Novozymes Drain Ease Open

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1 Cleaning Solutions Drain Novozymes Drain Ease Open Application Sheet A clogged drain can stop kitchen operations whether it is a busy restaurant or a dinner for two at home. Drain Ease Open combines fast-acting chemistry to quickly open clogged drains with a patented beneficial microorganism that continuously degrades organic drain buildup. The long-lasting microbial action of Drain Ease Open helps maintain free-flowing drain lines and reduce the organics that can cause malodors. Benefits Fast, effective drain opener Independent laboratory test confirms that Drain Ease Open quickly opens clogged drains and effectively removes congested kitchen soil Helps maintain free-flowing drain lines and prevent drain blockages on a continuous basis by degrading organic compounds found in kitchen drains Reduces the need for additional drain treatment and maintenance Independent laboratory test confirms a Drain Ease Open performance superior to the leading brand Long-lasting odor control Provides long-lasting odor reduction by breaking down odorous volatile fatty acids and odor-causing organic buildup Pleasant lemon fragrance provides instant freshness Patented microbial technology Patented microbial technology for long-lasting grease interceptor performance, even under harsh or low-ph conditions Certification EcoLogo -certified formulation available

2 Product Application Specifications Drain Ease Open Drain opener and maintainer Liquid Fully formulated Ready to use Lemon fragrance Performance Drain Ease Open has been confirmed by independent laboratory testing to open clogged kitchen drains and effectively remove congested kitchen soil made of grease, fat, starch, sugar, and protein. This same independent laboratory s tests show that Drain Ease Open performs better than the leading brand of drain opener. The buildup of organics in the drain line is a major cause of blockages and slow-running kitchen and bathroom drain lines. Figures 1 and 2 show results of the performance of Drain Ease Open on kitchen and bathroom drain soil. The data in Figure 1 show the results of a cleaning performance evaluation of Drain Ease Open on kitchen and bathroom soil spread onto a ceramic tile. 5.0 g (0.18 oz) of Drain Ease Open was left on the kitchen soil stain for 5 minutes and left on the bathroom soil for 30 seconds before rinsing for 15 seconds with water. Drain Ease Open demonstrates good efficacy on kitchen and bathroom soil fixed on a ceramic tile. Figures 2 and 3 shows the results of a cleaning performance evaluation using a mixture simulating drain soil combined with red dye was poured into plastic tubes and left until solid. The flow rate was determined by the time required to let three liters of water pass through the tube. A mineral solution with Drain Ease Open microorganisms was then added to the clogged tubes and changed every 2-3 days. An untreated tube was used as control.

3 Microbial cleaning of kitchen and bathroom soil cleaning Bathroom soil Kitchen soil Before treatment with Drain Ease Open microorganisms Water rinse only Treated with Drain Ease Open microorganisms Fig. 1. Novozymes Drain Ease Open effectively removes both bathroom soil (left) and kitchen soil (right) applied to ceramic tiles. After only 30 seconds of treatment with Drain Ease Open the bathroom soil is easily rinsed off the tile compared to the control (water), which leaves the soil almost unchanged. The same result appears after just 5 minutes of treatment time on the kitchen soil, whereas the control remains the same.

4 Untreated (kitchen soil only) Treated with Drain Ease Open microorganisms Day 0 Day 4 Day 7 Day 10 Fig. 2. Time it took for water to flow through the tubes was measured daily. The time it takes the water to pass through the untreated tube is unchanged after 10 days and the tubes show no change (left). The tube treated with Novozymes Drain Ease Open microorganisms (right) shows almost complete soil removal, and the time for water to flow through the tube improved by 25 seconds.

5 Flow rates (seconds) Day 0 Day untreated tube treated tube Fig. 3. Time to pass three liters of water was measured in both untreated tubes and tubes treated with Novozymes Drain Ease Open microorganisms. The time it takes the water to pass through the untreated tube is unchanged after 10 days. The treated tubes present a significant improvement of flow; at day 0 the water flows through in 47 seconds and at day 10, in 22 seconds. By degrading organics over time, the microorganisms present in Novozymes Drain Ease Open maintaining a free flowing drain line.

6 Drain Ease Open bacteria degrade kitchen soil to prevent drain line clogs A test to demonstrate the ability of bacteria to degrade kitchen soil was performed. To simulate drain soil, a mix of grease, butter, egg yolk, sugar, protein, water and red dye was poured into glass tubes and left until solid in order to block the entire section of tube (Figure 4). The clogged tube section is placed between two bend tubes to simulate a drain line (Figure 5). A mineral solution with Drain Ease Open microorganisms is inserted in the left part of the bend tubes. An untreated tube was used as control. The microbial solution is refreshed every 3-4 days, allowing the addition of essential salts necessary for bacterial metabolism (simulating the salts commonly found in tap water). Clogged tube Fig. 4. Fig. 5. Microbial solution Clog Empty tube The clogged section is continuously degraded until the structure of the clog is weak and thin enough to allow the liquid solution to spread all through the tube. After 12 days, the clog is broken, the red dye is then released thorough both bend tubes, as seen in Figure 6. The untreated tube stays unchanged. This experiment demonstrates clog prevention is possible thanks to the ability of bacteria to continuously degrade kitchen drain soil. Untreated (clogged tube only) Treated with Drain Ease Open microorganisms Day 0 Day 12 Fig. 6. After 12 days the clog treated with Drain Ease Open microoganisms is released. The untreated tube remains unchanged.

7 Long-lasting and continuous activity on kitchen soil with Drain Ease Open microorganisms The data in Figure 7 show that beneficial microorganisms in Drain Ease Open have a respiration activity when inoculated in organic medium simulating greasy kitchen drain soil. Metabolic activity is still observed after 16 days when organics are present. The oxygen uptake indicates the biological conversion of the organics into by-products (organics + O 2 + CO 2 and H 2 O) as well as new bacterial cells. An increase of the oxygen uptake demonstrates that microorganisms have a metabolic activity and degrade organics. Microbial activity on kitchen soil 500 Cumulative oxygen uptake (mg) Time (days) Fig. 7. The long-lasting, continuous degradation (15+ days) of kitchen soil and grease is demonstrated by the respirometric activity of the microorganisms in Novozymes Drain Ease Open.

8 Microbial activity on odorous molecules and buildup The data in Figure 8 show that microorganisms in Drain Ease Open are able to germinate on kitchen drain soil and consume all the organics present. When the organics have been consumed, the microorganisms become dormant (spore state) until a new food source is available. When new food is added in the drain environment, spores regerminate, become active, and continue to consume the organics present. When the food has been consumed, some of the microorganisms die while some become dormant (spores) until a new food source is available. No food Microorganisms with kitchen soil and grease 1.2E E+09 Kitchen soil addition Count (cfu/ml) 8.0E E E+08 Kitchen soil depletion C D B 2.0E+08 A 0.0E Time (days) Fig. 8. The microorganisms in Novozymes Drain Ease Open consume the kitchen soil and become dormant (spores) when all organics have been degraded. Microbial action increases with the addition of a new food source at day 6. This continuous degradation helps keep drain lines free-flowing. Additionally, microscopic observations at 400X magnification of test flasks are made at different stages of microbial growth (Fig. 9). Dormant microorganisms (spores), vegetative (active) microorganisms, vegetative microbes transforming into spores, and vegetative microorganisms are observed. Microbial growth A = day 0 Spores only B = day 3 Vegetative microorganisms C = day 5 Vegetative microbe-forming spores D = day 7 Vegetative microorganisms Fig. 9. Microscopic observation: Microbial growth with Novozymes Drain Ease Open microorganisms in kitchen soil over 7 days shows that microorganisms return to spore (dormant) form in the absence of a food source.

9 Microbial activity on odorous molecules and buildup Odors and buildup associated with drain lines can be a major problem for food service facilities and homes. As shown in Figure 10, the patented beneficial microorganism in Drain Ease Open helps reduce malodors and drain soil buildup by degrading both the short- and the long-chain volatile fatty acid (VFA) compounds found in drains. Microbial activity on odorous molecules 1000 Palmitic acid Cumulative oxygen uptake (mg/l) Valeric acid Butyric acid Acetic acid Stearic acid Oleic acid Time (days) Fig. 10. The ability of a patented beneficial microorganism in Novozymes Drain Ease Open to degrade various fatty acids responsible for malodors or buildup is demonstrated by a respirometry test. The results show that this microbe in Drain Ease Open can degrade both the long- and the short-chain fatty acids commonly found in kitchen drain soil.

10 Recommended use directions Drain Ease Open is a liquid, ready-to-use product for drain lines. Apply directly to the head of the drain line. RESIDENTIAL DRAIN LINES Operation Treatment time Daily dosage Blockage Start-up When water is still in sink oz / ml Slow-running 3-day treatment At bedtime for best results 2 4 oz / ml Maintenance Everyday/ongoing At bedtime for best results 2 oz / 59 ml INSTITUTIONAL DRAIN LINES Blockage Start-up When water is still in sink oz / ml Slow-running 3-day treatment During the least use 4 6 oz / ml Maintenance Everyday/ongoing During the least use 4 oz / 118 ml Safety, handling, and storage Safety, handling, and storage guidelines are provided with all products. Novozymes Biologicals, Inc. Tel.: Corporate Circle Salem VA United States cleaningsolutions@novozymes.com Novozymes is the world leader in bioinnovation. Together with customers across a broad array of industries we create tomorrow s industrial biosolutions, improving our customers business and the use of our planet s resources. Read more at Laws, regulations, and/or third-party rights may prevent customers from importing, using, processing, and/or reselling the products described herein in a given manner. Without separate, written agreement between the customer and Novozymes to such effect, information provided in this document AS IS is of good faith and does not constitute a representation or warranty of any kind and is subject to change without further notice. Novozymes A/S No