Reaction Monitoring: InsightMR Robert Krull Senior Applications Scientist Pittsburgh April 2016 Innovation with Integrity
Outline Introduction InsightMR Flow Tube InsightMR Software Setup Data Analysis with Dynamics Center
Reaction Monitoring What is it and Why are we Doing it? Reaction completion determination In-situ yield determination Reaction understanding: mechanistic information Extracting kinetic parameters Stability, degradation studies Dissolution Bio transformations Process control - PAT FDA providing guidelines for PAT http://www.fda.gov/downloads/drugs/guidancecomplianceregulatoryinformation/guidances/ucm070305.pdf
Techniques for Reaction Monitoring Calorimetry LC-UV MS, GC, GC-MS IR In-line (in-situ) measurements possible! NMR» At-line» In-line» On-line Quantitative by default: no need to calculate response factors! Information rich: possible to elucidate the structure of starting materials, products, and intermediates.
* Patent Pending What is InsightMR? Hardware Software InsightMR flow tube* InsightMR software package
InsightMR Flow Tube April 14, 2016
Concept InsightMR flow tube is an insert for virtually any Bruker 5 mm NMR probe. Enables online monitoring of chemical reactions in real-time under real conditions Fast and continuous transfer of reactions mixtures from a reaction vessel into the flow tube and back Continuously circulates the liquid from a reaction vessel through the NMR probe. The changing composition of the liquid in the vessel, i.e. the progress of the reaction, can be continuously monitored. Temperature control from the vessel to the probe and back achieved by running sample lines through temperature controlled heat transfer fluid
Basic Schematic InsightMR Package Customer Supplied NMR Magnet
Flow Tube Assembly Overview
Easy to Replace Flowcell Tube is easy to remove Cell protector doubles as an installation tool
Laboratory Setup Coventry, UK
Enabling parallel acquisition of data from different spectroscopies
Flow Behavior Delay of Transfer (a) Integral corresponds to 15 mm composition (b) @27.75 min Composition in pump was changed to 100% (c) After 1 min @ 28.75 min change visible in NMR (d) After 1.75 min @ 29.5 min 90% of target composition reached (e) After ~3 min @ 30.5 min ~100% target composition reached (b) (e) (d) <1-2 min is possible by adjusting the flow rate to 2-3ml/min Adjustment of hardware (length diameter) also possible. (4 m length, 1 ml/min flow, 0.5 mm i.d. capillaries) (a) (c) Pump contributes ~100 ul to delay of concentration step propagation.
Line Shape Test 10 mm sucrose Stopped flow, 16% Flow 1 ml/min, 17% Flow 1 ml/min, after re-shimming, 15%
Fun Facts Available in 2 lengths: 4m and 7m Cell is 5mm tube Volume ~600ul Filling height ~40mm Sample Capillary Material: PTFE i.d.: 0.5mm Capillary volume (in & out): 4m ~1.6ml, 7m ~2.7ml Temperature Range: -5C to 60C Maximum Pressure (sample): 10bar Maximum Flow Rate (sample): 5ml/min.
InsightMR Software Integrates acquisition and analysis Gives the ability to assess and adjust acquisition parameters in real time April 14, 2016
Integration of Acquisition and Analysis Click Click Acquisition Spectra Display
Getting Started with InsightMR Obtain and install a copy of the InsightMR software* Available on the Topspin 3.5 DVD. Version 3.5pl5 recommended. Can be downloaded at www.bruker.com Registration required Search for insightmr Most up-to-date version Necessary components Can install in parallel Import configuration Prosol Cortab User specific files etc. *Windows available now Linux version in beta testing
Getting Started with InsightMR Obtain a Reaction Monitoring license NOTE: Valid Topspin 3.x license also required
InsightMR Software and License License request form at www.bruker.com
InsightMR Software and License Using search term insightmr produces hits for software download and license request as top 2 hits.
InsightMR Demo License NOTE: Not instantaneous, typically 1-2 business days
License File No REACTION_MONITORING_1 Feature
Getting Started with InsightMR After software has been installed and licensed Open Topspin CF expinstall Optional: Acquire a spectrum Close Topspin Open InsightMR Set default automation mode Verify license OK Check solvents and parameter sets
Getting Started with InsightMR Method Center active Solvents and Parameter sets OK
Getting Started with InsightMR After software has been installed and licensed Open Topspin CF expinstall Optional: Acquire a spectrum Close Topspin Open InsightMR Set default automation mode Verify license OK Check solvents and parameter sets Let s get started!
Getting Started with InsightMR LIVE DEMO
Getting Started with InsightMR Utilizes ICONNMR EZ-setup interface Choose sample position Choose or enter experiment name Choose solvent
Getting Started with InsightMR Utilizes ICONNMR EZ-setup interface Choose experiment parameters Optional: Add information to title
Getting Started with InsightMR Utilizes ICONNMR EZ-setup interface Adjust acquisition parameters Set number experiments (data points) Time between experiments (data points) Set reaction t=0 Adds experiments to the queue
Getting Started with InsightMR Experiments have been added to the queue
Getting Started with InsightMR Click on Start Choose mode Click on Start to initiate the run
Experiment Delay Change Delay between points modified during run
Modifying Acquisition Settings Find the IconNMR/InsightMR window (may be in background) Click on the Configuration button
IconNMR Configuration Settings holder InsightMR uses User ReactionMonitoring
Experiments Straightforward set up K_PROTON (zg30) K_WATER (zgcppr) K_NOESY1D (ns=8, NO presat) K_WET & K_WETDC (no deuteration, no lock) User selects number of peaks to suppress Program does the rest: Shim on 1 H, optimized for variety of solvents Scout scan to find solvent peaks Calculate shaped pulse for suppression Run suppressed spectrum Automatic referencing
IconNMR Configuration Settings Parameter sets available in InsightMR Easy to add your own parameter sets
Adding Experiments and Parameters
Solvents When importing a configuration it may be necessary to Restore default solvents if the new H solvents are not visible Don t forget to enable Show no locking solvents
Adding New Solvents Step 1 - Topspin 2 3 1 rename. E.g. test & add description
Adding New Solvents Step 2 - IconNMR 4
Lock and Shim
Adding New Solvents Step 2 - IconNMR 4 5
Protonated solvents Shimming: lctshim Special treatment for unlocked acquisition Set solvent dependent search range for Shim signal Activate RGA and compensation for flowing liquid (convcomp) Selective 1H shimming All automatically done with commandline parameter flow xau lctshim flow
Diels Alder Chemistry, NON-Deuterated Solvent CH3CN 25C DMF Maleic Anh. Product acetonitrile
Diels Alder Chemistry, NON-Deuterated Solvent 7 8 7 8
IconNMR Configuration Settings InsightMR uses Easy Setup Mode Separate IconNMR configuration files may be needed if normally running in the standard mode
IconNMR Configuration Settings Separate IconNMR configuration files can easily be saved and recalled for different automation modes.
Key Benefits A single interface for automated acquisition control, and interactive processing and analysis On-the-fly acquisition changes as needed based on real-time kinetic data Straightforward experiment set up. Default experimental parameters provided for both deuterated and non-deuterated solvents Rapid and effortless handling of hundreds of stacked spectra Supports acquisition, processing and analysis of series of 1Ds (different nuclei), interleaved experiments and multiple samples in parallel
InsightMR Team Project Manager Anna Codina Software Peter Neidig Mark Garvey Flow Tube/Hardware Martin Hofmann Ulrich Braumann Applications/Development Peter Gierth Eberhard Schweizer Nicola Tonge Aitor Moreno Rob Krull
www.bruker.com InsightMR@bruker.com Copyright Bruker Corporation. All rights reserved.