EVALUATION OF SHIPPING STRESS IN SURGICALLY ALTERED RODENT MODELS

Transcription

1 EVALUATION OF SHIPPING STRESS IN SURGICALLY ALTERED RODENT MODELS During Ground Transportation/ATA annual conference/orlando, FL Velu Karicheti, Ph.D. EVERY STEP OF THE WAY Venkateswarlu Karicheti, Ph.D. 1 EVERY STEP OF THE WAY

2 OUTLINE Introduction Methods Results Summary 2 EVERY STEP OF THE WAY

3 INTRODUCTION Every step of the way

4 TRANSPORTING RODENTS FOR RESEARCH Truck Transportation causes stress which in turn results in physiological changes Blood pressure Heart rate Blood glucose Corticosterone Immune function Body weight Effects of shipping stress are measured after transportation due to the technological limitations. Very limited knowledge of the stress DURING the transportation Ref: Arts J. W., Bartholomeus M. V. D., Meulen A., Kramer K., Ohl F. (2016). Transportation in Laboratory Rats: Effects of a Black Box, ed Arts J. W., editor. [dissertation thesis] (Utrecht: Brain Center Rudolf Magnus; ),Contain ~90 references on shipping stress. 4 EVERY STEP OF THE WAY

5 EFFECTS OF STRESS DURING TRANSPORTATION What do we know? Source/Supplier Transportation Destination/Scientist Objective: To evaluate stress prior to shipment, during transportation and after shipment in rodents following surgery We measured Heart Rate and Body Temperature 5 EVERY STEP OF THE WAY

6 METHODS Every step of the way

7 Real-Time Telemetry Heart Rate and Temperature Transmitter was used Manufacturer : Starr-Oddi Model: DST microrf-hrt METHODS Heart Rate and Temperature recording Key features Flexible setup, allows group housing Transmits and logs data in memory Leadless and small (3.3g) Long battery life typically 2.5 months ECG derived heart rate 7 EVERY STEP OF THE WAY

8 METHODS Animals and Surgical Description Animals Male CRL CD Sprague Dawley rats, 8-10 week, variable weight Group A (n=6) Control - data logger implants only Group B (n=6) Surgery - jugular vein catheter and data logger implantation Anesthesia Ketamine (75 mg/kg) and xylazine (6.0 mg/kg) intraperitoneal Analgesia Burprenorphine (0.02 mg/kg) subcutaneous Surgery Jugular vein catheter Jugular vein was isolated and ligatures were placed using non-absorbable suture A phlebotomy was made in the jugular vein, a polyurethane catheter was inserted, and secured The extravascular portion of the catheter was tunneled subcutaneously to the dorsal scapular region The catheter was locked with heparinized dextrose solution and the skin incision was closed using a subcuticular suture. Data logger implantation The data logger was subcutaneously tunneled to the left chest (close to apex of the heart) 8 EVERY STEP OF THE WAY

9 METHODS Post Surgical Activities Day Surgery & Recovery Ship Delivery Housing CRL, Raleigh, NC Pfizer, Groton, CT Day0 Immediately following surgery, assessments were completed daily. The behavioral observations include movement, posture, body condition, respiration etc. Day3 Physical exam was performed including body weight assessment. The rats were packed and shipped from CRL, Raleigh, NC During shipping, events such as on-road inspections, transfer to holding areas, and other significant events were logged. Day4 Rats were delivered to the Pfizer, Inc. Groton, CT site where they were unpacked, underwent a physical exam, body weight assessment, and placed in home cages. Significant events as well as behavioral observations within the housing room were recorded until end of study. 9 EVERY STEP OF THE WAY

10 METHODS Animal Packing and Truck Transport Temp Tracker Attached inside top lid Animal Crate Climate controlled vehicles Temperature readings in cab Remote monitoring long hauls GPS vehicle tracking Departure Truck 10 EVERY STEP OF THE WAY Inside of the Truck Delivery Truck

11 METHODS Transportation events Day3 1:00 pm - Start loading truck at CR Raleigh, NC 2:00 pm - Truck departed CR Raleigh, NC 11:30 pm -Arrive DSI Linden, NJ 11:30pm to 2:00am Holding Destination Groton, CT Holding Linden, NJ Day4 02:00 am - Delivery vehicle departed DSI Linden, NJ 03:00 am - Crossing George Washington Bridge 06:00 am - Arrived Pfizer, Groton, CT 06:20 am - Delivery completed Origin Raleigh, NC 18 hours travel time 11 EVERY STEP OF THE WAY

12 RESULTS Every step of the way

13 RESULTS Temperature and Heart Rate Day Surgery & Recovery Ship Deliver Housing CRL, Raleigh, NC Pfizer, Groton, CT 13 EVERY STEP OF THE WAY

14 RESULTS Temperature The body temperature changes directly relate to the drop in ambient temperature inside of the transport truck. The body temperatures from both Group A and B are same throughout transit Group A & B are similar - Correlation coefficient of EVERY STEP OF THE WAY

15 RESULTS Heart Rate Diurnal patterns changed during transit Diurnal patterns returned to normal within 3 days after arrival at the destination facility Surgical group (B) displayed higher heart rate values compared to non-surgical group (A) 15 EVERY STEP OF THE WAY

16 RESULTS Heart Rate Group B consistently maintained a higher heart rate value than Group A. T-test analysis showed that Group B and A were statistically different from one another Both groups proportionately changed during pre-shipment, transit, post-transit. 16 EVERY STEP OF THE WAY

17 SUMMARY Take Home Message Diurnal patterns disturbed during transit Both the surgical (B) and non-surgical group (A) responded similarly with temperature and heart rate values during transportation. Surgical group (B) displayed higher heart rate values compared to non-surgical group (A) The changes in heart rate (the physiologic response) during shipping was independent of the surgical status Novel method is used to collect physiological data during the transportation 17 EVERY STEP OF THE WAY

18 ACKNOWLEDGEMENTS Thank You Steve Kreuser- Pfizer Jan Bernal - Pfizer Evan Pagano - Pfizer Cassandra Rider - Pfizer Amber Kang Charles River Asgeir Bjarnason Star-Oddi CONTACT INFO Velu Karicheti, PhD Sr. Manager, Surgical Technical Services velu.karicheti@crl.com Office : Mobile : EVERY STEP OF THE WAY