888.267.4436 techsupport@origene.com www.origene.com Name:CK8 (Keratin 8) mouse monoclonal antibody, clone OTI2E4 (formerly 2E4) Product Data Sheet - TRUEMAB Catalog: TA500022 Components: CK8 (Keratin 8) mouse monoclonal antibody, clone OTI2E4 (formerly 2E4) (TA500022) WB positive control also available: 20ug myc-ddk tagged KRT8 HEK293T over-expression lysate lyophilized in RIPA buffer (LC419425). (Reconstitute into 20ul of 1x SDS sample buffer before loading; load 5ul per lane as WB control or as desired) Amount: 100ul Immunogen: Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli. Host: Mouse Isotype: IgG1 Species Reactivity: Human Guaranteed Applications: WB, IHC, IF Suggested Dilutions: WB 1:500~1000, IHC 1:50, IF 1:50~100, Concentration: 1.46 mg/ml Buffer: PBS (ph 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Purification: Purified from mouse ascites fluids by affinity chromatography Storage Condition: Shipped at -20C or with ice packs. Upon delivery store at -20C. Dilute in PBS (ph7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. Target Target Name: Homo sapiens keratin 8, type II (KRT8), transcript variant 2 Alternative Name: Database Link: Function: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO NP_002264 Entrez Gene 3856 Human Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity
and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Validation Data HEK293T cells were transfected with the pcmv6- ENTRY control (Left lane) or pcmv6-entry KRT8 (RC209570, Right lane) cdna for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- KRT8. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-krt8 monoclonal antibody. Adenocarcinoma of Human breast tissue using anti- KRT8 mouse monoclonal antibody.(heat-induced (ph8.5) at
Human Kidney tissue within the normal limits using anti- KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, ph6.0, 100C for 10min, TA500022) Carcinoma of Human liver tissue using anti-krt8 mouse monoclonal antibody.(heat-induced epitope Carcinoma of Human lung tissue using anti-krt8 mouse monoclonal antibody. (Heat-induced epitope
Human Ovary tissue within the normal limits using antikrt8 mouse monoclonal antibody. (Heat-induced (ph8.5) at 120 Cfor 3 min, TA500022) (1:750) Adenocarcinoma of Human ovary tissue using antikrt8 mouse monoclonal antibody.(heat-induced (ph8.5) at Human pancreas tissue within the normal limits using anti-krt8 mouse monoclonal antibody. (Heat-induced (ph8.5) at 2016 OriGene Technologies, Inc. 9620 Medical Center Dr., Suite 200, Rockville, MD 20850
Carcinoma of Human thyroid tissue using anti-krt8 mouse monoclonal antibody. (Heat-induced epitope Human endometrium tissue within the normal limits using anti-krt8 mouse monoclonal antibody. (Heatinduced epitope retrieval by 1mM EDTA in 10mM Tris buffer (ph8.5) at Adenocarcinoma of Human endometrium tissue using anti-krt8 mouse monoclonal antibody. (Heat-induced (ph8.5) at
Human prostate tissue within the normal limits using anti-krt8 mouse monoclonal antibody.(heat-induced (ph8.5) at Carcinoma of Human prostate tissue using anti-krt8 mouse monoclonal antibody. (Heat-induced epitope Carcinoma of Human bladder tissue using anti-krt8 mouse monoclonal antibody.(heat-induced epitope
Anti-KRT8 mouse monoclonal antibody (TA500022) immunofluorescent staining of COS7 cells transiently transfected by pcmv6-entry KRT8(RC209570). Immunofluorescent staining of A549 cells using anti- KRT8 mouse monoclonal antibody (TA500022). * More validation images may be available on our website: http://www.origene.com/antibody/ta500022.aspx