Lab Work Safety Awareness Presented by: Joseph Vincelli Operations Manager

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1 Lab Work Safety Awareness Presented by: Joseph Vincelli Operations Manager

2 Mission Statement To support the continuous improvement of the University s safety culture by providing advice, guidance, training, and technical support to the McGill community

3 Regulations & Guidelines Public Health Agency of Canada (PHAC) Guidelines Canadian Food Inspection Agency (CFIA) Standards Environment Canada New Substances Notification Quebec Regulation Respecting Occupational Health and Safety Workplace Hazardous Materials Information System (WHMIS) Transport Canada Transport of Dangerous Goods Canadian Nuclear Safety Commission (CNSC) McGill mylab

4 Regulations P H A C Canadian Biosafety Standards and Guidelines Human Pathogens C F I A Animal Pathogens C F I A Prions

5 CFIA Standards Containment Standards for Facilities Handling Plant Pests Containment Standards for Facilities Handling Aquatic Animal Pathogens (2010)

6 Environment Canada New Substances Notification Regulations (Organisms) Reporting mandatory for manufactured/imported microorganisms if: Not on Domestic Substances List Research & development exemption unless: Introduced into experimental field study without containment

7 Risk Groups Categorization of relative hazards of infective organisms (4 levels) Risk Groups 1, 2, 3 & 4 Risk classification: Pathogenicity Infectious dose Mode of transmission Host range Effective prevention (e.g. vaccine) Effective treatment (e.g., antibiotic, antiviral PEP)

8 Containment Level Minimum requirements for safe handling (4 levels) Operational practices Safe work practices Engineering, technical, physical: Location & access Surface finishes & casework HVAC Containment perimeter (windows, autoclave location, etc.) Services (water, drains, gas, electricity, equipment)

9 Containment Level 1 No special design features Biological safety cabinet not required, may be used for sterility Work may be done on open benchtop Good aseptic/microbiological technique

10 Containment Level 2 Additional physical requirements, e.g. Limited access, signage, lockable doors Resistant, non-absorptive surfaces (for disinfection) Containment of aerosols: Biological safety cabinets Centrifuges with sealed rotors or safety cups Minimize environmental contamination: Handwashing sinks Decontamination facilities (e.g. autoclaves)

11 Containment Levels 3 and 4 Level 3 Respiratory protection HEPA filtration of lab exhaust Strictly controlled access Level 4 Isolated facility with sealed perimeter Positive pressure suits or Class 3 BSC Effluent sterilization system

12 Biohazards Policy* Prior to beginning work with biohazardous material, responsible users must complete and submit an Application to Use Biohazardous Materials to Environmental Health & Safety for review and approval *University Laboratory Safety Committee September 24, 2007

13 WHMIS* Objective To ensure the protection of workers and students from the adverse effects of hazardous materials through the promotion of access to health and safety information on Controlled & Hazardous Products *Workplace Hazardous Materials Information System

14 WHMIS Key Elements Worker core training Chemicals grouped in 6 classes Cautionary labeling of containers Supplier Labels, Workplace Labels, Laboratory Labels Material Safety Data Sheets (MSDS) Provided by the manufacturer/supplier

15 WHMIS - Classes A Compressed Gases D - Poisonous & Infectious Materials D2 -Materials Causing Other Toxic Effects B Flammable & Combustible D - Poisonous & Infectious Materials D3 - Biohazardous Infectious Material C- Oxidizing Material E Corrosive Material D - Poisonous & Infectious Materials D1 - Materials Causing Immediate & Serious Toxic Effects F - Dangerously Reactive Material

16 Transport Canada -TDG Regulations 9 Classes, designated 1 to 9 Chemicals and explosives in classes 1, 2, 3, 4, 5, 6.1, 8 and 9 Infectious substances in class 6.2 Radioactive materials in class 7 Packaging and labeling of biological agents or toxins transported to another entity Mandatory training for all who transport (shipping and receiving)

17 Canadian Nuclear Safety Commission (CNSC) The acquisition, possession and use of radiation sources are subject to licensing by the CNSC EHS administers the CNSC license, renewed every 5 years. Internal permits are issued by EHS to radiation users (renewed yearly) after completing the Application to Use Radioactive Materials Internal permits contain: Name of permit holder; Classification of laboratory (e.g. Basic, Intermediate, High); Validity period; Approved use and/or storage locations; Radioisotope types and possession limits; Certain compliance conditions.

18 Radiation Safety Policies* Compliance Enforcement Policy Radiation Safety "3 Strikes" Enforcement Policy Radioisotope Laboratory Decommissioning Policy Radiation Safety Training Policy McGill Radioisotope Acquisition Policy Pregnancy & Breast-Feeding Policy

19 McGill mylab Web based compliance tool Maintains hazardous material inventories Allows shared inventories Inventory linked to MSDS service (3E Inc.) Critical emergency response information To get started, contact Parisa Parang to setup an account. Local: 8778

20 McGill mylab

21 Definition: Biohazards

22 Biohazards Bacteria Viruses Fungi Parasites Plants and algae Prions

23 Biohazards Toxins of biological origin Human tissue, blood, body fluids Animal tissues, carcasses Nucleic acids Genetically modified strains & organisms Others that could contain biohazardous material, e.g. Cell lines Cultures from soil samples Waste water

24 Radioactive materials Ionizing radiation composed of particles and/or photons or electromagnetic radiation Alpha Particles (α) Beta Particles (β-) Positron (β+) Gamma Radiation (γ) X-Rays Present as an open or sealed source External radiation protection: Time, Distance, Shielding and Contamination Control

25 LAI*: Routes of Transmission Origin: biological, chemical, or radioactive Direct contact (e.g. splash, spills) Inhalation Ingestion Dermal entry Parenteral inoculation (injection) Indirect contact (i.e. touching a contaminated door handle) Vectors: animal/insect bites *Laboratory acquired infections (LAI) or exposures

26 Safe Work Practices Understand hazards Restrict lab access Avoid clutter Use personal protective equipment Lab coat Gloves Eye protection

27 McGill PPE Policy

28 Safe Work Practices (cont d) No shorts, sandals, etc. Universal Precautions with human blood No food, drinks, etc. No mouth pipetting Work carefully, deliberately Decontaminate work surfaces

29 Safe Work Practices (cont d) Use needles, syringes only if necessary Report accidents to Supervisor and/or EHS Cuts, needlesticks, scrapes Contamination of broken skin Splash of hazardous materials Incidents and near accidents Document by completing the McGill Accident/Incident/Occupation Disease form

30 Safe Work Practices (cont d) Consequences of poor work practices: Personnel exposure Contamination of research Environmental release

31 Lab Safety Training Courses WHMIS Training for Lab Personnel Principles of Laboratory Radiation Safety Introduction to Biosafety Safe Use of Biological Safety Cabinets Hazardous Waste Management & Disposal Training for Lab Personnel Laser Safety Course mylab Training Courses & online documents

32 Contact information: Telephone: Fax: Website: Address: 3610 McTavish, 4 th floor