Gel Permeation Chromatography GPC-System for Sample Preparation - Type VARIO Overview of Contents:
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1 Gel Permeation Chromatograhy GPC-System for Samle Prearation - Tye VARIO Overview of Contents: Automated GPC: Main Characteristics Benefits for the User Processing Sequence and Configuration Handling and System Control Technical Data / System Comonents what is next? Order Details age 1 of 14
2 Automated GPC-System, Tye VARIO Main characteristics Benefits for the user The rocessing sequences for the samle rearation for esticide analysis are described within the AOAC method No , USEPA SW-846 method 3640A, EN 12393, EN 1528 and within the L method in accordance with 35 LMBG. After extraction, these methods usually require the clean-u of the extract via a gel ermeation chromatograhy (GPC) system. LCTech have develoed a suitable GPC-system for this rocessing ste. The system, however, can be utilised in many different areas, for examle in universities or in R&D of industry. This has been realised through the remarkable flexibility of hardware and software emloyed in the system design. Flexible... The GPC-system, tye VARIO, allows for variable set-us, for examle with regard to the column size used, injection volume, flow rate and other imortant arameters. The samle carousel of the combined autosamler/fraction collector can be easily exchanged. Consequently, either more samles with smaller main fractions or fewer samles with larger main fractions can be rocessed. Samles can be transferred quantitatively. The samles are rovided in 5 ml vials (rack P60-5), which are rinsed additionally to assure a comlete transfer. Easy to oerate... Terminal Great effort has been ut into the design to make it uncomlicated and easy to understand and oerate. Via menu-driven terminal inut, all imortant arameters can be defined. To facilitate individual samle rearation, each single samle can be arameterised and saved as a method. U to 97 methods can be stored. Parameterisation is erformed via terminal inut on the system with automatic data storage. Desite the large flexibility, rocessing of samles or samle sequences may be defined within only a few minutes. The system is initiated when switched on. The function of all essential comonents is being checked. age 2 of 14
3 Robust for oeration without suervision To warrant 24-hour-oeration without suervision, all comonents have been designed to be very robust. All arts wetted by solvents are solvent resistant; materials used are glass, PTFE, stainless steel and PPS-E2. All solvent carrying ies are visible and therefore easily insected and likewise easily exchanged. As a benefit of the robust design, no daily or weekly service needs to be carried out. User rotection... The system design is articularly considerate of user rotection. Samle vials and recetacles for fractions can be (but don't have to be) sealed with seta, such that solvent evaoration is reduced to a minimum. For this reason, the GPC can be oerated indeendent of an exhaust hood. In addition, sealing with seta revents evaoration and, consequently, the unintended concentration of the samles rior to their rocessing.... and just in case the software udate or suort from LCTech is quickly and inexensively available via modem. In this way, service costs can be dramatically minimised in the long run. Injection ort with artial view of the carousel rack Summary... The GPC-system, Tye VARIO, is designed to be esecially robust for daily routine oeration in the laboratory. It is user friendly, quiet and roduces low emission levels. Consequential costs are excetionally low. age 3 of 14
4 Processing Sequence and Configuration Comrehensive automatisation according to individual requirements The automated GPC-system, Tye VARIO by LCTech comrises the following stes: Suction of the samle out of a closed vial Transfer into a samle loo Clean-u via the GPC-column and Filling into a closed container with the otion of fractionation in u to 26 vials Flexibility through modular system and intelligent software The GPC-system contains all necessary comonents, which include a high quality LCum, a combined autosamler/fraction collector and a GPC-column (unfilled). Process engineering and safety engineering of all relevant comonents have been undertaken in such a way that unattended over-night oeration is ossible. The range of alication of the GPC-system can be easily adjusted to the analytical needs by choosing the aroriate carousel rack. Deending on the caacity of the carousel rack 12 crude extracts (P ) can be rocessed in one fraction of u to 250 ml or 26 crude extracts (P ) in one fraction of u to 110 ml, or 52 crude extracts (P-60-16) in one fraction of u to 60 ml, or 8 crude extracts (P R) in one fraction of u to 250 ml (standard round-bottomed flask), or 12 crude extracts (P-100S-16) in one fraction of u to 100 ml (ointed flask) or quantitative samle transfer 52 samles (P-60-5) quantitatively from 5 ml to 60 ml vials can be rocessed. age 4 of 14
5 Carousel rack With each tye of carousel rack, the timecontrolled division of the main fraction in u to twenty-six fractions can be re-set (volume results from flow rate x time). Overfilling of the vials is automatically revented by an internal lausibility enquiry of the system. Each fraction is filled into a searate vial. Additionally, it is ossible to arameterise unrelated fractions and to vial these searately. Thereby, nearly all thinkable GPC alications should be covered. The carousel racks are easily removed and exchanged through undoing the locking device. Before every sequence, the used rack is queried by the system, which the user confirms by a keystroke. age 5 of 14
6 Handling and System Control Simle handling and fast rogramming The rack is inserted into the system and loaded. The method to be alied to the samles is simly re-selected from the system memory - either individually er samle or, in case of the same rocessing requirements, for the entire sequence. Thereby, the rocessing stes can be defined individually within a very short eriod of time. System arameterisation Parameterisation is conducted via terminal inut on the system followed by automatic storage. Further set-u otions of the GPC-system, Tye VARIO are: Pum flow rate; the system is offered inclusive of a high-quality, rearative or analytical um. Forerun and tailing from s Main run (can be divided into u to 26 fractions) from s, whereby in combination with the set flow rate, a total volume in resect of the container sizes cannot be exceeded. Variable deth of needle immersion for sedimenting samles Samle loading volume No Carry-Over The cleansing of the system is erformed after each samle, or else, as and when required. The PTFE-rinsing ort including waste function, the needle rinsing ort and all contaminated comonents are cleaned in the rinsing mode. A cross-contamination can be reliably avoided due to the comrehensive rinsing rocesses. The rinsing rocesses require a minimal amount of solvents. Should a run be interceted (e.g. by a ower-cut), then an integrated rinsing rogram can be called u. The aaratus is cleaned and reared for the next samle searation. Service Warranty exires after 12 months and a service contract with limited costs will be offered to our customers. age 6 of 14
7 Technical Data / System Comonents Measurements and weight, electrical connection 750 x 600 x 1000 mm (W x D x H), ca. 60 kg Connection 1x three-in lug 230 V AC; 50 Hz; CE-certified GPC Pum The system contains a robust SPS-controlled dulex um, which has already roved itself many times to be excellently suited for continuous running. This is either a rearative (to be set anywhere between 0.40 and ml/min) or an analytical (from ml/min) um. The um flow rate can be set, ulsation lies below < 1 %. Automatic cut-off on reaching certain thresholds for overressure or underressure is art of the system standard equiment. The um is solvent resistant and low in maintenance. Chromatograhy Column Refillable, DIN-comliant secial column (unfilled; standard delivery), materials subjected to wetting with solvents are glass and PTFE, 1/8 -PTFE-tube at inlet and outlet site, rigid PTFE-base and adjustable lunger, stable construction, high overressure stability, wearing arts are individually available. If required, any other column may be built in, if it is designed and ermitted for use under ressure of at least 200 si. The column is not filled. Filling with Bio-Beads, Tye S-X3, is recommended. Simle Construction dismantle/assemble without tools Safety and Stability (u to 40 bars) by aluminium cladding Tube Easy Adjustment of the column bed Chemically inert contact of the samle only with glass and PTFE age 7 of 14
8 Software VarioSoft (otional) The LCTech PC-software VarioSoft rovides a comfortable solution for the control of the GPC VARIO system. Process arameters can be visually assisted reselected and stored. A samle rocessing list can be created and stored offline as a job list. Facing the control at the system, now an essential extension is the definite correlation of samle identification and job list (consecutive and nonmaniulable samle ID). Protocols of the results can be exorted as ASCI-files. Samle information such as laboratory internal identification, customer data or information regarding samling and samle origin (free text inut), resectively, can be read in via barcode reading. Admission to the system is assword secured via a multi-level authentication hierarchy The Software can also be used for the integration of an UV detector for quality assurance. A fixed wavelength detector (254 nm) is recommended. Simle chromatograms will be generated, that can be used for the evaluation of the system erformance. Windows 98 or higher is required. A free disk sace of about 100 MB will be needed for the software on the hard drive. Fixed Wavelength UV Detector (otional) The UV detector offered by LCTech is otimally suitable for utilization with the LCTech software VarioSoft. It is a 254 nm fixed wavelength detector, which is tuned for the quality assurance of GPC columns. age 8 of 14
9 Combined Samler/Fraction Collector and Injection Port A robot swivel arm with a stable, sring-loaded stainless steel needle and aeration device takes u the samle, injects it into a stainless steel injection ort with needle guide and then conducts fractionation. The carousel racks can be easily mounted and taken off. The solid construction of the racks kees the vials safely in lace. Normally, vials with seta are being used that the user is not exosed to health damaging solvent vaours escaing from oen vials. Samle Loo The sulied standard samle loo is made from PTFE and is set to 5.0 ml (rearative system) or set to 1.0 ml (analytical system). A measuring rotocol is sulied. Other volumes are available on request. The samle loos can be exchanged without any effort. What is next? If the samles need to be concentrated after rocessing, the fractionating vials can be directly transferred into the analytical rearation system EVA III. This system concentrates the samles fully automatically and without the need for suervision to a defined end volume (1.0 ml, 5.0 ml or 10 ml). Solvent exchange may be incororated into this rocess. The exchange is normally conducted in the liquid hase. However, the samles may also be evaorated to dryness and then taken u by a different solvent. age 9 of 14
10 Order Details System Gel Permeation System (GPC), Tye VARIO, Basic equiment with rearative HPLC-Pum (flow rate 0.40 to 40.0 ml/min); with unfilled chromatograhy column; samle loo 5 ml; 230 VAC, 50 Hz. Carousel rack, bottles for loading the carousel rack and waste flask need to be ordered searately. Order Number GPC-VARIO Gel Permeation System (GPC), Tye VARIO, Basic equiment with analytical HPLC um (flow rate 0.10 to 9.95 ml/min); with unfilled chromatograhy column; samle loo 1 ml; 230 VAC, 50 Hz. Carousel rack, bottles for loading the carousel rack and waste flask need to be ordered searately. Order Number GPC-VARIO-A Samle Racks, combined version: autosamler and fraction collector for 12 samles, Samles in 16 ml-vials/ fractions in 250 ml-bottles (62x146 mm) Order Number: P for 26 samles, Samles in 16 ml-vials/ fractions in 110 ml bottles (42 x 146 mm) Order Number: P for 52 samles, Samles in 16 ml-vials/ fractions in 60 ml bottles (28 x 146 mm) Order Number: P for 52 samles - samle resentation in 5 ml-vials (round bottom vials) for quantitative transfer / fractions 60 ml (28 x 146 mm) consists of rack P with reduction inserts P/N (52 cs) Order number: P-60-5 for 12 samles samle resentation in 16 ml-vials / fractions in ear shaed bottom flasks, 100 ml Order Number: P-100S-16 for 8 samles, Samles in 16 ml-vials/ fractions in standard flasks, round-bottomed 250 ml Order Number: P-250R-16 Adater / Reduction insert suitable for Rack P/N P-60-16, for Vials P/N V0005 / round bottom Order Number: age 10 of 14
11 Recommended Accessories Stable, mobile table Weight ca. 30 kg; dimensions 1150 x700x800 mm (WxHxD). Order Number: Tisch-01 Modem for remote maintenance, for ISDN telehone connection incl. sulementary software Order Number: Modem-ISDN VarioSoft PC-Software to control GPC Vario and to store Samle ata and Process Parameters into a LIM S comatible, encryted ASCII- Datasheet; GPC Analysis can be suervised via UV-Detector couled via Software Interface; i.e. no additional measurement Interface for UV-Detector signal is necessary. PC-Requirements: Windows 98SE or higher; 2 GPc Hard Drive; one RS232 Interface (Com-Port); CD Rom Order-Number: UV-Fixed Wave Length-Detector, 254 nm; with semire cell, 44 µl volume (Cable No is necessary) Order-Number: Cable, RS 232 to connect UV-Detector Order-Number: Column D25 Column D25, unfilled, column ID 25 mm, OD 40 mm, filling length between 305 and 365 mm Order Number GPC10010 Column D25, filled with 50 g Bio-Beads Order Number GPC10011 Column filling kit Order Number GPC10012 Refill (Column GPC10010) with 50 g Bio-Beads Order Number GPC10013 age 11 of 14
12 Column D10 Column D10, unfilled, column ID 10 mm, OD25 mm, filling length between 300 and 365 mm Order Number GPC20010 Column D10, filled with 10 g Bio-Beads Order Number GPC20011 Column filling kit Order Number GPC20012 Refill (Column GPC20010) with 10 g Bio-Beads Order Number GPC20013 Bio-Beads, SX-3, mesh size, VE = 100 Order Number GPC10001 Samle loo 5.0 ml, Samle loo with fitting, adjusted to 5 ml, measuring rotocol included Order Number INJ-5 Samle loo 1.0 ml, Samle loo with fitting, adjusted to 1 ml, measuring rotocol included Order Number INJ-1 Bottles for solvent suly and solvent recovery Laboratory bottle made of glass, ml, screw thread GL 45 for solvent suly; with secial sealing ca with searate otions for the GPC um and the syringe um; 1/8 "-connection Order Number F10000-DV Laboratory bottle made of glass, ml, screw thread GL 45 with secial sealing ca; connectable to waste-line Order Number F10000-D age 12 of 14
13 Recetables for fractions; suitable for the aroriate rack Screw-thread bottle, Nominal content about 250 ml, with sealing ca (GL25) with aerture for seal suitable for samle rack P ; 2 bottles er carton (= 1 selling unit) Order Number F-250- Screw-thread bottle, with residue removal (concave bottom) Nominal content max. 120 ml; with sealing ca (GL25) with aerture for seal suitable for samle rack P ; 2 bottles er carton (= 1 selling unit) Order Number F-100-D Screw-thread bottle, Nominal content about 60 ml, with sealing ca (GL24) with aerture for seal suitable for samle rack P ; 144 cs. er carton (= 1 selling unit) Order Number F-060-D Sealing ca with hole (for seal break-through) suitable for all screw-thread bottles F-110-D and F-200-D (GL25) Packing unit: 10 ieces (= 1 selling unit) Order Number V-0025-SL Sealing ca with hole (for seal break-through) suitable for all screw-thread bottles F-060-D (GL24) Packing unit: 144 ieces (= 1 selling unit) Order Number V-0024-SL Secial-Seals suitable for all screw-thread bottles F-200, F110-D und F-060-D Packing unit: 100 ieces (= 1 selling unit) Order Number V-0025-D Samle vials Screw-thread vials, 16 ml, flat bottom, oen without seal suitable for all racks P Packing unit 100 cs (= 1 selling unit) Order Number V-0016 Screw ca with hole, black for 16 ml-vials (Part-No.: V-0016), Packing unit 100 cs (=1 selling unit) Order Number V-0016-SL age 13 of 14
14 Seal G18 for 16 ml-vials PTFE/silicone, red/white, for 16 ml-vials (Part-No.: V-0016), Packing unit 100 cs (=1 selling unit) Order Number V-0016-D Screw ca without hole, black for 16 ml-vials (Part-No.: V-0016), Packing unit 100 cs (=1 selling unit) Order Number V-0016-S Injection vials, clear glass, 1,5 ml for GC-systems Crim-to vials 11 x 4 mm Dimensions 32 x 11, 5 x 0,9 mm Packing unit 100 cs (=1 selling unit) Order Number V-0001 Crim seals, R11, with inert seal washer PTFE/Silicone/PTFE, red Packing unit 100 cs (=1 selling unit) Order Number V-0001-B age 14 of 14
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