Featuring Analyst software under Windows NT for enhanced performance and ease of use. API 150EX. LC/MS System

Transcription

1 Featuring Analyst software under Windows NT for enhanced performance and ease of use. API 15EX LC/MS System

2 compact, rugged, The API 15EX LC/MS System is the most rugged and reliable single quadrupole LC/MS system available. It delivers superior performance for your most demanding applications characterization of compounds in lead discovery and optimization, identification of pharmaceutical impurities and degradation products, quantitative and qualitative studies for drug discovery, and analysis of proteins and peptides cm x 63.5 cm footprint (not including computer) A Complete Design The API 15EX system was designed by scientists who are pioneers in LC/MS experts at Applied Biosystems/ MDS SCIEX to service the most demanding laboratories. The gold-plated ceramic quadrupole mass analyzer minimizes system temperature dependence. Your API 15EX system offers highly stable mass calibration and does not require constant tuning. Complete Dual 5LPS turbo pumps are aircooled, low-maintenance systems that provide rapid pumpdown and automatic maintenance of the analyzer vacuum. This space-efficient system fits on most laboratory benches. Rugged Dependable nitrogen Curtain Gas technology from Applied Biosystems/ MDS SCIEX keeps your system running dependably by protecting the ion path from contamination, especially with samples from biological fluids. Reliable Analyst software for Windows NT provides powerful system control and data manipulation capabilities. Highly efficient ion optics provide excellent transmission for optimal sensitivity. Dependable, high-performance TurboIonSpray and Heated Nebulizer (APCI) sources achieve superior results over a wide range of sample types, solvents, and flow rates.

3 reliable Nitrogen Curtain Gas Interface Mechanical Pump Skimmer QUADRUPOLE TECHNOLOGY CEM Detector 1 Nitrogen Curtain Gas interface technology protects the ion pathway from contamination. 2 Patented high-pressure collisional focusing system (Q) concentrates analyte ions into a focused isoenergetic beam for efficient mass filtering in the analyzer section. Orifice Q Turbomolecular Pump Prefilter Q1 Turbomolecular Pump 3 Gold-plated ceramic quadrupole rods (Q1) provide extremely stable mass calibration, so the system does not require constant tuning. That means the API 15EX system is much more reliable than many other systems on the market. 4 Digital pulse-counting detector provides very high sensitivity and does not respond disproportionately to ion clusters. Curtain Plate *US Patent Nos. 4,963,736, 5,179,278, 4,328,42 & 5,248,875 SPRAYING AT ORIFICE Nitrogen Curtain Gas Interface Differentially Pumped Interface Orifice Plate API Ion Source Vacuum Chamber Curtain Gas Flow Skimmer Intensity (million cps) Away from orifice Exclusive nitrogen Curtain Gas interface system from Applied Biosystems/MDS SCIEX protects the ion path for maximum ruggedness and resistance to contaminants. 8 Away from orifice 4 Horizontal Sprayer Position Axial Sprayer Position Optimized API interface gives maximum response with minimal adjustment. A broad area of ion acceptance and easy source optimization simplifies method development and maximizes productivity.

4 power and advanced technology for powerful data processing With Analyst software, the API 15EX system is easier to use than ever before. Now, the power of mass spectrometry is available to all LC/MS users, novice and expert.the superior selectivity and specificity of MS detection accelerates assay development and decreases analysis time. Analyst software is a PC-based Windows NT package for Applied Biosystems/ MDS SCIEX LC/MS systems. It is designed to provide full control of data acquisition, integrated control of LC peripherals, and powerful data manipulation capabilities. While the API 15EX system can rapidly analyze some compounds using flow injection analysis (FIA), it can interface with various HPLC systems to analyze complex mixtures. Analyses can also be performed using collision induced dissociation (CID) to produce characteristic fragment ions revealing the chemical structure of molecules. The system can also obtain molecular weight and structural information in a single analysis, using powerful mixed mode analyses saving time and conserving valuable samples. Laboratory analysts will appreciate how quickly and accurately the API 15EX system screens for expected molecular weight over a wide analyte concentration range. Routine analysis and method development time is reduced, allowing for increased productivity. The API 15EX system is a rugged and reliable system that offers unrivaled sensitivity and quantitative linearity.this easy to use system accommodates a wide variety of quantitative assays. Intensity, cps Intensity, cps Intensity, cps 6e6 4e6 2e6 e 5.e4. High- and low-energy spectra of the degradant at RT=4.66 min vs. those of the Photolyte 1 standard Degraded sample, RT=4.66 DP= e5 Photolyte standard DP=2 4e5 2e Time (minutes) Intensity, cps Intensity, cps e5 3e5 2e5 1e5 Degraded sample, RT=4.66 DP= Photolyte 1 standard DP= Intensity, cps E Using CID, the fragmentation patterns from peptides can yield direct information leading to knowledge of their amino acid sequences. N E Glutamic Acid N Asparagine V Valine I Isoleucine V I Reproducible CID was able to identify a Lomefloxacin (antibacterial) degradant.

5 sensitivity robust data, unparalleled sensitivity The API 15EX system delivers essential molecular weight and structural information to produce the right answers faster. Delivering superior throughput, rugged and reliable performance, and a marked improvement in your productivity, the API 15EX system is one of the most advanced, easy to use systems of its kind. The system consistently performs well, even with samples from physiological fluids, and provides excellent linearity with high sensitivity. 7.e+6 Peak Area (counts) Injection: Haloperidol in plasma (proteins precipitated) No. of Injections: 13 injections over 1 hr CV: 2.8% Relative Response (%) 5 2: pg injected 5 1 Time (hours) Ruggedness: Analysis of haloperidol, an antidyskinetic, from protein precipitated plasma by LC/MS single-ion monitoring (SIM). 1: 2: 3: 4: 5: Sensitivity: LC/MS analysis of 2.4 pg of an antihistamine from protein precipitated plasma. 1.2e7 Peak Area (counts) 6e Nanograms Injected Quantitative Linearity: Analysis of an antihistamine (2.4 pg to 2 ng) from protein precipitated plasma (correlation coefficient=1.).

6 control powerful systems control The API 15EX system with Analyst software affords you integrated system control over a variety of LC pumps and autosampler modules. Analyst software helps make your LC/MS tasks easier so that you can obtain the data you want in the formats you need. Easy resolution optimization uses pre-defined routines to automatically set peak width and mass calibration. Quantitative optimization automatically determines instrument settings for the best SIM signal for accurate quantitative analysis of a compound. The powerful quantitative capability of Analyst software allows rapid processing of quantitative data and automatic extraction of critical information from data sets through queries and metric plots. The system s pump and autosampler are fully integrated and controlled as part of the method. Available LC devices are conveniently set up in the hardware configurator. Regardless of the brand of LC module controlled, Analyst software provides the same easy to use control interface. Quantitative results: Analyst software offers you an innovative set of advanced tools for enhanced quantitative data processing. You can view tables, graphs, and peak data all on one screen. Metric plot: Two mouse clicks were all it took to generate this metric plot of IS Peak Area vs. Injection Number. Monitoring the IS Peak Area is also an indicator of the system s stability. Auto optimization: Enter basic compound information, and Analyst software automatically optimizes data by locating the correct mass and preparing a method for quantitation. This figure is an example of how auto optimization adjusts ion path parameters in order to maximize signal.

7 support At Applied Biosystems/MDS SCIEX, we are committed to providing innovative, technologically advanced developments at the best possible value. We provide a comprehensive instrument warranty and offer maintenance plans to suit your needs and budget. You can count on us to support your API 15EX system with the following: Expert consultation and technical support to ensure maximum system performance and productivity. Online support via the Web at On-site method development and continued application support. Training classes covering basic operation, preventive maintenance, and advanced techniques and applications. CD-ROM manuals to browse or copyand-paste to create your own standard operating procedures. Validation support for regulatory agency compliance.

8 from the pioneers in LC/MS Applied Biosystems/MDS SCIEX introduced the first dedicated LC/MS/MS system based on innovative IonSpray technology (pneumatically assisted electrospray ionization) in Today, Applied Biosystems/MDS SCIEX maintains the largest installed base of dedicated LC/MS and LC/MS/MS systems in the world. Our LC/MS systems have revolutionized mass spectrometry by dramatically increasing the range of compounds that can be analyzed. The API 15EX system offers easy to use, sophisticated Analyst software for controlling all functions of your mass spectrometer. Now you can completely automate data acquisition and analysis, verify performance, and initiate data processing. To find out more about our powerful LC/MS systems, call or visit The API 15EX system is part of a comprehensive line of superior technology solutions for LC/MS and LC/MS/MS. API QSTAR Pulsar Hybrid LC/MS/MS System Worldwide Sales Offices Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches into 15 countries on six continents. For international office locations, please call the division headquarters or refer to our Web site at API 3 LC/MS/MS System Applera Corporation is committed to providing the world s leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses. Applied Biosystems/MDS SCIEX is a joint venture between Applera Corporation and MDS Inc. Headquarters 85 Lincoln Centre Drive Foster City, CA 9444 USA Phone: Toll Free: Fax: API 2 LC/MS/MS System For Research Use Only. Not for use in diagnostic procedures. AB (Design), Analyst, API 15EX, API 2, API 3, Curtain Gas, QSTAR, and TurbolonSpray are trademarks, and Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and certain other countries. MDS and SCIEX are trademarks of MDS Inc. Windows NT is a registered trademark of Microsoft Corporation. 21 Applied Biosystems. All rights reserved. Printed in the USA, 3/21, PJA Publication 114BR6-1