What are Genetically Modified Crops and how are they made? Professor Idah Sithole-Niang Department of Biochemistry, UZ GMASSURE - UZ

What are Genetically Modified Crops and how are they made? Professor Idah Sithole-Niang Department of Biochemistry, UZ GMASSURE - UZ

Outline Definitions Concept of Genetic Modification Agricultural context Why biotech? Challenges/Opportunities Status & specific opportunities in Africa Non-GM products

Definitions Biotechnology is the use of biological systems to create goods and services Gene manipulation Genetic engineering Genetic modification Transgenesis LMOs: living modified organisms GMOs: genetically modified organisms

Concept of Genetic Modification through plant transformation Identify genes of interest for a trait (eg. insect resistant genes) Insert into an organism of interest (eg. Insert insect resistance gene into a susceptible plant to make it resistant) very common with crop plants Genes can come from a variety of sources: From the same plant species From wild relatives From another crop From bacteria or another organism

Agricultural context Cross breeding and induced mutation are important tools of plant breeding, they also have limitations: 1. Cross breeding only works between related plants. 2. For some species cross breeding is extremely difficult. 3. Cross breeding can take very long. 4. Linkage drag 5. Mutation is very undirected and unpredictable, HORIZONS sprl

Genetic Modification of Plants Traditional plant breeding x elite variety Related variety Genetic Modification any gene source HORIZONS sprl

Genetic Modification of Plants Technical advantages: Specific Faster Possible with plants that do not cross sexually Much greater reservoir of genes HORIZONS sprl

Generating Varieties of Agricultural Plants and Animals Conventional breeding Crossing of two varieties with useful characteristics Hybrids Tissue culture Cloning Chemical mutagenesis Irradiation In vitro fertilization Artificial insemination

Using Radiation to Create Mutant Varieties of Plants Gamma Rays X Rays

Breeders, geneticists, and genetic engineers work with genes Genes carry the information that is passed from generation to generation. They are the blueprint of life Anything that is alive has genes. The information of genes is carried in the form of DNA molecules. The language of DNA, is the same regardless of the organism A key point for genetic engineering DNA molecule

Breeders, geneticists, and genetic engineers work with genes Genes carry the information that is passed from generation to generation. They are the blueprint of life DNA molecule

Breeders, geneticists, and genetic engineers work with genes Anything that is alive has genes. The information of genes is carried in the form of DNA molecules. DNA molecule

Breeders, geneticists, and genetic engineers work with genes The language of DNA is spelt out in a simple alphabet of 4 letters A, C, G, T DNA molecule

Breeders, geneticists, and genetic engineers work with genes The language of DNA, is the same regardless of the organism A key point for genetic engineering DNA molecule

You and Your DNA How much of your DNA sequence do you share with a banana? A: None B: 1% C: 10% D: 50% E: 90% Do you know that humans share about 50% of their DNA with a banana! Good grief!: I m a banana! Sigma ad 8/2008

Getting genes into cells plant transformation 2. Put the engineered gene into cells of the desired plant

Step X step Dr. Mufandaedza

Regulation of GM crops moves through different stages of crop development and deployment General release Full safety assessment Confined Field Trials Lab Growth Chamber or greenhouse CFT Application General Release Application Approval

1. Why biotechnology? World population will reach 9 billion by 2040 Some undernourished either in terms of both quantity or quality Arable land shrinking due to erosion, pollution, and other forms of land use Increasing shortage of fresh water for drinking and irrigation Climate change will increase the need for keeping up with well adapted crops

2. Why biotechnology? Increasing demand for fuels and chemicals from renewable sources as oil reserves become depleted and oil-based commodities more expensive 80% of world caloric intake comes from only 4 crops Not a silver bullet but can contribute significantly to finding solutions to these challenges

1.Challenges Need to produce: More crops per hectare More crop per liter of water Marginal- Arid- or Saline land

2. Challenges Enhance: Nutritional value of crops Crop diversity Reduce: Dependency on pesticides & fertilizers Post-harvest losses during storage & transport Soil erosion

Do these challenges apply to Zimbabwe? What are the Opportunities'?

1. Opportunities/pipeline Biotic stress: Disease resistance Fungal resistance Banana/Black sigatoka Virus resistance banana, cassava, yam, papaya, groundnut, sweetpotato & tomato Bacterial resistance rice, cassava, banana & potato Pest resistance/field & storage

2. Opportunities/pipeline Abiotic stress tolerance Drought tolerance maize (WEMA), wheat rice sorghum etc Salinity tolerance- maize, wheat, tobacco, sorghum, rice etc (NUWEST)

3. Opportunities/pipeline Enhanced nutrition: rice, cassava & sorghum (pro-vitamin A, Fe, Zn, Vit E) Banana- pro-vitamin A & Fe, wheat- Fe Maize, potatoes, sorghum & cassava (protein quality). Mustard pro-vitamin A Others: reducing cyanogenic compounds in cassava & changing available P by reducing phytates

You mean there are all these wonderful Opportunities!!!!?? Why the controversy? What are the issues?? Mr Dhlamini

Any Good examples in the BRICS? BRAZIL, CHINA & INDIA Prof CJC

What would it feel like if the DR & SS had $1 billion budget? What would it feel like if the NBA reviewed, 14 applications annually???

Whos Who In Africa? and where are they with their regulation? Dr. Mufandaedza

Developing high quality Pod-borer-resistant cowpea varieties Problem Insect damage in the field and in storage Losses can be up to 80%. Frequent insecticide sprays required Host plant resistance is a low-cost and environmentally friendly control measure for the farmer Figure 7. Pod damage by M. vitrata Product High yielding cowpea varieties with increased resistance to insect pests - Bt-Cowpea (Pod borer- Resistant Cowpea)

Resistance to Banana Bacterial Wilt for East African Highland Bananas Constraint: Banana bacterial wilt (BBW) disease caused by the bacterium Xanthomonas campestris pv. musacearum (Xcm)

Water Efficient Maize for Africa Africa drought-prone Maize is the most widely grown staple crop in Africa affected by drought (WEMA) In 2003 WFP spent $0.57b on food emergency due to drought in Africa Recorded droughts between 1971 and 2000, and the number of people affected

Drought tolerant Maize

Is there a drought in Zimbabwe?? Do some of these solutions apply??

Confined Field Trial to Evaluate Transgenic Cassava for Resistance to Cassava Mosaic Disease - D3/2/NBC/4/08 Susceptible (without the gene) Gene silencing line (with gene to control CMD)

Symptoms of CBSD at Harvest: CBSD Study Symptomless leaves and stems of best test line (1-718-001) Severe streaking and dieback on control line without gene

What will you have for dinner???

Symptoms of CBSD at Harvest: CBSD study Best test line; No rotting of the storage roots Control line without the gene; severe rotting of roots

Eggplant (Brinjal or Talong) Fruit and Shoot Borer This damage is prevented by Bt.

Bt cotton CFT in Malawi 2013 Mr. Mhandu

Biotech crops on trial in Africa RSA: potatoes, sugarcane, maize WEMA : RSA, Kenya, Uganda Kenya: cotton, maize, SP, cassava Egypt: cotton, potato, wheat, cucumber, melon Uganda: banana, cotton,cassava,maize, rice Nigeria - cowpea, cassava, sorghum Burkina Faso: cowpea, rice Malawi cotton, cowpea, banana

New Breeding Technologies Non- GM Technologies Need we even dream???

Non-GM: Genome editing technologies CRISPRs: clustered regularly interspaced short palindromic repeats ZFNs: Zinc finger nucleases TALENs : transcription activator-like effector nucleases

Zinc Finger Nucleases Successfully used to introduce herbicide tolerance = WEED CONTROL

TALENs Used to delete and cut out a gene in rice that confers susceptibility to bacterial blight disease

CRISPRs Chinese Academy of the Sciences developed a powdery mildew-resistant wheat through advanced gene editing Example of modification of a food crop without using insertion foreign genes

Three Requirements for growth of GM crops in Africa Political will and support from lead countries, governments and institutions Establishment of responsible and efficient regulatory systems, that are appropriate for Africa given the limited resources Communication with Society transparently and accurately

Thank you for your attention

Acknowledgments Clive James, ISAAA Cholani Weebadde, Michigan State University Peter Davies, Cornell University Program for Biosafety Systems African Biosafety Network of Expertise GMASSURE African Agricultural Technology Foundation

Current Status of Genetically Engineered Crops Grown in over 28 countries around the world by over 18 million farmers 420 million acres in 2013 Largest in acreage US (171 million acres) Largest in terms of farmers India and China 14 million farmers Types of products maize, soybeans, cotton, canola, sugar beets, alfalfa, squash, papaya Types of traits built in pesticides, resistant to herbicide, resistant to virus For the first time in 2012, developing countries grew more, 52%, of global biotech crops in 2012 than industrial countries at 48%

Drought and African Agriculture The WFP spent $0.565B of food emergency to respond to drought in sub-saharan Africa (SSA) in 2003 Over 95% of cropland in SSA is rain-fed and will remain so in the near future The risk of drought prevents investment in improved agricultural products Yield stability is key to unlock the value of basic inputs (Slide source: Dave Songstad, Monsato) Recorded droughts between 1971 and 2000, and the number of people affected