Welchrom Solid Phase Extration

Transcription

1 Welchrom Solid Phase Extration Solid Phase Extraction Cartridge Sample preparation is one of the most important key steps for the entire analysis process as over 60% of the entire analysis process time and over 30% of the analysis errors are from the sample preparation. Over the last twenty years, SPE has become the most powerful technique prior to analytical chromatography for the cleanup, purification, and concentration of samples from various matrices, including urine, blood, water, beverages, soil, and animal tissue. Solid phase extraction is a form of digital (stepwise) chromatography designed to extract, partition, and/ or adsorb one or more components from a liquid phase (sample) onto stationary phase (sorbent or resin). SPE extends a chromatographic system s lifetime, improves qualitative and quantitative analysis, and by changing an analyte of interest s original matrix environment to a simpler matrix more suitable for subsequent analysis, the demand placed on an analytical instrument is considerably lessened. Use SPE for Samples that: Contain particulate matter causing system clogging and high back-pressure. Contain components that cause high background, misleading peaks, and/or poor sensitivity. Require cleanup, trace enrichment/concentration, or purification. Require sample matrix or solvent. Benefits of SPE: Switch sample matrices to form more compatible with chromatographic analyses. Concentrate analytes for increased sensitivity. Remove interferences to simplify chromatography and improve quantitation. Protect the analytical column from contaminants. Common SPE Applications: Pharmaceutical compounds and metabolites in biological fluids. Drugs of abuse in biological fluids.l Environmental pollutants in drinking and wastewater. Pesticides and antibiotics in food/agricultural matrices. Desalting of proteins and peptides. Fractionation of lipids. Water and fat soluble vitamins. Comparison Between SPE and HPLC Items HPLC SPE Tube Stainless steel tube Plastic tube Particle Size( μm) 3,5, Particle Shape Spherical Amorphous Column Efficiency <100 Separate Theory Continuous Elution Digital Switch Elution Price($) Usage Mode Reusable Disposable Operate Cost High Low Equipment Cost High Low 10

2 Introduction of Products Since 2005, Welch Materials, Inc. has launched Ultisil (also called Ultimate in China market), Xtimate, and TopsilTM series of HPLC chromatography column packing and products into the China market, with excellent chromatographic performance, competitive price and the best professional services. Those products have won the trust from the majority of users. With several years of increased market share, Welch Materials now has become one of the top several major suppliers of HPLC column product line in China. Based on our core chromatographic packing technology and strong new product development ability, Welch Materials also developed a series of products (Welchrom is a registered trade mark of Welch Materials), including silica-based, polymer-based products, and other inorganicsorbent, and specialty and mixed phases. products are manufactured using state-ofthe-art automation to guarantee quality and consistency. We have high standards of a series of quality assurance measurements to characterize raw materials and semifinished packing, such as pore size, pore volume, surface area and the carbon loading, to ensure products excellent batch-to-batch reproducibility and high extraction efficiency. Selection Guide 11

3 Welchrom Solid Phase Extration(SPE) Base Material Phase Category Silica-Based Polymer- Based Specialty and Mixed Mode Bonded Functional Group Endcapped Carbon Loading (%) Surface Area (m 2 /g) Particle Size (μm) C18E Non-polar C18 Yes C18 Non-polar C18 No C8 Non-polar/polar C8 Yes Phenyl Polar/Non-polar Phenyl Yes CN Polar CN No Diol Polar Diol No NH 2 Polar Aminopropyl No PSA Polar/Weak anion Ethylenediamine -n-propyl Pore Size (A) No Silica Polar Silica N/A N/A SAX Polar/Anion Trimethylaminopropyl No SCX Cation Benzenesulfonic Acid No Weak cation Carboxylic Acid Yes PRS Cation Propylsulfonic Acid No Non-silica Inorganicbased Florisil Polar Florisil N/A N/A N/A Florisil PR Polar Florisil/Na 2 SO 4 N/A N/A N/A Alumina-A Alumina acidic N/A N/A N/A N/A Alumina-B Alumina basic N/A N/A N/A N/A Alumina-N Alumina neutral N/A N/A N/A N/A Celite Polar Celite N/A N/A N/A BRP P-SAX P-SCX P-WAX P- PS/DVB GraphiCarb GraphiCarb/ NH 2 C8/SCX non-polar/polar Anion / Polar Cation / Polar/ Weak cation / Polar Weak antion / Polar Polar/non-polar Strongly nonpolar/anion Non-polar/Polar/ Anion Non-polar/Cation Hydrophilic Styrene Divinylbenzene N/A N/A N/A Sax Functionalized N/A N/A Scx Functionalized N/A N/A Wax Functionalized N/A N/A Wcx Functionlized N/A N/A Styrene- Divingbenzene N/A N/A Graphitized Carbon N/A N/A 100 Graphitized Carbon/ Aminopropyl N/A No N/A C8/SCX No N/A C8/SAX Non-polar/Anion C18/SiO 2 No N/A

4 Cross Reference of Comparable Phases By Manufacture Phenomenex Strata Waters Sep-Pak Silica-based and other Sorbents Supelco Supelclean/Discovery Agilent Bond Elut Agela Cleanert Welch Welchrom C18-E tc18 ENVI-18,DSC-C18,LC-18 C18 C18 C18E C18-U C18 C18OH C18-N C18 C8 C8 DSC-8,ENVI-8,LC-8 C8 C8 C8 Phenyl DSC-Ph, Lc-Ph PH Phenyl Screen-C Certify C8/SCX Si-1 Silica DSC-Si, LC-SI SI Silica Silica FL-PR Florisil LC and ENVI Florisil FL Florisil Florisil NH 2 Amino Propyl DSC-NH 2,LC-NH NH 2 NH 2 NH 2 DSC-Diol, LC-Diol 2OH Diol Diol CN Cyano Propyl DSC-CN, LC-CN CN-U CN CN Alumina A, B, N LC-Alumina A, B,N Alumina A, B, N Alumina-A, B, N SAX AccellPlus QMA DSC-SAX, LC-SAX SAX SAX SAX SCX AccellPlus CM DSC-SCX, LC-SCX SCX SCX ENVI-Carb Carbon PestiCarb GraphiCarb ENVICarb-II/NH 2 Carbon/NH 2 Graphicarb/NH 2 Polymers Phenomenex Strata Waters Oasis Supelco Supelclean/Discovery Agilent Bond Elut Agela Cleanert Welch Welchrom Strata-X HLB Plexa BRP SDB-L ENVI-ChomP ENV/PPL PS PS/DVB Strata-X-C MCX Plexa PCX PCX P-SCX MAX Plexa PAX P-SAX Strata-X-CW P- Strata-X-AW WAX P-WAX 13

5 Welchrom Polymeric SPE Welchrom polymeric SPE is made from monodisperse polymer beads, which surface is modified by the Welch's uniquesurface modification and functionalization technology to produce various types of polymer SPE sorbents. Welchrom SPE polymer beads have accurate Particle Size of 40µm with a high degree of uniformity of Particle Size and pore size, excellent surface area,and the optimal bonding density of functional groups, which can meet high sensitivity analysis requirement for a wide variety ofapplications of acidic, neutral and basic compounds. Comparison Between Welchrom Polymeric SPE And Traditional SPE Welchrom polymeric SPE Traditional SPE High detection sensitivity Long column lifetime Quality guaranteed Good reproducibility Time saving Specific selectivity Decrease solvent consumption Decreased blow-down time Decreased sample Variation ph Stable from 1-14 No afraid if accidently dried in the experimental process No contamination, no hydrolysis of the bonded phase like silica substrate Welchrom Polymeric SPE Sorbents Phase BRP PS/DVB P-SCX P-SAX P- P-WAX Phase Description Reversed phase sorbent that allows for aggressive sample cleanup and high recoveries of polar and nonpolar compounds with high loadability. Highly crosslinked Polystyrene/divinyl benzene copolymer for rapid adsorption and separation of hydrophobic substances. Strong cation- sorbent that very strongly retains positively charged compounds, allowing for 100 % organic washes. Strong antion- sorbent that retains a wide range of acid compounds. Weak cation- sorbent that retains a wide range of basic compounds, including quaternary amines. Weak anion- sorbent that retains a wide range of acidic compounds. 14