High Performance Polymer Chromatography Products

High Performance Polymer Chromatography Products

Quality, Innovation Competitive Price Welch Materials develops and manufactures chromatography consumables including HPLC columns, solid phase extraction (SPE) columns, GC columns, QueChERS, flash columns, sample vials, syringe filters, and chromatographic bulk media. HPLC instruments and SPE automation equipments are also in the product lines of Welch. Welch Materials was established in 2003 in both Maryland, USA and Shanghai, China. Our initial strength was our extensive experience on particle surface modification science and techniques. We are experts on bonding chemistry and innovative packing materials for chromatography applications. Through the optimal utilization of our resources across the two countries, we have developed many innovative LC and GC consumable products, in particular, our HPLC columns and SPE products. A new facility located in Zhejiang province, China will be put in use soon as the headquarter and manufacturing site of Welch Materials.

Quality, Innovation, Competitive Price Polymer Reversed Phase Chromatography Media Pore Sizes Poly(styrene-co-divynylbenzene) 3, 5, 10, 15, 20, 30, 40, 50 and 60 µm 300, 500, and 800 Å Proteins, peptides, nucleic acids, antibiotics, and low molecular weight pharmaceuticals 3µm 5µm 100 Å 300 Å 500 Å 800 Å 10µm 30µm Figure 1. Monodisperse polymer spheres with controllable particle size and pore size Mobile phase A Mobile phase B Gradient 4.6 mm I.D. x 250 mm, 10 μm, Xtimate TM PS/DVB 0.1 % TFA in water 0.1 % TFA in ACN 20 %-40 % B for 20 min 40 % B for 10 min 1 ml/min 80 %B~95 %B 10 min UV @ 280 nm Figure 2. Reproducible separation of proteins with 8 batchs Carbohydrate Analysis Chromatography Media Crosslink Degree Metal Ions Chelated Sulfonating poly(styrene-co-divynylbenzene) 3, 5, 10, 15 and 20 µm 5%, 8% and 10% Na +, Ca 2+, K + and Pb 2+ Carbohydrates and organic acids 5µm 10µm Figure 3. SEM of carbohydrate analysis chromatography media. Mobile phase Temperature Injection Volμme Sample Xtimate TM Ca, 10 µm, 8%, 7.8 300 mm Nanopure water 0.6 ml/min 85 C 10 µl ELSD 1. Melezitose, 2. Maltose, 3. Glucose, 4. Maltitol, 5. Fructose, 6. Erythritol, 7. sorbitiol. Figure 4. Analysis of Sugar mixture on carbohydrate analysis chromatography media.

Ion Exchange Chromatography Media Pore Sizes Highly crosslinked polyacrylate microspheres 10, 15, 20, 30, 40 and 50 µm 300, 500, and 800 Å Proteins, oligonucleotides, peptides, oligosaccharides Fig.5. SEM of ion exchange chromatography media Figure 6. Application of ion exchange chromatography media. Sample Eluent 1 ml, Xtimate TM Q 10 ml, 5 mg/ml BSA 20 mm Tris, 1M NaCl, ph8.0 1 ml/min AKTA Purifier (UV@280nm) Affinity Chromatography Media Pore Size Functional Group Highly crosslinked polyacrylate microspheres 30 and 50 µm 800 Å Hydrophilic layer coated and Ni 2+ chelated Histidine labeled proteins Figure 7. SEM of affinity chromatography media Sample Eluent 1 ml, Xtimate TM Ni-IDA, 50 μm 10 ml 5mg/ml BSA 20 mm Tris, 1 M NaCl ph8.0 1 ml/min AKTA Purifier (UV@280 nm) Figure 8. Application of affinity chromatography media

Ordering Information Polymer Reversed PhaseChromatography Media Xtimate TM PS/DVB Non-porous 300 Å 500 Å 800 Å Non-porous 300 Å 500 Å 800 Å 3 μm 899-00200 899-00203 899-00204 899-00205 25 μm 899-00700 899-00703 899-00704 899-00705 5 μm 899-00300 899-00303 899-00304 899-00305 30 μm 899-00800 899-00803 899-00804 899-00805 10 μm 899-00400 899-00403 899-00404 899-00405 40 μm 899-00900 899-00903 899-00904 899-00905 15 μm 899-00500 899-00503 899-00504 899-00505 50 μm 899-01000 899-01003 899-01004 899-01005 20 μm 899-00600 899-00603 899-00604 899-00605 60 um 899-01100 899-01103 899-01104 899-01105 Carbohydrate Analysis Chromatography Media Phases 5% 8% 10% Phases 5% 8% 10% 5 μm 898-11030 898-12030 898-13030 5 μm 898-41030 898-42030 898-43030 Xtimate TM H (PS/DVB-SO 3 H) Xtimate TM Na (PS/DVB-SO 3 Na) Xtimate TM Ca (PS/DVB-SO 3 Ca) 10 μm 15 μm 898-11050 898-11060 898-12050 898-12060 898-13050 898-13060 Xtimate TM K (PS/DVB-SO 3 K) 10 μm 15 μm 898-41050 898-41060 898-42050 898-42060 898-43050 898-43060 20 μm 898-11070 898-12070 898-13070 20 μm 898-41070 898-42070 898-43070 5 μm 898-21030 898-22030 898-23030 5 μm 898-51030 898-52030 898-53030 10 μm 898-21050 898-22050 898-23050 Xtimate TM Pb 10 μm 898-51050 898-52050 898-53050 15 μm 898-21060 898-22060 898-23060 (PS/DVB-SO 3 Pb) 15 μm 898-51060 898-52060 898-53060 20 μm 898-21070 898-22070 898-23070 20 μm 898-51070 898-52070 898-53070 5 μm 898-31030 898-32030 898-33030 Xtiamte TM Ca 15 μm 898-31060 898-32060 898-33060 10 μm 898-31050 898-32050 898-33050 (PS/DVB-SO 3 Ca) 20 μm 898-31070 898-32070 898-33070 Ion Exchange Chromatography Media Phases 300 Å 500 Å 800 Å Phases 300 Å 500 Å 800 Å Xtimate TM SP (Polyacrylate -SO 3 - ) Xtimate TM CM (Polyacrylate- CH 2 COO- ) 10 μm 897-10051 897-10052 897-10053 10 μm 897-30051 897-30052 897-30053 15 μm 897-10061 897-10062 897-10063 15 μm 897-30061 897-30062 897-30063 20 μm 897-10071 897-10072 897-10073 Xtimate TM Q 20 μm 897-30071 897-30072 897-30073 (Polyacrylate- 30 μm 897-10081 897-10082 897-10083 N+(CH 3 ) 3 ) 30 μm 897-30081 897-30082 897-30083 40 μm 897-10091 897-10092 897-10093 40 μm 897-30091 897-30092 897-30093 50 μm 897-10101 897-10102 897-10103 50 μm 897-30101 897-30102 897-30103 10 μm 897-20051 897-20052 897-20053 10 μm 897-40051 897-40052 897-40053 15 μm 897-20061 897-20062 897-20063 15 μm 897-40061 897-40062 897-40063 20 μm 897-20071 897-20072 897-20073 Xtimate TM DEAE 20 μm 897-40071 897-40072 897-40073 (Polyacrylate- 30 μm 897-20081 897-20082 897-20083 N+H(CH 2 CH 3 ) 2 ) 30 μm 897-40081 897-40082 897-40083 40 μm 897-20091 897-20092 897-20093 40 μm 897-40091 897-40092 897-40093 50 μm 897-20101 897-20102 897-20103 50 μm 897-40101 897-40102 897-40103 Phases 800 Å Phases 800 Å Xtimate TM Ni-IDA (Polyacrylate-Ni 2 +) 30 μm 896-11011 Xtimate TM Ni-NTA 30 μm 896-12011 50 μm 896-11031 (Polyacrylate-Ni 2 +) 50 μm 896-12031

Quality, Innovation, Competitive Price HPLC columns are listed in database Headquarter and Manufacturing Site Welch Materials (Zhejiang), Inc. 6 th floor, No.855 Xianyuan RD, Jinhua Zhejiang Province 321016, China Tel: (86) 579 82795008 Fax: (86) 579 82795006 China Domestic Marketing and Sales Center Welch Materials (Shanghai), Inc. Unit B102, 326 Edison RD, Shanghai, China Tel: (86) 21 50276760 Fax: (86)21 50276760 North America Sales Center Welch Materials, Inc. 9909 Bluegrass RD, Photomac, MD 20854 Tel:(410)998-2362 Fax:(410)998-2276 India Sales Centre Imptech Scientific. 203 Veena Appartments, Dharam Karam Road Ameerpet, Hyderabad- 500 016 A.P., INDIA Tel: (91) 040 23740443 Fax: (91) 040 23740483 Email:sales@impscience.com Email: sales@welchmat.com Website: www.welchmat.com/english