Custom Antibody Services. Antibodies Designed Just for You. HuCAL Recombinant Monoclonal Antibody Generation Service

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1 Custom Antibody Services Antibodies Designed Just for You HuCAL Recombinant Monoclonal Antibody Generation Service

2 Antibodies Designed Just for You What if there was a technology that would allow you to carry out research and development that was previously impossible? BIO-RAD S CUSTOM RECOMBINANT ANTIBODY SERVICE OFFERS: Selection from an established synthetic library of 45 billion fully human antibodies High specificity achieved using in vitro guided selection strategies Success rate of 90%, including challenging specificities, such as low immunogenic or toxic antigens Purified antibodies dispatched in as little as 8 weeks Fab and full length immunoglobulin format options for assay design flexibility Antibody sequence available High quality and reproducibility through consistent recombinant production methods immunization of animals, reducing the use of animals in research Proven experience as a result of generating more than 35,000 antibodies Whether you are trying to characterize a novel protein or design a new assay for a biomarker or unique biological product, the specialized antibodies you need often just don't exist. This is where Bio-Rad s antibody generation experts come in. We can make your ideal antibody a reality, enabling you to do the outstanding research that leads to new discoveries, and the excitement and rewards that brings. Our highly experienced technical specialists work with you to design the blueprint for the specialized antibodies you need. A recombinant monoclonal antibody made using HuCAL technology ensures you get the exact antibody - highly specific and high quality - that works in your hands and with clear advantages over antibodies generated using traditional methods. What more could you achieve with custom antibodies designed just for you? Let the antibody experts at Bio-Rad design and generate monoclonal antibodies to meet your precise requirements. Custom Antibody Our custom recombinant monoclonal antibody generation service utilizes the well-established and powerful Human Combinatorial Antibody Library (HuCAL ) and CysDisplay, a proprietary method of phage display, combined with automated, high-throughput processes developed by scientists at Bio-Rad. Custom Antibody

3 We transform your ideas into your unique antibody blueprint FAB SERVICES Conversion of Fab format [Approx. 5 weeks] S -S- Library of 45 billion antibodies HuCAL PLATINUM - STANDARD OPTIONS GUIDED SELECTION DESIGN YOUR SPECIFICITY Epitope specific Modification specific: phosphorylated, oxidized Shared epitope, different proteins Sandwich ELISA pair Anti-idiotypic, types 1, 2 and 3 Special conditions: denatured, buffer, temperature Select for or exclude cross-reactivity Recognize/differentiate homologs Rapid Pool Maturation (RapMAT ) PRIMARY SCREENING Bead-based Flow cytometry Inhibition assay [Add 1-2 weeks] SECONDARY SCREENING Off-rate screening, binding strength Western blotting [Add 2-4 weeks] PURCHASE SEQUENCE Possible at any time CHARACTERIZATION & MODIFICATION PK, ADA bridging assay Inhibition assay Epitope binning Affinity measurement Extended QC for critical reagents Endotoxin reduction Conjugation Fab production [From 2 weeks] Purchase sequence or FabXpress Kit IG SERVICES Convert to full length antibody Human IgGs, IgA, IgE, IgM Chimeric mouse, rat [Approx. 10 weeks] Purchase sequence Standard service generates Fab antibodies in 8 weeks 3 weeks 1 week 1 week 3 weeks PHAGE DISPLAY 3 ROUNDS SUBCLONING COLONY PICKING SCREENING SEQUENCING EXPRESSION & PURIFICATION QUALITY CONTROL DISPATCH ANTIBODY PRODUCTION STANDARD OPTIONS ANTIGEN Protein Peptide Antibody Hapten Whole cells DNA, oligo Toxin Carbohydrate Inactive virus/bacteria ANTIGEN GENERATION SERVICES Bacterial expression Mammalian expression Peptide design, synthesis Purchase commercial product [Add 4-12 weeks] 1,000-10,000 Plate with E. coli colonies producing Fab 370 clones Master plate hits Identification of positive clones by ELISA ~20 clones Identification of unique antibodies DATABASE All unique Fabs Fab available in different formats and with different tags Specificity (ELISA) Purity (SDS PAGE) APPLICATION TESTING ELISA pair selection (plate/bead-based) Flow cytometry Western blotting MATURATION SERVICES Affinity measurement Single binder maturation Cross cloning [Project starts within 3 weeks of antigen being received]

4 The Key to Your Design Blueprint Say Goodbye to Traditional Methods Make Your Ideas a Reality Guided Selection Strategies Drawing on many years of experience, we design guided selection strategies to select highly specific antibodies from the antibody library such as epitope and phospho-specific antibodies, anti-idiotypic antibodies, and sandwich ELISA pairs. SOME EXAMPLES TO GET YOU THINKING Anti-biotherapeutic antibodies Inhibitory n-inhibitory Drug-target-complex specific Differentiate between closely related proteins Modified epitopes, such as phosphorylation, oxidation, methylation Neo-epitopes created by protein cleavage Targeted epitopes, like an enzyme active site Homologous proteins Conformational changes Serotypes of a virus Antibodies with cross-reactivity between closely related proteins Antibody phage library GUIDED SELECTION WITH BLOCKING A closely related blocking antigen is used to remove crossreactive antibodies (counter selection). Guided selection strategies involve blocking steps or the use of two or more antigens, and are typically used for the isolation of epitopespecific antibodies or for antibodies that recognize shared epitopes on different antigens. Antigen In addition to the superior specificities provided by guided selection strategies, there are numerous other advantages of HuCAL technology compared to conventional monoclonal antibody generation. Parameter HuCAL Monoclonal Conventional Monoclonal Technology Antibody specificity Library of 45 billion antibodies Phage display Bacterial and mammalian expression Designed just for you using guided selection Immunization Success rate ~ 90% ~ 75% Complex cell culture Speed of delivery 8 weeks 4-9 months Flexible formats Fab antibodies Full length Ig, many isotypes Antibody sequence Sequence available Unknown Fully human Yes, with choice of human, mouse or rat Fc Driven by immune system, less control Extensive screening required We work on a wide variety of antibody generation projects and each one is unique. CHOOSE THE NEXT STEPS THAT WORK BEST FOR YOU LEARN MORE ONLINE Watch our video Designed just for you to see inside the custom antibody facility and discover more about the technology behind Bio-Rad s custom recombinant antibodies. Find additional resources explaining the antibody generation process in detail, with example case studies, publications and technical articles. REQUEST A TECHNICAL PRESENTATION One of our antibody specialists can present the HuCAL technology to you, answering your questions and addressing your specific requirements. DISCUSS YOUR PROJECT IDEAS Once your ideas start to take shape, your specialist will work with you to design a detailed blueprint for your antibody. You may also wish to visit the HuCAL technology facility in Germany to meet our scientists and see how the process works. WORKING WITH US Your project is managed by a dedicated team, passionate about making antibodies, and experts at what they do. We aim to provide antibodies designed for you that work for you, and an experience that exceeds your expectations. OUR CUSTOMERS INCLUDE: Large pharmaceutical and small biotech companies In vitro diagnostic and research assay manufacturers Cosmetic, food and environmental companies Academic and government research institutes Closely related blocking antigen Antibody engineering and optimization Choice of detection tags Affinity maturation Animals used Yes Phage displaying Fab antibody BIND WASH ELUTE

5 Visit bio-rad-antibodies.com/blueprint to find out more. HuCAL generated antibodies are not intended for use in humans, and may not be used for therapeutic, prophylactic or palliative purposes. Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. HuCAL, HuCAL PLATINUM, CysDisplay and RapMAT are trademarks of MorphoSys AG. Bio-Rad Laboratories, Inc. Life Science Group Web site bio-rad-antibodies.com Austria, Germany, Netherlands & Switzerland Belgium & Luxembourg Canada & USA France United Kingdom, Scandinavia & other countries Custom Services Bulletin 7107 Ver A US/EG 05/18 Sig 0118