Photopette German Engineered

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Photopette German Engineered Ultra Fast Handheld Spectrophotometer Handheld Smart Device High Accuracy & Repeatability <2 seconds per Measurement No Cross-Contamination 25,000+ Measurement per Charge

Introducing Photopette The Photopette device is a fast, accurate and economically priced fixed wavelengths handheld spectrophotometer universally used in life-sciences, environmental surveillance, food & beverages and chemical industry to measure concentrations in liquid samples. The Photopette personal photometer offers the convenience, performance and seamless integration with cloud, web and mobile computing expected from contemporary devices and applications, saving users precious sample and time, whilst still producing precise and accurate results. The device enables users to dramatically improve the workflow and increase productivity through speed and portability. ne Click ne Result ne Second Key Features High Speed Ultra-fast measurements (less than 2 seconds for each measurement) Immediate sampling, no device warm up required Intuitive exportation of results via email Instant on-spot quantification of nucleic acids, proteins, cells density and many more Optical System Patented optical system Measurement precision ±0.02 AU >500,000 measurements per wavelength Low energy consumption (25,000+ measurements per charge) No maintenance or lamp change required Simple and Safe Handheld with small footprint Ergonomic Disposable CuveTip (no cross-contamination) No transfer or transport of sample Fast and simple to use, no training required Advanced Software Application Geo tagging, photo, notes, date and time embedded in data file Real-Time data sharing Send measurement data instantly or link to cloud storage Automatic software and firmware updates 01

Photopette Workflow ULTRAFAST Measure & Share in 4 Steps Select Measure Analyse Share Choose your Application or Wavelength Perform Qualitative & Quantitative Measurement using Photometry Observe Protein, DNA, RNA, Cell-Count and more... Share your Results with Ease to other Devices or People in Real-Time Advanced Software Application Inbuilt applications selectable by user Automatic Time Stamp, Geotag, Photo and Notes Forward data via email or data storage in cloud Method Maker function for User defined Calibrations 02

Optical System The Photopette optical system is a multicomponent, ultra fast handheld device that integrates a photodiode detector with light emitting diodes (LEDs). It also has an in-build environmental light correction and can be used in the lab environment and outdoor without shielding environmental light from the sample. Within the Photopette, a light emission fiber transmits light from the LEDs to the CuveTip and a light collection fiber collects light from the CuveTip to the detector. Light emission Lens LEDs Optical module holder Photodiode Light to collection fiber Mirror coated CuveTip Cover Fiber holder The injection moulded CuveTip is coated with a mirror coating to reflect the light back through the sample. The curvature of this mirror surface, located at the very end of the measurement tip, is optimized to reflect most of the light back into the collecting optical fiber through which the signal is transmitted to the photodiode detector. Fibers CuveTip Optical bundle A dual-purpose disposable reflective tip was designed to act as a sample holder and a light-reflecting system, which is in stark contrast to the operation of mainstream spectrophotometers and photometers. Small volume analytes may be measured with low sample loss and no sample transfer using this proprietary CuveTip. A minimum volume of 40 µl is required to fill the cavity of the CuveTip. This volume is recovered after the measurement. CuveTip Cavity CuveTip Mirror 03

Applications The Photopette is a high performance portable handheld Spectrophotometer for fast and demanding applications Cell Culture Allow fast measurement in the cell culture hood Absolute quantification of cell density through calibration curve Mammalian, bacterial or yeast cell count Cell viability test using Resazurin dye Enzymatic assays for metabolites Nucleic Acid and Protein Quantification DNA, RNA and Oligo concentration determination Bradford Assay Direct protein concentration Enzymatic Assays * Using 600 nm - Cell Density NAD/NADH based enzymatic assays Lactate, pyruvate, glucose & alcohol, and more... Hemocytometer 8.0 N.S Photopette Cell Density (10 6 cells/ml) 6.0 4.0 2.0 N.S 0.0 Sample A Sample B * Using 260 nm - DNA Quantification 30 Microvolume Spectrophotometers Photopette DNA Concentration (µg/ml) 20 10 0 Sample A1 Sample B1 Sample A2 Sample B2 * Using 280 nm - Direct Protein Quantification 2.0 Microvolume Spectrophotometers Photopette Protein Concentration (mg/ml) 1.5 1.0 0.5 0.0 Sample A Sample B * Shi-Hao Li, Abhinav Jain, Timo Tscharntke, Tobias Arnold, and Dieter W. Trau (2018). Hand-Held Photometer for Instant On-Spot Quantification of Nucleic Acids, Proteins, and Cells. Journal of Analytical Chemistry, 90(4), 2564 2569. 04

Designed for: The Photopette is the ideal instruments for quick measurements at the lab bench, in the cell culture hood or out in the plant or on the field. Academic teaching laboratories (life science) Cell culture or tissue culture DNA and Protein quantification Beer, wine and spirits Farming and livestock Outdoor water and chemical analysis In Process QC testing (Photopette is for research use only not approved for human or veterinary clinical diagnostics) Outdoor In Sterile Environment Product available: In Brewery Model Photopette Cell Photopette Turbidity Photopette Custom / OEM Wavelengths 340 nm, 570 nm, 600 nm 570 nm, 860 nm Custom Wavelengths ios and Andriod Apps Ergonomic Photo, Notes, Timing, GPS Embedding Weight 160 g Language English, German and Chinese Applications - Enzymatic Assays - Cell Viability Assays - Cell Density - Compatible to USEPA 180.1 and ISO 7027 - Custom Applications 05

Technical Specifications Instrument Type Wavelength Accuracy Measurement Precision & Accuracy Photometric range Measurement time Warm up time Lifetime (light-source) Operation and handling Operating conditions Storage conditions Disposable CuveTip chemical compatibility Power supply Dimensions and weight Supported languages (App) Certification Fixed wavelengths handheld spectrophotometer with ambient light compensation ±2 nm better than ±0.020 AU 0.000 AU ~ 3.000 AU Within 2 seconds per measured wavelength None >500,000 measurements per wavelength Easy sterilization with ethanol wipes Water and dust resistant housing Resistant to mild acids and solvents Recommended: 18~25 C at 30% RH Maximum: 5~45 C at 80% RH (non-condensing) (25,000 measurements and / or 6 days standby on a single charge) Auto power save mode -20 to 50 C (max) and up to 80% RH (non-condensing) Standard CuveTip ; UV CuveTip Sterile *optional Aqueous solutions, dilute acids and bases, alcohols, vegetable oils, blood serum, cell culture media, etc Internal 2500 mah battery with microusb charging socket Fits within 202 mm x 47 mm x 42 mm; Weight = 160 g approx English, German and Chinese CE and FCC by TÜV SÜD Singapore Photopette App is now available at Ordering Information 500008-PC 500008-PT 500010-6BX-STAN 500010-12BX-STAN Photopette Cell Kit supplied with Photopette device (supports 340 nm, 570 nm & 600 nm), Photopette stand holder, CuveTip Box, CuveTip Ejector Box, 88 disposable CuveTips, USB connection cable and quick start guide Photopette Turbidity Kit supplied with Photopette device (570 nm & 860 nm), Photopette stand holder, CuveTip Box, CuveTip Ejector Box, 88 disposable CuveTips, USB connection cable and quick start guide Standard Disposable CuveTips - 6 x racks per Carton Bulk Standard Disposable CuveTips - 12 x racks per Carton 06

Represented by All the information within this brochure was correct at the time of printing. Tip Biosystems reserves the right to make changes to their products to improve their performance or appearance. Tip Biosystems Pte Ltd 21 Bukit Batok Crescent, #14-79 WCEGA Tower, Singapore 658065 +65-6914 7600 sales@tipbiosystems.com www.tipbiosystems.com * Photopette and CuveTip are registered trademarks of Tip Biosystems Pte Ltd, Singapore. 0318-SG-0300