BIO 343 INTRODUCTION TO GENETICS LECTURER: Dr. Lisa Dorn

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1 BIO 343 INTRODUCTION TO GENETICS LECTURER: Dr. Lia Dorn OFFICE: HS 45; LAB: HS 47/50 PHONE: ; OFFICE HRS: Poted to D2L LECTURE HOURS: 11:30 12:30 pm in Haley Science Building; Rm.268 TEXT: Brooker, Genetic: Analyi & Principle (4th edition or Cutom Book) McGraw Hill. OBJECTIVES: Genetic and it experimental method i one of the broadet and mot rapidly evolving field of cience. I cannot poibly cover it all, o my goal i to teach you how to think like a geneticit, while learning genetic concept o that you can judge for yourelf the value of emerging genetic technologie and dicoverie. Thi coure cover Mendelian genetic and it complication, the molecular bai of gene and their affect on phenotype, the method of identifying and characterizing the genetic bai of dieae and other phenotype a well a population and evolutionary genetic. The prerequiite for thi coure are Bio105 and 323, which by extenion mean you have taken Chem 105 and 106. The laboratory part of thi coure will expand on what you have learned in Molecular and Cell Biology (Bio 323). I will aume you have matered the Bio323 material and will not wate much time reviewing thi material but there will be ome review of 323 material in lab. ASSESSMENT: How are you going to earn your grade? EXAMS: 4 exam each worth 100 point. They will be motly problem imilar to thoe in your D2L quizze. (400 total point). Note: For exam you will need a calculator. YOU CANNOT USE YOUR PHONE AS A CALCULATOR ON EXAMS. PROBLEM SOLVING: There will be 8 D2L problem-olving eion where you will be required to olve problem that will be available on D2L. They are worth 10 point each (total = 80 point). Thee problem are preented a quizze that will be available ~1 week before the due date for that quiz. During that week you may open it, print it and dicu potential olution with your peer and me. On the day that the quiz i due you have until 10:30pm to ubmit your quiz. After that 10:30 pm time you have a 6 hr grace period to ubmit late (i.e. until 5:30 am the following morning). After that, you loe 1 pt for every hour the quiz i late. THE QUIZ CLOSES AT 10:30PM THE DAY AFTER IT IS DUE AT WHICH POINT THE GRACE PERIOD IS OVER! You can make up ome or all of thoe lot point at the end of the emeter with the extra credit quiz depending on how many quizze you mi.

2 You hould ave the anwer a you enter them. You can till change anwer even after aving but once you ubmit the quiz you cannot. You can ee the anwer 1 day after the late ubmiion deadline. ***IMPORTANT***: D2L can be temperamental. Do not wait for the lat hour to ubmit your anwer. D2L tell me when you are logged on, if you tried to ubmit a quiz and even if you have opened the quiz. Excue for waiving late ubmiion penaltie that will NOT be accepted include: D2L problem in the lat hour before the deadline. I forgot to ave my anwer I forgot after I went to work My internet ervice at home failed. BUT if you have ubmitted by the deadline and D2L fail to recognize your ubmitted quiz end me an . In mot cae, I have your quiz and can force D2l to accept it. EXTRA CREDIT QUIZZES: There will be one extra credit quiz at the end of the emeter worth 5 point. Beide the extra credit, it will be very imilar to quetion you will find on the lat exam o I highly recommend that you do thi. *NOTE: Extra credit point from thi quiz can be ued to pa the coure but, you may have other opportunitie for extra credit point that may change your letter grade but thee EC point cannot be ued to pa the coure. LABORATORY: I worth 105 point. The lab yllabu i appended to the end of thi document. LABORATORY ATTENDANCE: Loe 5 pt on a report for every unexcued abence during the week of that report exercie. Total Poible point = = 585. GRADING SCALE: A = %, A- = % B+ = % B = % B- = % C+ = % C = % C- = % D+ = % D = % D- = % F (Failure) < 60% Grade may be curved at the end, if neceary.

3 Diputing a grade: if you feel your exam ha been migraded, you mut ubmit to me a requet for re-grade within a week of the day I have paed exam back (not the day you picked it up). That requet mut be in writing accompanied by a copy of the exam in quetion that I will keep. Student With Diabilitie are welcome in thi cla! If you need pecial accommodation pleae contact me during office hour in the firt week of cla. Thi include tudent with diagnoed learning diabilitie. If you feel you hould be evaluated for a learning diability, pleae contact the Project Succe office at or go to their webite at Claroom Etiquette: pleae ilence all pager, cell phone; or ipod etc.and do not talk or whiper unle called upon in turn (but feel free to raie your hand for a quetion or comment at ANY time!). Pleae do not text during my lecture. If you find me that boring jut don t bother to come. Texting i more diruptive than you upect and wate both my time and your money. Incomplete Grade may be given in extreme circumtance, uch a when a tudent become too ill to complete the emeter' work. Pleae talk to me if you think your ituation warrant an "I" grade and be prepared to provide documentation. Make up exam. If you cannot make one exam everal alternative may be available to you depending on the ret of my teaching obligation at that time. I will do what I can, given your circumtance. If you know ahead of time that you mut mi an exam (for intance if your job require that you work that day) pleae let me know ahead of time. The chedule of lecture, exam and due date for problem. There may be minor change to thi chedule of lecture without notice. I will announce uch change in cla. Exam and quizze will tay on chedule unle (for quizze) there are problem with D2L. Lec # Day Date Topic Book 1 Wed 5-Sep Mendel Law; Monohybrid & Dihybrid Ch2 2 Fri 7-Sep Dihybrid & Pedigree Ch2 3 Mon 10-Sep Pedigree; Probability Ch2 4 Wed 12-Sep Chi-Square Chromoome Sex-Linkage Ch 3 5 Fri 14-Sep Sex Determination; Meioi Ch 3 Quiz 1 Due 6 Mon 17-Sep Receive Lethal Ch 4

4 7 Wed 19-Sep Pleiotropy; Incomplete Dominance Ch 4 8 Fri 21-Sep Co-Dominance, Overdominance, Ch 4 9 Mon 24-Sep Penetrance, Complementation Ch 4 10 Wed 26-Sep Epitai, Linkage and Recombination Quiz 2 Due Fri 28-Sep EXAM 1 (cover up to lecture 9) Ch 4/6 11 Mon 1-Oct Linkage and Recombination Ch 6 12 Wed 3-Oct Mapping gene: dihybrid croe Ch 6 13 Fri 5-Oct Mapping gene: Trihybrid croe Ch 6 Quiz 3 Due 14 Mon 8-Oct Mapping gene: Trihybrid croe Ch 6 15 Wed 10-Oct Genomic Ch Fri 12-Oct Genomic Ch Mon 15-Oct Genomic Quiz 4 Due Wed 17-Oct EXAM 2 (covering lecture 10 to 18) Ch Fri 19-Oct Gene Mutation Ch Mon 22-Oct Gene Mutation Ch Wed 24-Oct Chromoome: Change in Number Ch Fri 26-Oct Chromoome: Structure; Variation & Mutation Ch Mon 29-Oct Chromoome: Polyploidy Ch Wed 31-Oct Trancription & Pot-Trancriptional Proceing Ch 12 Quiz 5 Due 24 Fri 2- Trancription & Pot-Trancriptional Proceing Ch Mon 5- Pot-Trancriptional Proceing Ch Wed 7- Pot-Trancriptional Proceing Ch Fri 9- Trancriptional Regulation in Eukaryote Ch 15

5 28 Mon 12- Trancriptional Regulation Chromatin & RNAi Ch Wed 14- Trancriptional Regulation Chromatin & RNAi Ch5 Quiz 6 Due 30 Fri 16- Non-Mendelian Inheritance: X-inactivation Ch5 Mon 19- 'EXAM 3 ( Lecture 19 to 29) Wed 21- Thankgiving Break (NO LABS THIS WEEK) Fri 23- Thankgiving Break(NO LABS THIS WEEK) 31 Mon 26- Non-Mendelian Inheritance: Imprinting Ch5 32 Wed 28- Non-Mendelian Inheritance: X-inactivation & Ch5 Imprinting 33 Fri 30- Population Genetic: HW Ch Mon 3-Dec Population Genetic:Drift Ch 24 Quiz 7 due 35 Wed 5-Dec Population Genetic Variation Ch Fri 7-Dec Population Genetic Inbreeding Ch Mon 10- Dec Wed 12- Dec Fri 14- Dec Population Genetic/ Review Quiz 8 Due; Ch 24 EC Quiz Due EXAM 4 (Lecture 30-37) Ch 24 Alternate Exam day Genetic Lab Syllabu Intructor: Lia Dorn HS 45/47/50; dorn@uwoh.edu Section A01L: 9:40am 11:40 am in HS 50, Section A02L: 1:20 pm 3:20 pm in HS 50, Intructor: Roemary Shade HS 142; hade@uwoh.edu Section A03L: 9:40 11:40 am in HS 50 OBJECTIVES: Welcome to the laboratory portion of Biology 343. Thi coure i deigned to give you hand-on experience with modern genetic technique. It i not deigned to coincide very much with your lecture material but it will on occaion. You will learn molecular lab technique that are ueful for genetic, learn to debug thoe

6 technique, a well a how to interpret your reult and what that mean for undertanding gene and their function. Thee exercie are baed on molecular biology concept you hould have learned in 105 and 323. Therefore, we will ue ome lab time to review thoe molecular biology concept. Depite thi coure poition a a core coure there are only two hour a week devoted to laboratory. That mean that your exercie will tretch out over a period of week. We will help you keep track. It may help if you ue the chedule preented below a a checklit. ORGANIZATION: The coure conit of three baic ection or module. Within each module are a erie of exercie deigned to teach the topic covered by that module. The firt module Baic Lab Technique teache the baic technique you will need for the ret of the emeter. For ome of you with experience in profeor lab thi module will be very baic at time. Pleae be patient. Remember thi coure i a required coure for all biology major even thoe who are not puruing reearch that require thee technique. Thoe of you with reearch experience may find that uing your expertie to help le-experienced tudent a ueful exercie. Lat, ome of thee exercie are deigned to at leat partially fail o do not get dicouraged. GRADING: The lab part of the coure i worth 105 point toward your total grade. There are four quizze and five report a hown in the table below. Quiz/ Report Point Quiz 1: Nucleic Acid Review 10 Quiz 2: Replication 10 Report 1: Electronic PCR 10 Quiz 3: Optimization/ Polymorphim 20 Report 2: Polymorphim 15 Report 3: Marker Map 10 Quiz 4: Gene Expreion 10 Report 4: RT-PCr 10 Report 5: Gene Familie 10 Total point 105

7 Date 4-Sep Wee k Due Date Module Wet Lab Exercie Computer Exercie Lab Manual Page Note 6-Sep 11-Sep 13-Sep 2 2 No Lab Baic Lab Technique Pipettor Exercie, Make Buffer 18-Sep 3 Quiz 1 Due 20-Sep 3 Quiz 1 Due Baic Lab Technique Extracting DNA 25-Sep 27-Sep 4 4 Baic Lab Technique: Quantify DNA Electronic PCR: Quiz 2 Available 2-Oct 4-Oct 5 5 Quiz 2 Quiz 2 Baic Lab Technique: PCR Optimization Electronic PCR: Set-Up reaction 9-Oct 11-Oct 6 6 Report 1: e-pcr Report 1: e-pcr Genomic Optimization Gel Polymorphim Screen

8 16-Oct 18-Oct 7 7 Genomic Polymorphim Gel Predict Polymorphi m 23-Oct 25-Oct 30-Oct Report 2: Polymorphim due Genomic Genomic Revere Trancription Draw Recombinant Predict Polymorphi m Work on Molecular Map; Handout mapping gel picture Quiz3: Opt/Poly Quiz3: Opt/Poly Genomic PCR part of RT-PCR Predict RT- PCR product Keep Working on Map Report 3: Marker Map Genomic RT-PCR gel Predict RT- PCR No Lab 12

9 Quiz 4: Gene Expreion Genomic Gene Familie Examine RT-PCR gel Genbank 4-Dec 6-Dec Report 4: RT- PCR Genomic Gene Familie 11-Dec 15 Report 5: Gene 13-Dec Familie 15 No Lab