HEALTHY FISH MEAN PROFITS

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1 HEALTHY FISH MEAN HEALTHY PROFITS

2 Reliable, inexpensive alternatives to laboratory testing The best way to keep fish healthy and protect your investment is to stay on top of water chemistry. Commercial testing laboratories can provide the necessary information to maintain water quality standards, but only at the expense of time and money. Now, Hach offers you a choice of easy-to-use, inexpensive alternatives. Hach s portable aquaculture test kits and laboratories provide fast results, when and where you need them. By detecting imbalances in water chemistry, you can prevent trouble before it happens. Whether you need a simple, economical kit for the analysis of specific parameters, or an advanced portable laboratory equipped with the versatile DR/2010 Spectrophotometer or DR/850 Colorimeter, Hach has a system designed for you. FF-1A Aquaculture testing capabilities Use the table below to determine which packaged Hach system best suits your testing needs. 2 Test FF-1A FF-2 FF-3 DREL/2010 CEL/850 Acidity Alkalinity Ammonia, for fresh water Ammonia, for salt water Carbon Dioxide Chloride Copper Dissolved Oxygen Hardness Iron Nitrate Nitrite ph Phosphorus TDS/Salinity Temperature FF-2 FF-3

3 Crop protection made easy as 1,2,3 Model FF-1A Aquaculture Test Kit Hach s popular FF-1A Aquaculture Test Kit is the perfect companion for the budget-conscious analyst. Ideal for onsite or laboratory analyses, the economical test kit includes the necessary apparatus and reagents for the measurement of nine critical parameters. The low-cost system uses the drop-count method for titration tests, and Hach color comparators for colorimetric tests. Colorimetric tests provide results directly in milligrams per liter (mg/l), and drop-count tests require, at most, a simple multiplication or division step to determine the concentration. Test Method/Chemistry Range or Readout (mg/l)* Alkalinity Drop count titration/sulfuric acid 0.4 to 8 gpg Ammonia, for fresh water Color disc/nessler reagent 0 to 3 Carbon Dioxide Drop count titration/sodium hydroxide 5 to 100 Chloride Drop count titration/silver nitrate 5 to 400 Dissolved Oxygen Drop count titration/modified Winkler 0.2 to 20 Hardness Drop count titration/edta 1 to 30 gpg Nitrite Color disc/diazotization 0 to 0.5 ph Color disc/wide range indicator 4 to 10 ph units Temperature 30 to 120 F How to Order Model FF-1A Aquaculture Test Kit. Includes reagents and apparatus for approximately 100 tests of each parameter. Model FF-2 Aquaculture Test Kit Hach s most complete freshwater aquaculture kit includes all apparatus and reagents needed to perform 10 critical colorimetric and titrimetric tests. With the convenience of concentrated titrant solutions in disposable cartridges, the FF-2 s easy-to-read Digital Titrator guarantees repeatability from test to test. In tests performed with a color omparator, the color of reacted sample in a vial is compared with a color standard calibrated in mg/l. The determination is made by visually matching the colors. Test Method/Chemistry Range or Readout (mg/l)* Acidity Digital Titrator/sodium hydroxide 100 to 4000 Alkalinity Digital Titrator/sulfuric acid 100 to 4000 Ammonia, for fresh water Color disc/nessler reagent 0 to 3 Carbon Dioxide Digital Titrator/sodium hydroxide 10 to 100 Chloride Digital Titrator/mercuric nitrate 10 to 160 Dissolved Oxygen Digital Titrator/modified Winkler 0 to 10 Hardness Digital Titrator/EDTA 100 to 4000 Nitrite Color disc/diazotization 0 to 0.5 ph Color disc/wide range indicator 4 to 10 ph units Temperature 30 to 120 F How to Order Model FF-2 Aquaculture Test Kit with Digital Titrator. Includes reagents and apparatus for approximately 100 tests of each parameter. Model FF-3 Saltwater Aquaculture Test Kit Now maintaining water quality in saltwater operations is easier than ever. Hach s Model FF-3 includes everything you will need to protect your crop against water chemistry imbalances. Measure salinity (without investing in an expensive refractometer), ammonia in salt water samples, and eight other critical parameters. Designed exclusively for the varied demands of specialized saltwater applications, the FF-3 also includes the durable Digital Titrator, detailed instructions and all necessary reagents and apparatus for testing. Test Method/Chemistry Range or Readout (mg/l)* Acidity Digital Titrator/sodium hydroxide 100 to 4000 Alkalinity Digital Titrator/sulfuric acid 100 to 4000 Ammonia, for salt water Color disc/salicylate 0 to 3 Carbon Dioxide Digital Titrator/sodium hydroxide 10 to 100 Chloride Digital Titrator/mercuric nitrate 2 to 100 ppt as salinity Dissolved Oxygen Digital Titrator/modified Winkler 0 to 10 Hardness Digital Titrator/EDTA 100 to 4000 Nitrite Color disc/diazotization 0 to 0.5 ph Color disc/wide range indicator 4 to 10 ph units Temperature 30 to 120 F How to Order Model FF-3 Saltwater Aquaculture Test Kit with Digital Titrator. Includes reagents and apparatus for approximately 100 tests of each parameter. *mg/l unless stated otherwise 1 gpg = 17 mg/l or 17 parts per million ppt = parts per thousand 3

4 DREL/2010 Portable Aquaculture Laboratory (Shown with optional 2100P Portable Turbidimeter) Customized testing anytime, anywhere With reagents, apparatus and analytical instrumentation to measure 13 critical parameters, the DREL/2010 Portable Aquaculture Laboratory is Hach s most complete portable aquaculture system. Suitable for fresh- or saltwater testing, the portable laboratory includes both the versatile DR/2010 Datalogging Spectrophotometer with test procedure prompting messages in five languages, and the Digital Titrator. Ideal for large scale operations and research work, the DREL/2010 also includes the EC10 ph Meter and Hach One Combination ph Electrode, Dissolved Oxygen (DO) Sample Apparatus and an illustrated procedures manual. Hach One and AccuVac are Hach Company trademarks. Test Method/Chemistry Range (mg/l)* Alkalinity Digital Titrator/sulfuric acid 10 to 1000 Ammonia, for saltwater DR/2010/salicylate 0 to 0.5 Carbon Dioxide Digital Titrator/sodium hydroxide, phenolphthalein 10 to 1000 Chloride Digital Titrator/mercuric nitrate 10 to 1000 Copper DR/2010/porphyrin 0 to 210 µg/l Dissolved Oxygen, Low Range Digital Titrator/modified Winkler 0 to 10 Dissolved Oxygen, Super High Range (AccuVac ) DR/2010/HRDO 0 to 45 Hardness Digital Titrator/EDTA 10 to 1000 Nitrite (AccuVac) DR/2010/diazotization 0 to 0.3 ph ph Meter 0 to 14 ph units Phosphorus, reactive (AccuVac) DR/2010/ascorbic acid 0 to 2.50 Salinity Digital Titrator/mercuric nitrate 0 to 100 ppt Temperature 30 to 120 F How to Order DREL/2010 Portable Aquaculture Laboratory. 4 *mg/l unless stated otherwise Reagents for tests marked (AccuVac) are available in vacuum-sealed AccuVac glass ampuls. See page 6 for description. ppt = parts per thousand

5 CEL/850 Portable Aquaculture Laboratory High-value, high-capability lab for easy field results The newest addition to our family of aquaculture testing products, the CEL/850 Aquaculture Laboratory, tests 13 parameters. The lab includes our new DR/850 Colorimeter, the EC10 ph/mv/temperature Meter with Hach One Electrode, the TDS Pocket Pal Tester, and the Digital Titrator. A detailed, illustrated procedures manual is also provided, allowing even inexperienced users to get fast, accurate results. Ideal for field or research testing, this laboratory can be easily expanded to include tests specific to your unique needs. The DR/850 Colorimeter is capable of testing over 50 parameters, so you can easily expand your testing program by simply ordering additional reagent sets. Test Method/Chemistry Range or Readout* Alkalinity (as CaCO 3 ) Digital Titrator/sulfuric acid 10 to 4000 Ammonia (as N) Colorimetric/salicylate 0 to 0.5 Carbon Dioxide Digital Titrator/sodium hydroxide, phenolphthalein 10 to 1000 Chloride Digital Titrator/silver nitrate 10 to,000 Dissolved Oxygen Colorimetric/HRDO 0 to 15 Hardness (as CaCO 3 ) Digital Titrator/EDTA 10 to 4000 Iron Colorimetric/phenanthroline 0 to 3.0 Nitrate Colorimetric/chromotropic acid 0 to 30 Nitrite (as N) Colorimetric/diazotization 0 to 3.5 ph EC10 ph Meter 2 to ph units Phosphate, reactive Colorimetric/ascorbic acid 0 to 2.5 TDS TDS Pocket Pal Tester 10 to 1990 Temperature EC10 ph Meter 5.0 to 105 C How to Order *mg/l unless stated otherwise CEL/50 Portable Aquaculture Laboratory 5

6 Analysts worldwide rely on Hach s proven methods Hach test kits and portable laboratories are designed to provide the accuracy and convenience today s analysts demand. Each system includes durable instruments, simplified methods, clearly written step-by-step instructions to guide you through each procedure, and prepared reagents that require no mixing or measuring. DR/2010 Spectrophotometer Included in the DREL/2010 Portable Laboratory, the DR/2010 Datalogging Spectrophotometer is rugged and compact enough to be a field instrument, yet accurate and stable enough to satisfy the most exacting analysts. Calibrations for over 120 commonly performed analyses are permanently stored in the instrument s read-only memory (ROM), or add up to 50 of your own calibrations. Extensive datalogging capabilities simplify field record-keeping, and user-friendly software and RS232 computer interface capibilities provide easy data management. Prompting messages can be selected to appear in any of five languages, including French, German, Spanish and Dutch. For more information, return the reply card to the Hach office or distributor serving you. DR/850 Colorimeter The key instrument in our CEL/850 Portable Aquaculture Laboratory, the DR/850 offers valuable features not commonly found in an affordable field instrument. The chemical-resistant, dustproof, waterproof DR/850 is designed to handle the rigors of field testing, yet provides advanced features such as datalogging of up to 50 measurements and the ability to accept five custom-generated procedures with up to 12 data points each. Operation is pushbutton simple, with a large, easy-to-read liquid crystal display that provides prompts to guide you through test procedures. Simply choose the program you want, prepare a blank and zero the instrument, then react the sample and read the results. For more information, check the appropriate box on the reply card. Drop Count Titration A low-cost, portable titration method, drop counting is done using standardized titrants in special dropper bottles with calibrated droppers. To titrate, add an indicator to the sample, then add titrant dropwise until the color changes. Accuracy is within ± 5 %. Results are proportional to the number of drops used. Titrimetric Hach s Digital Titrator is a unique concept in titrimetric analysis. A precision dispensing device fitted with concentrated titrants in compact interchangeable cartridges, the Digital Titrator guarantees repeatability from test to test, and consistency from person to person. Prepared titrant solutions eliminate the time and labor involved in preparation of reagents and clean-up of burets and glassware. Equally suited for both laboratory and field analysis, the unbreakable Digital Titrator comes with a sturdy carrying case and a complete instruction manual. Colorimetric With this method you add one or more reagents to the sample to produce a color proportional to the substance you want to measure. Hach s unique color comparator uses continuous gradient, calibrated color discs to eliminate time-consuming and inaccurate interpolation between individual color standards. Convenient Reagent Packaging Hach reagents are developed specifically for use in our compact, easy-to-use systems. If several reagents are needed in a single test, our chemists have often found ways to combine them in a single reagent package. Pillows Hach packages many reagents in convenient polyethylene or foil powder pillows. Pillow- or foil-packed reagents are protected from moisture and air, and are safer to handle. AccuVac Ampuls AccuVac optical quality glass ampuls are fast, easy to use, and eliminate sample cell clean-up. Packed under vacuum, each ampul contains the exact amount of reagent(s) for a single test. 6

7 Single parameter test kits Specifications and ordering information Single parameter test kits are available in a number of ranges and configurations. Use the table below to determine which kits will best suit your needs. Smallest Approx. Test Cat. No. Model Range* Increment* Method/Chemistry No. of Tests Acidity AC-DT 10 to to 1.0 Digital Titrator/sodium hydroxide 100 MO P Methyl-orange (MO) & Phenolphthalein (P or Total) As CaCO 3 Alkalinity AL-AP 0.4 to 8 gpg 0.4 gpg Drop count titration/sulfuric acid 100 P M Phenolphthalein (P) & Total (M) 1 to 20 gpg 1 gpg As CaCO AL-DT 10 to Digital Titrator/sulfuric acid 100 P M AL-B 0.4 to 8 gpg 0.4 gpg Drop count titration/sulfuric acid 100 P M 1 to 20 gpg 1 gpg Ammonia NI-8 0 to Color disc/nessler reagent 300 As NH 3 N For fresh water Carbon Dioxide CA to Drop count titration/ 200 As CO 2 2 to 40 2 sodium hydroxide, phenolphthalein 5 to CA-DT 10 to to 2.0 Digital Titrator/ 50 LR + 50 HR sodium hydroxide, phenolphthalein Chloride P 5 to Drop count titration/silver nitrate 100 As Cl 20 to CD-DT 10 to to 20 Digital Titrator/mercuric nitrate CD-B 5 to Drop count titration/silver nitrate to Copper LP Cube 0 to Long-path cube/porphyrin 25 As free Cu Dissolved Oxygen OX-2P 0.2 to Drop count titration/modified Winkler to AccuVac 0 to Color disc/hrdo OX-DT 1 to to 0.2 Digital Titrator/modified Winkler 50 Hardness, Total B 1 to 30 gpg 1 gpg Drop count titration/edta 100 As CaCO HA-DT 10 to to 10 Digital Titrator/EDTA 50 LR + 50 HR Nitrite NI-15 0 to Color disc/diazotization 100 As NO 2 N ph (Pocket Pal Tester) to 14 ph 0.1 ph Electrochemical 200 Hours Phosphorus PO-19A 0 to 5, 0 to , 1 Color disc/ascorbic acid AccuVac 0 to Color disc/ascorbic acid 25 Temperature (Pocket Pal Tester) to 70 C 0.1 C Thermistor 200 Hours *mg/l unless stated otherwise 1 gpg = 17 mg/l or 17 parts per million 7

8 Reliable testing at an economical price Pocket Colorimeter Though it fits into the palm of your hand, the battery-powered Pocket Colorimeter Instrument offers performance and capabilities rivaling larger, more expensive laboratory instruments. The lightweight, portable colorimeter provides direct digital readout in mg/l and features a durable long-lasting LED optical system designed to provide optimum performance test after test. Pocket Colorimeters are supplied with everything you need to begin testing including an illustrated procedures manual, premeasured reagents, testing apparatus and a durable carrying case. Approx. No. Description Cat. No. Range of Tests Ammonia to 0.50 mg/l to 12.5 mg/l by dilution Bromine to 10 mg/l 50 0 to 4.5 mg/l 100 Chlorine, Free to 4.5 (T) mg/l 50 (F) & Total (T) 0 to 2.00 (F)(T) mg/l 100 Chlorine & ph to 2.00 mg/l (Cl ) to 7.6 ph 100 Copper to 4.00 mg/l 100 Iron, TPTZ to 1.00 mg/l 50 Monochloramine/ to 0.45 mg/l as N 50 Free Ammonia (0 to 1.65 NH 2 Cl, 0 to 0.55 NH 3 ) Nitrate to 30 mg/l 100 Oxygen, Dissolved to 10 mg/l 50 Ozone to 0.25 mg/l 50 0 to 0.75 mg/l Phosphate to 3.00 mg/l 100 Pocket Turbidimeter The portable Pocket Turbidimeter Analysis System offers rugged construction and simple operation, and is ideal for quick spot checks in the range of 0 to 400 NTU. The instrument s 880 nm LED light source provides long life and excellent performance even with colored samples. Pocket Turbidimeter Analysis System EC10 Portable ph Meter The EC10 Portable ph/mv/ Temperature Meter is an affordable, easy-to-use instrument for field or lab ph measurement. Housed in an ergonomic, splash-resistant enclosure, the instrument offers an LCD digital readout and automatic buffer recognition. It comes with buffers, beakers, manuals and a choice of electrodes, in a rugged carrying case. EC10 ph Meter (with Hach One Combination ph Electrode with temperature) (with gel-filled combination ph electrode with temperature) 8

9 Additional equipment for water quality testing Complete your test kit or portable laboratory with time-saving meters, instruments, test kits, and accessories available from Hach. AccuVac Ampul Field Test Kits AccuVac Ampul field test kits provide fast, simple measurement and immediate, accurate results. Reagent is contained in a vacuum vial. Immersing the vial and snapping the tip draws in a measured sample, where it reacts to develop color. A color wheel provides color comparison with a sample blank and the reacted sample, with direct readout of DO. AccuVac DO Kit, High-Range (0-15 mg/l) AccuVac Dissolved Oxygen Sampler Also available is a sampler designed specifically for vacuum-sealed AccuVac Ampuls. If you are performing colorimetric dissolved oxygen measurement with AccuVac Ampuls, the new DO Sampler will save time and effort. When the sampler is immersed, the operator activates a pneumatic piston which snaps the AccuVac s prescored tip. The Ampul, which serves both as measuring vial and cuvette, fills automatically and is ready for measurement. AccuVac DO Sampler Dissolved Oxygen Meter The DO175 is a very capable instrument for lab or field measurement of dissolved oxygen from 0 to 20 mg/l. It comes with probe, electrolyte, beakers, manual and other accessories in a durable carrying case. The instrument offers easy calibration, RS232 output, and internal datalogging for simplified field work. DO175 Dissolved Oxygen Meter Dissolved Oxygen Sampler Dissolved oxygen profiles require that samples taken at various depths be free of interference by surface contaminants. The Hach sampler, complete with a 3-m (10') brass chain and 60-mL, glass-stoppered sample bottle is ideal. Constructed of PVC, it will not rust or corrode. Dissolved Oxygen Sampler

10 Comprehensive systems for analysis A world leader in simplified quantitative analysis, Hach offers a comprehensive line of packaged test systems for food and feed, soil, and microbiological analyses. These systems, developed by an expert staff of research scientists, are backed by step-by-step testing procedures and are designed to the most exacting specifications. Complete ordering information and descriptions of available products are detailed in our catalogs. To receive your free copies, simply return the inserted reply card to the Hach office or authorized distributor serving you. Test Kits Measure Everything from A to Z Hach offers over 200 different colorimetric and titrimetric procedures to measure everything from acidity to zinc. Each kit includes all necessary apparatus, step-by-step instructions and unit-dose reagents for consistent results. Soil Analysis Nutrients and contaminants contained in soil often have a dramatic influence on the water quality of fish and crab ponds. Because analytical methods will vary depending on the type of soil and the geographic location, it is important to choose the extractants, analytical reagents and methods most appropriate for your soil. Customized analytical testing systems from Hach will help you assess and correct environmental factors which influence production. Simply choose the system best suited to your needs. All feature ph and major nutrient and soil fertility determinations. Microbiological Analysis of Shellfish Microbiological analysis of shellfish is an important gauge of product quality. The presence of Escherichia coli (E. coli) indicates fecal pollution and suggests that other microorganisms of fecal origin, including many disease-producing organisms, may be present. Shellfish tested by APHA (American Public Health Association) methods, however, often produce a large number of false positives. As a result, thousands of dollars are lost for each day shellfish are unnecessarily quarantined. Hach has developed a simple method for the identification and enumeration of E. coli that is faster, less expensive and more sensitive than the APHA-sanctioned method. Use of 4-methylumbelliferyl-ß-D-glucuronide (MUG) reagent can produce savings for shellfish processors by eliminating unnecessary quarantines. 10

11 Monitor critical water quality tests The tests included in Hach s aquaculture kits and portable laboratories are also available individually. Choose single-parameter kits to measure the following if your testing needs are limited to specific parameters. Complete specifications and ordering information are detailed in the adjoining table. Acidity Acidity is usually caused by the presence of mineral acids, salts of strong acids or free carbon dioxide. Although organic acids of natural origin are usually not a problem, mineral acid pollution is detrimental to fish. Alkalinity Alkalinity buffers the water and prevents sudden changes in ph. Alkalinity levels of 100 to 120 mg/l with ph values between 7 and 8 generally are recognized as best for support of diversified aquatic life. Ammonia Ammonia can be toxic to fish at high ph and low dissolved oxygen levels. It decreases the ability of fish to take oxygen into the blood and can cause suffocation. Ammonia levels as low as 0.06 mg/l can damage gills, reduce feeding and impair natural functions. Carbon Dioxide Toxicity of carbon dioxide varies by fish type, water temperature and dissolved oxygen content. In some cases, carbon dioxide can have beneficial effects by lowering the ph and reducing ammonia toxicity. Stressful to fish, carbon dioxide hinders oxygen uptake and has a marked effect on fish behavior. Chloride Effects of chloride are closely related to total salinity and its effects on the ability of fish to take up oxygen. Freshwater fish cannot tolerate sharp changes in salinity. For some fish, 400 mg/l chloride can be harmful. Chloride can be beneficial where nitrite levels tend to be high. Often added to catfish ponds, chloride will control the uptake of nitrite by the fish. Copper Copper may exist in natural waters as a soluble salt or suspended solid. A small amount is essential for plants and animals, and concentrations exceeding 0.1 mg/l are useful for controlling algae and plankton growth. In saltwater aquaria, copper (in the form of copper sulfate) is often used as a treatment for parasite-infested fish. Though saltwater fish are tolerant to high levels of copper, the dosage should not exceed 0.25 mg/l. Dissolved Oxygen As the most critical parameter for a healthy fish environment, dissolved oxygen must be measured frequently. Oxygen requirements of the fish vary with the species and age of the fish, prior acclimatization, temperature and the concentrations of other substances in the water. Supersaturation also can be detrimental to fish production. Hardness Soft water increases the sensitivity of fish to toxic metals, so some hardness is beneficial. However, excess hardness in aquaria can limit fish growth. Nitrite Nitrites are toxic to fish and should be monitored closely, particularly when high-poundage production is an objective. Brown blood disease, a problem in catfish farming, is caused by toxic levels of nitrites which can occur when chloride and oxygen levels are too low. High nitrite levels also are detrimental to the oxygen supply because plankton growth is stimulated. ph ph controls the degree of dissociation of many substances. The optimum range for any particular kind of fish depends on temperature, dissolved oxygen, prior acclimatization and the content of various anions and cations. In most cases, a ph range of 6.5 to 8.2 is suitable. Phosphorus Phosphate enters water supplies from soil run-off, industrial operations, and sewage. Although necessary for biological growth, too much phosphate can cause excessive growth of algae, and may lead to eutrophication. Temperature High temperature can be detrimental to fish for a number of reasons. Not only is there a maximum temperature at which fish can live, but the solubility of oxygen in water, along with its availability to the fish, diminishes at high temperatures. Furthermore, the oxygen demand of fish and other aquatic life increases as the temperature rises. Sudden temperature changes, either up or down, are likely to be harmful. Turbidity This general characterization of water quality may be correlated to the performance of water filtration. Many fish species cannot tolerate high turbidity. 11

12 Analyze critical water quality parameters with Hach systems for fresh- and saltwater aquaculture testing Single parameter test kits Packaged, ready-to-use test systems Portable laboratories Fast E. coli detection Fish feed analysis Pond sludge testing

13 Please send the following literature: DR/2010 Datalogging Spectrophotometer English (3308) Spanish (3311) French (3310) German (3309) DR/800 Series Colorimeter (1903) Test Kits and Portable Laboratories English (4553) Spanish (4640) Portuguese (3727) French (3755) German (3888) Systems for Soil Analysis English (1868) Spanish (1876) French (1877) Systems for Microbiological Analysis English (3986) Spanish (3798) French (3799) German (3715) Free subscription to News & Notes Worldwide Update for the Analyst newsletter fold here I am interested in testing for Please have a technical representative call me at Name first last Title Department Company Address City Province/State Country Postal Code Type of Business Phone

14 NO POSTAGE NECESSARY IF MAILED IN THE UNITED STATES BUSINESS REPLY MAIL FIRST CLASS MAIL PERMIT NO 100 LOVELAND CO POSTAGE WILL BE PAID BY ADDRESSEE HACH COMPANY PO BOX 389 LOVELAND CO tape here DO NOT STAPLE