Product Overview April 2014

Transcription

1 Product Overview April 2014

2 About BioCision The lack of standardized, high-quality biospecimens has been widely recognized as one of the most significant roadblocks to the progress of cancer research. - NIH / NCI / BBRB Develop products to address unmet standardization needs in preanalytical sample handling, processing, storage and transport BioCision products improve pre-analytical standardization of temperature-sensitive bio-medical samples: Day to day User to user Lab to lab Site to site Page 2

3 Consistent. Reproducible. Standardized. Sample Temperature Management CoolRack and CoolBox for reproducible preparation of temperature-sensitive samples Cell Cryopreservation CoolCell products for standardized, controlled-rate cell freezing Intuitive design. Proprietary advanced thermal management technology. Page 3

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5 Current methods are inconsistent and non-reproducible mmm Cooling samples in ice or gel-filled cooling blocks introduces a variety of problems. Ice cooling Variable sample temp Contamination Wet, illegible labels Non-reproducible method Gel-filled coolers Highly variable sample temp (edge effect) Short cooling duration Cannot use while re-charging Page 5

6 CoolProducts provide consistent and reproducible sample cooling and freezing Using a thermo-conductive CoolRack module as an interface between the ice and sample provides a consistent and reproducible cooling method. Eliminating ice with a CoolBox system further minimizes the risk of sample contamination and provides hours of consistent and reproducible ice-free cooling or freezing. Page 6

7 CoolRack modules ensure reproducible cooling and thawing profiles Performance test: A temperature probe was placed into a 2.0 ml cryogenic vial containing 1.0 ml of water. The tube was inserted into a CoolRack CF45 thermo-conductive module. The module was placed onto a ThermalTray platform in a 37 C water bath and allowed to equilibrate. The CoolRack CF45 module was then removed and placed onto dry ice and equilibrated to -78 C (0-15 minutes) and then returned to the water bath to re-equilibrate to 37 C (15-30 minutes). This experiment was repeated five consecutive times and temperature profiles were recorded. Conclusion: The CoolRack CF45 module showed identical cooling profiles and phase transition (orange circles) over five consecutive freeze-thaw cycles. Page 7

8 CoolRack, CoolSink, ThermalTray: Compatible with broad temperature range -150 C -78 C 0 to 4 C 37 C + Liquid Nitrogen Dry Ice Ice Water Bath Adapts from ambient (25 o C) to approx o C in minutes Samples are upright and organized as they flash freeze No direct contact between sample and LN 2 Reproducible method Adapts from ambient (25 o C) to -78 o C in 5-7 minutes No need for ethanol cost savings, no hazardous waste, no sunk tubes Samples are upright and organized as they snap-freeze Same freezing rate as direct immersion into dry ice or alcohol slurry Reproducible method Adapts from ambient (25 ç C) to <4 ç C in seconds Samples stay dry, organized, uniform in temperature Labels do not get wet or illegible All samples +/- 0.1 o C Reproducible method Adapts to 37 C in approx. 10 minutes Using a ThermalTray platform and a CoolRack tube module, samples stay above water line minimizing risk of contamination Samples remain dry and organized Uniform sample temperature Reproducible method Use for very cold or very hot applications (LN 2 to >100 C). Samples protected from direct contact with temperature source. Page 8

9 CoolRack, CoolSink, ThermalTray: Accommodates variety of tubes and plates CoolRack tube modules Including SBS-format modules CoolSink plate modules* Including SBS-format modules ThermalTray platforms* For supporting CoolRack and CoolSink modules in liquid * Patented Page 9

10 CoolBox : Ice-free workstations CoolBox XT & 2XT CoolBox MP CoolBox 30 XT Starter Holds Tubes Holds Plates Page 10

11 CoolBox: How it works CoolBox XT and 2XT CoolBox MP and 30 Lid CoolRack or CoolSink sample module Cooling Cartridge (Pre-frozen in -20 C freezer) Base Simply pre-freeze the Cooling Core or cartridge in a -20 C freezer, place it in the base of the CoolBox, and rest a CoolRack or CoolSink module on top. Page 11

12 CoolBox and CoolRack: Accessories TruCool ice pans and buckets Made of non-toxic EVA foam Non-skid bottom, insulative and non-sweating 5 sizes, 7 colors TruCool microfuge tubes and cryogenic vials CryoCeps cryovial grippers Microfuge tubes feature patented pull back cap ensures thumb never passes over open top minimizing contamination risk Cryogenic vials feature unique thermally-fused gasket (replaces O-ring) and bar-codes for secure identification CryoCeps grippers protect fingers from handling frozen vials and tubes CoolProbe temperature monitors Monitor sample temperature while using CoolRack and CoolSink modules in ice Fitted probes for CoolRack M, CoolRack CF(T) Universal probe for other CoolRack and CoolSink modules Page 12

13 CoolBox and CoolRack: Use for a variety of applications If you currently use an ice bucket, gel-filled coolers, refrigerators, freezers, or water bath, CoolBox and CoolRack products will improve your processes. Ideal for: Tissue / Cell culture Tissue dissections & snap-freezing Cell culture preparation and thawing Protein / Proteomics Protein isolation, sonication, fractionation Trypsin digestion Molecular biology PCR preparations RNA isolation Reagent cooling Microbiology Bacteria and virus freezing Visit for application assistance. Page 13

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15 CoolCell : Alcohol-free cell freezing CoolCell CoolCell SV2 CoolCell FTS30 CoolCell 5ml LX CoolCell LX For a variety of tubes and capacities. The new standard for passive cell cryopreservation. Page 15

16 CoolCell: How it works Passive controlled-rate cell freezing container Replaces alcohol-based containers such as Mr. Frosty Simply fill with vials and place -80 o C freezer Radially-symmetric thermal exchange design ensures uniform cooling profiles to all vial positions Composed of high-density materials used in aerospace industry for lightweight & insulative properties Highly thermo-conductive alloy core Models include conduction and/or micro-convection air flow control Equal or better results than programmable step-down freezers and alcohol-based freezing containers Patent Pending Replaces Mr. Frosty and programmable freezers. No alcohol. No on-going maintenance or cost. No hazardous waste disposal. Mr. Frosty is a trademark of Thermo Fisher Scientific. Page 16

17 CoolCell containers ensure reproducible freezing profiles Five consecutive freezing runs show extremely consistent thermal profiles. Page 17

18 CoolCell vs. Mr. Frosty CoolCell Isopropanol Container Consistent freezing rate All vials have uniform freezing profiles No isopropanol No maintenance No pre-cooling No hazardous waste No hidden cost Once you buy it, you re done paying for it!! Easy to use Cap removes easily when frozen Not cold to the touch when frozen, no gloves needed Ready for reuse after 5 minute warm-up time Minimal impact on freezer 1/3 the heat impact on freezer; nearby samples remain unharmed Inconsistent freezing rate Isopropanol degradation ensures variability Two circles of vials results in further non-uniform heat removal Requires Isopropanol Track uses so can replenish IPA every 5 th use On-going maintenance Spill risk Pre-cool in refrigerator (safety issue) > $350 per unit per year to maintain properly Isopropanol expense Hazardous waste disposal costs Difficult to handle Cap is difficult to open when frozen, unit is slippery (cells are warming up) Protective glove required when handling frozen unit Unit is cold, slippery, breakable Impacts local freezer area Large heat capacity removed from alcohol impacts nearby samples Page 18

19 CoolCell: Compatible with many cell types Cell Lines LnCap, HTB77, A549, others Data and testing done by Lonza (Germany) Primary Cells Variety of primary cells tested by labs at UCSF and ZenBio Stem Cells and Cell Therapy Products Testing and data performed by USCF (Bluestone lab), TxCell and others See CoolCell data at: Viability and post-thaw functionality as good or better than Mr. Frosty and programmable freezers. Page 19

20 Cell Cryopreservation: Accessories XT Starter ice-free cooling platform Ideal for use in the hood where ice is forbidden XT Starter and CoolRack modules provide compact, icefree cooling for cryogenic and cell therapy vials TruCool Hinged CryoBoxes Patented hinged lid ensures lid is always put on correctly, keeping markings in synch with cryovials Boxes for 1ml & 2ml vials, and for taller vials 6 bright colors With or without LN 2 drain holes TruCool cryogenic vials CryoCeps cryogenic vial grippers Patented fused gasket layer on vial closure ensures leak-proof seal Bar-coded vials for secure sample identification CryoCeps* protect fingers when handling frozen vials * Patent pending Page 20

21 Thank you! For questions, contact us at: BioCision, LLC. All rights reserved. BioCision, Standardizing Samples, CoolRack, CoolSink, ThermalTray, CoolBox, CoolCell, TruCool, CoolProbe, CryoCeps are designated trademarks owned by BioCision, LLC..