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2 The powerful new Dionex AS provides a full suite of automation capabilities that elevates laboratory productivity to new heights. Flexible, customizable, and easy to use, the AS establishes a new standard for laboratory automation performance. AS Autosampler Advanced Features erform full-loop, partial-loop, limited sample, sample preparation, sample dilution, precolumn derivatization, matrix elimination, sample preconcentration, and more. With so much application flexibility, even your most difficult analytical challenges can now be fully automated. Simultaneous and Sequential Analysis Double sample throughput with one autosampler. The AS runs both simultaneous and sequential analysis to dramatically improve laboratory productivity. The simultaneous injection technique (Figure ) speeds analysis by using one AS to supply a single sample to two systems running unique or similar applications. Sequential analysis (Figure 2) introduces two unique samples to two systems running unique or similar applications using only one AS. Application Flexibility Since your application needs are unique, the AS can be configured to accommodate even the most complex matrix. From 0.25 m to 0 m syringes, from 0 m vials to 0.45 m well plates, you now have the flexibility to mix and match features to suit your needs. The sample preparation option automates dilution, internal standard addition, and precolumn derivatization, simplifying complex methods. In addition, since all wetted surfaces are inert, there is no need to compromise when it comes to running metal-sensitive s amples or corrosive buffers. Sample Concentration/Matrix Elimination Achieve the lowest possible detection limits on your most difficult analysis with sample concentration/ matrix elimination. With these powerful sample preparation tools, even the most complex tasks can now be automated. The AS simplifies each step with powerful Chromeleon Wizards and the option of front panel control. recise erformance The AS provides accurate and consistent performance for all your sampling needs. From small sample volumes to large-loop or concentrator loading, delivery of sample is precise, reliable, and accurate with every injection and operation. Temperature Control Temperature control is ideal for thermally sensitive samples including carbohydrates, amino acids, organic acids, proteins, peptides, and nucleic acids. The AS reliably controls the sample environment to ensure that even the most reactive samples are safely maintained in the sample tray. Fully Inert eek Needle and Flow ath Only Dionex provides a completely inert pathway for your sample. The unique EEK needle design protects sensitive samples from metallic contamination and ensures compatibility with KEY Flush Air Eluent Sample Diverter Valve S 2 S 2 S 2 W S W S W S W S C C C C W S C W S C W S C To Inject Valve # To Inject Valve #2 ump ump ump ump ump ump ump ump OAD OAD Inject Valve # Inject Valve #2 Inject Valve # Inject Valve #2 Inject Valve # Inject Valve #2 OAD Inject Valve # Inject Valve # Schematic of simultaneous injection, using one AS with two systems. Schematic of sequential injection using one AS with two systems.
3 AS40 Autosampler The Dionex AS40 provides features that you would expect in a highend autosampler yet with a much lower price. Affordable automation is now within your reach without sacrificing performance. Automate difficult applications: The chromatogram illustrates the use of the AS in determining low concentrations of perchlorate (5 ppb) via sample preconcentration. In this example, 5 m of sample were injected over a concentrator column (Cryptand C) to achieve the desired detection limits. RSDs for perchlorate at this concentration are less than %. aqueous and reversed-phase eluents. Reliability oad the AS and walk away. With fully integrated communication feedback and audit trails, the AS performs every assigned automation task and tracks each activity. Equipped with rugged, electrically actuated Rheodyne valves, the AS AS Features: Syringe Size 250, 500, 000, 5000, and 0000 µ Injection Electrically actuated Rheodyne valves Vial Size 0 m polystyrene vials.5 m glass vials 0.3 and.5 m polypropylene vials 0.45, 0.5, and 2.0 m well plates Sample Capacity 0 m vials 49 samples 0.3 or.5 m vials 00 samples 0.45, 0.5, or 2 m wells 92 samples (two well plates, 96 wells per plate) Heat/Cool 4 60 ºC Flow ath EEK / Teflon owerful and Easy to Operate The AS40 is a breeze to use with one-button start-stop operation. Enjoy program-free logic that automates your sample tasks. It s easy. oad your samples, press start, and your analysis begins. The AS40 takes the guesswork out of autosampler programming through simple serial sample loading. Automatically Filters as It Samples The AS40 offers a unique sample filter cap. Each sample can be filtered during loading by an optional 20 µm filter in the vial cap. This unique design removes the tedious task of individually prefiltering samples, saving time and labor. Caps without filters are also available for particulatefree samples. Sample Concentration With unique positive displacement, the AS40 is able to concentrate up to 5 m of sample through a concentrator column. Achieve lower detection limits with no additional sample preparation. oad, concentrate, and review the results. The AS40 does the rest. Chemically Inert The AS40 is entirely chemically inert. All wetted surfaces are non-metallic and designed to work with a large variety of samples, from deionized water to extreme ph chemicals, reagents, and buffers. Inert materials ensure that sample contamination is minimized and that hardware life is extended. ow rofile With one of the smallest autosampler footprints available, the AS40 occupies little bench space, yet has the features you need to support your analyses, with reliability, speed, and precision. Applications Simultaneous and sequential injection, concentration, matrix elimination AS40 Features: Injection Type Vial Size Sample Capacity Sample Filtration Flow ath Applications Displacement into existing injection valve 0.5 m and 5 m olyvials 5 m vials 66 samples 0.5 m vials 88 samples Optional caps with or without filters EEK / Teflon Simultaneous injection, concentration, matrix elimination Well plates, designed for analysts in the life sciences, facilitate processing of large numbers of samples.
4 The Dionex AS-HV simplifies low-level ion analysis with state-of-the-art design, customizable fluidics, and trace-compatible materials. AS-HV Autosampler High-Volume Samples When higher sample volumes are required for trace analysis, matrix elimination, or automated sample preparation, the AS-HV delivers with ease of use, reliability, and flexibility. The AS-HV provides superior functionality in a very compact footprint, and accommodates sample volumes up to 250 m. Optimized Automation Using a unique pirouette arm motion, the AS HV provides powerful automation for many sampling requirements. Rapid, precise arm motion speeds up sampling time to maximize efficiency. The X, θ, Z movement of the AS-HV arm minimizes the mechanical motion typical of autosamplers and thus increases the reliability and ease of operation of the unit. ATC ump 2 DI Water Fully Inert eek Needle and Flow ath With all EEK fluidics, the AS-HV achieves the lowest possible level of detection by using the cleanest materials on all wetted surfaces. The needle and flow path are compatible with a wide range of eluents and samples, from deionized water to extreme ph chemicals, reagents, and buffers. Optional Sample Delivery umps Not all applications are created equal; therefore, the AS-HV supports syringe pump, single piston, and peristaltic pump sampling options. Autorep Option for Trace Analysis Sample and standard handling and dilution can introduce contamination and unintentional variations. The Autorep system works in combination with the AS-HV autosampler and ICS-3000 system to automatically calibrate the system using just a single stock standard. recise valve switching ensures accurate calibration curves with coefficients of determination typically better Suppressor Cell than 99.9%. Automated injection and concentration of the sample prevents dilution errors and exposure to environmental contamination. Optional trace analysis cover Extremely clean samples require isolation and protection from ambient contamination. The AS-HV can be isolated with a protective cover that can be purged with ultrahigh purity gases. Trace Grade Vials When detection limits count, rely on low contaminant tissue culture flasks or Nalgene poly bottles from Dionex. Available in two configurations, polystyrene and polyethylene, each sample vessel configuration is fully supported with optional septa that can be pierced with any supported probe. Use these septa to ensure that no atmospheric or other contamination comes into contact with the sample. Fully integrated Chromeleon Control To ensure maximum productivity, the AS-HV is fully controlled using the advanced features that you have come to expect from Chromeleon. Standard Small oop eristaltic ump (AS-HV) Sample (AS-HV) arge oop 2 ump Eluent The large loop is loaded with sample in pull mode using the Autorep system and the AS-HV autosampler Analytical Guard Concentrator AS-HV Features arameter Injection Type Vial Size Sample Capacity Injections per Vial Flow ath Sample oading Applications Specification Full loop / concentrator 250 m 250 m Tissue culture flasks 24 samples 250 m Narrow mouth Nalgene bottles 5 samples Variable EEK ush or pull Simultaneous injection, concentration, matrix elimination
5 Ordering Information In the U.S., call or contact the Dionex regional office nearest you. Outside the U.S., order through your local Dionex office or distributor. Refer to the following part numbers. AS Autosampler art Number AS40 Autosampler art Number AS Autosampler only AS with sample prep AS with sample tray temperature control AS with sample prep and sample tray temperature control AS with internal injection valve AS with internal injection valve and sample tray temperature control Simultaneous Injection AS with no injection valves Simultaneous Injection AS with sample prep and no injection valves Simultaneous Injection AS with 0630 two internal injection valves AS 0 m vial plastic tray, 49 vial capacity AS 2 m vial tray, 99 vial capacity AS 2 m vial insulated cast tray with covers vial capacity Micro-well plate tray Includes two well plates with round (U) shaped 0.5 m wells and covers Micro-well plate tray Includes two well plates with conical (V) shaped 0.45 m wells and covers Deep-well plate tray Includes two 2 m well plates and covers Vial kit, 00 ea. 0 m polystyrene with caps and septa Vial kit, 00ea..5 m glass with caps and septa Vial kit, 00 ea..5 m polypropylene with caps and septa Vial kit, 00 ea. 0.3 m polypropylene with caps and septa AS40 with 5-m Starter Vial Kit* AS40 with 0.5-m Starter Vial Kit* *AS40 Starter Vial Kits include 2 cassettes and one box of vials with filter caps. AS40, acid resistant, with 5 m Starter Vial Kit Six-position cassettes, box of six, for 5 m vials Eight-position cassettes, box of six, for 0.5 m vials olyvials and filter caps, 250 each, for 5 m vials 0384 for 0.5 m vials olyvial and plain caps, 250 each, for 5 m vials Sample Tip Replacement Kit eak erformance Kit AS-HV Autosampler art Number AS-HV with 24 osition Rack for 250 m Syringe ump Option AX ump Option External eristaltic ump Trace Analysis Cover Sample robe Kit EEK 0645 Sample robe Kit TFE ined and Sleeved Additional Sample Rack 250 m Nunclon 24 osition Additional Sample Rack 250 m Nalgene oly Bottles 5 osition 250 m Tissue Culture Flask Nunc (kg of 50) Caps and Septa for Tissue Culture Flask (kg of 50) m Nalgene Bottle Narrow Mouth (kg of 72) Caps and Septa for 250 m Nalgene Bottle Narrow Mouth (kg of 72) Autorep art Number Autorep System For ICS-3000 DC with installed Automation Manager. Includes sample and standard loops Autorep System plus Automation Manager For new installations or ICS-3000 DC without installed Automation Manager. Includes pre-plumbed Automation Manager, sample and standard loops, and 0-port valve Autorep Installation (recommended)
6 Dionex s goal is to provide you with solutions to your chromatographic challenges rather than just selling you an instrument. You get a complete solution with an open future, and a partner committed to your success. To meet that goal, we offer a complete line of training, service, and consulting products, installation and warranty service, and comprehensive support programs. Quality roducts from a Global eader in Separation Science Dionex provides an extensive array of innovative, high-quality instruments, software, consumables, and associated products that solve problems for laboratories. All Dionex products are designed, developed, tested, and manufactured in accordance with life cycle processes modeled after ISO 900. If You Need Help Dionex offers a range of hardware and software training courses to help you get the most from your system. Whether you need basic training for new operators or advanced training to further the skills of experienced operators, we have the training courses to meet your needs. Training courses are held regularly at Dionex facilities, and can also be held at your organization including custom training. To protect your investment, we offer extended warranties and software subscriptions that keep your system up to date. Find Out More For more information, demonstrations, and no-obligation quotations, contact your local Dionex representative. Corporate Headquarters Dionex Corporation 228 Titan Way.O. Box 3603 Sunnyvale, CA Tel: (408) Fax: (408) Worldwide Sales and Service North America U.S. (847) Canada (905) South America Brazil (55) Europe Austria (43) Benelux (3) (32) Denmark (45) France (33) Germany (49) Ireland (353) Italy (39) Sweden (46) Switzerland (4) United Kingdom (44) Asia acific Australia (6) China (852) India (9) Japan (8) Korea (82) Singapore (65) Taiwan (886) Dionex products are designed, developed, and manufactured under an ISO 900 Quality System Dionex Corporation Chromeleon is a registered trademark of Dionex Corporation. Teflon is a registered trademark of E.I. du ont de Namours. EEK is a trademark of Victrex C. Nalgene is a registered trademark of Nalge Nunc International. N M 07/08 rinted in U.S.A. assion. ower. roductivity.
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