BioWhittaker Specialty Media Maximize Your Cell Growth and Productivity

Transcription

1 BioResearch BioWhittaker Specialty Media Maximize Your Cell Growth and Productivity BioWhittaker Specialty Media Cell Culture Applications

2 BioResearch BioWhittaker Specialty Media Maximize Your Cell Growth and Productivity If your work leads to downstream therapeutic, diagnostic, or biopharmaceutical applications, serum-free media is the best choice initiating your research studies. BioWhittaker Specialty Media deliver outstanding permance, a broad selection, and consistent results, whether you need protein-free, non-animal (NAO) or chemically media. Our ISO 9000 and cgmp systems are recognized industry wide. BioWhittaker Serum-free Media deliver unique benefits: Consistent results Elimination of FBS lot qualification Elimination of FBS-borne mycoplasma and virus contamination Simplified downstream purification Reduced regulatory burdens Maximal yields General Purpose Media UltraCULTURE Serum-free Medium Delivers excellent growth and expansion of a broad range of adherent and suspension cells Simplify your media needs, no FBS needed The mulation UltraCULTURE Media has been submitted to the FDA as a product Master File PC-1 Defined, Serum-free Medium Superior media primary adherent cells HL-1 Defined, Serum-free Medium Unique media hybridoma and lymphocytic cells UltraCULTURE Medium n Most cell types PC-1 CD Medium n n Adherent primary cells and cell lines HL-1 CD Medium n Hybridomas and many adherent cell lines UltraCULTURE Medium 500 ml PC-1 CD Medium 2 X 500 ml HL-1 CD Medium 2 X 500 ml Mesenchymal Stem Cell Medium MSCGM CD Defined, Serum-free MSC Growth Medium multiple passage expansion of all types of hmscs Cells can be directly transitioned from serum-containing medium with little to no adaptation time No need attachment matrix to plate cells Supports multi-lineage differentiation Therapeak MSCGM CD Mesenchymal Stem Cell Defined Growth Medium BulletKit Kit Therapeak MSCBM CD Mesenchymal Stem Cell Defined Basal Medium 500 ml Therapeak MSCGM CD Mesenchymal Stem Cell Defined Growth Medium SingleQuots Kit 5 ml 2

3 Fibroblast Medium FGM CD Defined, Serum-free Fibroblast Growth Medium adult normal dermal fibroblasts and also supports neonatal cells Supports expression of fibroblast markers Ab5B5, CD90, and collagen I Collagen production is superior to that obtained with DMEM + FBS No weaning seamless transition from serum-containing media FGM CD Fibroblast Growth Medium Defined, BulletKit with L-glutamine, Without Phenol Red or Antibiotics Kit FBM CD Fibroblast Basal Medium Defined 500 ml FGM CD Fibroblast Growth Medium Defined, SingleQuots Kit Kit Cryopreservation Medium ProFreeze Defined Medium (2X), protein-free freezing medium Formulated cryopreserving all cell types Maintains high cell viability upon recovery from frozen storage Requires 15% DMSO at 2X ProFreeze Defined Medium (2X) 100 ml 3

4 BioResearch BioWhittaker Specialty Media Hematopoietic Media X-VIVO Defined, Serum-free Hematopoietic Cell Media Superior media most hematopoietic cell types including dendritic cells, lymphocytes, monocytes, macrophages, and granulocytes Some mulations include recombinant transferrin to meet the highest regulatory requirements All current X-VIVO media products are manufactured under current GMP's and are listed with the FDA in a product Master File. Highly cited in scientific literature X-VIVO 10 Medium PBL, LAK, monocytes, macrophages, stem cells X-VIVO 15 Medium n PBL, NK, TIL, monocytes, macrophages, stem cells, dendritic cells X-VIVO 20 Medium PBL, LAK, TIL, monocytes, macrophages, stem cells, dendritic cells X-VIVO 10 Medium With L-glutamine, Gentamycin and Phenol Red, With Human Transferrin 1 L X-VIVO 10 Medium With L-glutamine, Without Gentamycin or Phenol Red, With Human Transferrin 1 L X-VIVO 15 Medium With L-glutamine, Gentamycin, and Phenol Red, With Human Transferrin 1 L X-VIVO 15 Medium With L-glutamine, Without Gentamycin or Phenol Red, With Human Transferrin 1 L X-VIVO 20 Defined, Serum-free Hematopoietic Cell Medium, With L-glutamine, Gentamicin and Phenol Red 1 L 4

5 CHO Media PowerCHO Defined, Serum-free CHO Media PowerCHO Media bring new levels of cell proliferation and protein production to chemically media Maintain high viability (>90%) at high cell densities ProCHO CHO Media Multiple mulas to optimize your protein-free applications including adherent and suspension cells, with high proliferation rates and high protein yield Directly convert cultures from adherent with serum to suspension without serum PowerCHO Advance Medium, no raw materials of animal, serum-free and manufactured to regulatory standards Designed growing and feeding CHO cells in serum-free conditions Designed to work together as a complete system which allows easy scale up Allow easier filtration while maintaining cell growth and viability Provides protein titers equivalent or better compared to competitors PowerCHO and ProCHO IgG Production IgG (ng/ml) 2,500 2,000 1,500 1,000 PowerCHO Advance Productivity Compared to Competitive Media mg/ ml ProCHO 4 PowerCHO PowerCHO 2 Commercial supplier A Commercial supplier C PowerCHO 3 Commercial supplier B Commercial supplier D Peak lgg1 PowerCHO Advance PowerCHO 1 PowerCHO 2 Comp. Product 1 Comp. Product 2 Comp. Product 3 Comp. Product 4 Comp. Product 7 Comp. Product 5 Comp. Product 6 - PowerCHO 1, 2, 3 CD Media n n n Suspension CHO ProCHO 4, 5 Media Suspension CHO PowerCHO Advance Medium n n n Suspension CHO ProCHO AT Medium n n Adherent CHO Ordering inmation PowerCHO 1 CD Medium 1 L PowerCHO 2 CD Medium 1 L PowerCHO 3 CD Medium 1 L ProCHO 4 Medium 1 L ProCHO 5 Medium 1 L PowerCHO Advance Medium 1 L ProCHO AT Medium 1 L 5

6 BioResearch BioWhittaker Specialty Media Renal Media Pro293 Defined Media Formulas both suspension and adherent culture ProVero 1 Medium Supports Vero and MDCK in viral vaccine applications UltraMDCK Defined Medium Supports MDCK in viral vaccine applications at both high and low densities ProMDCK Medium Serum free media that supports the expansion and virus infection of MDCK cells in planar culture and on microcarriers Pro293 s Easy Adaptation to Suspension Culture Viable Cells /ml (x 10 5 ) Pro293 a Supports Adherent High-density Cell Growth Viable Cells (RFU) 60,000 50,000 40,000 30,000 20,000 10, Days Pro293 s-cdm Commercial Supplier A Pro293 a-cdm Commercial Supplier B Days Pro293 s Defined Medium n n n HEK 293 suspension Pro293 a Defined Medium n n n HEK 293 adherent ProVero 1 Medium n n Vero, MDCK ProMDCK Medium n n MDCK in 2D and 3D* UltraMDCK Defined Medium n n MDCK, Vero *ProMDCK 3D available exclusively through Sartorius Stedim Pro293 s Defined Medium 1 L Pro293 a Defined Medium 1 L ProVero 1 Medium 1 L ProMDCK 2D Medium 1 L UltraMDCK Defined Medium 1 L 6

7 Insect Medium Insect-XPRESS Insect Cell Medium Versatile medium shaker flask or stationary culture of SF9, SF21, High Five and Drosophila cells Insect-XPRESS Medium SF9, SF21, High Five and Drosophila cells Ordering inmation Insect-XPRESS Medium 500 ml Insect-XPRESS Medium 1 L Hybridoma Media UltraDOMA Defined, Serum-free Hybridoma Medium Designed cultivation of murine, human and chimeric hybridomas in batch culture ProDoma Serum-free Hybridoma Medium, protein-free media your hybridoma needs Scalable from small flasks to large-scale bioreactors UltraDOMA -PF Defined, Hybridoma Medium Based on the al hybridoma media murine, human, and chimeric cells in a completely medium with no peptides or tissue extracts UltraDOMA Defined Medium n n Murine, human, and chimeric cell lines UltraDOMA -PF Defined Medium Murine, human, and chimeric cell lines ProDoma 1 Defined Medium n n n Murine, rat, human, and chimeric cell lines ProDoma 3 Defined Medium n n Murine, rat, human, and chimeric cell lines Ordering inmation UltraDOMA Defined Medium 500 ml UltraDOMA -PF Defined Medium 500 ml ProDoma 1 Defined Medium 1 L ProDoma 3 Defined Medium 1 L 7

8 North America Scientific Support: (toll free) Lonza Walkersville, Inc. Walkersville, MD For research use only. Not use in diagnostic procedures. High Five is a trademark of Boyce Thompson Institute Plant Research Inc. All trademarks belong to Lonza or its affiliates or to their respective third party owners. The inmation contained herein is believed to be correct and corresponds to the latest state of scientific and technical knowledge. However, no warranty is made, either expressed or implied, regarding its accuracy or the results to be obtained from the use of such inmation and no warranty is expressed or implied concerning the use of these products. The buyer assumes all risks of use and/or handling. Any user must make his own determination and satisfy himself that the products supplied by Lonza Group Ltd or its affiliates and the inmation and recommendations given by Lonza Group Ltd or its affiliates are (i) suitable intended process or purpose, (ii) in compliance with environmental, health and safety regulations, and (iii) will not infringe any third party s intellectual property rights Lonza. All rights reserved. CD-BR020VWR 11/16 Contact Inmation 1016 Lit. No W