Supporting Information

Transcription

1 Supporting Information Characteristic Regions of Fluorescence Excitation-Emission Matrix (EEM) to Identify Hydrophobic/Hydrophilic Contents of Organic Matter in Membrane Bioreactors Kang Xiao a,b, Yuexiao Shen c,*, Shuai Liang d, Jihua Tan a, Xiaomao Wang b, Peng Liang b, Xia Huang b,* a College of Resources and Environment, University of Chinese Academy of Sciences, Beijing , China b State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing , China c Department of Chemistry, University of California, Berkeley, CA 94720, USA d College of Environmental Science and Engineering, Beijing Forestry University, Beijing , China Summary of figures and tables: Figure S1. Procedure for hydrophobic/hydrophilic fractionation of DOM. Page S2 Figure S2. Schematic diagram of scanning EEM regions for fluorescence correlation with hydrophobic/hydrophilic proportions. Page S3 Figure S3. Contours of fluorescence quotient (FQ) between different hydrophobic/hydrophilic components (sample number n = 10). Page S4 Figure S4. Regression of the relationship between HOA proportion and the regional fluorescence proportion of (Ex, Em) = (-310, ) nm with the HOA proportion corrected by excluding the non-fluorescence portion of HIS from the total solution. Page S5 Table S1 Basic information of the 10 WWTPs for DOM sampling. Page S6 Table S2 Influent, effluent and mixed liquor properties of the 10 WWTPs at the sampling time. Page S7 Table S3 Contents of polysaccharides, proteins and humics in HIS, HOA and HOB. Page S8 *Corresponding authors addresses: syxbach@gmail.com (Y. Shen), xhuang@tsinghua.edu.cn (X. Huang). S1

2 DAX resin column with dead volume V0 Figure S1. Procedure for hydrophobic/hydrophilic fractionation of DOM. S2

3 Scanning windows Increasing scanning runs Figure S2. Schematic diagram of scanning EEM regions for fluorescence correlation with hydrophobic/hydrophilic proportions. S3

4 (a) HOA : HIS (b) HOB : HIS (c) HOA : HOB average standard deviation average standard deviation average standard deviation Figure S3. Contours of fluorescence quotient (FQ) between different hydrophobic/hydrophilic components (sample number n = 10). S4

5 TOC proportion of HOA Experimental Fitted line 95% C.I. mean 95% C.I. individual y = 2.176x R 2 = Q 2 = Simulated R Regional fluorescence proportion Frequency (%) Probability of fitted line track by Monte Carlo simulation Figure S4. Regression of the relationship between HOA proportion and the regional fluorescence proportion of (Ex, Em) = (-310, ) nm with the HOA proportion corrected by excluding the non-fluorescence portion of HIS from the total solution. The proportion of non-fluorsecence matter in HIS was tentatively estimated assuming that the fluorophores in HIS have similar fluorescence intensity per TOC as the fluorophores in HOA. S5

6 Table S1 Basic information of the 10 WWTPs for DOM sampling. No. Region of WWTP location Wastewater type Designed capacity (m 3 /d) Process flow a #1 Hai River, North China Domestic 60,000 #2 Tai Lake, East China #3 Hai River, North China Domestic (~40%) + industrial (~60%) Domestic (~80%) + industrial (~20%) 20,000 40,000 #4 Hai River, North China Domestic 30,000 #5 Tai Lake, East China #6 Tai Lake, East China Domestic (~40%) + industrial (~60%) Domestic (~40%) + industrial (~60%) 30,000 20,000 #7 Dian Lake, Southwest China Domestic 60,000 #8 Yangtze River, Central China Domestic 110,000 #9 Hai River, North China #10 Tai Lake, East China Domestic (~80%) + industrial (~20%) Domestic (~60%) + industrial (~40%) 35,000 50,000 a Process flow consists of anaerobic tank (A 1 ), anoxic tank (A 2 ), aerobic tank (O), and membrane tank (M). S6

7 Table S2 Influent, effluent and mixed liquor properties of the 10 WWTPs at the sampling time. a No. MLSS MLVSS Influent (mg/l) Effluent (mg/l) (g/l) (g/l) COD NH 4 + -N TN TP COD NH 4 + -N TN TP # # # # # # # # # # a Data given as average of triplicate measurements. S7

8 Table S3 Contents of polysaccharides, proteins and humics in HIS, HOA and HOB. a Polysaccharide content (g-glucose/g-toc) Protein content (g-bsa/g-toc) Humic content (g-fluka HA/g-TOC) HIS HOA HOB 0.93 (±0.20) 0.29 (±0.08) 0.55 (±0.21) 0.16 (±0.13) 0.10 (±0.08) 0.14 (±0.23) 0.15 (±0.15) 0.50 (±0.18) 0.37 (±0.24) a Data averaged over the 10 WWTPs with standard deviation given in parentheses. S8