Permathene Ltd Attn: Kathy Yang PO Box 71015 1348 Auckland NEW_ZEALAND 30/12/2010 Dear Kathy, Please find the attached report to AS/NZS 4020:2005 for Permaliner FPP Black submitted for testing. Should you have any enquiries about the report or any other matters pertaining to the Standard please contact the laboratory on 61 8 7424 1512 Yours sincerely, Michael Glasson Product Testing Team Leader
Report Information Report ID : 79060 Submitting Organisation : Account : AWQC Reference : Project Reference : Product Designation : Composition of Product : Product Manufacturer : Use of Product : Sample Selection: Testing Requested : Product Type : Samples : Project Completion Date : 00120894 : Permathene Ltd 141628 : Permathene Ltd 141628-2010-CSR-1 : Prod Test: Permaliner Flexible Polypropylene PT-1398 Permaliner FPP Black FPP Resin and Carbon Black. Plastika Kritis, Iraklion - Crete, GREECE. In-Line/Geomembrane for Liquid Containment. As provided by the submitting organisation. AS/NZS 4020:2005 TESTING OF PRODUCTS FOR USE IN CONTACT WITH DRINKING WATER Composite Samples were prepared and controlled as described in Appendix A of AS/NZS 4020:2005 Extracts : Extracts were prepared as described in Appendix C, D, E, F, G, H. Project Comment : 30-Dec-2010 The results presented herein demonstrate compliance of Permaliner FPP Black to AS/NZS 4020 when exposed at area to volume ratios up to 15000 mm2/l at Product range to include 300 mu to 1.5 mm. (Roll Code # 2102702299 and Batch # 14076). PLEASE NOTE THAT THIS REPORT SHALL NOT BE REPRODUCED EXCEPT IN FULL THE RESULTS STATED IN THIS REPORT RELATE TO THE SAMPLE OF THE PRODUCT SUBMITTED FOR TESTING. ANY CHANGES IN THE MATERIAL FORMULATION, PROCESS OF MANUFACTURE, THE METHOD OF APPLICATION, OR THE SURFACE AREA-TO-VOLUME RATIO IN THE END USE, COULD AFFECT THE SUITABILITY OF THE PRODUCT FOR USE IN CONTACT WITH DRINKING WATER Michael Glasson Page 1 of 8
Summary of APPENDIX RESULTS C D E F G H Taste of Water Extract Appearance of Water Extract Growth of Aquatic Micro-organisms Cytotoxic Activity of Water Extract Mutagenic Activity of Water Extract Extraction of Metals Summary Comment : Page 2 of 8
CLAUSE 6.2 Test Information Taste of Water Extract ml volumes of 50 hardness water. Taste of Water Extract (Appendix C) Not detected. The product passed the requirements of clause 6.2 when tested at an exposure of 15000 2. Peter Christopoulos Page 3 of 8
CLAUSE 6.3 Appearance of Water Extract ml volumes of 50 hardness water. Appearance of Water Extract (Appendix D) Test (- Blank) Maximum Allowed Units Colour <1 5 HU Turbidity <0.1 0.5 NTU The product passed the requirements of clause 6.3 when tested at an exposure of 15000 1. Joanne Clark Page 4 of 8
CLAUSE 6.4 Inoculum Growth of Aquatic Micro-organisms ml volumes of test water. Growth of Aquatic Micro-organisms (Appendix E) The volume of the inoculum was 100 ml Mean Dissolved Oxygen Control 7.4 Mean Dissolved Oxygen Difference Positive Reference 6.0 Negative Reference 0.4 Test 0.10 The product passed the requirements of clause 6.4 when tested at an exposure of 15000 1. Stephanie Semczuk Page 5 of 8
CLAUSE 6.5 Cytotoxic Activity of Water Extract ml volumes of 50 hardness water. Cytotoxic Activity of Water Extract (Appendix F) Non-cytotoxic. The product passed the requirements of clause 6.5 when tested at an exposure of 15000 1. The test extracts and blank extracts were used to prepare nutrient growth medium and subsequently used to grow a cell line (ATCC Number CCL 81) in the analysis. In addition zinc sulphate (0.4 mmol) was used for the positive control in the analysis. Brendon King Page 6 of 8
CLAUSE 6.6 Bacteria Strain Mutagenic Activity of Water Extract ml volumes of 50 hardness water. Mutagenic Activity of Water Extract (Appendix G) Number of Revertants per Plate Salmonella typhimurium TA98 S9 - Blank Sample Extract Positive Controls 24, 43, 40 41, 40, 36 2517, 2413, 2273 35.7 ± 10.2 39.0 ± 2.6 2401.0 ± 122.4 NPD (20mg) + 29, 23, 20 24.0 ± 4.6 31, 29, 23 27.7 ± 4.2 2505, 2144, 2230 2293.0 ± 188.6 2-AF (20mg) Salmonella typhimurium TA100-225, 182, 194 200.3 ± 22.2 138, 134, 128 133.3 ± 5.0 1076, 1001, 1035 1037.3 ± 37.6 Azide (1.0mg) + 148, 128, 120 132.0 ± 14.4 106, 116, 132 118.0 ± 13.1 1425, 1365, 1464 1418.0 ± 49.9 2-AF (20mg) Salmonella typhimurium TA102-573, 390, 467 476.7 ± 91.9 524, 557, 485 522.0 ± 36.0 1782, 1556, 1586 1641.3 ± 122.7 Mitomycin C (2mg) + 434, 441, 415 430.0 ± 13.5 494, 419, 342 418.3 ± 76.0 Comments S9 was used as a metabolic activator. NPD (4-nitro-o-phenylenediamine), Azide, and Mitomycin C are specific positive controls for strains TA98, TA100 and TA102 respectively while 2 - AF (2-aminofluorene) when used in conjunction with S9 is a positive control for both TA98 and TA100 The product passed the requirements of clause 6.6 when tested at an exposure of 15000 1. Peter Christopoulos Page 7 of 8
CLAUSE 6.7 Method of Analysis Extraction of Metals ml volumes of 50 hardness water. Extraction of Metals (Appendix H) All methods used to determine concentrations of metals are based on those described in the 21st edition of Standard Methods for the Examination of Water and Wastewater published by the APHA, AWWA and WEF (2005). The methods have been adapted for the instrumentation in use at the Australian Water Quality Centre. Concentration of the metals described in Table 2 of the AS/NZS 4020:2005 are determined as follows: Antimony, Arsenic, Barium, Cadmium, Chromium, Copper, Lead, Mercury, Molybdenum, Nickel and Selenium by inductively coupled plasma mass spectrometry. Silver by graphite furnace absorption spectrophotometry (Varian). Final Extract Antimony Arsenic Barium Cadmium Chromium Copper Lead Mercury Molybdenum Nickel Selenium Silver Limit of Reporting 0.0005 0.0003 0.0005 0.00003 0.002 Blank <0.0005 <0.0003 <0.0005 0.00006 <0.00003 Test 1 Test 2 <0.0005 <0.0005 <0.0003 <0.0003 <0.0005 <0.0005 <0.00003 <0.00003 <0.00003 <0.00003 Max Allowed 0.003 0.007 0.7 0.002 0.05 2.0 0.01 0.001 0.05 0.02 0.01 0.1 The product passed the requirements of clause 6.7 when tested at an exposure of 15000 1. Dzung Bui Page 8 of 8