grids for electron microscopy

Transcription

1 em grids

2 0 grids for electron microscopy TEM Grids - Square mesh grids, pages Parallel line grids, pages Parallel line grids with central dividing bar, pages Slotted mesh grids, pages Folding grids, pages Hexagonal grids, pages Solid/slot grids, pages Finder grids, pages 4 - Freeze fracture grids, page -6 - Reference pattern grids, pages Co-ordinate grids, page Various specialist patterns Custom Patterns: If you need something different from the patterns in this catalogue there is no problem, we have a very cost-effective custom reticle facility that is able to make the exact pattern you require. Pyser Optics Limited (were Graticules Ltd) have been manufacturing specimen support grids for electron microscopes for over 4 years using the extensive knowledge of processes and techniques gained from their micropattern production. In 1994 Pyser took over the production of grids made in Holland by Brandsma BV (Known as Embra grids) and as a result offer a wide range of grids in a variety of metals and finishes to meet the majority of requirements. More recently Pyser has introduced carbon coated grids as well as a Value range for the larger user. Pyser grids are available throughout the world from laboratory and electron microscopy accessory suppliers or direct from our factory., nickel and gold grids are all produced using electroforming processes to give well-defined patterns and consistent quality. Aluminium, stainless steel, molybdenum and titanium use an etching process. Most grids are supplied in anti-static vials of 100 pieces, except for:- Etched grids in aluminium, molybdenum, stainless steel, Embra gold and titanium, which are in vials of pieces. The etched grids can easily be identified in the following section by GG, GM, GT, GA & GS appearing in the bracketed erence, i.e. [1GT7]. Value range of grids, which are supplied in vials of 10 pieces, in copper and nickel. Carbon coated grids, which are supplied in indexed grid boxes of 0 pieces and supplied with index card.

3 03 Apart from grids coated on one side with rhodium to prevent tarnishing, all grids in copper, nickel and gold are matt on one side, bright on the other side. All Maxta/Maxtaform grids have a smooth surface (shiny finish) and an outer ring width (frame) of 0-300μ. Maxta or Maxtaform grids are defined by an H, HF or HR prefix and all have an 03D.... All Embra grids have a rough surface (matt finish) and an outer ring width (frame) of µ. Every grid manufactured by Pyser is individually inspected under a minimum of x magnification and packed in anti-static vials. The only exception are the Value range, which are sample inspected. All patterns are supplied as mm diameter grids. We are also able to supply.3mm grids (Siemens G range) to special order. All grids are approximately micron thickness, with the exception of stainless steel which is 1µ and titanium which is 1µ. In the following section you will note that cross-references are given to relate the i.e. 07D00901 to the erences i.e. [1GC0]. Please note that specifications given for etched grids in titanium, molybdenum, aluminium and stainless steel are given for guidance only as small variations can occur. central indexing marks All grids with a reference starting1g and our Value range have a central reference mark, HF and HR patterns do not. reference and finder grids. Pyser were the first company to introduce referenced graticules for light microscopy and the first to make finder grids for the electron microscopist. Location of points of interest in a specimen and quick relocation is of vital importance in many investigations and with the high magnifications used in transmission electron microscopy this can be very tedious. Finder grids have been designed to assist in this task and there are today many types to choose from. We list those that have proved to be most popular. handled grids By bending the handle upward the grid can be gripped by fine tweezers without the latter touching the specimen or any liquids. The grid can be placed over or underneath the specimen without surface tension causing the grid to slide up wetted tweezers. Grids can be held by their tab for spraying, rinsing and drying. The handle is also angled to assist in the alignment of the grid bars parallel to the axes of the stage. carbon coated grids Pyser Optics has a standard range of plain mesh grids with carbon coating. Additionally many of our other grids can be supplied with this coating to special order. sem grids SEM s often require finder grids to place on pin stubs to assist in locating specimens of interest. These are generally 1mm diameter and we can offer standard products or custom made patterns to suit your exact requirements. custom designs Pyser are able to manufacture grids to your exact requirements. Just send in your specification detailing the pattern, bar thickness, material and quantity and we will provide a quotation

4 04 plain mesh grids 0 mesh Molybdenum Aluminium [1GC 0] [1GN 0] [1GG 0] [1GT 0] [1MC 0] [1GA 0] [1MS 0] 07D D D L L L L G_ mesh with Rhodium (high grade) (high grade) Molybdnum Aluminium [1GC 7] [HF 31Cu] [HR 1Cu/Rh] [1GN 7] [HF 31Ni] [HR 1Ni] [1GG 7] [HR 1Au] [1GT 7] [1GM 7] [1GA 7] [1GS 7] 07D D D D D D L D L L090 08L L0690 HF HR G_ mesh with Rhodium (high grade) (high grade) Molybdnum Aluminium [1GC 100] [HF 3Cu] [HR Cu/Rh] [1GN 100] [HF 3Ni] [HR Ni] [1GG 100] [HR Au] [1GT 100] [1GM 100] [1GA 100] [1GS 100] 07D D006 03D D D D D D L L L L06903 HF HR G_

5 0 plain mesh grids 10 mesh with Rhodium (high grade) (high grade) Molybdnum Aluminium [1GC 10] [HF 33Cu] [HR Cu/Rh] [1GN 10] [HF 33Ni] [HR 3Ni] [1GG 10] [HR 3Au] [1GT 10] [1GM 10] [1GA 10] [1GS 10] 07D D D D D006 03D D D L L L L06904 HF HR G_ mesh with Rhodium (high grade) (high grade) Molybdnum Aluminium [1GC 00] [HF 34Cu] [HR 4Cu/Rh] [1GN 00] [HF 34Ni] [HR 4Ni] [1GG 00] [HR 4Au] [1GT 00] [1GM 00] [1GA 00] [1GS 00] 07D D D D D D D090 03D L L090 08L L0690 HF HR G_

6 06 plain mesh grids 300 mesh with Rhodium (high grade) (high grade) Molybdnum Aluminium [1GC 300] [HF 3Cu] [HR Cu/Rh] [1GN 300] [HF 3Ni] [HR Ni] [1GG 300] [HR Au] [1GT 300] [1GM 300] [1GA 300] [1GS 300] 07D D D D D D D090 03D L L090 08L L0690 HF HR G_ * mesh with Rhodium (high grade) (high grade) Molybdnum Aluminium [1GC 400] [HF 36Cu] [HR 6Cu/Rh] [1GN 400] [HF 36Ni] [HR 6Ni] [1GG 400] [HR 6Au] [1GT 400] [1GM 400] [1GA 400] [1GS 400] 07D D D D D D D090 03D L L090 08L L0690 HF HR G_ * 3 00 mesh [1GC 00] [1GN 00] [1GG 00] 07D D D0908 1G_

7 07 plain mesh grids 600 mesh [1GC 600] [1GN 600] [1GG 600] 07D D D G_ mesh grids with handles 0 mesh Molybdnum Aluminium [3HGC 0] [3HGN 0] [3HGG 0] [3HGT 0] [3HGM 0] [3HGS 0] [3HGA 0] 07D D D L L L L HG_ mesh Molybdnum Aluminium [3HGC 7] [3HGN 7] [3HGG 7] [3HGT 7] [3HGM 7] [3HGS 7] [3HGA 7] 07D D D390 08L L390 08L L3490 3HG_ mesh [3HGC 100] [3HGN 100] 07D D HG_

8 08 mesh grids with handles 10 mesh [3HGC 10] [3HGN 10] [3HGG 10] 07D D D3904 3HG_ mesh Molybdnum Aluminium [3HGC 00] [3HGN 00] [3HGG 00] [3HGT 00] [3HGM 00] [3HGS 00] [3HGA 00] 07D D D390 08L L390 08L L3490 3HG_ mesh Molybdnum Aluminium [3HGC 300] [3HGN 300] [3HGG 300] [3HGT 300] [3HGM 300] [3HGS 300] [3HGA 300] 07D D D L L L L HG_ mesh [3HGC 400] [3HGN 400] [3HGG 400] 07D D D3907 3HG_

9 09 mesh grids with handles 00 mesh [3HGC 00] [3HGN 00] 07D D HG_ mesh [3HGC 600] [3HGN 600] [3HGG 600] 07D D D HG_ parallel lines 4 micron pitch [1GC 100L] [1GN 100L] 07D D G_100L micron pitch [HF 4] [HF 4] 03D D00809 HF

10 10 parallel lines 17 micron pitch [1GC 00L] [HF 46] [1GN 00L] [HF 46] 07D D D D00811 pattern pitch microns mesh space microns bar microns transmission % tolerance +/- microns HF G_00L micron pitch [1GC 300L] [1GN 300L] 07D D G_300L parallel lines with handles 4 micron pitch [3HGC 100L] [3HGN 100L] 07D D HG_100L micron pitch [3HGC 00L] [3HGN 00L] 07D D31910 HF

11 11 parallel lines with handles 84 micron pitch [3HGC 300L] [3HGN 300L] 07D D HG_300L parallel lines with dividers 4 micron pitch [1GC 100LD] [1GN 100LD] [1GG 100LD] 07D D D091 1G_100LD micron pitch [HF 43] [HF 43] 03D D0080 HF micron pitch [1GC 00LD] [HF 44] [1GN 00LD] [HF 44] [1GG 00LD] 07D D D D D0913 HF G_00LD

12 1 parallel lines with dividers 84 micron pitch [1GC 300LD] 07D00914 [1GN 300LD] 07D01914 [1GG 300LD] 07D0914 1G_300LD parallel lines with dividers and handles 4 micron pitch [3HGC 100LD] [3HGN 100LD] 07D D3191 3HG_100LD micron pitch [3HGC 00LD] [3HGN 00LD] 07D D HG_00LD micron pitch [3HGC 300LD] [3HGC 300LD] 07D D HG_300LD

13 13 slotted mesh grids 0/ 00 [1GC 0/00] [1GN 0/00] 07D D0191 1G_0/00 08 x 17 0 x 00 4 x / 300 [1GC 7/300] [HF 41] with Rhodium [HR 7] [1GN 7/300] [HF 41] (high grade) [HR 7] [1GG 7/300] (high grade) [HR 7] 07D D D D D D D D0070 HR x 84 7 x x HF x 84 7 x x G_7/ x 8 7 x x / 400 [1GC 100/400] [HF 4] [1GN 100/400] [HF 4] [HF 4] 07D D D D D008 HF 4 4 x x x G_100/400 4 x x x

14 14 slotted mesh grids with handles 0/ 00 [3HGC 0/00] [3GN 0/00] 07D D3191 3HG_0/00 08 x 00 0 x 00 4 x / 300 [3HGC 7/300] [3HGN 7/300] 07D D HG_7/ x 8 7 x x 30 6 staggered grids 7/ 300 staggered with Rhodium 07D D D D0970 7/300 staggered 339 x 8 7 x x 30

15 1 folding grids 0/ 0 [4GCF 0/0] [4GNF 0/0] [4GGF 0/0] 07D D D097 4G_F0/0 08 & 08 0 x & / 100 Molybdenum [4GCF 100/100] [4GNF 100/100] [4GGF 100/100] [4GTF 100/100] [4GMF 100/100] [4GSF 100/100] 07D D D098 08L L098 08L0698 4G_F100/100 4 & x & 6 100/ 00 Molybdenum [4GCF 100/00] [4GNF 100/00] [4GGF 100/00] [4GTF 100/00] [4GMF 100/00] [4GSF 100/00] 07D D D099 08L L099 08L0699 4G_F100/00 4 & x 00 0 & & 37 6 & / 300 [4GCF 100/300] [4GNF 100/300] [4GGF 100/300] [4GTF 100/300] [4GMF 100/300] 07D D D L L G_F100/300 4 & x & 7 49 & 8 6 & 4

16 16 folding grids 100/ 400 [4GCF 100/400] [4GNF 100/400] 07D D G_F100/400 4 & x & & 6 6 & 3 00/ 00 Reference Combination of H1 and H grids in a folded format. LH grid is a standard 00 mesh, RH grid is 00 mesh reference pattern. with Rhodium [H1] [H1] [H1] 03D D D00631 H1 17/ x & & 7 hexagonal grids 90 mesh [8GC 90] [8GN 90] [8GG 90] 07D D D0948 8G_90 7 x x mesh [8GC 13] [8GN 13] [8GG 13] 07D D D0949 8G_ x x

17 17 hexagonal grids 180 mesh [8GC 180] [8GN 180] [8GG 180] 07D D D090 8G_ & x mesh [8GC 70] [8GN 70] [8GG 70] 07D D D091 8G_70 86 & x mesh [8GC 360] [8GN 360] [8GG 360] 07D D D099 4G_F100/00 4 & x 00 0 & & 37 6 & 60 H pattern [H] [H] [H] 03D D D0060 H 30 & & 37 80

18 18 hexagonal grids with handles 90 mesh [8HGC 90] [8HGN 90] 07D D HG_90 7 & x mesh [8HGC 13] 07D HG_ & x mesh [8HGC 180] [8HGN 180] [8HGG 180] 07D D D390 8HG_ & & mesh [8HGC 70] [8HGN 70] [8HGG 70] 07D D D391 8HG_70 86 & & 7 4

19 19 hexagonal grids with handles 360 mesh [8HGC 360] [8HGN 360] 07D309 07D319 [8HGG 360] 03D39 8HG_ & x solid grids 0.4 mm hole grids Molybdnum [1GC 4H] [1GN 4H] [1GG 4H] [1GT 4H] [1GM 4H] [1GS 4H] 07D D D L L L G_4H mm hole grids Molybdnum [1GC 6H] [HF 48] [1GN 6H] [HF 48] [1GG 6H] [1GM 6H] 07D D D D D L0919 HF G_6H mm hole grids [1GC 8H] [1GN 8H] 07D D0190 1G_8H 800 0

20 0 solid grids 1.0 mm hole grids Molybdnum Aluminium [1GC 10H] [HF 47] [1GN 10H] [HF 47] [1GG 10H] [1GT 10H] [1GM 10H] [1GA 10H] [1GS 10H] 07D D D D D091 08L L091 08L L0691 HF G_10H mm hole grids Molybdnum [1GC 1H] [1GN 1H] [1GG 1H] [1GT 1H] [1GM 1H] [1GS 1H] 07D009 07D019 08L079 08L09 08L049 08L069 1G_1H mm hole grids [1GC 0H] [1GN 0H] 07D D0193 1G_0H mm x 980μ slot grids with Rhodium Molybdnum Aluminium [1GC 1S] [1GN 1S] [1GCR 1S] [1GG 1S] [1GT 1S] [1GM 10H] [1GA 10H] [1GS 10H] 07D D DSPEC 07D094 08L L094 08L L0694 1G_1H 980 x 000 0

21 1 solid grids 0.4 mm slot grids Molybdnum Aluminium [1GC 4S] [1GN 4S] [1GG 4S] [1GT 4S] [1GM 4S] [1GA 4S] [1GS 4S] 07D009 07D019 07D09 08L079 08L09 08L049 08L069 1G_4H 40 x mm x 1000μ slot grids the size of type of grid with Rhodium [HF 49] [HF 49] [HF 49] 03D D D0084 HF x x 1. mm slots the size of type of grid [HF 0] [HF 0] 03D D00819 HF 0 00 x x 0. mm slots [HF 1] [HF 1] 03D D0081 HF 0 00 x 00 10

22 solid grids 7 hexagon grid the size of type of grid [1HGC 7HEX] [1HGN 7HEX] [1HGG 7HEX] 07D D D096 1HG_7HEX 610 x x solid grids with handles 0.4mm hole grids [3HGC 4H] [3HGN 4H] 07D D HG_4H 400 dia 0 0.6mm hole grids [3HGC 6H] [3HGC 6H] 07D D HG_6H 600 dia 0 0.8mm hole grids [3HGC 8H] [3HGC 8H] 07D D3190 3HG_8H 800 dia 0

23 3 solid grids with handles 1.0mm hole grids [3HGC 10H] [3HGN 10H] 07D D3191 [3HGG 10H] 07D391 3HG_10H 1000 dia 0 1.mm hole grids [3HGC 1H] [3HGN 1H] 07D D HG_1H 100 dia 0 mm x 980μ slot grids [3HGC 1S] [3HGN 1S] 07D D3194 [3HGG 1S] 07D394 3HG_1H 980 x mm slot grids [3HGC 4S] [3HGN 4S] 07D309 07D319 3HG_4S 40 x hexagon grids [3HGC 7HEx] [3HGN 7HEx] 07D D3196 pattern 3HG_7HEX please ask for information

24 4 special types 100 mesh finders [7FGC 100] 07D0093 [7FGN 100] 07D0193 [7FGG 100] 07D mesh finders [7FGC 00] 07D00933 [7FGN 00] 07D01933 [7FGG 00] 07D mesh finders [7FGC 300] 07D00934 [7FGN 300] 07D01934 [7FGG 300] 07D mesh finders [7FGC 400] [7FGN 400] [7FGG 400] 07D D D mesh high open area [11GC 00] 07D00940

25 special types 400 mesh high open area [1GC 400] 07D mesh freeze fracture [GC 7FF] 07D0094 [GN 7FF] 07D0194 [GG 7FF] 07D094 G_7FF mesh freeze fracture / Rhodium [HF 14] 03D00610 [HF 14] 03D00611 [HF 14] 03D0061 HF chien grid [9GC 0H] 07D00936

26 6 special types 10 mesh tissue processing screen diameter 3.8 mm [6GC 10] [6GN 10] 07D D G_ mesh tissue processing screen diameter 3.8 mm [6GC 00] [6GN 00] 07D D0194 6G_ mesh tissue processing screen diameter 3.8 mm [6GC 300] [6GN 300] 07D D G_ H1 pattern, 00 mesh [H1] 03D00600 [H1] 03D00601 [H1] 03D0060 H

27 7 special types H4 pattern, 00 mesh [H4] 03D0060 [H4] 03D0061 [H4] 03D006 H H9 pattern, 0 mesh [H9] 03D00606 [H9] 03D00607 [H9] 03D00608 H HF pattern, 100 mesh [HF ] [HF ] 03D008 03D0083 HF 4 x x x 46 H reference pattern, 00 mesh [H1] 03D00600 [H1] 03D00601 [H1] 03D0060 H

28 8 special types H7 reference pattern, 400 mesh [H7] 03D0079 [H7] 03D00760 [H7] 03D00761 H H6 reference pattern [H6] 03D0076 [H6] 03D0077 [H6] 03D0078 H x H1 reference pattern / Rhodium [HF 1] [HF 1] [HF 1] 03D D D007 HF special types with handles 100 mesh finders [7HFGC 100] [7HFGN 100] 07D D3093

29 9 special types with handles 00 mesh finders [7HFGC 00] 07D30933 [7HFGN 00] 07D31933 [7HFGG 00] 07D mesh finders [7HFGC 300] 07D30934 [7HFGN 300] 07D31934 [7HFGG 300] 07D mesh finders [7HFGC 400] 07D3093 [7HFGN 400] 07D3193 [7HFGG 400] 07D mesh co ordinate [7HXC 100] [7HXN 100] 07D D mesh co ordinate [7HXC 00] [7HXN 00] 07D D01938

30 30 special types with handles 300 mesh co ordinate [7HXC 300] [7HXN 300] 07D D mesh freeze fracture [HGC 7FF] [HGN 7FF] 07D D3194 value range of electron microscopy support grids - Low cost - or - 0 to 400 mesh - 10 grids per vial The VALUE range of electron microscopy support grids has been introduced by Pyser Optics to satisfy the need for quality EM grids at low prices. For over 4 years the Graticules Division of Pyser Optics has been supplying the quality needs of the EM market. The reputation of our Maxtaform and Embra grid products has always been of excellent quality for the discerning user. These ranges are our research grade products and are 100% inspected. The VALUE range has been introduced to satisfy the needs of the general purpose user who buy grids in quantity, where the very highest quality of the research grade grids is not required, and price is very important. All grids have a centre mark. mesh size material pitch (μ) bar width (μ) hole size (μ) transmission (%) D D D D D D D D D D D D Please note minimum order quantity vials of each type. All copper and nickel Value grids are supplied in vials of a minimum 10 pieces. Dimensions given are for comparative purposes only, there may be slight variations and occasional imperfections. Prices exclude shipping.

31 31 carbon coated grids Pyser Optics now offer Carbon support film coatings on their range of TEM grids. All coated grids are supplied in vials of 0 pieces and we have listed here the most common mesh grids from 00 to 400 mesh. We can of course provide this support film coating on any of our extensive range of grid products please enquire for prices. description mesh pitch microns hole microns bar microns transmission (%) (+/-%) (+/-%) (+/-%) (+/-%) (+/-%) (+/-%) Minimum order is vials (can be mixed types). Delivery typically 3-4 weeks

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