SPRIworks Systems Fully automated library construction systems with built-in size selection and cleanup Push button. Walk away. BR-15981B
SPRIworks Technology Fully automated fragment library construction systems Choose the Sample Prep System That is Right for Your Lab History of Trust: Sample Prep for Genomic 2010 - Beckman Coulter utilizes SPRI chemistry for next generation sequencing sample preparation with the launch of SPRIworks Fragment Library Systems. 2005 - Recognizing the value of SPRI chemistry optimized on Biomek automated workstations, Beckman Coulter acquires Agencourt Bioscience. 2003 - As a result of the high quality sequencing data produced by Agencourt, the NHGRI designates Agencourt as one of only five Large-Scale Centers and the only commercial center responsible for genome sequencing of organisms of high biomedical importance. SPRI technology, robotics and bioinformatics enable Agencourt to be acknowledged by the sequencing community as one of the preeminent sequencing facilities. Low-Medium Throughput (Up to 30 libraries/day) SPRIworks Fragment Library Systems Fully automated library construction with built-in size selection and enzymatic cleanup Process up to 30 samples per day Bench-top system Less than 5 minutes hands-on setup time Prepares samples for Illumina*, Roche* and Life Technologies* next generation sequencers Beckman Coulter SPRIworks vs Manual Processing Methods Manual Up to 8 SPRIworks Up to 30 High Throughput (Up to 96 libraries/day) SPRIworks HT System Fully automated library construction, with built-in size selection and enzymatic cleanup Process up to 96 samples per day SPRIworks chemistry automated on Biomek robotic platforms Less than 30 minutes hands-on preparation time Prepares samples for Illumina next generation sequencers Includes optional methods for q setup normalization and pooling SPRIworks HT Up to 96 2001 - Due to SPRI s flexibility, ability to produce high quality nucleic acid, and ease of automation, Agencourt launches sequencing services and genomics products based on SPRI chemistry. (optional) Covaris E-series can be integrated on Biomek FX P 1995 - Agencourt recognizes the advantages of magnetic bead technology and acquires the license for SPRI paramagnetic beads. SPRI is immediately used as the platform for all products and services. Fully Library Construction Library construction is the number one bottleneck in the sample preparation process for next generation sequencing, requiring significant hands-on time. Additionally, multiplexing capabilities are putting strain on laboratories to prepare more libraries for sequencing than ever before. SPRIworks and SPRIworks HT Fragment Library Systems eliminate manual library construction and provide a significant boost to productivity, reproducibility and consistency. Set-up q Manual 1-8 fully sized per person Set-up q 1-30 fully sized Set-up q 1-96 fully sized (optional) T-robot on Biomek FX P with method suite By automating sample processing, laboratories can now maximize the return on their sequencing investments while freeing up valuable resources to focus on data analysis and biological interpretation. with method suite
SPRIworks Fragment Library Systems I, II and III For Illumina, Roche and Life Technologies next generation sequencers Free Your Lab From the Constraints of Next Generation Sample Prep Built-in SPRI and Cleanup Chemistry Increase library construction throughput without running gels, cutting bands for DNA fragment size selection or using columns for cleanup. The Beckman Coulter SPRIworks Fragment Library Systems improve laboratory productivity and liberate scientists to spend time focusing on data analysis. Run up to 10 samples at a time Maximize sequencing run capacity by multiplexing up to 10 samples in parallel on each system No more manual size selection gels, cleanup columns or manual fragment library construction. Fully automated library construction process Reproducible and consistent library output Only 5 minutes of hands-on time SPRI-TE Nucleic Acid Extractor Reagents predispensed in a sealed cartridge Simple library construction process No more labor intensive pipetting 200-400 size selection 300-600 size selection Short and long fragment sizes isolated using SPRIworks Fragment Library System I for Illumina GA Sequencers and analyzed using Agilent* High Sensitivity DNA Chip. Cartridge Method Card Multi-platform Library Construction Not only does the SPRIworks bench-top instrument occupy a small footprint, but it prepares fragment libraries for Illumina, Roche and Life Technologies next generation sequencers. System I for Illumina Sequencers System II for Roche Sequencers System III for Life Technologies Sequencers Throughput Up to 10 samples in about 5 hours Up to 10 samples in under 4 hours Up to 10 samples in under 4 hours SPRIworks Fragment Library Systems have three components; The SPRI-TE Nucleic Acid Extractor, a method card that controls liquid handling, and a cartridge containing all the reagents required to prepare a single library. Basic Operation Steps SPRIworks systems are simple and easy to operate. Hands-on time is significantly decreased so that technical expertise can be allocated to experiment design and data analysis. DNA input amount Size selection ranges Supported input sample types 3-5 µg 200-400 bps 300-600 bps 500 ng 350+ 500+ 1 µg (3 µg for 150-250 size selection) 150-250 bps 150-350 bps Sheared DNA, cdna, amplicons Sheared DNA, cdna, amplicons Sheared DNA, cdna, amplicons Step 1. Load cartridge into rack Step 2. Transfer input samples Step 3. Load cartridges, samples, adapters and labware Step 4. Insert method card, select size range and press START
SPRIworks HT High Throughput System For Illumina next generation sequencers Sample Prep Freedom for High Throughput Next Generation Labs Built In SPRI and Cleanup High throughput library construction without running gels, cutting bands or using columns for cleanup. Patented SPRI chemistry is combined with the flexibility of the Beckman Coulter Biomek FX P liquid handler to revolutionize how next generation sequencing samples are prepared. All chemistry needed to prepare libraries is included in a kit format along with the reagents to perform size selection and chemistry cleanup. The only thing left to do is add the DNA sample and adapter of choice. Because SPRIworks HT is automated on the Biomek automated liquid handler, users have access to a suite of methods to further streamline the next generation sequencing workflow. Such methods include: setup, (on deck with T-robot integration), cleanup, quantitation, normalization, and sample pooling. Fully automated library construction process Less than 30 minutes of hands-on time High throughput Up to 96 high quality, fully constructed and sized libraries in under 6 hours Up to 25 times the throughput of manual library construction, similar to having 25 additional people to do next generation sequencing sample preparation Simple graphical user interface Includes SPRI size selection and cleanup 150-350 size selection Short fragment sizes isolated using SPRIworks HT High Throughput System and analyzed using the Agilent High Sensitivity DNA Chip. Illumina paired end adapters were used, samples were purified with Agencourt AMPure XP and amplified with 10 cycles of. High Throughput Library Construction With an Intuitive Easy to Use Interface, Everyone is an Expert Step 1. Configure parameters using the intuitive user interface Up to 96 high quality, fully constructed and sized libraries in under 6 hours. Throughput DNA input amount Size selection options Supported input sample types SPRIworks HT Up to 96 samples in under 6 hours 1 µg 150-350 bp (insert sizes) 250-450 bp (insert sizes) 350-700 bp (insert sizes) Sheared DNA, gdna, cdna, amplicons Step 2. Method calculates reagent volumes based on user input Step 3. Place tips, plates and reagents on the instrument, click OK
Ordering Information In the United States and Canada call +1 800 361 7780 In other countries, please contact your local representative or call the country number listed below. SPRIworks Fragment Library Systems (Up to 30 libraries/day) Product # SPRIworks System I (Includes instrument, cartridges, method card and labware) A88267 SPRIworks Fragment Library kit I (For Illumina Genome Analyzer) 10 cartridges A84801 SPRIworks Fragment Library kit I (For Illumina Genome Analyzer) 50 cartridges A84803 SPRIworks System II (Includes instrument, cartridges, method card and labware) SPRIworks Fragment Library kit II (For Roche GS FLX DNA Sequencer) 10 cartridges SPRIworks Fragment Library kit II (For Roche GS FLX DNA Sequencer) 50 cartridges SPRIworks System III (Includes instrument, cartridges, method card and labware) SPRIworks Fragment Library kit III (For Life Technologies SOLiD Sequencer) 10 cartridges SPRIworks Fragment Library kit III (For Life Technologies SOLiD Sequencer) 50 cartridges A88268 A84805 A84806 A98473 A98476 A98477 SPRIworks HT System (Up to 96 libraries/day) Product # SPRIworks HT Reagent kit (For Illumina next generation sequencers) 48 samples B06938 Biomek FX P Dual Arm Multi-96 and Span-8 Workstation - SPRIworks HT 1 B05450 2 SPRIworks HT Automation Consumables (Includes Biomek tips, plates, tubes, etc. to process 960 samples) B05451 1 Contact your automation sales specialist for specific configurations. +1 800 742 2345 2 The Biomek Workstation (Product # B05450) is intended for 110V operation only. For more information, please visit www.beckmancoulter.com or contact your local sales representative. For technical support in the United States and Canada call +1 800 854 3633 (Option #, 1, 3, 3). In other countries, please contact your local representative or call the country number listed below. The process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd. * All trademarks are property of their respective owners. Beckman Coulter, the stylized logo, AMPure, Biomek, SPRI and SPRIworks are registered trademarks of Beckman Coulter, Inc. For Research Use Only. Not for use in diagnostic procedures. Australia, Gladesville (61) 2 9844 6000 Austria, Vienna (43) 1 291 01 241 Canada, Mississauga (1) 905 819 1234 China, Shanghai (86) 21 3865 1000 Croatia, Zagreb (38) 51 489 9003 Czech Republic, Prague (420) 272 01 73 32 Eastern Europe, Middle East, North Africa, South West Asia: Switzerland, Nyon (41) 22 365 3707 France, Villepinte (33) 1 49 90 90 00 Germany, Krefeld (49) 2151 33 35 Hong Kong (852) 2814 7431 India, Mumbai (91) 22 3080 5000 Italy, Cassina de Pecchi, Milan (39) 02 953921 Japan, Tokyo (81) 3 5530 8500 Korea, Seoul (82) 2 404 2146 Latin America (1) (305) 380 4709 Malaysia, Kuala Lumpur (60) 3 5621 4793 Mexico, Mexico City (001) 52 55 9183 2800 Netherlands, Woerden (31) 348 462462 Poland, Warszawa (48) 22 366 0180 Puerto Rico (787) 747 3335 Singapore (65) 6339 3633 South Africa/Sub-Saharan Africa, Johannesburg (27)11 564 3203 Spain, Madrid (34) 91 3836080 Sweden, Bromma (46) 8 564 85 900 Switzerland, Nyon (41) 0800 850 810 Taiwan, Taipei (886) 2 2730 2500 Turkey, Istanbul (90) 216 570 17 17 UK, High Wycombe (44) 01494 441181 USA, Brea, CA (1) 800 352 3433, (1) 714 993 5321 B2011-12619 www.beckmancoulter.com 2011 Beckman Coulter, Inc. PRINTED IN U.S.A.