Figure 1: New gas chromatography system up and running in the RSF building at Hatfield Marine Science Center, Newport, OR Spring 2011.

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1 1. Research Equipment Reserve Fund (RERF) Spring 2011 Project Title: PI: Co-PI: Agilent/HP 6890 Gas Chromatograph Dr. Christopher Langdon Dr. Louise Copeman Date of Award: June, 2011 Award Amount: $26,688 from OSU Research Office $6,672 CIMRS match University Department: Cooperative Institute for Marine Resources Studies (CIMRS) Figure 1: New gas chromatography system up and running in the RSF building at Hatfield Marine Science Center, Newport, OR Spring 2011.

2 2. Final Budget Statement RERF funds were used to purchase a 7890A gas chromatography machine (Figure 1 & Figure 2A), a 7693A auto-injector (Figure 2B) and a 7693 sample tray (Figure 2B) from Agilent Technologies The CIMRS match funds were used to purchase the 7693A auto injector (Figure 2B), gas regulators (Figure 2C) as well as a service contract and various supplies for the GC instrument. RERF Expenditure Detail Funding Source Category Model No. Description $ Amount RERF Equipment G3440A 7890A Gas Chromatography 16, RERF Equipment G4513A 7693A Autoinjector 4, RERF Equipment G4514A 7693 Tray 5, TOTAL RERF 26, CIMRS- Equipment G4513A 7693A Autoinjector 1, Match CIMRS Match Supplies DB-WAX 30m, um (Column) CIMRS Match Maintenance SYS-GC- Advantage Bronze 1, C2 Gov't/Academia CIMRS Match Supplies 19199M Installation Kit for GCs w/out gas purifiers CIMRS Match Supplies Stage Regulator CIMRS Match Supplies Stage Regulator CIMRS Match Supplies Stage Regulator CIMRS Match Supplies HMT200-2 Trap, Hydrocarbon-H@), 1/8", cc CIMRS Match Supplies RMSH-2 Big Universal Trap, 1/8" ftgs, Helium CIMRS Match Supplies Gases \ Brackets CIMRS Match Supplies Misc. - TBD 1, TOTAL CIMRS MATCH 6, Partnership with COMES/OSU matching grant funds (Langdon-V0471B) allowed us to upgrade our original plans to purchase a refurbished 6890 GC model and purchase a new 7890A. This represents a value added use of RERF funding as the additional COMES-OSU money paid for new software, computer system, and application consulting. These services represent a $13,102 match for the research office and CIMRS. Items covered under the COMES-OSU funding were not eligible under the RERF funding guidelines.

3 A B c Figure 2: Gas Chromatography System in RSF-Room 132 at Hatfield Marine Science Center, A) GC 7890A, B) 7693A Auto injector & 7693 Tray.

4 3. A brief summary of the scholarly work/activities made possible as a result of the RERF funding. The acquisition of the GC system through the RERF award has allowed the initiation of a Marine Lipids Research Laboratory program at the Hatfield Marine Science Center. Lipids are an important biochemical component of marine food webs and are now routinely used as a biochemical tool to help understand predator-prey relationships, improve growth performance in aquaculture, and facilitate an understanding of larger scale oceanography processes. The GC system has been installed (Nov 28 th ) and Co-PI Copeman is working to calibrate the instrument and run standards. The first analyses of marine samples will begin in January The GC system has been entered into the fee for service program with OSU. An initial cost of $40 per fatty acids analyses factors in the cost of supplies and labor. This price may need to be adjusted in order to insure that the GC is financially secure: covered for supplies, replacement parts and trained technical staff. Below are some of the ongoing scholarly activities made possible by the location of the GC at HMSC-OSU: Project 1: Ryer et al. Juvenile Tanner Crab- is a CIMRS project studying the role of prey nutritional quality on growth of juvenile Tanner crabs from different nursery embayments around Alaska. Dr. Ryer (NOAA, Alaskan Fisheries) in collaboration with Dr. Copeman (CIMRS) have already collected and processed food web samples from the summer of 2010 and are currently processing The goal of this study is to use lipids and fatty acids to understand food web differences between habitat types. Funding approved for 2011 will involve processing lipid classes and fatty acids using the new RERF-funded GC. Project 2: Eckert et al. Red King Crab Aquaculture- is a collaborative project between Dr. Eckert (University of Alaska), Dr. Stoner (NOAA, Alaskan Fisheries) and Dr. Copeman (CIMRS). This aquaculture-enhancement project is focused on the early energetics of red king crab. Crab stocks have declined dramatically in the last 20 years, which was an impetus for developing culture techniques to use in stock enhancement. In order to improve crab growth and survival in hatcheries, studies are now underway to understand their early metabolism and nutritional requirements. In a new Alaska Sea Grant funded project we will complete fatty acid analyses on hatchery reared crabs using the RERF-funded GC. Project 3: Stubblefield et al. Effect of BP s Deep Horizon oil spill on marine invertebrate organisms OSU contribution. This is an industry funded project that Dr. Langdon (COMES- OSU) and Dr. Stubblefield (Toxicology-OSU) are leading in collaboration with British Petroleum. This project aims to determine the effects of NRDA oils (or surrogates), watersoluble fractions of lipophilic compounds and the dispersant Corexit 9500 on the American oyster Crassostrea virginica, quahog clam, as well as representative echinoderm and crustacean species. Current bioassays are underway at the HMSC and in the spring long-term toxicology tests will be undertaken to assess the impacts of exposure to oil on the gonadal lipid storage of various bivalve species. Samples are expect to be analyzed in the summer of 2012 using the RERF-funded GC

5 Project 4: Laurel et al. - Juvenile Pacific Cod- is a CIMRS project using fatty acid biomarkers to examine the sources of dietary carbon available to juvenile Pacific cod. Dr. Laurel (NOAA, Alaskan Fisheries) and Dr. Copeman (CIMRS) have collected cod in Alaska (2009 & 2010 & 2011) to compare the levels of terrestrial fatty acids in fish from offshore and nearshore habitats. The goals of this project are to 1) measure rates of uptake of fatty acids in cod tissues, 2) provide fisheries scientists with information on the importance of terrestrial sourced carbon to juvenile cod diets and finally, 3) to use fatty acid biomarkers to link juvenile fish with specific nursery habitats. Select samples from 2011 are currently being analyzed by Dr. Copeman to confirm trends seen in the previous years. 4. A brief summary of any additional scholarly activities the RERF funding made possible for the investigator(s). The acquisition of the equipment has supported the development of partnerships amongst OSU and NOAA researchers. The equipment was received in October and installed in late November. Numerous discussions have led to the strong possibility of several groups using the equipment during 2012 and onwards. However, the funding for these projects has not been finalized. See below for a list of pending projects: Project 1: Brodeur et al. Salmon Ecology- is a CIMRS project investigating foodweb dynamics in Columbia River Chinook and coho salmon. Dr. Brodeur (NOAA, Northwest), along with Dr. Ciannelli (COAS, OSU) and M. Litz (PhD Student, COAS,OSU) are investigating the nutritional processes that affect growth, condition and survival of juvenile salmon. Lipids and fatty acids will be used to characterize the differences in food quality between the California Current, Columbia River estuary, and Columbia River Plume ecosystems. Results will help salmon ecologist understand nutritional factors that impact salmon survival during their first summer at sea and may shed light on recent declines in local salmon stocks. Project 2: Peterson et al. Krill Feeding Ecology- is a CIMRS project lead by Dr. Peterson (NOAA, Northwest), Ms. Menkel (CIMRS), and Dr. Copeman (CIMRS). This project will investigate the energetics and feeding ecology of two species of krill commonly found off the coast of Oregon and California. Krill play a vital role in the food web of the Pacific Northwest as they transfer energy from phytoplankton to higher trophic levels, such as fish and sea birds. Despite their importance, little is known about the feeding ecology of krill or their energy dynamics related to reproduction and food availability. The goals of this project are to 1) compare the lipid dynamics of two co-occurring species 2) use fatty acid biomarkers to compare their diets and 3) to compare krill fatty acid markers between different years and variable oceanographic conditions. This information is vital to understanding how krill may respond to annual or larger scale climatic changes in phytoplankton production. Dr. Copeman and Dr. Peterson are developing ideas for a grant submission to NSF in 2012.

6 5. External funding requests that have been developed and submitted as a result of the RERF funding: NPRB: Arctic cod in a warming ocean: the interactive effects of temperature and food availability. $212,000. Submitted Dec 12, Co-PI Copeman (OSU-CIMRS) & Laurel (NOAA). Alaskan Sea Grant: Nutrition and condition of red king crab larvae: a case study for crustacean aquaculture in the US Western Region. $169,000. Funded with start date Spring PI Ginny Eckert (University of Alaska) & Collaborator Copeman (OSU-CIMRS). NOAA Essential Fish Habitat: Determinants of juvenile Tanner crab growth from different nursery embayments $87,000. Submitted in October, 2011 with funding to start in Spring PI Ryer et al. (NOAA) & Collaborator Copeman (OSU-CIMRS). Institutional Loan: Mark-V Iatroscan for Dr. Louise Copeman s efforts to develop a Marine Lipids Research Laboratory at CIMRS-OSU.~50,000 value. The Iatroscan will be used in combination with the GC purchased by the RERF award determine complimentary measure of lipid classes. The loan is for 3 years. Copeman (OSU-CIMRS) & Ryer (NOAA). Industrial Research Award British Petroleum: Effect of BP s Deep Horizon oil spill on marine invertebrate organisms. $2,030,905. Funding began in September PIs Stubblefield (OSU-Environmental and Molecular Toxicology) and Langdon (OSU-COMES).