Safety Data Sheets. ZytoLight FISH-Tissue Implementation Kit

Transcription

1 s ZytoLight FISH-Tissue Implementation Kit Product code: Z-2028 Overview on Safety Data Sheets included The document includes Safety Data Sheets for the following kit components: Code PT-0001 ES-0001 WB-0001 WB-0002 MT-0007 Component Heat Pretreatment Solution Citric Pepsin Solution Wash Buffer SSC 25x Wash Buffer A DAPI/DuraTect-Solution GB - EN

2 Heat Pretreatment Solution Citric Print date: Product code: PT-0001 Page 1 of 8 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Heat Pretreatment Solution Citric 1.2. Relevant identified uses of the substance or mixture and uses advised against Use of the substance/mixture The product is intended for professional use Details of the supplier of the safety data sheet Company name: Street: Fischkai 1 Place: D Bremerhaven Telephone: +49 (0) 471/ Telefax:+49 (0) 471/ info@zytovision.com Internet: Emergency telephone number: SECTION 2: Hazards identification 2.1. Classification of the substance or mixture +49 (0) 471/ am - 5pm (CET) Regulation (EC) No. 1272/2008 Hazard categories: Respiratory or skin sensitisation: Skin Sens. 1 Hazard Statements: May cause an allergic skin reaction Label elements Regulation (EC) No. 1272/2008 Hazard components for labelling Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) Signal word: Pictograms: Warning Hazard statements H317 May cause an allergic skin reaction. Precautionary statements P261 Avoid breathing dust/fume/gas/mist/vapours/spray. P272 Contaminated work clothing should not be allowed out of the workplace. P280 Wear protective gloves/protective clothing/eye protection/face protection. P302+P352 IF ON SKIN: Wash with plenty of water. P333+P313 If skin irritation or rash occurs: Get medical advice/attention. P362+P364 Take off contaminated clothing and wash it before reuse Other hazards SECTION 3: Composition/information on ingredients 3.2. Mixtures Revision No: 9,1 - Replaces version: 6 GB - EN Revision date:

3 Heat Pretreatment Solution Citric Print date: Product code: PT-0001 Page 2 of 8 Hazardous components CAS No Chemical name EC No Index No REACH No Classification according to Regulation (EC) No. 1272/2008 [CLP] Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) Acute Tox. 3, Acute Tox. 3, Acute Tox. 3, Skin Corr. 1B, Skin Sens. 1, Aquatic Acute 1, Aquatic Chronic 1; H331 H311 H301 H314 H317 H400 H410 Full text of H and EUH statements: see section 16. Quantity < 20 % SECTION 4: First aid measures 4.1. Description of first aid measures General information Remove contaminated clothing immediately and dispose of safely. In case of accident or unwellness, seek medical advice immediately (show directions for use or safety data sheet if possible). First aider: Pay attention to self-protection! After inhalation Provide fresh air. In all cases of doubt, or when symptoms persist, seek medical advice. After contact with skin After contact with skin, wash immediately with polyethylene glycol, followed by plenty of water. Take off immediately all contaminated clothing and wash it before reuse. Medical treatment necessary. After contact with eyes Rinse immediately carefully and thoroughly with eye-bath or water. In case of eye irritation consult an ophthalmologist. After ingestion Rinse mouth immediately and drink plenty of water Most important symptoms and effects, both acute and delayed 4.3. Indication of any immediate medical attention and special treatment needed Treat symptomatically. No special measures are necessary. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Co-ordinate fire-fighting measures to the fire surroundings Special hazards arising from the substance or mixture Non-flammable Advice for firefighters Wear a self-contained breathing apparatus and chemical protective clothing. Full protection suit. Additional information Suppress gases/vapours/mists with water spray jet. Suppress gases/vapours/mists with water spray jet. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Provide adequate ventilation. Do not breathe gas/fumes/vapour/spray. Avoid contact with skin, eyes and clothes. Use personal protection equipment. See protective measures under point 7 and 8. Revision No: 9,1 - Replaces version: 6 GB - EN Revision date:

4 Heat Pretreatment Solution Citric Print date: Product code: PT-0001 Page 3 of Environmental precautions No special environmental measures are necessary Methods and material for containment and cleaning up Absorb with liquid-binding material (e.g. sand, diatomaceous earth, acid- or universal binding agents). Treat the recovered material as prescribed in the section on waste disposal Reference to other sections Safe handling: see section 7 Personal protection equipment: see section 8 Disposal: see section 13 SECTION 7: Handling and storage 7.1. Precautions for safe handling Advice on safe handling Avoid contact with skin, eye and clothing. Advice on protection against fire and explosion No special fire protection measures are necessary. Further information on handling Handle and open container with care Conditions for safe storage, including any incompatibilities Requirements for storage rooms and vessels Keep container tightly closed. Advice on storage compatibility Keep only in the original container in a cool, well-ventilated place. Further information on storage conditions Store, tightly closed, in the original vessel at temperatures between 2 C and 8 C Specific end use(s) The product is intended for professional use. SECTION 8: Exposure controls/personal protection 8.1. Control parameters 8.2. Exposure controls Protective and hygiene measures Remove contaminated, saturated clothing immediately. Draw up and observe skin protection programme. Wash hands and face before breaks and after work and take a shower if necessary. When using do not eat or drink. Keep away from food, drink and animal feedingstuffs. Avoid contact with skin, eye and clothing. The quality of the protective gloves resistant to chemicals must be chosen as a function of the specific working place concentration and quantity of hazardous substances. Eye/face protection Wear eye/face protection. Hand protection When handling with chemical substances, protective gloves must be worn with the CE-label including the four control digits. The quality of the protective gloves resistant to chemicals must be chosen as a function of the specific working place concentration and quantity of hazardous substances. For special purposes, it is recommended to check the resistance to chemicals of the protective gloves mentioned above together with the supplier of these gloves. Skin protection Wear suitable protective clothing. Revision No: 9,1 - Replaces version: 6 GB - EN Revision date:

5 Heat Pretreatment Solution Citric Print date: Product code: PT-0001 Page 4 of 8 Respiratory protection In case of inadequate ventilation wear respiratory protection. SECTION 9: Physical and chemical properties 9.1. Information on basic physical and chemical properties Physical state: Colour: Odour: liquid colourless odourless ph-value (at 20 C): Changes in the physical state Melting point: Initial boiling point and boiling range: Sublimation point: Softening point: Pour point: Flash point: Sustaining combustion: Flammability Solid: Gas: Explosive properties Lower explosion limits: Upper explosion limits: Ignition temperature: Auto-ignition temperature Solid: Gas: Decomposition temperature: Oxidizing properties Vapour pressure: Density: Bulk density: Water solubility: Solubility in other solvents Partition coefficient: Viscosity / dynamic: Viscosity / kinematic: Flow time: Vapour density: Evaporation rate: No data available Revision No: 9,1 - Replaces version: 6 GB - EN Revision date:

6 Heat Pretreatment Solution Citric Print date: Product code: PT-0001 Page 5 of 8 Solvent separation test: Solvent content: 9.2. Other information Solid content: SECTION 10: Stability and reactivity Reactivity Product has not been tested Chemical stability If stored correctly, the product is stable at least until the expiry date printed on the label Possibility of hazardous reactions Product has not been tested Conditions to avoid Product has not been tested Incompatible materials Product has not been tested Hazardous decomposition products Product has not been tested. SECTION 11: Toxicological information Information on toxicological effects Acute toxicity CAS No Chemical name Exposure route Dose Species Source Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) oral dermal LD50 LD mg/kg 2800 mg/kg inhalative vapour ATE 3 mg/l inhalative aerosol ATE 0,5 mg/l Irritation and corrosivity Causes burns. Rat Rabbit Sensitising effects May cause an allergic skin reaction. (Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1)) Carcinogenic/mutagenic/toxic effects for reproduction STOT-single exposure STOT-repeated exposure Aspiration hazard Revision No: 9,1 - Replaces version: 6 GB - EN Revision date:

7 Heat Pretreatment Solution Citric Print date: Product code: PT-0001 Page 6 of 8 Additional information on tests The mixture is classified as hazardous according to regulation (EC) No 1272/2008 [CLP]. SECTION 12: Ecological information Toxicity Product has not been tested Persistence and degradability Product has not been tested Bioaccumulative potential Product has not been tested Mobility in soil Product has not been tested Results of PBT and vpvb assessment Product has not been tested Other adverse effects Product has not been tested. Further information Avoid release to the environment. SECTION 13: Disposal considerations Waste treatment methods Advice on disposal Do not allow to enter into surface water or drains. Dispose of waste according to applicable legislation. Contaminated packaging Wash with plenty of water. Completely emptied packages can be recycled. Water (with cleaning agent). SECTION 14: Transport information Land transport (ADR/RID) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Other applicable information (land transport) No dangerous good in sense of these transport regulations. Inland waterways transport (ADN) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Other applicable information (inland waterways transport) No dangerous good in sense of these transport regulations. Marine transport (IMDG) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Revision No: 9,1 - Replaces version: 6 GB - EN Revision date:

8 Heat Pretreatment Solution Citric Print date: Product code: PT-0001 Page 7 of 8 Other applicable information (marine transport) No dangerous good in sense of these transport regulations. Air transport (ICAO) UN number: UN proper shipping name: Transport hazard class(es): Packing group: Other applicable information (air transport) No dangerous good in sense of these transport regulations Environmental hazards ENVIRONMENTALLY HAZARDOUS: Special precautions for user Transport in bulk according to Annex II of Marpol and the IBC Code No dangerous good in sense of these transport regulations. SECTION 15: Regulatory information - no Safety, health and environmental regulations/legislation specific for the substance or mixture National regulatory information Employment restrictions: Water contaminating class (D): Skin resorption/sensitization: Chemical safety assessment Observe restrictions to employment for juvenils according to the 'juvenile work protection guideline' (94/33/EC). - - not water contaminating Causes allergic hypersensitivity reactions. Chemical safety assessments for substances in this mixture were not carried out. SECTION 16: Other information Changes This data sheet contains changes from the previous version in section(s): 2,3,4,5,6,7,8,9,10,11,12,13,14,15,16. Abbreviations and acronyms ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage of Dangerous Goods by Road ) IMDG: International Maritime Code for Dangerous Goods IATA: International Air Transport Association GHS: Globally Harmonized System of Classification and Labelling of Chemicals EINECS: European Inventory of Existing Commercial Chemical Substances ELINCS: European List of Notified Chemical Substances CAS: Chemical Abstracts Service LC50: Lethal concentration, 50% LD50: Lethal dose, 50% Relevant H and EUH statements (number and full text) H301 Toxic if swallowed. H311 Toxic in contact with skin. H314 Causes severe skin burns and eye damage. H317 May cause an allergic skin reaction. H331 Toxic if inhaled. H400 Very toxic to aquatic life. H410 Very toxic to aquatic life with long lasting effects. Revision No: 9,1 - Replaces version: 6 GB - EN Revision date:

9 Heat Pretreatment Solution Citric Print date: Product code: PT-0001 Page 8 of 8 Further Information The above information describes exclusively the safety requirements of the product and is based on our present-day knowledge. The information is intended to give you advice about the safe handling of the product named in this safety data sheet, for storage, processing, transport and disposal. The information cannot be transferred to other products. In the case of mixing the product with other products or in the case of processing, the information on this safety data sheet is not necessarily valid for the new made-up material. (The data for the hazardous ingredients were taken respectively from the last version of the sub-contractor's safety data sheet.) Revision No: 9,1 - Replaces version: 6 GB - EN Revision date:

10 Revision date: Pepsin Solution Product code: ES-0001 Page 1 of 8 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Pepsin Solution 1.2. Relevant identified uses of the substance or mixture and uses advised against Use of the substance/mixture The product is intended for professional use Details of the supplier of the safety data sheet Company name: Street: Fischkai 1 Place: D Bremerhaven Telephone: +49 (0) 471/ Telefax:+49 (0) 471/ info@zytovision.com Internet: Emergency telephone number: SECTION 2: Hazards identification 2.1. Classification of the substance or mixture +49 (0) 471/ am - 5pm (CET) Regulation (EC) No. 1272/2008 This mixture is not classified as hazardous in accordance with Regulation (EC) No. 1272/ Label elements Regulation (EC) No. 1272/2008 Special labelling of certain mixtures EUH208 Contains Pepsin A. May produce an allergic reaction. EUH210 Safety data sheet available on request. < 20 % of the mixture consists of ingredient(s) of unknown acute toxicity (inhalation) Other hazards SECTION 3: Composition/information on ingredients 3.2. Mixtures Hazardous components CAS No Chemical name EC No Index No REACH No Classification according to Regulation (EC) No. 1272/2008 [CLP] Quantity methanol < 20 % X Flam. Liq. 2, Acute Tox. 3, Acute Tox. 3, Acute Tox. 3, STOT SE 1; H225 H331 H311 H301 H370 ** TRIS < 20 % Skin Irrit. 2, Eye Irrit. 2, STOT SE 3; H315 H319 H335 Full text of H and EUH statements: see section 16. SECTION 4: First aid measures Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

11 Revision date: Pepsin Solution Product code: ES-0001 Page 2 of Description of first aid measures After inhalation Provide fresh air. If breathing is irregular or stopped, administer artificial respiration. Medical treatment necessary. After contact with skin After contact with skin, wash immediately with polyethylene glycol, followed by plenty of water. Take off immediately all contaminated clothing and wash it before reuse. If skin irritation occurs: Get medical advice/attention. After contact with eyes Rinse immediately carefully and thoroughly with eye-bath or water. After ingestion Rinse mouth immediately and drink plenty of water Most important symptoms and effects, both acute and delayed 4.3. Indication of any immediate medical attention and special treatment needed Treat symptomatically. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Co-ordinate fire-fighting measures to the fire surroundings Special hazards arising from the substance or mixture Non-flammable Advice for firefighters In case of fire: Wear self-contained breathing apparatus. Additional information Collect contaminated fire extinguishing water separately. Do not allow entering drains or surface water. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Use personal protection equipment Environmental precautions Do not allow to enter into surface water or drains Methods and material for containment and cleaning up Absorb with liquid-binding material (e.g. sand, diatomaceous earth, acid- or universal binding agents). Treat the recovered material as prescribed in the section on waste disposal Reference to other sections Safe handling: see section 7 Personal protection equipment: see section 8 Disposal: see section 13 SECTION 7: Handling and storage 7.1. Precautions for safe handling Advice on safe handling No special measures are necessary. Advice on protection against fire and explosion No special fire protection measures are necessary Conditions for safe storage, including any incompatibilities Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

12 Revision date: Pepsin Solution Product code: ES-0001 Page 3 of 8 Requirements for storage rooms and vessels Keep container tightly closed. Advice on storage compatibility No special measures are necessary. Further information on storage conditions Store, tightly closed, in the original vessel at conditions indicated on the label Specific end use(s) The product is intended for professional use. SECTION 8: Exposure controls/personal protection 8.1. Control parameters Exposure limits (EH40) CAS No Substance ppm mg/m³ fibres/ml Category Origin Ethanol TWA (8 h) WEL - - STEL (15 min) WEL Methanol TWA (8 h) WEL DNEL/DMEL values CAS No Substance STEL (15 min) WEL DNEL type Exposure route Effect Value TRIS Worker DNEL, long-term dermal systemic 166,7 mg/kg bw/day Worker DNEL, long-term inhalation systemic 117,5 mg/m³ PNEC values CAS No Substance Environmental compartment TRIS Micro-organisms in sewage treatment plants (STP) Value 300 mg/l 8.2. Exposure controls Protective and hygiene measures Take off contaminated clothing. Wash hands before breaks and after work. When using do not eat or drink. Eye/face protection Wear eye/face protection. Hand protection When handling with chemical substances, protective gloves must be worn with the CE-label including the four control digits. The quality of the protective gloves resistant to chemicals must be chosen as a function of the specific working place concentration and quantity of hazardous substances. For special purposes, it is recommended to check the resistance to chemicals of the protective gloves mentioned above together with the supplier of these gloves. Skin protection Wear suitable protective clothing. Respiratory protection In case of inadequate ventilation wear respiratory protection. Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

13 Revision date: Pepsin Solution Product code: ES-0001 Page 4 of 8 SECTION 9: Physical and chemical properties 9.1. Information on basic physical and chemical properties Physical state: Colour: Odour: ph-value: Changes in the physical state Melting point: Initial boiling point and boiling range: Flash point: Flammability Solid: Gas: Lower explosion limits: Upper explosion limits: Auto-ignition temperature Solid: Gas: Decomposition temperature: Oxidizing properties Not oxidizing. Vapour pressure: Density: Water solubility: Solubility in other solvents Partition coefficient: Vapour density: Evaporation rate: 9.2. Other information Solid content: liquid colourless odourless insoluble Test method SECTION 10: Stability and reactivity Reactivity No hazardous reaction when handled and stored according to provisions Chemical stability The product is stable under storage at normal ambient temperatures Possibility of hazardous reactions No known hazardous reactions Conditions to avoid none Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

14 Pepsin Solution Revision date: Product code: ES-0001 Page 5 of Incompatible materials Hazardous decomposition products No known hazardous decomposition products. SECTION 11: Toxicological information Information on toxicological effects Acute toxicity CAS No Chemical name Exposure route Dose Species Source Method methanol oral dermal TRIS LD mg/kg LD mg/kg Rat Rabbit inhalative vapour LC50 85,3 mg/l Rat inhalative aerosol ATE 0,5 mg/l oral LD50 >5000 mg/kg Rat ECHA dermal inhalative LD50 >5000 mg/kg Data lacking Rat ECHA Irritation and corrosivity Sensitising effects Carcinogenic/mutagenic/toxic effects for reproduction STOT-single exposure STOT-repeated exposure Aspiration hazard Additional information on tests The mixture is classified as not hazardous according to regulation (EC) No 1272/2008 [CLP]. SECTION 12: Ecological information Toxicity The product is not: Ecotoxic. Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

15 Revision date: Pepsin Solution Product code: ES-0001 Page 6 of 8 CAS No Chemical name Aquatic toxicity Dose [h] [d] Species Source methanol Acute fish toxicity LC h fish ECHA mg/l Acute algae toxicity ErC mg/l Algae ECHA Acute crustacea toxicity EC h fish ECHA mg/l Fish toxicity NOEC 23,75 mg/l TRIS Acute algae toxicity ErC mg/l Algae 90 d fish ECHA ECHA Acute crustacea toxicity EC50 > h aquatic invertebrates ECHA mg/l Acute bacteria toxicity (>1.000 mg/l) 3 h microorganisms ECHA Method Persistence and degradability This information is not available. CAS No Chemical name Method Value d Evaluation TRIS DOC reduction. 97,1 % 28 Source Bioaccumulative potential The product has not been tested. Partition coefficient n-octanol/water CAS No Chemical name Log Pow methanol -0, Mobility in soil The product has not been tested Results of PBT and vpvb assessment The product has not been tested Other adverse effects Further information Do not allow to enter into surface water or drains. Do not allow to enter into soil/subsoil. SECTION 13: Disposal considerations Waste treatment methods Advice on disposal Do not allow to enter into surface water or drains. Do not allow to enter into soil/subsoil. Dispose of waste according to applicable legislation. Contaminated packaging Non-contaminated packages may be recycled. Handle contaminated packages in the same way as the substance itself. SECTION 14: Transport information Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

16 Revision date: Pepsin Solution Product code: ES-0001 Page 7 of 8 Land transport (ADR/RID) UN number: UN proper shipping name: Other applicable information (land transport) No dangerous good in sense of these transport regulations. Inland waterways transport (ADN) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Other applicable information (inland waterways transport) No dangerous good in sense of these transport regulations. Marine transport (IMDG) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Air transport (ICAO-TI/IATA-DGR) UN number: UN proper shipping name: Transport hazard class(es): Packing group: Environmental hazards ENVIRONMENTALLY HAZARDOUS: Special precautions for user Transport in bulk according to Annex II of Marpol and the IBC Code SECTION 15: Regulatory information - no Safety, health and environmental regulations/legislation specific for the substance or mixture National regulatory information Water contaminating class (D): Skin resorption/sensitization: Chemical safety assessment 2 - water contaminating Causes allergic hypersensitivity reactions. Chemical safety assessments for substances in this mixture were not carried out. SECTION 16: Other information Changes This data sheet contains changes from the previous version in section(s): 2,3,4,5,6,7,8,9,10,11,12,13,14,15,16. Abbreviations and acronyms ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage of Dangerous Goods by Road ) IMDG: International Maritime Code for Dangerous Goods IATA: International Air Transport Association Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

17 Revision date: Pepsin Solution Product code: ES-0001 Page 8 of 8 GHS: Globally Harmonized System of Classification and Labelling of Chemicals EINECS: European Inventory of Existing Commercial Chemical Substances ELINCS: European List of Notified Chemical Substances CAS: Chemical Abstracts Service LC50: Lethal concentration, 50% LD50: Lethal dose, 50% Relevant H and EUH statements (number and full text) H225 Highly flammable liquid and vapour. H301 Toxic if swallowed. H311 Toxic in contact with skin. H315 Causes skin irritation. H319 Causes serious eye irritation. H331 Toxic if inhaled. H335 May cause respiratory irritation. H370 Causes damage to organs. EUH208 Contains Pepsin A. May produce an allergic reaction. EUH210 Safety data sheet available on request. Further Information The information is based on present level of our knowledge. It does not, however, give assurances of product properties and establishes no contract legal rights. The receiver of our product is singulary responsible for adhering to existing laws and regulations. The above information describes exclusively the safety requirements of the product and is based on our present-day knowledge. The information is intended to give you advice about the safe handling of the product named in this safety data sheet, for storage, processing, transport and disposal. The information cannot be transferred to other products. In the case of mixing the product with other products or in the case of processing, the information on this safety data sheet is not necessarily valid for the new made-up material. (The data for the hazardous ingredients were taken respectively from the last version of the sub-contractor's safety data sheet.) Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

18 Revision date: Wash Buffer SSC Product code: WB-0001 Page 1 of 7 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Wash Buffer SSC 1.2. Relevant identified uses of the substance or mixture and uses advised against Use of the substance/mixture The product is intended for professional use Details of the supplier of the safety data sheet Company name: Street: Fischkai 1 Place: D Bremerhaven Telephone: +49 (0) 471/ Telefax:+49 (0) 471/ info@zytovision.com Internet: Emergency telephone number: SECTION 2: Hazards identification 2.1. Classification of the substance or mixture +49 (0) 471/ am - 5pm (CET) Regulation (EC) No. 1272/2008 Hazard categories: Respiratory or skin sensitisation: Skin Sens. 1 Hazard Statements: May cause an allergic skin reaction Label elements Regulation (EC) No. 1272/2008 Hazard components for labelling Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) Signal word: Pictograms: Warning Hazard statements H317 May cause an allergic skin reaction. Precautionary statements P261 Avoid breathing dust/fume/gas/mist/vapours/spray. P272 Contaminated work clothing should not be allowed out of the workplace. P280 Wear protective gloves/protective clothing/eye protection/face protection. P302+P352 IF ON SKIN: Wash with plenty of water. P333+P313 If skin irritation or rash occurs: Get medical advice/attention. P362+P364 Take off contaminated clothing and wash it before reuse Other hazards SECTION 3: Composition/information on ingredients 3.2. Mixtures Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

19 Wash Buffer SSC Revision date: Product code: WB-0001 Page 2 of 7 Hazardous components CAS No Chemical name EC No Index No REACH No Classification according to Regulation (EC) No. 1272/2008 [CLP] Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) Acute Tox. 3, Acute Tox. 3, Acute Tox. 3, Skin Corr. 1B, Skin Sens. 1, Aquatic Acute 1, Aquatic Chronic 1; H331 H311 H301 H314 H317 H400 H410 Full text of H and EUH statements: see section 16. Quantity < 20 % SECTION 4: First aid measures 4.1. Description of first aid measures After inhalation Provide fresh air. When in doubt or if symptoms are observed, get medical advice. After contact with skin After contact with skin, wash immediately with polyethylene glycol, followed by plenty of water. Take off immediately all contaminated clothing and wash it before reuse. Medical treatment necessary. After contact with eyes Rinse immediately carefully and thoroughly with eye-bath or water. In case of eye irritation consult an ophthalmologist. After ingestion Rinse mouth immediately and drink plenty of water Most important symptoms and effects, both acute and delayed 4.3. Indication of any immediate medical attention and special treatment needed Treat symptomatically. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Co-ordinate fire-fighting measures to the fire surroundings Special hazards arising from the substance or mixture Non-flammable Advice for firefighters Wear a self-contained breathing apparatus and chemical protective clothing. Full protection suit. Additional information Suppress gases/vapours/mists with water spray jet. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Provide adequate ventilation. Do not breathe gas/fumes/vapour/spray. Avoid contact with skin, eyes and clothes. Use personal protection equipment Environmental precautions No special environmental measures are necessary. Clean contaminated articles and floor according to the environmental legislation. Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

20 Revision date: Wash Buffer SSC Product code: WB-0001 Page 3 of Methods and material for containment and cleaning up Absorb with liquid-binding material (e.g. sand, diatomaceous earth, acid- or universal binding agents). Treat the recovered material as prescribed in the section on waste disposal Reference to other sections Safe handling: see section 7 Personal protection equipment: see section 8 Disposal: see section 13 SECTION 7: Handling and storage 7.1. Precautions for safe handling Advice on protection against fire and explosion No special fire protection measures are necessary Conditions for safe storage, including any incompatibilities Requirements for storage rooms and vessels Keep container tightly closed. Advice on storage compatibility No special measures are necessary. Further information on storage conditions Store, tightly closed, in the original vessel at conditions indicated on the label Specific end use(s) The product is intended for professional use. SECTION 8: Exposure controls/personal protection 8.1. Control parameters 8.2. Exposure controls Protective and hygiene measures Remove contaminated, saturated clothing immediately. Draw up and observe skin protection programme. Wash hands and face before breaks and after work and take a shower if necessary. When using do not eat or drink. Eye/face protection Wear eye/face protection. Hand protection When handling with chemical substances, protective gloves must be worn with the CE-label including the four control digits. The quality of the protective gloves resistant to chemicals must be chosen as a function of the specific working place concentration and quantity of hazardous substances. For special purposes, it is recommended to check the resistance to chemicals of the protective gloves mentioned above together with the supplier of these gloves. Skin protection Wear suitable protective clothing. Respiratory protection In case of inadequate ventilation wear respiratory protection. SECTION 9: Physical and chemical properties 9.1. Information on basic physical and chemical properties Physical state: Colour: Odour: liquid colourless odourless Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

21 Revision date: Wash Buffer SSC Product code: WB-0001 Page 4 of 7 ph-value: Changes in the physical state Melting point: Initial boiling point and boiling range: Flash point: Flammability Solid: Gas: Explosive properties No data available. Lower explosion limits: Upper explosion limits: Auto-ignition temperature Solid: Gas: Decomposition temperature: Oxidizing properties Not oxidizing. Vapour pressure: Density: Water solubility: Solubility in other solvents Partition coefficient: Vapour density: Evaporation rate: 9.2. Other information Solid content: easily soluble Test method SECTION 10: Stability and reactivity Reactivity No hazardous reaction when handled and stored according to provisions Chemical stability The product is stable under storage at normal ambient temperatures Possibility of hazardous reactions No known hazardous reactions Conditions to avoid none Incompatible materials Hazardous decomposition products No known hazardous decomposition products. Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

22 Wash Buffer SSC Revision date: Product code: WB-0001 Page 5 of 7 SECTION 11: Toxicological information Information on toxicological effects Acute toxicity CAS No Chemical name Exposure route Dose Species Source Method Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) oral dermal ATE 100 mg/kg ATE 300 mg/kg inhalative vapour ATE 3 mg/l inhalative aerosol ATE 0,5 mg/l Irritation and corrosivity Sensitising effects May cause an allergic skin reaction. Carcinogenic/mutagenic/toxic effects for reproduction STOT-single exposure STOT-repeated exposure Aspiration hazard Additional information on tests The mixture is classified as hazardous according to regulation (EC) No 1272/2008 [CLP]. SECTION 12: Ecological information Toxicity The product is not: Ecotoxic Persistence and degradability The product has not been tested Bioaccumulative potential The product has not been tested Mobility in soil The product has not been tested Results of PBT and vpvb assessment The product has not been tested Other adverse effects Further information Avoid release to the environment. SECTION 13: Disposal considerations Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

23 Revision date: Wash Buffer SSC Product code: WB-0001 Page 6 of Waste treatment methods Advice on disposal Do not allow to enter into surface water or drains. Dispose of waste according to applicable legislation. Contaminated packaging Wash with plenty of water. Completely emptied packages can be recycled. SECTION 14: Transport information Land transport (ADR/RID) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Other applicable information (land transport) No dangerous good in sense of these transport regulations. Inland waterways transport (ADN) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Other applicable information (inland waterways transport) No dangerous good in sense of these transport regulations. Marine transport (IMDG) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Air transport (ICAO-TI/IATA-DGR) UN number: UN proper shipping name: Transport hazard class(es): Packing group: Environmental hazards ENVIRONMENTALLY HAZARDOUS: Special precautions for user Transport in bulk according to Annex II of Marpol and the IBC Code SECTION 15: Regulatory information - no Safety, health and environmental regulations/legislation specific for the substance or mixture National regulatory information Employment restrictions: Water contaminating class (D): Skin resorption/sensitization: Observe restrictions to employment for juvenils according to the 'juvenile work protection guideline' (94/33/EC). - - not water contaminating Causes allergic hypersensitivity reactions. Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

24 Revision date: Wash Buffer SSC Product code: WB-0001 Page 7 of Chemical safety assessment Chemical safety assessments for substances in this mixture were not carried out. SECTION 16: Other information Changes This data sheet contains changes from the previous version in section(s): 2,3,4,5,6,7,8,9,10,11,12,13,14,15,16. Abbreviations and acronyms ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage of Dangerous Goods by Road ) IMDG: International Maritime Code for Dangerous Goods IATA: International Air Transport Association GHS: Globally Harmonized System of Classification and Labelling of Chemicals EINECS: European Inventory of Existing Commercial Chemical Substances ELINCS: European List of Notified Chemical Substances CAS: Chemical Abstracts Service LC50: Lethal concentration, 50% LD50: Lethal dose, 50% Relevant H and EUH statements (number and full text) H301 Toxic if swallowed. H311 Toxic in contact with skin. H314 Causes severe skin burns and eye damage. H317 May cause an allergic skin reaction. H331 Toxic if inhaled. H400 Very toxic to aquatic life. H410 Very toxic to aquatic life with long lasting effects. Further Information The information is based on present level of our knowledge. It does not, however, give assurances of product properties and establishes no contract legal rights. The receiver of our product is singulary responsible for adhering to existing laws and regulations. The above information describes exclusively the safety requirements of the product and is based on our present-day knowledge. The information is intended to give you advice about the safe handling of the product named in this safety data sheet, for storage, processing, transport and disposal. The information cannot be transferred to other products. In the case of mixing the product with other products or in the case of processing, the information on this safety data sheet is not necessarily valid for the new made-up material. (The data for the hazardous ingredients were taken respectively from the last version of the sub-contractor's safety data sheet.) Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

25 Revision date: Wash Buffer A Product code: WB-0002 Page 1 of 7 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Wash Buffer A 1.2. Relevant identified uses of the substance or mixture and uses advised against Use of the substance/mixture The product is intended for professional use Details of the supplier of the safety data sheet Company name: Street: Fischkai 1 Place: D Bremerhaven Telephone: +49 (0) 471/ Telefax:+49 (0) 471/ info@zytovision.com Internet: Emergency telephone number: SECTION 2: Hazards identification 2.1. Classification of the substance or mixture +49 (0) 471/ am - 5pm (CET) Regulation (EC) No. 1272/2008 Hazard categories: Respiratory or skin sensitisation: Skin Sens. 1 Hazard Statements: May cause an allergic skin reaction Label elements Regulation (EC) No. 1272/2008 Hazard components for labelling Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) Signal word: Pictograms: Warning Hazard statements H317 May cause an allergic skin reaction. Precautionary statements P261 Avoid breathing dust/fume/gas/mist/vapours/spray. P272 Contaminated work clothing should not be allowed out of the workplace. P280 Wear protective gloves/protective clothing/eye protection/face protection. P302+P352 IF ON SKIN: Wash with plenty of water. P333+P313 If skin irritation or rash occurs: Get medical advice/attention. P362+P364 Take off contaminated clothing and wash it before reuse Other hazards SECTION 3: Composition/information on ingredients 3.2. Mixtures Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

26 Wash Buffer A Revision date: Product code: WB-0002 Page 2 of 7 Hazardous components CAS No Chemical name EC No Index No REACH No Classification according to Regulation (EC) No. 1272/2008 [CLP] Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) Acute Tox. 3, Acute Tox. 3, Acute Tox. 3, Skin Corr. 1B, Skin Sens. 1, Aquatic Acute 1, Aquatic Chronic 1; H331 H311 H301 H314 H317 H400 H410 Full text of H and EUH statements: see section 16. Quantity < 20 % SECTION 4: First aid measures 4.1. Description of first aid measures After inhalation Provide fresh air. When in doubt or if symptoms are observed, get medical advice. After contact with skin After contact with skin, wash immediately with polyethylene glycol, followed by plenty of water. Take off immediately all contaminated clothing and wash it before reuse. Medical treatment necessary. After contact with eyes Rinse immediately carefully and thoroughly with eye-bath or water. In case of eye irritation consult an ophthalmologist. After ingestion Rinse mouth immediately and drink plenty of water Most important symptoms and effects, both acute and delayed 4.3. Indication of any immediate medical attention and special treatment needed Treat symptomatically. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Co-ordinate fire-fighting measures to the fire surroundings Special hazards arising from the substance or mixture Non-flammable Advice for firefighters Wear a self-contained breathing apparatus and chemical protective clothing. Full protection suit. Additional information Suppress gases/vapours/mists with water spray jet. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Provide adequate ventilation. Do not breathe gas/fumes/vapour/spray. Avoid contact with skin, eyes and clothes. Use personal protection equipment Environmental precautions No special environmental measures are necessary. Clean contaminated articles and floor according to the environmental legislation. Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

27 Revision date: Wash Buffer A Product code: WB-0002 Page 3 of Methods and material for containment and cleaning up Absorb with liquid-binding material (e.g. sand, diatomaceous earth, acid- or universal binding agents). Treat the recovered material as prescribed in the section on waste disposal Reference to other sections Safe handling: see section 7 Personal protection equipment: see section 8 Disposal: see section 13 SECTION 7: Handling and storage 7.1. Precautions for safe handling Advice on safe handling No special measures are necessary. Advice on protection against fire and explosion No special fire protection measures are necessary Conditions for safe storage, including any incompatibilities Requirements for storage rooms and vessels Keep container tightly closed. Advice on storage compatibility No special measures are necessary. Further information on storage conditions Store, tightly closed, in the original vessel at conditions indicated on the label Specific end use(s) The product is intended for professional use. SECTION 8: Exposure controls/personal protection 8.1. Control parameters 8.2. Exposure controls Protective and hygiene measures Remove contaminated, saturated clothing immediately. Draw up and observe skin protection programme. Wash hands and face before breaks and after work and take a shower if necessary. When using do not eat or drink. Eye/face protection Wear eye/face protection. Hand protection When handling with chemical substances, protective gloves must be worn with the CE-label including the four control digits. The quality of the protective gloves resistant to chemicals must be chosen as a function of the specific working place concentration and quantity of hazardous substances. For special purposes, it is recommended to check the resistance to chemicals of the protective gloves mentioned above together with the supplier of these gloves. Skin protection Wear suitable protective clothing. Respiratory protection In case of inadequate ventilation wear respiratory protection. Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

28 Revision date: Wash Buffer A Product code: WB-0002 Page 4 of 7 SECTION 9: Physical and chemical properties 9.1. Information on basic physical and chemical properties Physical state: Colour: Odour: ph-value: Changes in the physical state Melting point: Initial boiling point and boiling range: Flash point: Sustaining combustion: Flammability Solid: Gas: Explosive properties No data available. Lower explosion limits: Upper explosion limits: Auto-ignition temperature Solid: Gas: Decomposition temperature: Oxidizing properties Not oxidizing. Vapour pressure: Density: Water solubility: Solubility in other solvents Partition coefficient: Vapour density: Evaporation rate: 9.2. Other information Solid content: liquid colourless odourless No data available easily soluble Test method SECTION 10: Stability and reactivity Reactivity No hazardous reaction when handled and stored according to provisions Chemical stability The product is stable under storage at normal ambient temperatures Possibility of hazardous reactions No known hazardous reactions. Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

29 Wash Buffer A Revision date: Product code: WB-0002 Page 5 of Conditions to avoid none Incompatible materials Hazardous decomposition products No known hazardous decomposition products. SECTION 11: Toxicological information Information on toxicological effects Acute toxicity CAS No Chemical name Exposure route Dose Species Source Method Mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) oral dermal ATE 100 mg/kg ATE 300 mg/kg inhalative vapour ATE 3 mg/l inhalative aerosol ATE 0,5 mg/l Irritation and corrosivity Sensitising effects May cause an allergic skin reaction. Carcinogenic/mutagenic/toxic effects for reproduction STOT-single exposure STOT-repeated exposure Aspiration hazard Additional information on tests The mixture is classified as hazardous according to regulation (EC) No 1272/2008 [CLP]. SECTION 12: Ecological information Toxicity The product is not: Ecotoxic Persistence and degradability The product has not been tested Bioaccumulative potential The product has not been tested Mobility in soil The product has not been tested Results of PBT and vpvb assessment The product has not been tested. Revision No: 10,1 - Replaces version: 6 GB - EN Print date:

30 Wash Buffer A Revision date: Product code: WB-0002 Page 6 of Other adverse effects Further information Avoid release to the environment. SECTION 13: Disposal considerations Waste treatment methods Advice on disposal Do not allow to enter into surface water or drains. Dispose of waste according to applicable legislation. Contaminated packaging Wash with plenty of water. Completely emptied packages can be recycled. SECTION 14: Transport information Land transport (ADR/RID) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Other applicable information (land transport) No dangerous good in sense of these transport regulations. Inland waterways transport (ADN) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Other applicable information (inland waterways transport) No dangerous good in sense of these transport regulations. Marine transport (IMDG) UN number: UN proper shipping name: Transport hazard class(es): Packing group: - Air transport (ICAO-TI/IATA-DGR) UN number: UN proper shipping name: Transport hazard class(es): Packing group: Environmental hazards ENVIRONMENTALLY HAZARDOUS: Special precautions for user Transport in bulk according to Annex II of Marpol and the IBC Code SECTION 15: Regulatory information - no Revision No: 10,1 - Replaces version: 6 GB - EN Print date: