18. Durability studies carried out in the present investigation in. ordinary grade and standard concrete, through acid attack test

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1 18. Durability studies carried out in the present investigation in ordinary grade and standard, through acid attack test with cubes immersed in 5% H2SO4 and 5% HCL indicates that bacterial is more durable in terms of Acid Durability Factors than conventional and also bacterial is less attacked in terms of Acid Attack Factors than conventional. 19. The Charge Deterioration Factors in ordinary grade and standard for a given effective cover, at a given time for bacterial are lowest than that of reference. CONTENTS TOPIC CHAPTER 1: INTRODUCTION 1.1 History of Microbiology 1.2 Classification of bacteria 1.3 Various bacteria used in 1.4 Bacillus subtilis JC3 1.5 Polyphasic characterization of strain JC3 1.6 Maintenance of stock cultures 1.7 Bacterial PAGE No CHAPTER II: REVIEW OF LITERATURE 10

2 2.1 Microorganisms Bacillus subtilis 2.2 Bacterial 2.3 Strength Studies Compressive strength and split tensile strength of Stress-Strain behaviour of Flexural behaviour of beams 2.4 Durability Studies CHAPTER III: SCOPE AND OBJECTIVES OF PRESENT INVESTIGATION 3.1 Objective of the present investigation Growth of bacteria Bacillus subtilis JC To study the Strength of Cement Mortar To study the Compressive Strength and Split Tensile Strength of To study the Stress-Strain behaviour of To study the Flexural behaviour of To study the Strength loss, Weight loss, Acid Durability Factor and Acid Attack Factor of To study the Accelerated Corrosion of Cracking in 35 CHAPTER IV: EXPERIMENTAL INVESTIGATION 4.1 Experimental programme 4.2 Materials used and their properties

3 4.2.1 Cement Ennore Sand Coarse aggregate Fine aggregate Water Bacteria 4.3 Mix Design Ordinary grade (M20) Standard grade (M40) 4.4 Mixing of 4.5 Phase I Growth of bacteria - Bacillus subtilis JC Dendogram 4.6 Phase II To study the Compressive Strength of Cement Mortar Specimen preparation Testing procedure To study the Compressive Strength and Split Tensile Strength of Specimen preparation Testing procedure To study the Stress- Strain behaviour of Specimen preparation Testing procedure

4 4.6.4 To study the Flexural behaviour of Specimen moulds preparation Fabrication process Casting and curing Preparation of specimen for testing Testing procedure Measurement of curvature Measurement of deflections 4.7 Phase III To study the Strength loss, Weight loss, Acid Durability Factor and Acid Attack Factor of Specimen preparation Testing procedure To study the Accelerated Corrosion of Cracking in Specimen preparation Testing procedure CHAPTER V: RESULTS OF THE EXPERIMENTAL INVESTIGATION 5.1 Results of the general observations Physical properties of ordinary Portland cement Properties of aggregate Sieve analysis of fine aggregate Sieve analysis of coarse aggregate 54

5 Properties of fine and coarse aggregate 5.2 Phase-I : Culture of Bacteria Dendrogram 5.3 Phase-II : Strength Studies To study the compressive strength of cement mortar cubes To study the compressive strength and split tensile 56 strength of To study the stress-strain behavior of To study the flexural behaviour of 5.4 Phase-III : Durability Studies To Study the strength loss, weight loss, Acid Durability Factor and Acid Attack Factor of To study the Accelerated Corrosion of cracking in 115 CHAPTER VI: ANALYSIS 6.1 Introduction to Stress-Strain behaviour 6.2 Mathematical modeling for Stress-Strain behaviour of Non-Dimensional Stress-Strain curves Models available for Stress-Strain curves of Proposed Model for Stress-Strain behavior of conventional and bacterial 6.3 Calculation of theoretical stresses using proposed 178

6 analytical equations Development of theoretical Stress-Strain curves 6.4 Theoretical computation of Moments and Curvatures Calculation of neutral axis depth Moment of Resistance Offered by the Materials Calculation of theoretical curvature 6.5 Confinement Index 6.6 Stress Block parameters CHAPTER 7: DISCUSSION OF TEST RESULTS 7.1 Culture of bacteria 7.2 Strength studies To study the strength of cement mortar To study the compressive strength and split tensile strength of Stress-strain behaviour of conventional and bacterial 190 mixes Stress-strain response of conventional and bacterial 191 mixes Mathematical model for stress-strain curves of 191 conventional and bacterial mixes Toughness of conventional and bacterial 192 mixes Flexural behaviour of conventional and bacterial 193 beams

7 Moment curvature relationship Theoretical moment-curvature values Comparison of experimental and theoretical momentcurvature values and validation Strength loss, weight loss, acid durability factor and 197 acid attack factor of Relative charge, total charge and deterioration factors 198 obtained from accelerated corrosion test CHAPTER 8: CONCLUSIONS 8.1 CULTURE OF BACTERIA 8.2 STRENGTH STUDIES Studies on compressive strength of cement mortar cubes Studies on compressive strength and split tensile 202 strength of Studies on stress-strain behaviour of Studies on flexural behavior of 8.3 DURABILITY STUDIES Studies on strength loss, weight loss, acid durability factor and acid attack factor of Studies on accelerated corrosion of cracking in Scope for further study 208

8 APPINDIX PLATES (PHOTOGRAPHS) BIBLOGRAPHY LIST OF PUBLICATIONS LIST OF TABLES Table Title Page No. No Physical properties of ordinary Portland cement Properties of aggregate Sieve analysis of fine aggregate Sieve analysis of coarse aggregate Properties of fine and coarse aggregate Dendrogram 55