B.Sc V Semester Question Bank. B.Sc V Semester Question Bank. Prepared by Nitin Swamy, Department of Biotechnology, SACJ Page 1 of 6

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1 B.Sc V Semester Question Bank Prepared by Nitin Swamy, Department of Biotechnology, SACJ Page 1 of 6

2 UNIT I Genetic Material Different forms of DNA (DNA topology):- B-form, Z-form, D-form; Gene structure-introns,exons and pseudogenes: Supercoiling of DNA, Melting curve of DNA and Tm value determination; Buoyant density of DNA and its relationship with mole (G+C) content in DNA; Types of RNA: mrna, rrna, trna; 1. What is B-form of DNA? 2. Write a note on gene structure? 3. Write a note on supercoiling of DNA? 4. What is Melting curve of DNA and Tm value? 5. What are pseudogenes? 1. Explain different types of RNA? 2. Explain exons and introns? 3. Explain the organization of eukaryotic genome? 4. Write a note on different forms of DNA? 5. Explain the buoyant density of DNA and its relationship with mole (G+C) content in DNA? Prepared by Nitin Swamy, Department of Biotechnology, SACJ Page 2 of 6

3 UNIT II DNA Replication Types of DNA replication: Conservative, Semi-conservative and Dispersive mode of replication; Mechanism of replication, Messelson and Stahl experiment; Enzymes involved in replication of DNA; Replication of DNA in prokaryotes and eukaryotes, Models of chromosomal replication- Cairns model,rolling Circle model. 1. Write a note on semi conservative mode of DNA replication? 2. Write a note on Messelson and Stahl experiment? 3. Write a note DNA polymerase? 4. Write a note Cairns model of replication. 5. Write a note Okazaki fragment. 1. Explain the DNA replication in eukaryotes? 2. Explain the DNA replication in prokaryotes? 3. Write a long note on the chromosomal replication model? 4. Write a long note on enzymes used in the DNA replication? 5. Draw a neat diagram of general model of replication. Prepared by Nitin Swamy, Department of Biotechnology, SACJ Page 3 of 6

4 UNIT III Transcription, Translation and Gene regulation The Relationship between genes and protein (Central Dogma) The Transcription in Prokaryotic Cells: The basic process of Transcription, mrna Processing (Post Transcriptional modification). Translation to protein Role of ribosome in protein synthesis, process of translation in prokaryotic cells. The Genetic Code and its basic features, Wobble Hypothesis. Regulation of Protein Synthesis: Lac Operon, Trp Operon. 1. Write note on Lac operon? 2. What is wobble hypothesis? 3. What is the function of ribosome? 4. Write a note on the elongation of transcription in eukaryotic? 1. Write a long note on transcription? 2. Write a long note on translation? 3. Write a note on basic features of genetic code. 4. Explain the post translational modification. Prepared by Nitin Swamy, Department of Biotechnology, SACJ Page 4 of 6

5 UNIT IV DNA mutation and repair Types of mutation; evidence of spontaneous nature of mutation: Luria and Delbruck fluctuation test and replica testing; mode of action of physical, chemical and biological mutagens-uv rays, nitrous acid, 5- bromouracil, 2-aminopurin, EMS. Reversion in mutation, suppression and types of suppressor mutation; DNA repair mechanism, Photo reactivation, Excision, Mismatch, SOS repair. 1. Write a note on Luria and Delbruck fluctuation test? 2. What is replica plate method? 3. Write mode of action of physical mutagens 4. Write mode of action of chemical and biological mutagens? 5. What is reversion in mutation? 6. What is photo reactivation DNA repair? 1. Write a long note on types of mutation? 2. What is suppression mutation and types of suppressor mutation 3. Explain the DNA repair mechanism in general? 4. Write in detail about Base excision and nucleotide excision repair? 5. What is mismatch repair? Explain with neat diagrams. Prepared by Nitin Swamy, Department of Biotechnology, SACJ Page 5 of 6

6 UNIT V Genetic engineering Recombinant DNA Technology- Definition, principle, Molecular tools of genetic engineering: Cutting and joining of DNA, Cloning Vectors, Techniques of genetic engineering, Gene librarygenomic library and cdna library. Application of PCR technology (Diagnosis of pathogen, DNA fingerprinting.) Basics of Site Directed mutagenesis and protein engineering. 1. What is side directed mutagenesis? 2. What is genomic library? 3. What is cdna library? 4. What are plasmid vectors? 5. What are endonucleases and exonuclease? 1. What is recombinant DNA technology? Explain the molecular tool of genetic engineering? 2. Write a note on enzymes used in recombinant DNA technology? 3. What is PCR? Explain the applications of PCR? 4. What is protein engineering? 5. What is vector? Explain the characteristics of a good vector taking PBR322 as an example? Note for previous years question papers, visit the study resources page in this blog to get a better idea of question asked in examination. Prepared by Nitin Swamy, Department of Biotechnology, SACJ Page 6 of 6