Hamilton PRP Polymeric Columns Hamilton s Polymeric Reversed Phase (PRP) columns combine the characteristics of silica based and polymeric columns for improved versatility and longer life. With PRP columns, you can let your sample dictate the necessary separation conditions - they are not restricted to the acidic range of silica columns. PRP Columns PRP Columns Provide Enhanced Selectivity - Stable from ph 1 to 13. Acidic and basic compounds can be analyzed on the same column, by varying the ph of the mobile phase Long Column Life - Stable in any concentration of aqueous buffer or organic modifier Improved Sample Recovery - All PRP columns are made of poly (styrene-divinyl benzene) and contain no silanol groups. Silanol groups present on silica-based columns can cause irreversible adsorption and poor sample recovery High-Pressure Stability - Stable up to 340 bar (5000 psi), for high flow rates and quick analyses CRS offers the following PRP columns: Revered Phase PRP-1 - A 5 or 10 µm, 100 A packing for general use PRP-3 - A 10 µm, 300 A packing for a wide pore separation of proteins Ion Chromatography PRP-X100 - A 10 µm anion exchanger for use with organic or inorganic anions, with or without suppression PRP-X200 - A 10 µm cation exchanger for use with organic or inorganic cations, with or without suppression Carbohydrate Analysis HC-40, HC-75 - These are 10-15 µm ligand exchange, gel-type packings for the separation of carbohydrates. HC-40 is a calcium form, 4.0% cross-linked gel, while HC-75 is a calcium form, 7.5% cross-linked gel. Reversed Phase PRP-1, 15 cm x 4.1 mm ID, 5 µm 79444 PRP-1, 25 cm x 4.1 mm ID, 10 µm 79427 PRP-1 Guard Column Starter Kit (includes one holder, two cartridges) 79447 PRP-1 Guard Column Cartridges, 5/pk 79445 PRP-3, 15 cm x 4.1 mm ID, 10 µm 79466 PRP-3 Guard Column Starter Kit (includes one holder, two cartridges) 79461 PRP-3 Guard Column Cartridges, 5/pk 79454 Ion Chromatography PRP-X100, 15 cm x 4.1 mm ID, 10 µm 79434 PRP-X100, 25 cm x 4.1 mm ID, 10 µm 79433 PRP-X200, 25 cm x 4.1 mm ID, 10 µm 79442 Carbohydrate Analysis HC-75 Ca Form, 30.5 cm x 7.8 mm, 10-15 µm 79436 76 Chromatography Research Supplies, Inc.
Mobile Phase Filters/ Handling Systems The Mobile Phase Delivery Systems consists of Solvent Pickup Adapter - for inline filtration. A stopcock allows vacuum degassing after filtration HPLC Reservoir - The unique conical reservoir bottom assures nearly complete delivery of mobile phase. It is made from borosilicate glass for purity Ultra-ware 5 Valve Cap Mobile Phase System Designed for use with mobile phase recyclers. Mobile phase recirculates directly into the reservoir, while maintaining a helium spared environment. The system is designed to filter, de-gas and deliver mobile phase in one closed system. Up to five valves can be used at once, for mobile phase recirculation, pump, filtration, vacuum/vent and sparge. A removable 47 mm glass frit allows easy cleaning and replacement of membrane filters. Complete Systems Mobile Phase System, 1 L 625901 Mobile Phase System, 2 L 625902 Mobile Phase System, 5 L 625905 625902 Ultra-ware 4 Valve Cap Mobile Phase System This 4 valve cap system allows filtering, degassing and delivery of your mobile phase with one apparatus, without removing the cap and without transferring to another reservoir. Complete Systems Mobile Phase System, 1 L 620901 Mobile Phase System, 2 L 620902 Mobile Phase System, 5 L 620905 Replacement Parts and Filters 620902 Replacement Parts (for parts not listed, please call) HPLC Mobile Phase Reservoir, 1 L 620911 HPLC Mobile Phase Reservoir, 2 L 620912 HPLC Mobile Phase Reservoir, 5 L 620915 Glass Frit Filter Support, 47 mm 620920 HPLC Solvent Pickup Adapter 620921 47 mm Membrane Filters Nylon, 0.2 µm, 100/pk 182047 Nylon, 0.45 µm, 100/pk 185047 PTFE, 0.2 µm, 100/pk 182048 PTFE, 0.5 µm, 100/pk 185048 www.chromres.com 77
Mobile Phase Filters / Fittings HPLC Mobile Phase Filter Assembly, Ultra-ware This is a low cost, easy-to-use microfiltration system which consists of a straight-wall funnel, fritted support base, inner joint flask and an aluminum clamp. The support base has a coarse porosity glass frit and integral vacuum connection. The assembly uses 47 mm filters. The vacuum connection is positioned above the filtrate drip tip to prevent contamination of the vacuum line from solvent splattering. TS 40/35 joints. Complete Systems Funnel Cap, 300 ml/ Flask Cap 1000 ml 620931 Funnel Cap, 500 ml/ Flask Cap 2000 ml 620932 620931 Replacement Parts Funnel, Graduated, 300 ml 620933 Funnel, Graduated, 500 ml 620935 Fritted Glass Support Base, TS 40/35 620936 Flask, 1000 ml, TS 40/35 620937 Flask, 2000 ml, TS 40/35 620938 Aluminum Clamp, 47 mm 620940 Valco Nuts These can be used with Valco fittings as well as most other manufacturers. Nuts are made from stainless steel. Size Standard Nut 1/16 ZN1 Long Nut 1/16 LZN1 Valco Ferrules Ferrules are made of stainless steel and can be used with metal or PTFE tubing. The polyimide ferrules can be used up to 350 O C. Order as many ferrules as you want; they are packaged together in a bag. Size Material Ferrule 1/16 300 Series Stainless ZF1 1/16 Polyimide ZF1V 78 Chromatography Research Supplies, Inc.
HPLC Fittings / Accessories Replacement Column Frits - For 4.6 mm ID (1/4 OD) Columns Made from stainless steel or PEEK With PEEK sealing rings or without All 1.6 mm thick Qty. Frit, SS without Sealing Ring, 0.5 µm 10 624400 Frit, SS without Sealing Ring, 2 µm 10 624410 Frit, SS with Sealing Ring, 0.5 µm 10 624420 Frit, SS with Sealing Ring, 2 µm 10 624430 Frit, PEEK with Sealing Ring, 0.5 µm 2 624421 Frit, PEEK with Sealing Ring, 2 µm 2 624431 SS Frits, With or Without Sealing Rings PTFE Tubing Pure PTFE tubing has many low-pressure fluid transfer uses. It is ideal for HPLC solvent lines. 10 Feet 50 Coil 1/16 x 1/32 430025 430026 1/8 x 1/16 430035 430036 1/4 x 3/16 430045 430046 PTFE Tubing Terry Tool Tubing Cutter This is a uniquely-designed precision tubing cutter that cuts any 1/16 OD metal tubing. Its compact design allows use in awkward, hard-to-reach places without removing tubing from the HPLC. Terry Tool Tubing Cutter 460008 Spare Cutting Wheel 460009 460008 PEEK Tubing - 1/16 OD, 5- Foot Lengths Manufactured to precise tolerances Color-coded for easy identification Biocompatible Color Maximum Pressure 0.005 ID Red 340 bar / 5000 psi 624305 0.007 ID Yellow 340 bar / 5000 psi 624307 0.010 ID Blue 340 bar / 5000 psi 624310 0.020 ID Orange 340 bar / 5000 psi 624320 0.030 ID Green 204 bar / 3000 psi 624330 0.040 ID Natural 34 bar / 500 psi 624340 PEEK Tubing www.chromres.com 79
Guard Column, Tubing HPLC Guard Column This is an easy-to-use, effective guard column system for your HPLC. It protects against contamination and pressure rise by column plugging. Guard column cartridges are replaced as needed simply by unscrewing the housing and placing in a new cartridge. Cartridges are 1 cm in length with 3 mm ID, and are available with different packings to match exactly the packing of the analytical column you are already using. All packings are 5 micron. HPLC Guard Column System Cartridges, 2/pk C8 (octyl) 620201 C1 (methyl) 620701 Phenyl (note: 7 micron) 620302 CN (cyano) 620401 NH2 (amino) 620501 Silica 620601 Pre-cut Stainless Steel Tubing, 1/16 OD Our pre-cut tubing is ready for immediate installation; it is much easier to work with than bulk tubing. The tubing is cut electrochemically and then electropolished, ensuring square, burr free ends without flaring. Tubing is then cleaned with solvent wash and further steam-cleaned using deionized water, for removal of both organic and ionic contaminants. Pre-cut tubing is made from 316 grade stainless steel. 2 pieces each. ID 5 cm 10 cm 20 cm 30 cm 0.005 T5C5D T10C5D T20C5D T30C5D 0.010 T5C10D T10C10D T20C10D T30C10D 0.020 T5C20D T10C20D T20C20D T30C20D 0.030 T5C30D T10C30D T20C30D T30C30D 0.040 T5C40D T10C40D T20C40D T30C40D Pre-cut Stainless Steel Tubing Bulk Stainless Steel Tubing - Minimum tubing order is 5 feet Premium 316 stainless steel tubing for all HPLC applications. Pre-cleaned. OD x ID Per Foot 50 Coil 200 Coil 1/16 x 0.010 430001 430002 430003 1/16 x 0.020 430004 430005 430006 1/16 x 0.030 430008 430010 430011 1/16 x 0.040 430012 430013 Bulk Stainless Steel Tubing 80 Chromatography Research Supplies, Inc.
HPLC Fittings EZ-Tite TM Fittings Wrench Free Resist Cracking, Stripping and Leakage Biocompatible Materials These wrench free fittings are precision manufactured from polymeric materials for maximum reliability and ease of use. Unlike molded fittings, these machined parts resist cracking, breaking, thread stripping and leakage in both low and high pressure applications. They are made from biocompatible materials for a broader range of applications. 10-32 threads. 10/pack Material Ref. Upchurch No One-Piece Fitting PEEK F120X 624100 Two-Piece Fitting Delrin, PEEK ferrule F200X 624111 Replacement Ferrules PEEK F142X 624130 EZ-Tite Fittings Inline Filters / Precolumn Filters Stainless Steel or PEEK Economical Protection for your Injector and HPLC Column Compatible with all HPLC Systems Protect your sample injector valve from particles shed through normal pump piston seal wear, and your column from premature plugging. Inline Filters - The filter holder is available in 316 stainless steel or PEEK. The 2 µm replaceable filter element provides effective protection without increasing system back pressure. Inlet and outlet connectors are industry standard 1/16 connections. The PEEK filter has a hand tight design that requires no wrenches. Precolumn Filter - The precision manufactured 316 stainless steel holder incorporates a through-hole and dispersion cone geometry for minimal band spreading. Inlet and outlet connections are industry standard 1/16. One-Piece Fitting Two-Piece Fitting Ref. Upchurch No. Inline Filter SS with 2 µm SS Frit A-314 624200 Replacement Inline Frit, SS, 2 µm, 10/pk A100X 624230 Inline Filter PEEK with 2 µm PEEK Frit 624201 Replacement Inline Frit, PEEK, 2 µm, 2/pk 624431 Precolumn Filter SS, 0.5 µm Frit A-316 624210 Replacement Precolumn Frit, SS, 0.5 µm, 10/pk A-103X 624250 Inline Filters www.chromres.com 81