DEVELOPMENT OF AN AUTONOMOUS UNMANNED AERIAL VEHICLE FOR AEROBIOLOGICAL SAMPLING. A Thesis

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DEVELOPMENT OF AN AUTONOMOUS UNMANNED AERIAL VEHICLE FOR AEROBIOLOGICAL SAMPLING A Thesis Presented to the Faculty of Virginia Polytechnic Institute and State University In Partial Fulfillment of the Requirements for the Degree of Master of Science in Mechanical Engineering Dr. Charles F. Reinholtz, Committee Chair Department of Mechanical Engineering Dr. David G. Schmale III, Committee Co-Chair Department of Plant Pathology, Physiology, & Weed Science Dr. Alfred L. Wicks Department of Mechanical Engineering May 8, 2007 Blacksburg, Virginia 2007 Benjamin Ross Dingus

DEVELOPMENT OF AN AUTONOMOUS UNMANNED AERIAL VEHICLE FOR AEROBIOLOGICAL SAMPLING Benjamin Ross Dingus, M.S. ABSTRACT The ability to detect, monitor, and forecast the movement of airborne plant pathogens in agricultural ecosystems is essential for developing rational approaches to managing these habitats. We developed an autonomous (self-controlling) unmanned aerial vehicle (UAV) platform for aerobiological sampling tens to hundreds of meters above agricultural fields. Autonomous UAVs have the potential to extend the range of aerobiological sampling, improve positional accuracy of sampling paths, and enable coordinated flight with multiple aircraft at different altitudes. We equipped a Senior Telemaster model airplane with two spore-sampling devices and a MicroPilot autonomous system, and we conducted over 60 autonomous microbe-sampling flights at Virginia Tech s Kentland Farm. To determine the most appropriate sampling path for aerobiological sampling, we explored a variety of different sampling patterns for our autonomous UAVs including multiple GPS waypoints plotted over a variety of spatial scales. We conducted a total of 25 autonomous aerobiological sampling flights for five different aerobiological sampling patterns. The pattern of a single waypoint exhibited the best flight characteristics with good positional accuracy and standard deviations in altitude from 1.6 to 2.8 meters. The four point pattern configured as a rectangle also demonstrated good flight characteristics and altitude standard deviations from 1.6 to 4.7 meters.

BIOGRAPHICAL SKETCH Ben was born and raised in Nickelsville, Virginia, and attended Southwest Virginia Community College for the first two years of undergraduate. After which he transferred to Virginia Tech to finish his undergraduate degree. At Virginia Tech, he has worked on many unmanned systems projects including the student UAV team to compete in AUVSI s annual competition (2005-2006) and doing undergraduate research with Dr. Reinholtz on the ONR vehicle teaming project (2006). During graduate school, Ben has worked with the Department of Plant Pathology for the work presented in this thesis with a defense date of May 8, 2007. He also works for Dr. Reinholtz as a graduate research assistant on the ONR vehicle teaming experiments and is a teaching assistant for the student UAV competition team (2006-2007). When he is not doing research, Ben enjoys mountain biking, hiking, and hanging out with friends. iii

ACKNOWLEDGEMENTS I would like to thank the members of my committee, Charles Reinholtz, David Schmale III, and Alfred Wicks. I would also like to extend my gratitude Dr. Reinholtz for persuading me to attend graduate school and Dr. Schmale for allowing me to work with him on this project and for taking time to participate in a large number of the flights that were conducted this year. I owe a large thanks to everyone involved in establishing Virginia Tech s Kentland Farm as a well maintained airstrip for without that, we could not fly. I would also like to thank my family for their love and support throughout my academic career because without them I would not be who I am today. iv

TABLE OF CONTENTS CHAPTER 1: BACKGROUND AND LITERATURE REVIEW OF THE USE OF UNMANNED AERIAL VEHICLES FOR AEROBIOLOGICAL SAMPLING Introduction...1 Study of the potato leafhopper in the lower atmosphere...2 Study of the abundance of Gibberella zeae in the planetary boundary layer...3 Study of horseweed in the planetary boundary layer...4 Study of the use of autonomous systems in agriculture...4 CHAPTER 2: DESIGN OF AN AUTONOMOUS UNMANNED AERIAL VEHICLE FOR AEROBIOLOGICAL SAMPLING Introduction...7 Platform for an autonomous UAV...7 Autonomous system Flight controller...11 Control methods...13 Ground control station...13 Electronics box...14 Spore-samplers...16 CHAPTER 3: APPLICATION OF AN AUTONOMOUS UNMANNED AERIAL VEHICLE SYSTEM FOR CONSISTENT AEROBIOLOGICAL SAMPLING Introduction...22 Materials and methods Optimization of autonomous flight...23 v

Data collection during flight...23 Autonomous patterns for aerobiological sampling...24 Comparison to remote-controlled flight...30 Results/discussion...30 CHAPTER 4: EXTENDED WORK WITH NEW AND ALTERNATIVE AUTONOMOUS UNMANNED AERIAL VEHICLE SYSTEMS The Sig Rascal 110 as a new aerobiological sampling platform Platform...36 Electronics box...38 Redundant control via the RxMUX...39 Spore-samplers...41 CHAPTER 5: CONTRIBUTIONS AND RECOMMENDATIONS Contributions...44 Recommendations Coordinated sampling with the Piccolo autonomous system...44 Future work Night-time flight...45 Simultaneous UAVs for sampling at multiple altitudes...45 LITERATURE CITED...48 vi

LIST OF FIGURES Figure 2.1 Senior Telemaster ARF with oversized 1.20AX engine...8 Figure 2.2 Figure 2.3 Figure 2.4 Figure 2.5 The servo tray was relocated to the back of the fuselage of the Senior Telemaster ARF...9 The throttle servo was relocated near the firewall on the Senior Telemaster ARF...9 The battery tray was installed above servo tray for accessibility...10 Wing strut attachment method on the Senior Telemaster ARF...11 Figure 2.6 Screen shot of MicroPilot Horizon GCS software...14 Figure 2.7 Electronics project box with components installed...16 Figure 2.8 Design of previous spore-samplers (Maldonado-Ramirez et al., 2005) with sampling devices open (A) and sampling devices closed (B)...17 Figure 2.9 Figure 2.10 Figure 2.11 Figure 3.1 Figure 3.2 Figure 3.3 New and improved design of spore-samplers on the autonomous Senior Telemaster ARF...18 Plates of Fusarium-selective medium showing white Fusarium colonies collected 100 m above the surface of the earth at Virginia Tech s Kentland Farm...19 Culturable fraction of an atmospheric microbial community collected 100m above the surface of the earth at Virginia Tech s Kentland Farm. The plates contained a potato dextrose agar, a common medium for the cultivation of microbes...20 a single GPS waypoint. Individual flight patterns (A-E), combined flight patterns (F)...25 two GPS waypoints. Individual flight patterns (A-E), combined flight patterns (F)...26 three GPS waypoints. Individual flight patterns (A-E), combined flight patterns (F)...27 vii

Figure 3.4 Figure 3.5 Figure 3.6 four GPS waypoints. Individual flight patterns (A-E), combined flight patterns (F)...28 four GPS waypoints in a figure-eight pattern. Individual flight patterns (A-E), combined flight patterns (F)...29 Inconsistent flight pattern from a remote-controlled aerobiological sampling flight around a single GPS waypoint...30 Figure 3.7 Maintenance of a precise altitude during aerobiological sampling...33 Figure 4.1 Figure 4.2 Rascal 110 as a shown with samplers open (a) and samplers closed (b)...36 Relocated throttle servo to create space for autonomous unit and ensure consistent throttle response...38 Figure 4.3 Electronics box for Rascal 110...39 Figure 4.4 Figure 4.5 The RxMUX allows for redundant RC control from the ground for safety...41 Redesigned sampling devices allow for greater flexibility of media used...42 viii

LIST OF TABLES Table 3.1 Flight data from autonomous sampling flights...31 ix