Crest Biotech Pvt. Ltd.

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1 Crest Biotech Pvt. Ltd.

2 PROMOTING SUSTAINABLITY : BIO ETHYL ALCOHOL PRODUCTION

3 PROMOTING SUSTAINABLITY : BIO ETHYL ALCOHOL 1. RAW MATERIALS : PRE REQUISITE 2. TECHNOLOGY : UP GRADATAION 4. POLICY : FINANCE / APPROACH CANE MOLASSES : BIO ETHANOL & CHEMICAL CANE MOLASSES : PURITY TO BE NOT LESS THAN % GRAIN STARCH : POTABLE ALCOHOL SWEET SORGHUM : INCENTIVIES /SECURITY TO FARMERS FERMENTATION, ZELD & BY PRODUCTS DEVELOPMENTS 4 YEARS DEGREE FOR FERMENTATION TECHOLGOY BY NSI/DSI SET UP R & D CENTRE BY AIDA PRIVATE R & D CENTRES, + J/V & GRANTS WITH NSI /DSI & DISTILLERY 3. POLLUTION : PRAGMATIC + IVE APPROACH ( CLOSURE IS DISASTER ) LONG TERM SOLUTIONS BY ENVIRONMENT MINISTRY /CPCB / SPCB 95% ETHYL ALCOHOL AS E5/E10 DOPPING IN PETROL TDF TO BE CREATED FOR DISTILLERY INDUSTRY UNIFORM POLICY CENTRAL AND STATE INTER STATE MOMENT - GST REMUNERATIVE PRICE + + IVE APPROACH BY OIL COMPANIES

4 1. ALTERNATE RAW MATERIALS MOLASSES, GRAIN STARCH, SWEET SOGHUM

5 RAW MATERIALS FOR ETHYL ALCOHOL SUGAR CANE SUGAR BEET CANE MOLASSES AH / BH / C. RAW MATERIALS GRAIN RICE, WHEAT SORGHUM, MILLETS, BARLEY, CORN SWEET SORGHUM JUICE. POTATO SWEET POTATO CASSAVA AGRO RESIDUES CELLULOSE ALGAE 2 ND GENERATION ETHYL ALCOHOL RICE STRAW SOFT CELLULOSE

6 JUICE ALCOHOL. SUGAR CANE MOLASSES 4-5% CANE MOLASSES BH / C MOLASSES PURITY %. PRESS MUD % BIO COMPOST. SUGAR CANE INDUSTRY

7 QUALITY OF RAW MATERIALS FOR ETHYL ALCOHOL QUALITY HARVESTING PROCESSING HANDLING STORAGE SUGAR % RESIDUE / SLUDGE % MICRO BIOLOGY WY / TBC / MOLD PROCESS WATER WY / TBC / MOLD PROCESS AIR WY / TBC / MOLD TASK TO GET QUALITY PREPARATIONS PRE TREATMENT TECHNOLOGY UP GRADATION a solution

8 MOLASSES : HANDLING, STORAGE AND QUALITY??? AH MOLASSES BH C PURITY ½ - 3 BOILED TRANSIT HEATERS SUGAR FACTORY STORAGE OPEN PITS COVERED MS TANKS DISTILLERY STORAGE OPEN PITS COVERED MS TANKS TIS 40 50% HIGH UFS PENTOSES, TREHALOSE MANNON, GLUCAN ETC. HIGH MICROBIAL COUNTS HIGH SLUDGE CONTENT TRANSPORT BY MS TANKERS

9 INHIBITORS IN MOLASSES INHIBITORS FACTOR ,000 40,000 PPM FACTOR 2 2,700 3,500 PPM FACTOR PPM FACTOR 1 CONCENTRATION IS HIGHLY DETRIMENTAL TO GET HIGH ALCOHOL % MORE THAN 10% IN WASH FACTOR 1 PRESENT IN SPENT WASH IN CONCENTRATION OF 11,000 14,000 PPM RESULTS INTO INHIBITION OF YEAST AND VERY POOR FERMENTATION, THEREFORE, SPENT WASH CAN NOT BE RECYCLED AS IT IS.

10 GRAINS : POWDER QUALITY

11 GRAINS : MICROBIAL ANALYSIS PARTICU- LARS UNIT RICE RICE WHEAT NOS/gm UN INCUBATED INCUBATED INCUBATED TBC NOS/gm 2X10 4 ~ 2.1X X10 8 ~ 1.3X X10 8 ~ 1.3X10 11 WY NOS/gm 2.5X10 5 ~ 3.9X X10 5 ~ 7.2X10 7 6X10 4 ~ 2.1X10 6 MOLDS NOS/gm 1.4X10 5 ~ 3.3X X10 5 ~ 2.4X10 8 INCUBATED 24 HRS IN YEPD BROTH & TOTAL PLATE COUNTS ON NA, LYSINE, ASHA MEDIA

12 SWEET SORGHUM IN INDONESIA

13 SWEET POTATO - YAM HIGH IN CARBOHYDRATE CONTENT

14 SWEET SORGHUM : VALUABLE CROP FOR ETHYL ALCOHOL / GREEN ENERGY / FEED / FOOD BAGASSE FUEL STEAM ANIMAL FODDER CANE TOP LEAVES SWEET SORGHUM CO GENERATION POWER SOIL CONSERVATION ROOTS CANE JUICE GRAIN JUICE SYRUP BIO ETHANOL BIO MANURE FILTER MUD BY PRODUCTS DDGS DRY YEAST CONCENTRATED PROTEINS

15 COMPARISON : RAW MATERIALS SUGARCANE, MOLASSES, CASSAVA, CORN & SWEET SORGHUM FOR BIO ETHANOL In terms of feedstock, sweet sorghum is an important alternate raw material for bio ethanol production.

16 2. TECHNOLOGY UP GRADATION

17 UNIQUE FERMENTATION ( UF ) YEAST STRAIN, CREST S ADDITIVES, INTEGRATED FERMENTATION PROCESS & ENGINEERING

18 IMPROVING FERMENTATION EFFICIENCY 87% 95%

19 CREST S YEAST TOLERANCE TO HIGH TEMPERATURE ( C ) HIGH ALCOHOL CONCENTRATION ( %) FAST FERMENTING ( / HRS)

20 CREST S SPECIFIC ADDITIVES BENZYL SEPTOL CREST 1 CREST 2 CREST 3 with optimal Crest s macro & micro nutrients CREST Z

21 FERMENTATION SYSTEMS * CONTINUOUS???? * CASKETING???? * BIO STILL???? * FED BATCH???? systems need to be reviewed in absolute terms? leads to poor and inefficient fermentation due to various reasons??

22 UF SYSTEM ( CANE MOLASSES BATCH ) * CULTURE CREST S YEAST STRAIN * HIGH YEAST CELL CONCENTRATION-BUB * 2 NOS FERMENTERS * 1 NO. WASH OUT TANK OPTIMUM DOSAGES OF MACRO, MICRO NUTRIENTS, FERMENTATION ADDITIVES, OPTIMAL AERATION & INTEGRATED PROCESS STEPS ENGINEERING UPGRADATION OF EXISTING FERMENTATION EQUIPMENTS WELL DESIGNED FERMENTATION EQUIPMENTS

23 GRAIN STARCH : CONTINUOUS FERMENTATION RICE GRAIN STARCH ENZYME + YEAST + NUTRIENTS + DECONTAMINANT PROCESS ENGINEERING ALCOHOL RECOVERY DISTILLATION R.S. ENA A.H. AMBIENT TEMPERATURE FERMENTATION Crest s Integrated Technology

24 UF : GOOD AND EFFICIENT FERMENTATION

25 UF : ADDITIONAL ALCOHOL YIELD 250 LITERS ALCOHOL/ TON 465 LITERS ALCOHOL/ TON ETHANOL YIELD IMPROVED TECHNOLOGY MOLASSES RICE CONVENTIONAL TECHNOLOGY MOLASSES RICE 220 LITERS ALCOHOL/ TON 430 LITERS ALCOHOL/ TON

26 3. ZERO LIQUID EFFLUENT DISCHARGE ( ZELD ) REDUCE EFFLUENT, CLEAR SPW, HIGH DM, MEE CONCENTRATE, RECYCLE CONDENSATES & VALUE BY PRODUCTS

27 REDUCE SPENT WASH GENERATION 1200 KLPD 700 KLPD but problem persists?

28 SEPARATE CALCIUM & YEAST AFTER FERMENTATION CLEAN FERMENTED WASH ( WITHOUT SLUDGE ) results in getting clean spent wash

29 HIGH DRY MATTER CONTENT CLEAN RAW SPENT WASH FEED TO MEE 4-5% 12-15

30 CLEAR RAW SPENT WASH FOR ETP SYSTEMS

31

32 CONTINUOUS FLUID BED DRYER HORIZONTAL FLUID BED DRYER

33 RECYCLE MEE CONDENSATE RECYCLE TOTAL VOLUME OF SPENT LEES & MEE CONDENSATE FOR DILUTION OF RAW MATERIALS FOR NEXT BATCH OF FERMENTATION. *NO EQUIPMENTS, ONLY SIMPLE TREATMENT AND PROCESS STEPS.

34 UTILIZATION OF MEE CONCENTRATE : VALUE ADDED BY PRODUCTS AN APPROACH TO ZELD

35 4. POLICY : FINANACIAL / APPROACH

36 95% ETHYL ALCOHOL USE FOR E5/E10 DOPPING IN PETROL POLICY FOR USE OF ALTERNATE RAW MATERIALS * MOLASSES & PURITY BIO ETHANOL/INDUSTRIAL ALCOHOL * SWEET SORGHUM BIO ETHANOL * AGRO RESIDUE BIO ETHANOL ( 2 ND GENERATION ) * STARCH POTABLE ALCOHOL TDF TO BE CREATED FOR DISTILLERY INDUSTRY UNIFORM POLICY CENTRAL AND STATE INTER STATE MOMENT - GST REMUNERATIVE PRICE + + IVE APPROACH BY OIL COMPANIES

37 Crest s UF & ZELD (a net profit & sustainability for distillery industry ) READY TO TAKE UP TURNKEY PROJECT

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