MATERIAL SAFETY DATA SHEET

Transcription

1 MATERIAL SAFETY DATA SHEET 1. IDENTIFICATION OF THE MATERIAL AND SUPPLIER PRODUCT NAME: PRODUCTNUMBER : SUPPLIER: APPLIED BIOSYSTEMS 850 LINCOLN CENTRE DRIVE FOSTER CITY, CA, USA IN CASE OF EMERGENCY CALL CHEMTREC FAST SYBR GREEN PCR MASTER MIX PRODUCT USE DESCRIPTION: FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES 2. HAZARDS IDENTIFICATION Emergency Overview Warning! Combustible liquid and vapor. Irritating to eyes, respiratory system and skin. This product contains sodium azide at a concentration that is considered not hazardous according to OSHA regulations. Sodium azide may react with lead and copper plumbing to form highly explosive metal azides. Potential health effects Inhalation : May be harmful if inhaled. May cause irritation of respiratory tract. Skin : Causes skin irritation. May be harmful if absorbed through skin. Eyes : Causes eye irritation. Ingestion : May be harmful if swallowed. Page 1 of 7 Revision Date:

2 Physical and chemical hazards : Combustible liquid and vapor. This product contains sodium azide at a concentration that is considered not hazardous according to OSHA regulations. Sodium azide may react with lead and copper plumbing to form highly explosive metal azides. Chronic exposure: Kidney injury may occur. Aggravated Medical Condition: Skin disorders Kidney disorders Respiratory disorder Eyes Target Organs : Kidney 3. COMPOSITION/INFORMATION ON INGREDIENTS Hazardous components CAS-No. Weight % Glycerol % Hydroxylated Organoamine Proprietary 1-10 % Dimethyl sulfoxide % 4. FIRST AID MEASURES Eye contact Inhalation Skin contact Ingestion Immediately flush eyes for at least 15 minutes. Get medical attention. Remove to fresh air immediately. Get medical attention immediately. If not breathing, give artificial respiration. Rinse immediately with plenty of water and seek medical advice. Remove and wash contaminated clothing before re-use. If swallowed, call a poison control centre or doctor immediately. Never give anything by mouth to an unconscious person. 5. FIRE-FIGHTING MEASURES Suitable extinguishing media Water spray Carbon dioxide (CO2) Foam Dry chemical Hazardous decomposition products Special protective Carbon oxides, nitrogen oxides (NOx), Hydrogen chloride gas, Sulphur oxides Wear self contained breathing apparatus for fire fighting if Page 2 of 7 Revision Date:

3 equipment for fire-fighters necessary. Use personal protective equipment. 6. ACCIDENTAL RELEASE MEASURES Personal precautions Ensure adequate ventilation. Avoid contact with skin, eyes and clothing. Avoid breathing dust/fume/gas/mist/vapours/spray. Environmental precautions Should not be released into the environment. Prevent product from entering drains. Methods for cleaning up Clean contaminated floors and objects thoroughly while observing environmental regulations. Ensure adequate ventilation. 7. HANDLING AND STORAGE Precautions for safe handling Use only in area provided with appropriate exhaust ventilation. Wear personal protective equipment. Take precautionary measures against static discharges. Storage conditions Protect against physical damage. Isolate from incompatible substances. Store in original container. See product label for specific storage requirements. 8. EXPOSURE CONTROLS/PERSONAL PROTECTION Exposure limit(s) Hazardous components Regulation Type of listing Value Glycerol California PELtotal dust 10 mg/m3 California PELrespirable fraction 5 mg/m3 OSHA TWA 15 mg/m3 total OSHA TWArespirable fraction 5 mg/m3 ACGIH TWAMist 10 mg/m3 Eye protection Chemical resistant goggles must be worn. Ensure that eyewash stations and safety showers are close to the workstation location. Hand protection Respiratory protection Skin protection Engineering Wear suitable gloves. Please observe the instructions regarding permeability and breakthrough time which are provided by the supplier of the gloves. Also take into consideration the specific local conditions under which the product is used, such as the danger of cuts, abrasion, and the contact time. Avoid breathing vapors, mist or gas. When workers are facing concentrations above the exposure limit they must use appropriate certified respirators. For unknown concentrations or Immediately Dangerous to Life or Health (IDLH), use a full-facepiece positive pressure air supplied respirator. Wear suitable protective clothing. Maintain air concentrations below occupational exposure standards. Use Page 3 of 7 Revision Date:

4 measures only in area provided with appropriate exhaust ventilation. 9. PHYSICAL AND CHEMICAL PROPERTIES Physical state liquid Colour Odour Odour Odour Threshold ph 8.0 Boiling point Melting point Vapour pressure Water solubility Flash point Explosion limits Density Percent volatile Volatile organic compounds (VOC) content 10. STABILITY AND REACTIVITY Conditions to avoid Keep away from open flames, hot surfaces and sources of ignition. Materials to avoid Hazardous decomposition products Strong acids, Reducing agents, Acid chlorides, Oxidizing agents, Bases, Acetic anhydride, Isocyanates, Ammonia, This product contains sodium azide at a concentration that is considered not hazardous according to OSHA regulations., Sodium azide may react with lead and copper plumbing to form highly explosive metal azides. Carbon oxides, nitrogen oxides (NOx), Hydrogen chloride gas, Sulphur oxides 11. TOXICOLOGICAL INFORMATION Hazardous components : Glycerol Acute oral toxicity Acute inhalation toxicity Acute dermal toxicity LD50 rat 12,600 mg/kg LC50 rat 1 h > 570 mg/m3 LD50 rat > 21,900 mg/kg Hazardous components : Hydroxylated Organoamine no data available Hazardous components : Dimethyl sulfoxide Acute oral toxicity Acute dermal toxicity LD50 rat 14,500 mg/kg LD50 rat 40 g/kg Further information The product itself has not been tested. Page 4 of 7 Revision Date:

5 12. ECOLOGICAL INFORMATION We have no quantitative data concerning the ecological effects of this product. An environmental hazard cannot be excluded in the event of unprofessional handling or disposal. Component: Glycerol Ecotoxicity effects Toxicity to fish Toxicity to daphnia LC50 Oncorhynchus mykiss (rainbow trout) 96 h 51,000-57,000 mg/l EC50 Daphnia magna (Water flea) 24 h > 500 mg/l Component: Dimethyl sulfoxide Ecotoxicity effects Toxicity to fish flow-through test LC50 Pimephales promelas (fathead minnow) 96 h 34.0 mg/l LC50 Oncorhynchus mykiss (rainbow trout) 96 h g/l LC50 Lepomis macrochirus 96 h 33.5 g/l Toxicity to algae Toxicity to bacteria EC50 Skeletonema costatum 96 h 12,350-25,500 mg/l EC50 Photobacterium phosphoreum 5 min 77 mg/l EC50 Pseudomonas putida 16 h 16,000 mg/l EC50 Tetrahymena pyriformis 24 h 32 g/l Toxicity to daphnia EC50 Daphnia 24 h 7,000 mg/l 13. DISPOSAL CONSIDERATIONS Environmental precautions Disposal methods General advice Should not be released into the environment. Prevent product from entering drains. Dispose of in accordance with local regulations. DISPOSAL CONSIDERATIONS The product has to be disposed of as laboratory chemical in accordance with local regulations. 14. TRANSPORT INFORMATION Page 5 of 7 Revision Date:

6 DOT DOT: ROAD TRANSPORT Not dangerous goods IATA-DGR Not dangerous goods IMDG Not dangerous goods 15. REGULATORY INFORMATION US Regulations 40 CFR Designation of hazardous substances (SARA 302). Non-hazardous substance 40 CFR 355 Appendices A and B - The List of Extremely Hazardous Substances and Their Threshold Planning Quantities (SARA304). Non-hazardous substance 40 CFR Toxic Chemical Release Reporting (SARA 313). Non-hazardous substance State Regulations U.S.- New Jersey - Worker and Community Right to Know Act (N.J.S.A. 34:5A-1) No components regulated under the New Jersey Worker and Community Right-to-Know Act. U.S.- Massachusetts - General Law Chapter 111F (MGL c 111F) - Hazardous Substances Disclosure by Employers (a.k.a. Right to Know Law) No components regulated under the Massachusetts Hazardous Substances Disclosure by Employers Law. U.S. - California - 8 CCR Section Director's List of Hazardous Substances No components found on the California Director's List of Hazardous Substances. U.S. - California - 8 CCR Section Specifically Regulated Carcinogens No components found on the California Specifically Regulated Carcinogens List. U.S. - California - 8 CCR Section 5203 Carcinogens No components found on the California Section 5203 Carcinogens List. U.S. - California - 8 CCR Section 5209 Carcinogens No components found on the California Section 5209 Carcinogens List. California Prop. 65 Not Regulated Page 6 of 7 Revision Date:

7 National Inventory Status - United States of America TOXIC SUBSTANCES CONTROL ACT (TSCA) 8 (B) INVENTORY All the components of this substance are listed on or are exempt from the inventory. For purposes of 40 CFR , this product is for Research and Development (R&D) Use Only TOXIC SUBSTANCES CONTROL ACT, SECTION 12(B) EXPORT NOTIFICATION 40CFR 707, SUBPART D Not Required. 16. OTHER INFORMATION Disclaimer: This MSDS has been prepared using information from sources considered technically reliable. It should not be relied upon as a product specification. The company makes no warranty of any kind, expressed or implied, concerning the safe use of this material in your process or in combination with other chemical substances. Every user of the product is responsible for evaluating its own processes and conditions of use and selecting appropriate protective measures for employee exposure. Document no Print Date 4/25/2009 2:30:00 PM Page 7 of 7 Revision Date: