prepfast Autodilution Autocalibration Auto QC Dilution Elemental Scientific
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1 prepfast prepfast T h e N e x t S t a g e o f A u t o m a t i o n Autodilution Autocalibration Auto QC Dilution E v o l u t i o n o f A u t o m a t i o n I C P I C P M S A A Elemental Scientific
2 The Evolution of Automation.prepFAST prepfast is a dilution system that automatically performs precise and accurate inline dilutions for samples and standards. Samples are rapidly (0.5 ml/sec) and reproducibly vacuum loaded from each autosampler location into a sample loop. From there the sample is injected (within valve) into a diluent liquid stream and transported to a low volume tee located between the valve and nebulizer. Internal standard is added in the tee to obtain final dilution factors between 1x (undiluted) and 200x. At the heart of the system is the S400V syringe pump. Precise (<± 0.05%), accurate (<± 0.2%), smooth and balanced delivery of solution over a wide range of flow rates (1 to 500 μl/min) ensures rapid reliable inline dilutions. Capable of up to 400x dilution, the prepfast is the fastest, simplest way to ensure high quality data in every run S400V Syringe Pump 0.05% syringe precision Syringe Pumping Reproducibility Benefits of Inline Dilution Real time dilutions Reduced reagent consumption No additional tube or reagents required Automates dilutions Rapid uptake and wash out Low contamination risk Sample analysis time constant, independent of dilution factor Volume measured (ml) RSD = 0.05% Sample Number Page 2
3 Autodilution in Seconds.prepFAST Dilutions are performed by accurately controlling the sample and diluent flow rates. Desired dilution factors are achieved in seconds by utilizing in-valve mixing capabilities. Mixing inline eliminates the need to premix/dilute samples in discrete tubes prior to analysis and greatly improves sample throughput. Combined with FAST technology, rapid sample uptake and FAST washout times are independent of dilution factor allowing rapid injection of the next sample. 2500x fold washout of Ag in seconds Benefits of the prepfast system Rapid vacuum loading Inline dilution as sample is injected Rapid uptake and stabilization of sample FAST washout even for memory prone elements Vacuum Sample Internal Standard Carrier Diluent P7+ Inline Mixing Valve PFA-ST Nebulizer All flows are syringe driven S400V Syringe Pump 2011 Elemental Scientific Page 3
4 STABLE Short and Long Term Stability.prepFAST Syringe pumps eliminate peristaltic pump tubing wear and pulsation that can adversely affect both short and long term stability. Sample and diluent delivery is stable over a wide range of (200 fold) mixing ratios. Stability at low sample flow rates provides the greatest inline dilution factors of any commercial sample preparation system available. Constant preset delivery rates of solution by the S400V provide long term signal stability at all dilution factors. < 2.5% RSD over 14 hours Mn RSD = 2.5% Ag RSD = 2.0% Pb RSD = 2.1% Benefits of the prepfast system Eliminates peristaltic pump pulsation Long term stability at all dilution factors 520 syringe steps per μl Long term stability Page 4
5 AUTOMATED Automated Dilutions. prepfast Tedious manual preparation steps are automatically handled by the prepfast system, making better use of time and resources. Automated dilution is utilized for both standard calibration and sample dilution. Predefined dilution factors for a single muti-element standard are used to build calibration curves. Accuracy of dilution is illustrated by excellent linearity of the calibration curve (r=0.9999). Sample dilution can be predefined on a per-sample basis, allowing for a wide range of dilution factors to be used when sample types or elemental concentrations vary greatly. Benefits of the prepfast system Automatically calibrates the instrument from a single standard solution Automatically dilute samples minimizing sample preparation Intensity (cps) Linearity R 2 = Cadmium R² = Concentration (ppb) Depending on ICP/ICP-MS QC functions available, additional benefits of prepfast are Automatically rerun over range samples at appropriate dilution Automatically rerun internal standard failure at appropriate dilution Automatically prescreen sample for appropriate dilution Insert and run priority samples Page 5
6 SIMPLE Intuitive Operation.prepFAST Installation and operation of the prepfast system is straightforward. The system consists of an SC-DX series autosampler, an P7+ inline mixing valve, a S400V syringe pump and associated plumbing. The prepfast software and hardware is integrated seamlessly with your ICP or ICPMS. Operation is intuitive and once setup for autodilution, transparent to the user. To dilute samples, simply enter the desired dilution for each standard or sample. Load the desired sample file into the ESI SC software. Then the prepfast automatically reads the desired dilution for each sample or standard and performs appropriate inline dilutions. Ready to run in 2 mouse clicks Benefits of the prepfast system Easy installation Rapid implementation Intuitive operation Fully automated Completely integrated Page 6
7 PRECISE High Fidelity.prepFAST Precision is the cornerstone of all high quality data. The ability to discern small variations in data sets leads to greater insight and interpretation. Delivering sample diluent and internal standard via the S400V ensures precise dilution factors and unprecedented signal stability over a wide range of flow rates. Precise results at all dilutions (<2% RSD) 5 4 Benefits of the prepfast system S400V precision syringe Very reproducible flow rates 520 steps per μl, precise control of sample at very low flow rates Specially designed P7+ inline mixing valve and tee Eliminate peripump tubing 1643e Measurements with prepfast vs CRM prepfast ppb CRM ppb Mg 8048 ± ± 98 K 2024 ± ± 29 Al 141 ± ± 8.6 Cr 20.3 ± ± 0.24 Fe 97.7 ± ± 1.4 Co 27 ± ± 0.32 Zn 78.9 ± ± 2.2 Cd 6.56 ± ±.07 Tl 7.49 ± ± 0.1 RSD (%) n = e CRM Mg (100x) K (50x) Cd (0x) Analyte (Dilu1on Factor) prepfast ICPMS Results Page 7
8 ACCURATE On Target Every Time.prepFAST Precise measurements, accurate calibrations, and accurate sample dilutions are necessary to provide accurate concentration values for unknown samples. The accuracy of prepfast syringe-driven (S400V) inline dilutions is illustrated by linear (R 2 =0.9999) calibration curves. Accuracy is further demonstrated by the determination of metal concentrations in a fresh water Certified Reference Material (CRM, NIST 1643). Greatest accuracy is achieved when unknown samples fall in the center of a calibration curve. prepfast is used to appropriately dilute a wide range of element concentrations (Mg 8037 to Cd 7.45 ppb) to obtain fully automated accurate results at any dilution factor. If ICP/ICP-MS software outputs over range QC functions, prepfast will automatically rerun and appropriately dilute samples that are beyond the calibration range; thereby eliminating in-run QC over range failures. Intensity (cps) Calibrate using 1 stock standard Cadmium R² = Concentration (ppb) Benefits of the prepfast system Accurately mixes sample diluent and internal standard Automatically calibrates the instrument from a single standard solution Automatically dilute samples Accurate results at any dilution (CRM 1643e) Depending on ICP/ICP-MS QC functions available, additional benefits of prepfast are Automatically rerun over range samples at appropriate dilution Automatically rerun internal standard failure at appropriate dilution Automatically prescreen sample for appropriate dilution Page 8
9 EFFICIENT Improve Productivity prepfast The prepfast system produces high quality data while enhancing laboratory productivity and profitability. By eliminating manual sample dilution steps, not only is human error removed, but ICP/ ICP-MS operators have more time to focus on instrument setup and data reduction. prepfast can thereby greatly improve efficiency of both instrument and operator time, leading to lower per sample costs. Benefits of Inline Dilution FAST analysis reduces uptake and wash times, saving instrument time Eliminates manual dilutions, saving operator time Reduce reagent use by only diluting volume analyzed Reduce laboratory supplies, test tubes, bottles and pipette tips Eliminate peripump tubing for sample delivery Greatly reduce QC failure and reruns Remove human error from solution preparation Improve productivity and reduce cost per sample Average Sample Run Time (sec) Faster than a peripump Regular Injec3on prepfast FAST with Triple Rinse FAST ICPMS Environmental Analysis Page 9
10 prepfast Inline Capabilities Prescriptive dilution Autocalibration QC over range failure QC internal standard failure Prescreen dilution factors Priority sample dilution Automated standard additions Automated isotope dilution Elemental Scientific E v o l u t i o n o f A u t o m a t i o n Contact us by phone at or by at esi@icpms.com. Our years of experience in the laboratory and in the field are at your disposal to help answer your questions related to elemental analysis. We are pleased to provide our customers complimentary analytical advice from our on-staff chemists. Page 10 U.S. Business Hours: Address: Phone: Central Time Zone 1500 North 24th Street Monday through Friday Omaha, NE :00 am to 6:00 pm Website: Fax: prepfast@icpms.com
11 NOTES:
12 prepfast E v o l u t i o n o f A u t o m a t i o n I C P I C P M S A A Page 12 B-PF-2013-E
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