Fisher BioReagents Buffers for Life Science Research

Fisher BioReagents Buffers for Life Science Research

The Water Makes a Difference. CellPURE Biological Buffers are of the highest quality (purity 99%). They are tested for low heavy metal content and the absence of endotoxins, nucleases, and proteases. It is important to use only water of the highest quality to prepare the buffer solutions. Water that stands too long in pipes or water produced by a system needing routine maintenance increases the risk for contamination of buffer solutions. Fisher BioReagents provide several grades of high purity water suitable for the preparation of high quality buffer solutions. Molecular grade water Cat. No. Description Pack Size List price Promo Price FSBBP2485-4 Sterile biotech grade 4L $128.50 $102.80 FSBBP2485-20 Sterile biotech grade 20L $251.70 $201.40 FSBBP2484-50 Sterile DEPC treated nuclease free 500mL $72.10 $57.70 FSBBP2484-100 Sterile DEPC treated nuclease free 100mL $43.70 $35.00 FSBBP2470-1 DNA Grade 1L $69.50 $55.60 FSBBP561-1 RNA Grade, DEPC-treated 1L $123.90 $99.10 2

Reagents for Life Science Applications Packaged for convenience, our superior quality, value-added products are prequalified and guaranteed to be suitable for your designated technique or application. For higher productivity in your routine research techniques, choose Fisher BioReagents products Contents CellPURE Biological Buffers Application examples: Tissue culture media and maintenance of cell lines Enzyme assays Electrophoresis of RNA Transfection of mammalian cells JustPURE "Good" Buffers Application examples: High efficiency transfection of mammalian cells Gel electrophoresis of RNA Protein isolation applications Cell cultures and enzyme assays Bioanalytical methods such as IEF, 2-D electrophoresis, SDS-PAGE Additional Buffers from Fisher BioReagents Application examples: Electrophoresis of nucleic acids and proteins Isolation and purification of biomolecules Cell and tissue culture Guaranteed DNase-, RNase-, and protease-free 3

CellPURE Biological Buffers Fisher BioReagents buffers meet the needs of every budget and scale Economical powders come in various package sizes Concentrated stock solutions provide convenience in one step of easy dilution Ready-to-use solutions offer the biggest time savings of all Ultrapure zwitterionic biological buffers Optimised for cell/tissue culture work Wide applicability to molecular biology and biochemical studies Manufactured under strict quality control guidelines to ensure performance and reliabilty Obtaining optimal results in your research requires careful selection of reagents. When your experiments require exact buffering conditions, you can depend on the reliability of Fisher BioReagents buffers. All Fisher BioReagents buffers are manufactured from high-quality raw materials under ISO 9001:2008-certified manufacturing and testing processes. The purpose of a buffer in a biological system is to maintain intracellular and extracellular ph within the physiological range and resist changes in ph due to the presence of internal and external influences. CellPURE Biological Buffers from Fisher BioReagents are ultrapure zwitterionic buffers that possess both positive and negative charges. First described by Good and co-workers in 1966, these organic buffers have pka values at or near physiological ph and minimal interference with biological processes. CellPURE Biological Buffers are ideal for cell cultivation, isolation of cells, enzyme assays, and other biochemical applications. (Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): 467-477) Advantages Cell culture tested Analysed for the absence of nucleases and proteases Tested for endotoxin and bioburden levels pka values mostly independent of temperature and concentration High water solubility Minimal permeability to biological membranes Applications Tissue culture media and maintenance of cell lines Enzyme assays Electrophoresis of RNA Transfection of mammalian cells CellPURE Buffers Cat. No. Description Pack Size List Price Promo Price FSBBP2941-100 BIS-TRIS 100g $249.60 $199.70 FSBBP2943-100 BIS-TRIS propane 100g $254.10 $203.30 FSBBP2936-100 MOPS 100g $174.10 $139.30 FSBBP2946-25 MOPS sodium salt 25g $139.70 $111.80 FSBBP2937-100 HEPES 100g $214.80 $171.80 FSBBP2939-100 HEPES sodium salt 100g $232.70 $186.20 4

JustPURE Good Buffers Zwitterionic buffers possessing both positive and negative charges were described in 1966 by Good and co-workers as being suitable for work with biological molecules. Popularly known today as Good buffers, these organic buffers have several advantages compared to traditional inorganic buffering systems such as phosphate, borate, and bicarbonate. Good buffers approach the ideal buffer state by having pka values at or near physiological ph and minimal interference with biological processes. JustPURE Good buffers from Fisher BioReagents have very high purity (assay > 99%) and only trace amounts of metal ions, which makes them useful for applications requiring tight control of elemental content. (Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): 467-477) Advantages pka values mostly independent of temperature and concentration High water solubility Chemically and enzymatically stable Minimal permeability to biological membranes Limited effect on biochemical reactions Minimal absorption in spectral range 240 to 700nm Applications High efficiency transfection of mammalian cells Gel electrophoresis of RNA Protein isolation applications Cell cultures and enzyme assays Bioanalytical methods such as IEF, 2-D electrophoresis, SDS-PAGE JustPURE Buffers Cat. No. Description Pack Size List Price Promo Price FSBBP2920-250 MES hydrate 250g $1,038.30 $830.70 FSBBP2935-100 ACES 100g $507.10 $405.70 FSBBP2924-50 PIPES 50g $205.60 $164.50 FSBBP2929-25 BIS-TRIS propane 25g $476.50 $381.20 FSBBP2925-100 MOPS 100g $270.80 $216.60 FSBBP2921-50 HEPES 50g $426.70 $341.40 FSBBP2933-100 EPPS 100g $201.70 $161.40 FSBBP2922-100 Tricine 100g $258.80 $207.10 FSBBP2930-50 Gly-Gly 50g $276.70 $221.30 FSBBP2923-100 TAPS 100g $401.50 $321.20 FSBBP2934-100 CHES 100g $271.20 $217.00 FSBBP2928-100 CAPS 100g $221.43 $177.15 5

Buffer Properties Properties of CellPURE Biological Buffers Cat. No. Product Description Formula Molecular Weight pka @ 25º C Useful ph Range Cell Culture Tested Endotoxin Assay Nuclease and Protease Tested Size FSBBP2941-100 BIS-TRIS C 8 H 19 NO 5 209.24 6.5 5.8 to 7.2 X X X 100g FSBBP2943-100 BIS-TRIS propane C 11 H 26 N 2 O 6 282.33 6.8 6.3 to 9.5 X X X 100g FSBBP2936-100 MOPS C 7 H 15 NO 4 S 209.26 7.2 6.5 to 7.9 X X X 100g FSBBP2946-25 MOPS sodium salt C 7 H 14 NNaO 4 S 231.25 7.2 6.5 to 7.9 X X X 25g FSBBP2937-100 HEPES C 8 H 18 N 2 O 4 S 238.3 7.5 6.8 to 8.2 X X X 100g FSBBP2939-100 HEPES Sodium Salt C 8 H 17 N 2 NaO 4 S 260.29 7.5 6.8 to 8.2 X X X 100g pka = 9.0 for the second dissociation stage Properties of JustPURE Buffers Cat. No. Product Description CAS No. Formula Molecular Weight pka @ 25º C Useful ph Range Form Size FSBBP2920-250 MES hydrate 4432-31-9 C 6 H 13 NO 4 S xh 2 O 195.24 (anhydrous) 6.1 5.5 to 6.7 White powder 250g FSBBP2935-100 ACES 7365-82-4 C 4 H 10 N 2 O 4 S 182.2 6.8 6.1 to 7.5 White powder 100g FSBBP2924-50 PIPES 5625-37-6 C 8 H 18 N 2 O 6 S 2 302.37 6.8 6.1 to 7.5 White powder 50g FSBBP2929-25 BIS-TRIS propane 64431-96-5 C 11 H 26 N 2 O 6 282.33 6.8 6.3 to 9.5 White powder 25g FSBBP2925-100 MOPS 1132-61-2 C 7 H 15 NO 4 S 209.26 7.2 6.5 to 7.9 White powder 100g FSBBP2921-50 HEPES 7365-45-9 C 8 H 18 N 2 O 4 S 238.3 7.5 6.8 to 8.2 White powder 50g FSBBP2933-100 EPPS 16052-06-5 C 9 H 20 N 2 O 4 S 252.33 8 7.3 to 8.7 White powder 100g FSBBP2922-100 Tricine 1/04/5704 C 6 H 13 NO 5 179.17 8.1 7.4 to 8.8 White powder 100g FSBBP2930-50 Gly-Gly 556-50-3 C 4 H 8 N 2 O 3 132.12 8.2 7.5 to 8.9 White powder 50g FSBBP2923-100 TAPS 29915-38-6 C 7 H 17 NO 6 S 243.28 8.4 7.7 to 9.1 White powder 100g FSBBP2934-100 CHES 103-47-9 C 8 H 17 NO 3 S 207.29 9.3 8.6 to 10.0 White powder 100g FSBBP2928-100 CAPS 1135-40-6 C 9 H 19 NO 3 S 221.32 10.4 9.7 to 11.1 White powder 100g pka = 9.0 for the second dissociation stage 6

Additional Buffers from Fisher BioReagents Choose Fisher BioReagents Buffers for: QUALITY: tight specifications CONSISTENCY: lot-to-lot uniformity SELECTION: powders, concentrated stock solutions, or ready-to-use liquids PACKAGING: state-of-the-art containers designed for safety and utility CONVENIENCE: pre-qualified for a variety of applications ECONOMY: configurations to meet all budgets SCALE: from bench to batch sizes Suitable for Life Science applications: Electrophoresis of nucleic acids and proteins Isolation and purification of biomolecules Cell and tissue culture Guaranteed DNase-, RNase-, and protease-free Fisher BioReagents buffers meet the needs of every budget and scale. Economical powders come in various package sizes Concentrated stock solutions provide convenience in one step of easy dilution Ready-to-use solutions offer the biggest time savings of all Cat. No. Description Pack Size List price Promo Price FSBBP1306-1 10x Tris-Glycine 1L $108.90 $87.10 FSBBP1306-4 10x Tris-Glycine 4L $266.90 $213.60 FSBBP1307-1 10x Tris-Glycine 1L $134.10 $107.30 FSBBP1341-1 10x Tris-Glycine-SDS 1L $107.70 $86.20 FSBBP1341-4 10x Tris-Glycine-SDS 4L $218.60 $174.90 FSBBP1398-92 5x Tris-Glycine-SDS 1L $106.00 $84.80 FSBBP1342-1 10x Tris-Glycine-SDS 1L $88.20 $70.60 FSBBP2436-200 10% Sodium Dodecyl Sulphate 200mL $55.50 $44.40 FSBBP2436-1 10% Sodium Dodecyl Sulphate 1L $108.90 $87.20 FSBBP1311-200 20% Sodium Dodecyl Sulphate 200mL $71.40 $57.20 FSBBP1311-1 20% Sodium Dodecyl Sulphate 1L $127.30 $101.90 FSBBP166-100 Sodium Dodecyl Sulphate Powder 100g $54.80 $43.90 FSBBP166-500 Sodium Dodecyl Sulphate Powder 500g $160.00 $128.00 FSBBP166-5 Sodium Dodecyl Sulphate Powder 5kg $970.60 $776.50 7

Buffers for Nucleic Acid Electrophoresis Cat. No. Description Pack Size List price Promo Price FSBBP2430-1 1x Tris-Borate-EDTA 1L $82.90 $66.30 FSBBP2430-4 1x Tris-Borate-EDTA 4L $148.80 $119.10 FSBBP2430-20 1x Tris-Borate-EDTA 20L $402.30 $321.90 FSBBP1396-86 5x Tris-Borate-EDTA 1L $136.30 $109.10 FSBBP1333-1 10x Tris-Borate-EDTA 1L $88.90 $71.10 FSBBP1333-4 10x Tris-Borate-EDTA 4L $221.70 $177.40 FSBBP1333-20 10x Tris-Borate-EDTA 20L $1,011.60 $809.30 FSBBP1334-1 10x Tris-Borate-EDTA 1L $83.90 $67.10 FSBBP2434-4 1x Tris-Acetate-EDTA 4L $152.10 $121.70 FSBBP2434-20 1x Tris-Acetate-EDTA 20L $420.80 $336.60 FSBBP1335-500 10x Tris-Acetate-EDTA 500mL $58.80 $47.00 FSBBP1335-1 10x Tris-Acetate-EDTA 1L $73.40 $58.70 FSBBP1335-4 10x Tris-Acetate-EDTA 4L $195.90 $156.70 FSBBP1335-20 10x Tris-Acetate-EDTA 20L $681.30 $545.10 FSBBP1330-1 25x Tris-Acetate-EDTA 1L $208.70 $167.00 FSBBP1332-500 50x Tris-Acetate-EDTA 500mL $73.30 $58.70 FSBBP1332-1 50x Tris-Acetate-EDTA 1L $116.40 $93.10 FSBBP1332-4 50x Tris-Acetate-EDTA 4L $360.80 $288.60 FSBBP1332-20 50x Tris-Acetate-EDTA 20L $1,227.70 $982.20 FSBBP1331-1 25x Tris-Acetate-EDTA 1L $137.70 $110.20 FSBBP308-100 MOPS Buffer Powder 100g $60.30 $48.20 FSBBP308-500 MOPS Buffer Powder 500g $279.00 $223.20 FSBBP2900-500 10x MOPS Buffer Solution 500mL $115.60 $92.50 FSBBP2900-1 10x MOPS Buffer Solution 1L $104.30 $83.50 Buffers for Hybridization of Nucleic Acids Cat. No. Description Pack Size List price Promo Price FSBBP1328-1 20x Saline-Sodium Phosphate-EDTA 1L $84.40 $67.50 FSBBP1328-4 20x Saline-Sodium Phosphate-EDTA 4L $167.10 $133.70 FSBBP1328-20 20x Saline-Sodium Phosphate-EDTA 20L $434.30 $347.40 FSBBP1325-1 20x Saline-Sodium Citrate 1L $67.70 $54.20 FSBBP1325-4 20x Saline-Sodium Citrate 4L $171.00 $136.80 FSBBP1325-20 20x Saline-Sodium Citrate 20L $339.50 $271.60 8

Buffers for Life Science Applications Cat. No. Description Pack Size List price Promo Price FSBBP2482-100 0.5M EDTA, ph 8.0 100mL $46.90 $37.50 FSBBP2482-500 0.5M EDTA, ph 8.0 500mL $80.40 $64.30 FSBBP2482-1 0.5M EDTA, ph 8.0 1L $108.80 $87.10 FSBBP2482-20 0.5M EDTA, ph 8.0 20L $581.70 $465.40 FSBBP2483-100 0.5M EDTA, DEPC, ph 8.0 100mL $47.60 $38.10 FSBBP2483-500 0.5M EDTA, DEPC, ph 8.0 500mL $88.80 $71.00 FSBBP2483-1 0.5M EDTA, DEPC, ph 8.0 1L $118.80 $95.00 FSBBP118-500 EDTA Powder 500g $133.20 $106.60 FSBBP299-100 1.0M HEPES, ph 7.3 100mL $125.10 $100.10 FSBBP299-500 1.0M HEPES, ph 7.3 500mL $198.20 $158.60 FSBBP299-1 1.0M HEPES, ph 7.3 1L $341.80 $273.40 FSBBP310-100 HEPES Crystals 100g $58.30 $46.60 FSBBP310-500 HEPES Crystals 500g $175.60 $140.50 FSBBP310-1 HEPES Crystals 1kg $303.50 $242.80 FSBBP310-5 HEPES Crystals 5kg $1,297.50 $921.00 ACR172570000 HEPES for biochemistry 99% $2,240.40 $1,506.40 FSBBP2944-100 PBS Tablets 100 tablets $253.60 $202.90 FSBBP2438-4 1x PBS 4L $163.70 $130.90 FSBBP2438-20 1x PBS 20L $376.10 $300.90 FSBBP399-500 10x PBS 500mL $106.60 $85.30 FSBBP399-1 10x PBS 1L $173.40 $138.70 FSBBP399-4 10x PBS 4L $921.50 $737.20 FSBBP399-20 10x PBS 20L $134.60 $107.70 FSBBP661-10 1x PBS Powder Concentrate 10L $358.10 $286.50 FSBBP661-50 1x PBS Powder Concentrate 50L $72.80 $58.20 FSBBP2938-10 PBS Powder with Tween 20 10 pouches $229.60 $183.70 FSBBP665-1 10x PBS Powder Concentrate 2 x 1L $61.00 $48.80 FSBBP2478-1 1x Sodium Chloride-Tris-EDTA, ph 8.0 1L $82.90 $66.30 FSBBP2479-1 10x Sodium Chloride-Tris-EDTA, ph 8.0 1L $84.30 $67.40 FSBBP2472-1 1x Tris-Buffered Saline, ph 7.4 1L $98.20 $78.60 FSBBP2471-100 10x Tris-Buffered Saline, ph 7.4 100mL $45.50 $36.40 FSBBP2471-1 10x Tris-Buffered Saline, ph 7.4 1L $100.40 $80.30 9

Buffers for Life Science Applications Cat. No. Description Pack Size List price Promo Price FSBBP2476-100 1x Tris-EDTA, ph 7.4 100mL $44.80 $35.90 FSBBP2476-500 1x Tris-EDTA, ph 7.4 500mL $59.10 $47.30 FSBBP2476-1 1x Tris-EDTA, ph 7.4 1L $44.30 $35.50 FSBBP2473-100 1x Tris-EDTA, ph 8.0 100mL $63.20 $50.50 FSBBP2473-500 1x Tris-EDTA, ph 8.0 500mL $92.00 $73.60 FSBBP2473-1 1x Tris-EDTA, ph 8.0 1L $44.70 $35.80 FSBBP2474-100 1x Tris-EDTA, ph 7.6 100mL $63.20 $50.60 FSBBP2474-500 1x Tris-EDTA, ph 7.6 500mL $91.00 $72.80 FSBBP2474-1 1x Tris-EDTA, ph 7.6 1L $95.40 $76.30 FSBBP2477-1 10x Tris-EDTA, ph 7.4 1L $44.90 $35.90 FSBBP2475-100 10x Tris-EDTA, ph 7.6 100mL $64.90 $51.90 FSBBP2475-500 10x Tris-EDTA, ph 7.6 500mL $99.30 $79.50 FSBBP2475-1 10x Tris-EDTA, ph 7.6 1L $111.10 $88.90 FSBBP1338-1 100x Tris-EDTA, ph 8.0 1L $193.10 $154.50 FSBBP1339-1 100x Tris-EDTA 1L $65.70 $52.60 FSBBP1761-500 0.3M Tris Buffer 500mL $100.40 $80.30 FSBBP1761-1 0.3M Tris Buffer 1L $46.70 $37.30 FSBBP1759-100 2.0M Tris Buffer 100mL $78.10 $62.50 FSBBP1759-500 2.0M Tris Buffer 500mL $55.70 $44.60 FSBBP1756-100 1.0M Tris Hydrochloride, ph 7.0 100mL $130.40 $104.40 FSBBP1756-500 1.0M Tris Hydrochloride, ph 7.0 500mL $165.30 $132.20 FSBBP1757-100 1.0M Tris Hydrochloride, ph 7.5 100mL $117.50 $94.00 FSBBP1757-500 1.0M Tris Hydrochloride, ph 7.5 500mL $54.20 $43.40 FSBBP1758-100 1.0M Tris Hydrochloride, ph 8.0 100mL $104.50 $83.60 FSBBP1758-500 1.0M Tris Hydrochloride, ph 8.0 500mL $228.60 $182.90 FSBBP153-500 Tris Hydrochloride Solid 500g $382.40 $305.90 FSBBP153-1 Tris Hydrochloride Solid 1kg $111.10 $88.90 FSBBP152-500 Tris Base 500g $209.50 $167.60 FSBBP152-1 Tris Base 1kg $514.90 $411.90 FSBBP152-5 Tris Base 5kg $1,213.60 $970.90 FSBBP152-10 Tris Base 10kg $2,520.10 $2,016.10 FSBBP152-25 Tris Base 25kg $2,783.70 $2,226.95 10

EZ-RUNyou Protein Standards Have tried our EZ-Run Protein Standards? Designed to assist in identifying and characterizing unknown proteins inhighly purified markers ladders provide compact and clear bands polyacrylamide gels andand immunoblots -Prestained standards areand indispensable in monitoring protein separation Highly purified markers ladders provide compact and clear bands -and Prestained transfer standards efficiencyare indispensable in monitoring protein separation and transfer -Reference allow quick quick gel gelprogress progressassessment assessment Referencebands band allows Unstainedstandards standards are are most of of proteins -Unstained most suitable suitablefor forprecise precisesizing sizing proteins - All standards are supplied in loading buffer and are ready to use All standards are supplied in loading buffer and are ready to use kda kda 12% SDS-PAGE 12% SDS-PAGE EZ-RUN Prestained Protein Marker BP3601-500 and BP3601-1 EZ-RUN Protein Marker BP3600-500 and BP3600-1 ORDERING INFORMATION MW Range No. of Bands Description MW Range No. of Bands Unstained Protein Standards 14.4-116.0 kda ---- 10.0-200.0kDa 14 7 50kDa 11.0-170.0kDa 10 11.0-170.0 kda 50 kda ---72kDa 6 10 kda 4-20% SDS-PAGE 8-16% SDS-PAGE EZ-RUN Rec Protein Ladder BP3602-500 and BP3602-1 EZ-RUN Prestained Rec Protein Ladder BP3603-500 and BP3603-1 Reference Band Reference Band Source 14.4-116.0kDa 7 Prestained Protein 10.0-200.0 kda 14 Standards 20.0-118.0kDa 6 20.0-118.0 kda kda Source Quantity Unstained protein standards 500µL Native proteins 2 x 500µL Recombinant proteins 500µL Native proteins 2 x 500µL BP3600-500 BP3600-1 BP3602-500 500 μl BP3602-1 Recombinant proteins Native proteins 500µL 2 x 500µL Recombinant proteins 500µL 2 x 500µL Prestained Protein Standards 500 μl BP3601-500 BP3601-1 2 x 500 μl BP3603-500 BP3603-1 Native proteins 72 kda Quantity Catalog No. Recombinant proteins Catalog No. FSBBP3600-500 FSBBP3602-500 FSBBP3602-1 500 μl FSBBP3601-500 2 x 500 μl FSBBP3601-1 500 μl FSBBP3603-500 2 x 500 μl FSBBP3603-1 11

Cut and Keep Preparation of Polyacrylamide Stacking and Separating Gels Separating Gel (total volume, 15 ml) Final Percentage Acrylamide in Gel Stock solutions (total volume 15 ml) Component 5% 6% 7% 7.50% 8% 9% 10% 12% 13% 15% 30% Acrylamide/0.8%Bis- Acrylamide 2.50 ml 3.00 ml 3.50 ml 3.75 ml 4.00 ml 4.50 ml 5.00 ml 6.00 ml 6.50 ml 7.50 ml 4X Tris Cl, ph 8.8 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml H 2 O 8.60 ml 8.10 ml 7.60 ml 7.35 ml 7.10 ml 6.60 ml 6.10 ml 5.10 ml 4.60 ml 3.60 ml 10% SDS 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 10% Ammonium Persulfate 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl TEMED 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 4% Stacking Gel (total volume, 5mL) Component Volume 30% Acrylamide/0.8%Bis-Acrylamide 0.65 ml 4X Tris Cl, ph 6.8 1.25 ml H 2 O 3.00 ml 10% SDS 50 μl 10% Ammonium Persulfate 25 μl TEMED 5 μl Procedure for Gel Preparation 1. In a 25 ml sidearm flask or tube, mix the given volumes of: - Acrylamide/Bis-Acrylamide solution. - Tris-HCl Buffer - H 2 O 2. Degas under vacuum 10-15 minutes 3. Add DS solution, Ammonium Persulfate solution and TEMED 4. Swirl gently to mix 5. Use immediately Notes: Volumes incuded here are adequate for a gel of dimensions 0.75 cm x 14 cm x 14 cm Recipes are based on the SDS (denaturing)-continuous buffer system of Laemmli (1970) the % acrylamide selected for the separating gel will depend on the moleular sizes of the proteins being separated. All reagents and solutions used in this protocol must be prepared with distilled deionised water. Store at 4ºC for a maximum 5 days Cut here to save this protocol for later Order Placement: To fax an order, use 0800-329-246 To phone, call 0800-933-966 To email an order, ordersnz@thermofisher.com To order online: thermofisher.co.nz Contact Us: For customer service, call 0800-933-966 For service and calibration, call 0800-933-966 By email, chemicalsnz@thermofisher.com Find out more at thermofisher.co.nz *Pricing valid until 30th September 2017. All Prices are in NZ dollars and exclude GST. All offers subject to Thermo Fisher standard terms and conditions. Errors and omission exempted. Specifications, terms and pricing are subject to change. Please consult your local sales representative for details. 2017 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. 1491866521