Management of Root-Knot Nematodes With Novel Nematicides

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Management of Root-Knot Nematodes With Novel Nematicides J. Ole Becker, Department of Nematology, UC Riverside, Antoon Ploeg, Department of Nematology, UC Riverside, Joe Nunez, UCCE Bakersfield, CA

SCREC Sandy loam infested with the Southern root-knot nematode, Meloidogyne incognita (43J2/100 cc); RCB, 5 replications Surface drip application Vigorous growth 5 wks after planting

Summer 2011 tomato trial at UC South Coast R&E Center nematicide application before incorporation

Shafter Research Station South Coast REC

20011 Treatments Trmt# Product 5 days before transplant (incorporated) 14 days after transplant (sprinkler can) 21 days after transplant (sprinkler can) 28 days after transplant (foliar) 1 Non-treated check 2 Vydate L 3 pt/a 4 pt/a 3 RDL-29 2.0 lb ai/a 1.0 lb ai/a 4 MCW-2 3.56 lbs a.i./a 5 NemaQ 3 gal/a 3 gal/a 6 Sesame 1 gal/a 1 gal/a 7 Actinovate 12 oz/a 8 Vydate + Movento 3 pt/a 2.5 oz/a 9 Ecozin Plus 56 fl oz/a 56 fl oz/a 10 Nortica 75 lbs/a

Root Galling 6 weeks after Transplanting Gall Rating 3 2.5 2 1.5 1 0.5 de b bcd a b e cde bc cde de 0 Control Vydate L RDL-29 MCW-2 NemaQ Sesamine Actinovate Vydate + Movento Ecozin Nortica Mean separation at LSD 0.05

Root gall Rating at Harvest 8 7 6 d b cd bc bcd bcd bc d cd Gall Rating 5 4 3 a 2 1 0 Control Vydate L RDL-29 MCW-2 NemaQ Sesamine Actinovate Vydate + Movento Ecozin Nortica Mean separation at LSD 0.05

Tomato Fruit Yield (Kg/plant) 1.8 1.6 1.4 1.2 Kg/plant 1 0.8 0.6 0.4 0.2 0 Control Vydate L RDL-29 MCW-2 NemaQ Sesamine Actinovate Vydate + Movento Ecozin Nortica Yields are not significant from each other

Summer 2011 tomato trial at UC South Coast R&E Center representative root systems (left to right): nt check, Vydate, MCW-2

2012 Trials South Coast Research & Extension Center & UCCE Shafter Research Farm

SCREC 2012

SCREC 2012

Shafter Processing Tomato Nematode Trial 2012 Mid-Season Root End of Sesaon Gall Rating* Root Gall Rating (7/20/12) (8/21/12) 1. Control 7.06 ABCD 10.00 A 2. Vydate 3 pt/a 3.48 F 9.56 A 3. Movento 4 fl oz/a x 2 app 7.86 AB 9.88 A 4. Sesamin EC 1 gal/a 8.38 A 10.00 A 5. Nema Q 3 gal/a 8.34 A 10.00 A 6. MeloCon 4 lb/a 8.28 A 9.68 A 7. Dazitol 6.25 gal/a 8.42 A 10.00 A 8. MCW-2 rate 1 6.25 liters/ha 5.40 CDEF 9.76 A 9. MCW-2 rate 2 8.33 liters/ha 6.06 BCDE 8.92 A 10. Dev Prod-1 1 pt/a 7.46 ABC 9.80 A 11. Dev Prod-1 1 qt/a 7.40 ABC 9.72 A 12. Dev Prod-1 1 pt & 0.5 pt post 5.16 DEF 8.96 A 13. Dev Prod-1 1 pt & 2 X 0.5 pt post 4.00 EF 7.24 B 14. Ecozin 56 fl oz/a 8.94 A 10.00 A Prob.= 0.0000 0.0032 % CV= 23.95 10.35 LSD p=0.05 = 2.090 1.253 *Root Gall Rating: 1-10, where 1 equals no gall present and 10 is 100% of root system galled.

Shafter Research Farm 2012 DP1 DP2 DP3 DP4 DP1 DP2 DP3 DP4

CONTROL

VYDATE

MCW-2 Low Rate

MCW-2 High rate

DP 1 pre & 1 post application

DP 1 pre & 2 post applications

2013 Trials South Coast Research & Extension Center & UCCE Shafter Research Farm

2013 SCREC Results Mid-season End-season Treatment Gall Rating Gall rating 1. Non-Treated Check 3.4 A 7.6 A 2. Vydate L 2.9 AB 6.2 BC 3. MCW-2 low 1.8 BC 6.5 AB 4. MCW-2 medium 1.2 C 4.9 CD 5. MCW-2 high 0.8 CD 4.5 DE 6. DP preplant 1.3 C 3.3 DEF 7. DP pre+post low 1.8 C 3.9 DEF 8. DP pre+post high 0.4 D 2.8 F 9. Nemaroot low 3.8 A 7.1 AB 10. Nemaroot high 4.0 A 7.6 A

Table 4. Average vigor of tomato 4 weeks after planting and harvest fruit yield per plant (kg) at SCREC during the 2013-growing season (n=5). Treatment z Vigor Yield per plant % diff. from non-treated check. 1. Non-treated check 6.4 abc y 1.18 cd 0 2. Vydate L 6.2 c 1.29 bcd +9 3. MCW-2 low 7.2 abc 1.32 bc +12 4. MCW-2 medium 7.8 a 1.49 ab +26 5. MCW-2 high 7.4 abc 1.37 abc +16 6. DP pre_only 7.4 abc 1.47 ab +24 7. DP pre+post_low 7.6 ab 1.41 abc +19 8. DP pre+post_high 7.6 abc 1.61 a +36 9. Nemaroot low 7.4 a 1.36 abc +15 10. Nemaroot high 6.2 bc 1.05 d -11

Nt check Vydate MCW-2 Dp

Nt check Vydate MCW-2 Dp

Tab. 5 Nematicide evaluation 2013 at Shafter Station (*root gall rating (1-5)) Average Nematode Rating* 1. Control 4.5 A 2. Vydate 3 pt/a 1 pre & 2 post app 3.0 D 3. Vydate 2 post app 3.5 CD 4. Movento 5 fl oz/a 4.4 AB 5. MCW-2 rate 1 668 ml/a pre 3.8 ABCD 6. MCW-2 rate 2 881 ml/a pre 3.3 CD 7. MCW-2 rate 1 668 ml/a pre & 1 post app 3.6 BCD 8. MCW-2 rate 1 668 ml/a pre & 2 post app 3.7 ABCD 9. MeloCon 6 lbs/a 4.1 ABC 10. Dp 1 pt/a pre 3.8 ABCD 11. Dp 1 pt pre & 1 post app @ ½ pt /A 3.5 CD 12. Dp 1 pt pre & 2 post app @ ½ pt /A 3.1 D Prob. 0.1107 %CV 24.48 LSD 0. 10 0.8744

Control

Vydate-pre & 2 post applications

DP-pre & 2 post applications

MCW-2-pre & 2 post applications

SCREC early root gall rating 7 weeks after transplanting Non-treated check vs MCW-2 (#5) Non-treated check vs Dp split (#8)

Non-treated check Vydate MCW-2 (#4) Dp split (#8) representative roots at harvest

Conclusions DP and Mana MCW-2 continue to show great promise as developing nematicide options

Conclusions MANA s MCW-2 is an excellent non-fumigant nematicide with great potential. Will be released in 2014 as Nimitz Supply will be limited in 2014 Label will carry a caution as signal word Development Product nematicide also has great potential No word when it could be available Vydate works best when applied pre-plant followed by post-plant applications

Conclusions Biologicals Performance is below chemicals and often times it s hit or miss with them More work is needed to find how to best use them Other chemistries There is renewed interest in developing non-fumigant nematicides. Other companies are in development stages of non-fumigant products.