SYRINGE FILTERS. New Filter Housing Design. Phenex. Old. New. For HPLC and GC sample and solvent filtration. Improved Performance Benefits:

Transcription

1 Phenex SYRNGE FLTERS For HPLC and GC sample and solvent filtration Phenex Syringe Filters are now even better! With our quality and performance guarantees No change in membrane filtering characteristics No method revalidation necessary in most cases What else is better? Our quality control testing is even more rigorous All Syringe Filters are clean-room assembled Phenex Syringe Filters are convenient, economical, and dependable. Sample or Mobile Filter Membrane Hold-up Volume Phase Volume (ml) (diameter, mm) Format (µl) after Air Purge < 1 4 Syringe filter < 20 1 to Syringe filter < to Syringe filter < 140 > Membrane disk > Membrane disk New Filter Housing Design Old New 25 mm DA. 30 mm DA. 13 mm DA. 17 mm DA. mproved Performance Benefits: Feature Benefit Larger diameter ncreased sample membranes throughput Lower Easier filtering backpressures Added sample mproved membrane distribution rings flows Phenex Syringe Filters Filter Membrane Selection Guide Membrane Type Application Cellulose Acetate Very low protein retention. With glass pre-filter, this membrane is excellent for filtration of tissue culture media, general biological sample filtration and clarification. Not resistant to most solvents. Polypropylene (PP) Hydrophilic polypropylene membranes are good for filtration of nearly all HPLC and GC samples. The membrane is highly solvent resistant, and can withstand most aggressive chemicals. Highly suited for filtration of aqueous samples. Low protein binding gives very high recoveries (92 %) compared with nylon membranes (69 %). HPLC with UV detection shows these membranes have been optimized to ensure minimal extractables and false peaks. Nylon For general sample and solvent filtration. Nylon has inherent hydrophilic characteristics and works well with all aqueous- as well as most solvent-based samples. Nylon is excellent for most HPLC and GC sample and solvent preparations. Polyethersulfone (PES) and Both membranes exhibit very low protein binding. Both can be used for general biological sample filtration and sterilization. Polyvinylidene Difluoride Polyvinylidene Difluoride (PVDF) is especially useful in HPLC sample preparation and is highly resistant to most solvents. Polyethersulfone has very limited resistance to solvents (PVDF) and is generally used for aqueous-based biological samples. Both membranes exhibit good flow rate characteristics. Polytetrafluoroethylene An inherently hydrophobic membrane that is good for filtration of organic-based, highly acidic or basic samples and solvents. Widely used for (PTFE, Teflon ) chromatography, and for clarification of non-aqueous samples. Although this membrane is hydrophobic, it can be made hydrophilic by wetting the membrane with alcohol and then flushing with deionized water. ACCESSORES See p. 361 for gas-tight syringes with Teflon Luer lock tips. See p. 322 for Membrane Filters and Mobile Phase Filtration System. 320 Phenomenex

2 Why Filter? t s a great way to improve your results and protect your column ncrease column lifetime (saves money!) Ensure more accurate, consistent results Eliminate damaging microparticulates Minimize downtime and equipment replacement Phenex Syringe Filters offer many great features and benefits: Rapid filtration of HPLC, GC samples prior to analysis Nylon for both aqueous- and solvent-based samples PTFE for organic or highly acidic or basic samples Particulates can damage expensive equipment, valves, columns and pumps. They can also lead to erratic analytical results. Prefiltering samples prior to analysis is critical in preventing column and frit blockage, undue wear on valve seals, and abnormally high operating pressures. Moreover, sample and solvent filtration will lead to an overall improvement in baseline, quantitation, reproducibility, and detection limits. Phenex syringe filters are designed for the economical, rapid filtration of almost any solution prior to analysis. The housing attaches to any standard Luer hub syringe, so the sample can be pushed through the membrane under pressure. The resulting eluent is free from particulates and ready for use with HPLC, GC or other analytical techniques. Phenex syringe filters are available in a broad range of pore sizes and membrane types. All are non-sterile. Filters made with 17 or 30 mm membranes are suitable for use with samples of 1 ml or greater. Filters with 4 mm filter elements allow excellent recovery of samples less than 1 ml. Four and 17 mm filters are supplied with Nylon 66, polypropylene or PTFE membranes. 30 mm filters are available with polyethersulfone, cellulose acetate, PTFE, PVDF, polypropylene or Nylon 66 membranes. (Note: Both polypropylene and Nylon 66 are hydrophilic, will readily filter aqueous, organic, or aqueous/organic mixtures without prewetting, and contain no plasticizers.) PTFE is not recommended for the filtration of aqueous samples. Housings are available in polypropylene (PP) for acids and nonpolar solvents, and modified acrylic (MA) for aqueous samples. ORDERNG NFORMATON Phenex Syringe Filters Phenex Syringe Filters (cont'd) Pore Phenex Pore Phenex Part No. Size (μm) Membrane* Housing* Price Part No. Size (μm) Membrane* Housing* Price 30 mm Diameter 100/Box 17 mm Diameter 100/Box AF Cellulose Acetate MA AF Cellulose Acetate PP AF Nylon PP AF Nylon PP AF Nylon PP AF Nylon PP AF PTFE PP AF PTFE PP AF PTFE PP AF PTFE PP AF PES PP AF PES PP AF PVDF PP AF PVDF PP 30 mm Diameter 500/Box 17 mm Diameter 500/Box AF Nylon PP AF Nylon PP AF PTFE PP 4 mm Diameter 100/Box Puradisc Syringe Filters AF Polypropylene PP 13 mm Diameter 100/Box AF Nylon PP AF Polypropylene PP AF Nylon PP 25 mm Diameter 200/Box AF PTFE PP AF Polypropylene PP AF PTFE PP *MA = Modified Acrylic; PP = Polypropylene; PVDF = Polyvinylidene Difluoride; PTFE = Polytetrafluoroethylene; PES = Polyethersulfone. Puradisc is a trademark of Whatman. Whatman is a trademark of Whatman nternational Ltd. (1) Above syringe filters are non-sterile. Syringe filters with polypropylene housing and nylon or Teflon membranes (17 or 30 mm D only) can be autoclaved. Any filters with acrylic housing or any 4 mm D filter with polypropylene housing CANNOT be autoclaved. (2) For purchase of larger quantities at significant savings, please contact your Phenomenex representative. See p. 350 for the FilterSys Mobile Phase Filtration System. See p. 322 for Sample and Mobile Phase Filter Membranes ordering information. Phenex Syringe Filters ACCESSORES Phenomenex 321

3 FilterSys All-Glass Vacuum Mobile Phase Filtration System Prevents pump and system component damage Rapid filtration of buffers, organics and corrosive liquids Removes damaging microparticulates and bacterial contaminants HPLC and GC solvent and sample filtration, 1 to 4 L units FLTER MEMBRANES Nylon and PTFE (Teflon ) membranes Wide selection of membrane sizes Phenomenex PTFE (Teflon ) and Nylon membrane filters offer excellent chemical resistance to almost all laboratory solvents and samples. They do not introduce unwanted plasticizers or contaminants into the sample or mobile phase. PTFE (Teflon ) and Nylon 66 membrane disc filters are available in 13 and 25 mm diameters (for reusable syringe filter holders), and in 47 mm diameter for mobile phase filtration. Since Nylon 66 is hydrophilic, filtering of aqueous solvents is simple. No prewetting is required. PTFE is hydrophobic and so is not recommended for the filtration of aqueous solutions. ORDERNG NFORMATON Filter Membranes Pore Disc Membrane Part No. Size (μm) Diameter (mm) Material Unit Price Nylon AF Nylon 100/pk AF Nylon 100/pk AF Nylon 100/pk AF Nylon 100/pk AF Nylon 100/pk PTFE AF PTFE 100/pk AF PTFE 100/pk AF PTFE 100/pk FilterSys/Filter Membranes ACCESSORES ORDERNG NFORMATON Mobile Phase Filtration System Part No. Description Unit Price Complete Assembly AH FilterSys, 47 mm, 300 ml funnel w/1l vacuum flask ea AH FilterSys, 47 mm, 500 ml funnel w/2l vacuum flask ea AH FilterSys, 47 mm, 1000 ml funnel w/4l vacuum flask ea Component Parts AH Fritted support base, 47 mm, 40/35 taper ea AH Funnel, graduated, 300 ml, 47 mm ea AH Funnel, graduated, 500 ml, 47 mm ea AH Funnel, graduated, 1000 ml, 47 mm ea AH liter filter flask, 40/35 taper ea AH liter filter flask, 40/35 taper ea AH liter filter flask, 40/35 taper ea AH Aluminum clamp, 47 mm ea Filter Membranes AF Nylon 66, 0.2 µm, 47 mm 100/pk AF Nylon 66, 0.45 µm, 47 mm 100/pk AF PTFE, 0.2 µm, 47 mm 100/pk AF PTFE, 0.5 µm, 47 mm 100/pk solvent Pickup Adapter This glass adapter enables direct pickup of mobile phase solvent for filtration using the Phenomenex FilterSys. Safe in-line filtration with the pickup adapter replaces the tedious and dangerous pourand-wait funnel filtration method. Eliminate the possibilities of spilling solvents and breathing toxic vapors. Replacing the funnel adapter on the FilterSys unit, the 47 mm pickup adapter with flange holds the membrane filter in place on top of the fritted support base, which in turn is held by the aluminum clamp (not included). The pickup adapter draws solvent directly from the reagent bottle the safest way to transfer and filter solvents. The adapter includes a 4 mm PTFE stopcock with ¼" OD outlet, PTFE 90 elbow with compression fittings for ¼" OD tubing, and 3 feet of ¼" OD PTFE tubing. ORDERNG NFORMATON See p. 350 for additional information on the FilterSys Filtration System See p. 320 for a useful Membrane Selection Guide Solvent Pickup Adapter Part No. Description Unit Price AH Mobile Phase Pickup Adapter, 47 mm ea 322 Phenomenex

4 Centrifugal Filters SPNEX DSPOSABLE CENTRFUGAL FLTER UNTS Convenient filtration of multiple HPLC and GC samples High recovery for small samples Nylon and PTFE (Teflon ) membrane materials Filtration "Filtration is the easiest way to improve your results, guard your system components from damage, and protect your column investment" Regular sample filtration means: Less system downtime Fewer troubleshooting problems Removal of particulate matter to sub-micron levels is critical before any drug, tox, or dirty environmental sample is injected into an HPLC, GC or mass spectrometer. Also, products that remove matrix components, interferents, and chemical garbage will improve your results. Check out the following: Mini-ndex Page No. Spinex disposable centrifugal filter units provide a convenient means of filtering multiple HPLC and GC samples without the necessity of manually forcing each sample through the filter with a syringe. All you need is a laboratory centrifuge with sufficient capacity for the number of samples to be filtered at one time. Centrifugal force drives the sample through the filter quickly without effort on the part of the chemist. No cleaning of syringes is required between samples. Samples contact only the Spinex filter unit itself and the polypropylene receiver tube. After filtration the upper portion of the tube containing the membrane filter can be discarded. The receiver tube serves as a container for the filtered sample and can be retained as long as desired. Separate caps are supplied for the sample chamber and the receiver tube. Filter membrane materials available are Nylon 66 and PTFE (Teflon ). Nylon 66 Spinex filter units are recommended for most HPLC and GC sample filtration work. They are chemically resistant, compatible with all commonly used solvents, and virtually free of extractable materials that could contaminate samples. ORDERNG NFORMATON Centrifugal Filter Units Pore Volumes (ml) Membrane Part No. Size (μm) Sample/Receiver Non-Sterile Unit Price AF / 5.0 Nylon 25/pk AF / 5.0 Nylon 25/pk AF / 5.0 PTFE 25/pk AF / 5.0 PTFE 25/pk Filtration Products (General Laboratory) Syringe filters Membrane filters and filtration apparatus Centrifugal filters Solid Phase Extraction (SPE) devices Tubes Well plates Column Protection devices SecurityGuard Analytical (2 to 8 mm D) Semi-Prep (9 to 16 mm D) PREP (18 to 30 mm D) nlet filters Biocompatible PEEK Stainless steel n-line filters Biocompatible PEEK Analytical Stainless steel Analytical Prep Quality filtration and sample preparation products from Phenomenex offer a convenient, inexpensive way to clarify all your samples for peace of mind and improved analytical results. Centrifugal Filters ACCESSORES Phenomenex 323

5 Phenex Autosampler Vials 2.0 ml, 12 x 32 mm The Phenex line of vials and accessories is compatible with most autosamplers. Vials and inserts are made of borosilicate glass to exacting specifications; are heat treated to 1100 F to anneal the glass and burn off impurities before finally being packaged in a cleanroom environment. Ordering nformation Crimp Top, Wide-Mouth Vials AH AH Crimp seals - lacquered aluminum cap with large hole, for crimp top vial, containing the following septa: Rubber/PTFE septa AH Silicone/PTFE septa AH Screw Top, Standard Opening Vials AH AH Screw caps , polypropylene plastic for standard screw top vial Cap Only (without septa) AH Cap Only (without septa, wide lip) AH Septa for screw caps PTFE/Silicone AH PTFE/Silicone/PTFE septa AH PTFE (0.010 mm thick) AH Caps & Closures Phenex caps/closures are made to exacting specifications, giving a tight seal each and everytime. The aluminum crimp seals, plastic screw and snap caps come with highly pure septa material. All plastic caps come with a bonded-in septa that eliminates septum slipping into the vial. Ordering nformation Universal Vial Kits, Screw Top, Wide-Mouth, with Caps and Bonded-in Septa This universal vial can be used in any autosampler that utilizes a 12 x 32 mm vial. The 9 mm screw top style may be used in place of crimp top and snap ring top vials. Eliminates the need of stocking many different style vials. The top screws down in 1 /3 turn and eliminates the chore of crimping, de-crimping and snapping caps on. Caps come with bonded-in septa that eliminate septa slipping into vials. Vials and caps come in one convenient kit pack, and are available with or without pre-slit and/or write-on patch Description Type Part No. Price 1,000/pk Silicone/PTFE No-Slit AH Silicone/PTFE Pre-Slit AH Silicone/PTFE Pre-Slit, w/write-on Patch AH PTFE/Silicone/PTFE No-Slit AH Rubber/PTFE No-Slit AH Silicone/PTFE No-Slit, w/write-on Patch AH Silicone/PTFE Pre-Slit, w/write-on Patch AH PTFE/Silicone/PTFE No-Slit, w/write-on Patch AH Rubber/PTFE No-Slit, w/write-on Patch AH Phenex Autosampler Vials ACCESSORES Snap Ring Top, Wide-Mouth Vials AH AH Snap caps - for snap ring top vial, comes with bonded-in septa as follows: PTFE (most inert) AH Silicone/PTFE septa AH Limited Volume nserts Phenex limited volume inserts are made to exacting dimensional specifications to ensure consistent compatibility with our vials and are oven annealed at approximately 1100 F to burn off any impurities. Ordering nformation Vial nserts Wide-Mouth vial inserts - for the crimp top, snap ring top and screw top Wide-Mouth vials: 200 µl precision point TriSpring AH µl conical stand alone AH µl flat bottom AH Standard opening vial nserts - for screw top and standard opening vials: 100 µl precision point TriSpring AH µl conical stand alone AH µl flat bottom AH Plastic Vial Kits, Screw Top, Wide-Mouth, with Caps and Bonded-in Septa These 12 x 32 mm 300 µl disposable polypropylene vials feature a conical bottom for maximum sample recovery. Medical-grade polypropylene is chemically resistant and an excellent choice for ph-sensitive samples, sodium or heavy metal analysis. 9 mm caps come with bonded-in septa and screw down in 1 / 3 turn. An affordable and excellent alternative to glass vials, with everything you need in one convenient pack, these plastic vials are a great choice. Polypropylene Vial Kit Silicone/PTFE septa AH Silicone/PTFE septa with pre-slit AH Phenomenex

6 limited volume Vials ncrease sensitivity, selectivity and reproducibility by using a Phenomenex precision high recovery insert (previous page) or specialty vial. Such as these below. QsertVial This high quality microsampling device is one integral unit that consists of a 300 µl precision insert bonded into a 12 x 32 mm vial. Provides an absolute seal to the insert portion of the vial that eliminates evaporation from insert into vial. Eliminates contamination from handling of insert, assembly of insert into vial and saves time and money. The insert is precisely bonded into the vial assuring consistent centering and positioning of the vial in the autosampler for virtually error-free injections. For use with Agilent Technologies PerkinElmer Autosystem Spectra-Physics Varian Beckman Gilson Hitachi Waters' Alliance QsertVial kits, Clear wide-mouth vial, 12 x 32 mm, 300 µl volume, with write-on patch. Screw top with pre-assembled caps and septa ORDERNG NFORMATON QsertVial Part No. Description Price 100/pk AH QsertVial Kit, Silicone/PTFE septa AH QsertVial Kit, Silicone/PTFE septa with pre-slit headspace vials A clean vial with a cap that forms a tight seal is essential to achieving good results in headspace analysis. Quality headspace vials and caps at affordable prices are your guarantee from Phenomenex. Beveled tops with round or flat bottom styles are compatible with most instruments as indicated. Call us if you don t see what you need. Compatible with the following Autosamplers Round Bottom Flat Bottom Agilent HS 7694 X Carlo-Erba HS 850 X Dani HS / X Fisons HS 850 X PerkinElmer HS 100 / 101 / 40 X Tekmar-Dohrmann 7000HT X ThermoQuest HS 850, Trace GC HS 2000 X Varian Genesis X X Ordering nformation Headspace Autosampler Vials Part No. Description Price 100/pk AH Headspace Vial 20 ml clear, round bottom, 23 x 75 mm AH AH AH Headspace Vial 20 ml clear, flat bottom, 23 x 75 mm Aluminum Seal, with PTFE/Silicone septum, thickness Aluminum Seal, with Pharma-Fix molded pressure-release septum CD Vial This all-glass 1.5 ml maximum recovery vial has a precision-formed limited volume well in the center of a solid glass bottom that allows the autosampler to recover virtually the total sample. The wide-mouth opening eliminates bent needle problems. For use with Agilent Technologies PerkinElmer Autosystem Spectra-Physics Varian Beckman Gilson Hitachi Waters' Alliance CD Vial kits, Clear wide-mouth vial, 12 x 32 mm, 1.5 ml volume Screw top with pre-assembled caps and septa Autosampler Vials ORDERNG NFORMATON CD Vial Part No. Description Price 100/pk AH CD Vial Kit, Silicone/PTFE septa AH CD Vial Kit, Silicone/PTFE septa with pre-slit ACCESSORES Kit packs include caps. CD Vial and QsertVial are trademarks of AR Corporation. Round Bottom 20 ml Flat Bottom 20 ml Phenomenex 325