Aug 23, 2018 fed batch system, multistage system. Solid state fermentation. Overproduction of primary and secondary metabolites 6 Aug 24,2018 Problem solves 7 Aug25 to Aug31, 2018 Fermentation raw materials: Media for industrial fermentations; criteria used in media formulation. Fermenter/bioreactor design and operation; types of fermentor, stirred tank reactor, 8 Sep Seminars taken 9 Sep 2 to Sep 9, 2018 bubble column reactor, airlift reactor, packed bed reactor, fluidized bed reactor and trickle bed reactor, agitation and aeration in a reactor, mass transfer. Foam formation and control.. 10 Sep 10, 2018 Seminars taken

4 11 Sep11 to Sep 17, 2018 Industrial production of alcohol (ethanol, wine and beer) and improvement by genetic engineering. 12 Sept 18,2018 Assignments given 13 sep 19 to 25Sep, 2018 Microbial production of acids (citric, acetic and gluconic acid) solvents (glycerol acetone and butanol) 14 Sep 26,2018 Assessment test 15 Sep 27 to 6oct, 2018 Assignments submission 16 Oct 8 to 12 Oct,2018 aminoacids (lysine and glutamic acid).production of antibiotics ; Penicillin and cephalosporin 17 Oct 13 to 19 Oct,2018 Microbial polysaccharides: fermentative production of xanthan gums,. Bacterial bioplastics, genetic engineering of microorganisms for the production of poly-3 hydroxyalkanoates. Microbial inoculants: Food starter cultures; 18 Oct 22 to 29 Oct,2018 baker s yeast, starter cultures for the dairy industry, meat starter cultures Biomass production: single cell protein (SCP) production; microbial inoculants; Microbial transformation of steroids and sterols 19 Oct 30 to 9 Nov,2018 Revision 20 Nov 9 to 15 Nov,2018 Doubt Classes Dr. Vivek Srivastava (Associate Professor) Head, Dept. of Biotechnology Mr. Lalit Kumar (Assistant Professor)

5 Dayanand College, Hisar Session Lesson Plan for B.Sc. III rd Semester (Theory) Name of Assistant Professor:Mr. Lalit Kumar (Deptt.of Biotechnology, Dayanand College, Hisar) Class: B. Sc. 2 nd Year (3rd Sem.) Subject: Paper VII. Molecular Biology Subject Lesson Plan: Odd Semester 20 weeks (from July 2018 to November 2018) Date Topic July Molecular Biology: Introduction to molecular aspects of life. DNA as the genetic material experiments proving DNA and RNA as genetic material. Nucleic acids: Structure, function and properties of DNA and RNA July Watson and Crick model of DNA. DNA forms (A, B and Z), their characteristic. Different types of RNA, their structure and function August Organization of Genomes bacterial, viral, human, organelles. Eukaryotic genomes: Chromosomal organization and structure August Euchromatin, heterochromatin, centromere, telomere. Chromatin structure (nucleosome), histone and non-histone proteins August Insertion elements and transposons; IS elements, transposable elements of Maize and P elements of Drosophila. Extra chromosomal DNA in prokaryotes plasmids August DNA Replication: Central dogma of molecular biology. Semiconservative mode of DNA replication, experimental proof. Unidirectional and bidirectional mode of DNA replication September Theta model and rolling circle model. DNA replication in prokaryotes and eukaryotes, different stages, proteins and enzymes involved September DNA damage and repair: causes of DNA damage, mutations. Repair mechanisms- photo reactivation, excision repair, mismatch repair, SOS repair

6 17-19 September September Genetic Code: concept, elucidation or cracking of genetic code, features of genetic code, Wobble hypothesis. Structure of gene- introns/exons, regulatory sequences, structure of prokaryotic gene October Transcription in prokaryotes and eukaryotes, diff. stages, mechanism, promoters, transcription factors, RNA polymerases 08 October Assignment submission October Post transcriptional modifications- 5 cap formation, 3 -end processing/polyadenylation and gene splicing and generation of mature mrna. Inhibitors of transcription. 15 October Assesment Test October Translation/Protein synthesis: Mechanism of initiation, elongation and termination of protein synthesis in prokaryotes and eukaryotes October Inhibitors of translation. Post-translational modifications. Regulation of Gene Expression in prokaryotes and eukaryotes, induction and repression, positive and negative regulation October Operon model- lac, ara, trp, catabolite repression, transcription attenuation November Molecular mechanisms of DNA recombination in eukaryotes Site Specific and Homologous recombination. Recombination in prokaryotes Transformation, transduction and conjugation November Doubt classes Dr. Vivek Srivastava (Associate Professor) Head, Dept. of Biotechnology Mr. Lalit Kumar (Assistant Professor)

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