High Performance Polymer Chromatography Products

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Crystal Stewart
5 years ago
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1 High Performance Polymer Chromatography Products

Quality, Innovation Competitive Price Welch Materials develops and manufactures chromatography

flash columns, sample vials, syringe filters, and chromatographic bulk media.

Welch Materials was established in 2003 in both Maryland, USA and Shanghai, China.

2 Quality, Innovation Competitive Price Welch Materials develops and manufactures chromatography consumables including HPLC columns, solid phase extraction (SPE) columns, GC columns, QueChERS, flash columns, sample vials, syringe filters, and chromatographic bulk media. HPLC instruments and SPE automation equipments are also in the product lines of Welch. Welch Materials was established in 2003 in both Maryland, USA and Shanghai, China. Our initial strength was our extensive experience on particle surface modification science and techniques. We are experts on bonding chemistry and innovative packing materials for chromatography applications. Through the optimal utilization of our resources across the two countries, we have developed many innovative LC and GC consumable products, in particular, our HPLC columns and SPE products. A new facility located in Zhejiang province, China will be put in use soon as the headquarter and manufacturing site of Welch Materials.

Quality, Innovation, Competitive Price Polymer Reversed Phase Chromatography Media Pore Sizes Poly(styrene-co-divynylbenzene) 3, 5, 10, 15, 20, 30, 40, 50

30µm Figure 1. Monodisperse polymer spheres with controllable particle size and pore size Mobile phase A Mobile phase B Gradient 4.6 mm I.D.

1 % TFA in ACN 20 %-40 % B for 20 min 40 % B for 10 min 1 ml/min 80 %B~95 %B 10 min UV @ 280 nm Figure 2.

poly(styrene-co-divynylbenzene) 3, 5, 10, 15 and 20 µm 5%, 8% and 10% Na +, Ca 2+, K + and Pb 2+ Carbohydrates and organic acids 5µm 10µm Figure 3.

3 Quality, Innovation, Competitive Price Polymer Reversed Phase Chromatography Media Pore Sizes Poly(styrene-co-divynylbenzene) 3, 5, 10, 15, 20, 30, 40, 50 and 60 µm 300, 500, and 800 Å Proteins, peptides, nucleic acids, antibiotics, and low molecular weight pharmaceuticals 3µm 5µm 100 Å 300 Å 500 Å 800 Å 10µm 30µm Figure 1. Monodisperse polymer spheres with controllable particle size and pore size Mobile phase A Mobile phase B Gradient 4.6 mm I.D. x 250 mm, 10 μm, Xtimate TM PS/DVB 0.1 % TFA in water 0.1 % TFA in ACN 20 %-40 % B for 20 min 40 % B for 10 min 1 ml/min 80 %B~95 %B 10 min 280 nm Figure 2. Reproducible separation of proteins with 8 batchs Carbohydrate Analysis Chromatography Media Crosslink Degree Metal Ions Chelated Sulfonating poly(styrene-co-divynylbenzene) 3, 5, 10, 15 and 20 µm 5%, 8% and 10% Na +, Ca 2+, K + and Pb 2+ Carbohydrates and organic acids 5µm 10µm Figure 3. SEM of carbohydrate analysis chromatography media. Mobile phase Temperature Injection Volμme Sample Xtimate TM Ca, 10 µm, 8%, mm Nanopure water 0.6 ml/min 85 C 10 µl ELSD 1. Melezitose, 2. Maltose, 3. Glucose, 4. Maltitol, 5. Fructose, 6. Erythritol, 7. sorbitiol. Figure 4. Analysis of Sugar mixture on carbohydrate analysis chromatography media.

Ion Exchange Chromatography Media Pore Sizes Highly crosslinked polyacrylate microspheres 10, 15, 20, 30, 40 and 50 µm 300, 500, and 800 Å Proteins, oligonucleotides, peptides, oligosaccharides Fig.5. SEM of ion exchange chromatography media Figure 6.

0 1 ml/min AKTA Purifier (UV@280nm) Affinity Chromatography Media Pore Size Functional Group Highly crosslinked polyacrylate microspheres 30 and 50 µm 800 Å Hydrophilic layer coated and Ni 2+

4 Ion Exchange Chromatography Media Pore Sizes Highly crosslinked polyacrylate microspheres 10, 15, 20, 30, 40 and 50 µm 300, 500, and 800 Å Proteins, oligonucleotides, peptides, oligosaccharides Fig.5. SEM of ion exchange chromatography media Figure 6. Application of ion exchange chromatography media. Sample Eluent 1 ml, Xtimate TM Q 10 ml, 5 mg/ml BSA 20 mm Tris, 1M NaCl, ph8.0 1 ml/min AKTA Purifier (UV@280nm) Affinity Chromatography Media Pore Size Functional Group Highly crosslinked polyacrylate microspheres 30 and 50 µm 800 Å Hydrophilic layer coated and Ni 2+ chelated Histidine labeled proteins Figure 7. SEM of affinity chromatography media Sample Eluent 1 ml, Xtimate TM Ni-IDA, 50 μm 10 ml 5mg/ml BSA 20 mm Tris, 1 M NaCl ph8.0 1 ml/min AKTA Purifier (UV@280 nm) Figure 8. Application of affinity chromatography media

5 Ordering Information Polymer Reversed PhaseChromatography Media Xtimate TM PS/DVB Non-porous 300 Å 500 Å 800 Å Non-porous 300 Å 500 Å 800 Å 3 μm μm μm μm μm μm μm μm μm um Carbohydrate Analysis Chromatography Media Phases 5% 8% 10% Phases 5% 8% 10% 5 μm μm Xtimate TM H (PS/DVB-SO 3 H) Xtimate TM Na (PS/DVB-SO 3 Na) Xtimate TM Ca (PS/DVB-SO 3 Ca) 10 μm 15 μm Xtimate TM K (PS/DVB-SO 3 K) 10 μm 15 μm μm μm μm μm μm Xtimate TM Pb 10 μm μm (PS/DVB-SO 3 Pb) 15 μm μm μm μm Xtiamte TM Ca 15 μm μm (PS/DVB-SO 3 Ca) 20 μm Ion Exchange Chromatography Media Phases 300 Å 500 Å 800 Å Phases 300 Å 500 Å 800 Å Xtimate TM SP (Polyacrylate -SO 3 - ) Xtimate TM CM (Polyacrylate- CH 2 COO- ) 10 μm μm μm μm μm Xtimate TM Q 20 μm (Polyacrylate- 30 μm N+(CH 3 ) 3 ) 30 μm μm μm μm μm μm μm μm μm μm Xtimate TM DEAE 20 μm (Polyacrylate- 30 μm N+H(CH 2 CH 3 ) 2 ) 30 μm μm μm μm μm Phases 800 Å Phases 800 Å Xtimate TM Ni-IDA (Polyacrylate-Ni 2 +) 30 μm Xtimate TM Ni-NTA 30 μm μm (Polyacrylate-Ni 2 +) 50 μm

6 Quality, Innovation, Competitive Price HPLC columns are listed in database Headquarter and Manufacturing Site Welch Materials (Zhejiang), Inc. 6 th floor, No.855 Xianyuan RD, Jinhua Zhejiang Province , China Tel: (86) Fax: (86) China Domestic Marketing and Sales Center Welch Materials (Shanghai), Inc. Unit B102, 326 Edison RD, Shanghai, China Tel: (86) Fax: (86) North America Sales Center Welch Materials, Inc Bluegrass RD, Photomac, MD Tel:(410) Fax:(410) India Sales Centre Imptech Scientific. 203 Veena Appartments, Dharam Karam Road Ameerpet, Hyderabad A.P., INDIA Tel: (91) Fax: (91) sales@impscience.com sales@welchmat.com Website: