Scientific Adsorbents' Aluminas Analytical, Prep LC, Sample Processing

Transcription

1 SAI's Alumina for chromatography has contributed to the continued growing use of this material, in a diverse number of applications. Indeed, the major reasons for this growth has been our contribution to the standardization of the manufacturing process resulting in standardized grades of Alumina with very well controlled and defined: Activity (according to Brockmann): Super Activity I Standard (Std) Activity I-IV ph: Acid(A) Basic(B) (N) Stepless Deactivation behavior Constant Deactivation behavior Controlled Chromatographic Parameters Controlled Surface Area, Porosity Scientific Adsorbents' Aluminas Analytical, Prep LC, Sample Processing Super I Super Activity I Aluminas are as unique as SAI's products. They demonstrate approximately twice the capacity as compared to Standard Activity I. Surface modifications available are "A" (Acid), "B" (Basic), and "N" (). Super Activity I Aluminas constitute the starting material for the Scientific Adsorbent's line of Aluminas. Therefore, it is easy to change between various modes of chromatography. A special feature of Super Activity I is absolutely constant deactivation behavior which is valid for the deactivation process as well as when in contact with the chromatographic solvent ALUMINA A, SUPER I, u, 500g ALUMINA A, SUPER I, u, 1kg ALUMINA A, SUPER I, u, 5kg ALUMINA A, SUPER I, u, 50kg ALUMINA B, SUPER I, u, 500g ALUMINA B, SUPER I, u, 1kg ALUMINA B, SUPER I, u, 5kg ALUMINA B, SUPER I, u, 50kg ALUMINA N, SUPER I, u, 500g ALUMINA N, SUPER I, u, 1kg ALUMINA N, SUPER I, u, 5kg ALUMINA N, SUPER I, u, 50kg

2 Standard Activity I Alumina Standard Activity I (Act I) is available with various surface modifications, A, B, and N to facilitate the separation of a wide range of compounds. In addition to ph, the activity of the surface of Alumina can mediate the separation. It is simple to adjust the activity by adjusting the water content of the material. (Alternatively, other polar media can replace the water.) Use high activity Alumina (Std Act I, Super Act I) for the separation of polar samples in nonpolar solvent systems... and for the purification of solvents Use lower activity Alumina for less polar samples ALUMINA A, ACT I, u, 500g ALUMINA A, ACT I, u, 1kg ALUMINA A, ACT I, u, 5kg ALUMINA A, ACT I, u, 50kg ALUMINA B, ACT I, u, 500g ALUMINA B, ACT I, u, 1kg ALUMINA B, ACT I, u, 5kg ALUMINA B, ACT I, u, 50kg ALUMINA N, ACT I, u, 500g ALUMINA N, ACT I, u, 1kg ALUMINA N, ACT I, u, 5kg ALUMINA N, ACT I, u, 50 kg DCC Alumina DCC - Dry Column Chromatography is a versatile Prep LC method that bridges the gap between analytical TLC and preparative column chromatography. See Silica DCC for detailed procedure ALUMINA DCC, u, 500g ALUMINA DCC, u, 5kg ALUMINA DCC, u, 50kg "Flash" Alumina Flash Chromatography is a rapid Prep LC technique that facilitates the separation of g of material via simple economical laboratory protocols ALUMINA N, 40 MICRONS(FLASH), 32-63u, 500g ALUMINA N, 40 MICRONS(FLASH), 32-63u, 1kg ALUMINA N, 40 MICRONS(FLASH), 32-63u, 5kg ALUMINA N, ACTIVE, 40 MICRONS(FLASH), 32-63u, 500g Activity II, III Alumina II - III sorbents are economical adsorbents of medium activity. Use this

3 material for general purpose scouting and in cases where the use of carbon black is precluded due to its organic nature. Also, use Alumina II - III as a replacement for organic/polymeric ion exchangers, especially when it is necessary to overcome temperature and radiation cleavage problems ALUMINA ACT II-III, u, 500g ALUMINA ACT II-III, u, 5kg ALUMINA ACT II-III, u, 50kg Alumina for HPLC The following Catalog #s are recommended: ALUMINA N, 5u, 10g ALUMINA N, 5u, 100g ALUMINA N, 10u, 10g ALUMINA N, 10u, 100g ALUMINA N, 15u, 10g ALUMINA N, 15u, 100g ALUMINA N, 25u, 10g ALUMINA N, 25u, 100g ALUMINA A, ACTIVE, 18-32u, 100g ALUMINA B, ACTIVE, 18-32u, 100g ALUMINA N, ACTIVE, 18-32u, 10g ALUMINA N, ACTIVE, 18-32u, 100g Alumina Oxides for Thin-Layer Chromatography The following Catalog #s are recommended: ALUMINA A-TLC, 1kg ALUMINA A-TLC, 50kg ALUMINA B-TLC, 1kg ALUMINA B-TLC, 50kg ALUMINA N-TLC, 1kg ALUMINA N-TLC, 50kg ALUMINA G-TLC, 1kg ALUMINA G-TLC, 50kg Deactivation Protocols Introduction SAI's Aluminas are unique products, i.e. Super I, Std Act I, etc. High activity Alumina can be used for polar samples in nonpolar solvents, and for the purification of

4 solvents. Lower activities of Alumina can readily be obtained by the addition of polar media, especially water. Thus each problem can be discretely resolved via the adjustment of each sorption system. Special Features Super I Aluminas show an approximate double capacity as compared to Activity I. Super I does not have to be deactivated in steps. By following the appropriate deactivation curves, deactivation can be achieved in minute increments. Deactivation behavior By following the procedures below, it is relatively easy to obtain the desired activity. Deactivation Behavior - Alumina Activity Grade Alumina Type Super I I II III IV V Super I - A,B,N % H 2 O Added Std Act I - A,B,N NA % H 2 O Added A = Acid, B = Base, N = Deactivation Procedure(s) The % water addition shown above is based upon weight / weight relationships; these relationships are critical and any deviation will/could result in obtaining improper activities. To reproduce the desired activity, weigh an appropriate amount of Alumina into a glass bottle. Add the appropriate weight of water to the Alumina and close the bottle with a stopper. For example, 97 g of Alumina + 3 g H 2 O = 3% water addition. Shake well until all lumps disappear. Wait until the mixture has cooled to room temperature. Keep the container closed so that equilibrium conditions remain constant. Application: Typical Chromatography Uses of Alumina Application ALKALOIDS Isolation from ergot-, opium-, rauwolfia-, and other alkaloids ANTIBIOTICS Isolation, purification ESSENTIAL OILS Removal of terpenes PLANT EXTRACTION Isolation of active substances DEHYDRATION OF ORGANIC SOLVENTS ENZYMES Purification GLYCOSIDES Recommended Alumina Basic, medium activity, Specialty Basic, Basic,, Acid

5 Isolation of digitalis-, strophantus-, glycosides, etc. REMOVAL OF PEROXIDES from organic solvents HORMONES Isolation and purification of synthetic products, of ketosteroids from neutral materials, etc. PURIFICATION OF ORGANIC SOLVENTS for analytical and technical purposes OILS Clarification of fatty oils, separation of fatty acids PCB's Removal from solvents, transformer oils REMOVAL OF PYROGENS from injectable solutions and infusions Basic Alumina "C" CAT#: Alumina "P" CAT#: ALUMINA C (PCB), 50kg ALUMINA P (PYROGEN), 50kg Back to Top