Website: thermofisher.com Customer Service (US): 1 800 955 6288 ext. 1 Technical Support (US): 1 800 955 6288 ext. 441 mcherry Polyclonal Antibody Catalog Number PA5-34974 Product data sheet Details Size 100 µl Host/Isotope Class Type Immunogen Conjugate Form Concentration Purification Storage buffer Contains Storage Conditions Rabbit / IgG Polyclonal Antibody Recombinant fragment contains a sequence corresponding to a region within amino acids 1 and 236 of mcherry Unconjugated Liquid 0.36 mg/ml Antigen affinity chromatography PBS, ph 7, with 30% glycerol, 0.5% BSA 0.01% thimerosal -20 C, Avoid Freeze/Thaw Cycles Species Reactivity Tested species reactivity Tag Published species reactivity Not Applicable Tested Applications Dilution * Immunocytochemistry (ICC) 1:100-1:1000 Immunofluorescence (IF) 1:100-1:1000 Immunoprecipitation (IP) 1:3000 Western Blot (WB) 1:1000-1:10,000 Published Applications Miscellaneous PubMed (MISC) See 1 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Product specific information PA5-34974 targets mcherry in ICC, IF, IP, and WB applications and shows reactivity with Human samples. The PA5-34974 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 1 and 236 of mcherry.
Product Images For mcherry Polyclonal Antibody (PA5-34974) in IF Immunofluorescent analysis of mcherry in HeLa cells. mcherry fusion protientransfected HeLa cells were fixed in Triton X- 100 for 1 min, then 4% paraformaldehyde at RT for 30 min and stained using a mcherry polyclonal antibody (Product # PA5-34974) diluted at 1:500. Red: mcherry is expressed in the tranfected cell. Blue: Hoechst 33342 staining. (PA5-34974) in IF Immunofluorescent analysis of mcherry in 2 days-postfertilization zebrafish embryos. Embryos were stained using a mcherry polyclonal antibody (Product # PA5-34974) diluted at 1:100. (PA5-34974) in WB Western blot analysis of mcherry in Nontransfected (-) and PLK4 -transfected (+, inculding mcherry - tag) 293T whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with mcherry Polyclonal antibody (Product # PA5-34974) at a dilution of 1:5000.
(PA5-34974) in WB Western blot analysis of mcherry using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of mcherry-tagged protein expressing 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a mcherry polyclonal antibody (Product # PA5-34974) at a dilution of 1:5000.
(PA5-34974) in WB Western blot analysis of mcherry using A) 5ng recombinant mcherry (B) 10ng recombinant mcherry and C) 20ng recombinant mcherry. Samples were loaded onto a 12% SDS- PAGE gel and probed with a mcherry polyclonal antibody (Product # PA5-34974) at a dilution of 1:2000. (PA5-34974) in IP PA5-34974 immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A: 20 µg whole cell lysate /extract of mcherry protein expressing 293T cells B: Control with 2.5 µg of preimmune rabbit IgG C: Immunoprecipitation of mcherry protein by 2.5 µg of mcherry antibody (Product # PA5-34974) on 7.5% SDS-PAGE.
PubMed References For mcherry Polyclonal Antibody 1 Miscellaneous PubMed References Species / Dilution Summary PA5-34974 was used in immunocytochemistry and western blot to describe a missense mutation that affects both functions of gephyrin Not Applicable / Not Cited EMBO molecular medicine ( 2015; 7: 1580) "Simultaneous impairment of neuronal and metabolic function of mutated gephyrin in a patient with epileptic encephalopathy." Author(s):Dejanovic B,Djémié T,Grünewald N,Suls A,Kress V,Hetsch F,Craiu D,Zemel M,Gormley P,Lal D,Myers CT,Mefford HC,Palotie A,Helbig I,Meier JC,De Jonghe P,Weckhuysen S,Schwarz G PubMed Article URL:http://dx.doi.org/10.15252/emmm.201505323