Table of Contents. High Resolution Melt Software [HRM] v3.0.1 Release Notes

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1 v3.0.1 Release Ntes Table f Cntents 1. OVERVIEW 2. FEATURES in HRM v SUSTAINED FEATURES 4. SYSTEM REQUIREMENTS 5. INSTALLATION OF THE THE SOFTWARE HRM V SUSTAINED KNOWN ISSUES 7. LEGAL STATEMENTS 1. OVERVIEW High reslutin melting (HRM) analysis is a new, pst-pcr analysis methd used fr identifying genetic variatin in nucleic acid sequences. Simple and fast, this methd is based n PCR melting (dissciatin) curve techniques and is enabled by the recent availability f imprved duble-stranded DNA (dsdna) binding dyes alng with next- generatin realtime PCR instrumentatin and analysis sftware. HRM analysis can discriminate DNA sequences based n their cmpsitin, length, GC cntent, r strand cmplementarities. HRM analysis differs frm standard melt curve analysis in three ways: 1. Chemistry HRM analysis uses brighter dyes at higher cncentratins. 2. Instruments HRM analysis requires instruments that cllect flurescence data at finer temperature reslutin. 3. Sftware HRM analysis requires mre sphisticated sftware which uses new flurescent scaling algrithms and plts. Applicatins fr HRM Sftware v3.0.1 include SNP detectin, Gentyping, Mutatin Screening, Methylatin analysis Rev A Cpyright 2011 Life Technlgies Crpratin. All Rights Reserved. Page 1 f 5

2 v3.0.1 Release Ntes 2. FEATURES in HRM v3.0.1 The HRM v3.0.1 Sftware cntains all features frm the previus versin 3.0 as well as the fllwing: New feature added a) HRM v3.0.1 Sftware nw supprts Windws 7. b) HRM v3.0.1 Sftware nw supprts the fllwing instrument files StepOne TM Sftware v2.2 r later [StepOne TM and StepOnePlus TM Real- Time PCR Systems] 7500 Fast Sftware v2.0.5 r later. Bug Fixes Fix fr knwn issue t allw installatin n system with 3GM f RAM size. 3. SUSTAINED FEATURES Plate Setup: Ability t Create, Save r Delete Samples, Targets, Cntrls and save them in a library. Easy plate setup that allws the user t assign r edit task, target, cntrl, sample infrmatin and experiment prperties in the HRM sftware fr analysis. Ability t stre the defined plate setup as a template t be imprted & reused fr ther experiment setup. Analysis: Validate the instrument type in the calibratin file & the experiment file t be the same. Ability t define cntrls in the plate t influence the aut calls Autmatically identify the pre-melt & pst melt regins upn aut call. Aut call wells that are similar t cntrls are called as the cntrl gentype Aut call wells that are different frm cntrls are called as variants. Manually assigns calls t the wells frm the Plate Layut. Ability t revert t aut call. Omit utliers frm analysis. Prvide silhuette scre fr analysis results Rev A Cpyright 2011 Life Technlgies Crpratin. All Rights Reserved. Page 2 f 5

3 v3.0.1 Release Ntes Ability t define the expected number f clusters. Create, Save & Edit multiple Analysis Settings [Pre-Melt & Pst-Melt regins] fr each target/dye cmbinatin and save in an Advanced Analysis Settings library. Sftware autmatically applies the default r saved analysis setting upn pening an experiment. Visualizatin: View Raw Melt data Plts View Derivative Melt Curves View Aligned Melt Curves View Difference Plt View Raw Melt data Plts Make a manual call frm the plts Display the analysis results in the Plate Layut under Analysis sectin and the well table fr the Experiment. Ability t mdify the pre-melt start, stp and pst-melt start, stp regins frm the & Derivative Melt Curve Plts and invke re-analysis fr each Assay. 4. SYSTEM REQUIREMENTS The cmputer hardware and perating system requirements fr the High Reslutin Melt [HRM] Sftware are: Windws XP with Service Pack 3 r Windws 7 perating systems Cre 2 Du 2.4GHz. prcessr, with 3G f RAM and 60 GB f hard drive capacity Minimum mnitr reslutin f 1280x1024 Micrsft PwerPint sftware (fr direct exprt int PwerPint slides) 5. INSTALLATION OF THE SOFTWARE HRM V3.0.1 IMPORTANT: T help prevent data lss, it is strngly recmmended that all user data are backed up befre installing the HRM v ) If yu already have HRM Sftware v3.0 installed, yu can upgrade by installing the HRM v3.0.1 using the Web Installer Rev A Cpyright 2011 Life Technlgies Crpratin. All Rights Reserved. Page 3 f 5

4 v3.0.1 Release Ntes 2) Clse any pen applicatins, and then duble-click the HRM_v3.0.1.exe file. 3) Fllw the Install Wizard nscreen installatin instructins. Yu can als install the Sftware HRM v3.0.1 using HRM v3.0.1 New Installer CD. A. If yu d nt have HRM Sftware v3.0 installed, yu can nly install the HRM v3.0.1 using HRM v3.0.1 New Installer CD. 1) Clse any pen applicatins, insert the HRM v3.0.1 New Installer CD int the CD/DVD ROM, the Install Wizard will be autmatically launched. (Nte: If the Install Wizard is nt launched, brwse the CD cntent, and duble click setup.exe t launch the Install Wizard) 2) Fllw the Install Wizard nscreen installatin instructins. 6. SUSTAINED KNOWN ISSUES The Cntrl infrmatin defined in the Plate Setup is nt persisted upn saving the Plate Set up template and applying n different experiment. If uncheck and check Aut fr the Number f Variants in the Analysis Settings, the Target cluster n reprt may shw as -1 rather than Aut in the generated reprt. If cntrls are used, the Number f Variants shuld be set t Aut in the Analysis Settings. The number f cluster settings takes precedence ver the cntrls. If a full sftware license is applied t the sftware befre the dem license expired, sftware will cntinue t display the license expiring warning message. By acknwledge the message, user will still able t cntinue t use the sftware Rev A Cpyright 2011 Life Technlgies Crpratin. All Rights Reserved. Page 4 f 5

5 v3.0.1 Release Ntes 7. LEGAL STATEMENTS Custmer s installatin r use f the HRM Sftware v3.0.1 is subject t the terms and cnditins f the sftware license agreement applicable t the HRM Sftware v Fr Research Use Only. Nt intended fr any animal r human therapeutic r diagnstic use. Ntice t Purchaser: License Disclaimer Purchase f this sftware prduct alne des nt imply any license under any prcess, instrument r ther apparatus, system, cmpsitin, reagent r kit rights under patent claims wned r therwise cntrlled by Life Technlgies Crpratin, either expressly, r by estppel Life Technlgies Crpratin. All rights reserved. The trademarks mentined herein are the prperty f Life Technlgies Crpratin r their respective wners. Micrsft, PwerPint, and Windws are registered trademarks f Micrsft Crpratin Rev A Cpyright 2011 Life Technlgies Crpratin. All Rights Reserved. Page 5 f 5

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