Dicyclopentyl Dithiosquarate as an Intermediate for the Synthesis of Thiosquaramides

Dicyclopentyl Dithiosquarate as an Intermediate for the Synthesis of Thiosquaramides Michael Rombola and Viresh H. Rawal* Department of Chemistry, The University of Chicago 5735 South Ellis Avenue Chicago, Illinois, 60637, United States vrawal@uchicago.edu Supporting information. General Thin-layer chromatography (TLC) was performed using Whatman silica gel 60 Å F254 plates (250 µm) with F-254 fluorescent indicator and visualized by UV fluorescence quenching, ceric ammonium molybdate, or potassium permanganate staining. SiliCycle SiliaFlash P60 silica gel (particle size 40 63 µm) was used for flash chromatography. MR spectra were measured on Bruker DRX and DMX spectrometers at 500 MHz for 1 H spectra and 125 MHz for 13 C spectra, respectively, and calibrated to either TMS (δ = 0 for 1 H), residual CHCl 3 (δ = 7.26 for 1 H and δ = 77.23 for 13 C), residual DMSO (δ = for 1 H and δ = 39.51 for 13 C), or residual CD 3 C (δ = for 1 H and δ = 1.39 for 13 C). Splitting patterns are reported as apparent. Mass spectral data was measured on Agilent technologies 6224 TOF LC/MS. Infrared spectra were recorded on a icolet is5 FT-IR spectrometer and are reported in frequency of absorption (cm 1 ) using abr salt plates using a thin film. All reactions were run under a nitrogen atmosphere unless otherwise noted. S1

Synthesis of compounds 3,4-bis(pentan-3-yloxy)cyclobut-3-ene-1,2-dione (1b). A suspension of squaric acid (3.42 g, 30 mmol, 1.0 equiv) in PhMe (21 ml) was treated with 3-pentanol (21 ml, 195 mmol, 6.5 equiv) and the resulting mixture was refluxed in a Dean-Stark apparatus. After 24 h, the reaction mixture was concentrated. Purification by flash column chromatography (CH 2 Cl 2 3% MeOH in CH 2 Cl 2 ) afforded 1b (5.92 g, 23.3 mmol, 78%) as an oil. 1 H MR (500 MHz, CDCl 3 ): δ = 5.03 (p, J = 6.1 Hz, 2H), 1.76 (m, 8H), 0.98 (t, J = 7.4 Hz, 12H). 13 C MR (125 MHz, CDCl 3 ): δ = 189.7, 184.5, 88.8, 27.5, 9.5. HRMS (ESI) cald for C 14 H 22 O 4 (M+H) + : 255.1591. Found: 255.1593. IR (film): 1812, 1733, 1596 cm -1 3,4-bis(cyclopentyloxy)cyclobut-3-ene-1,2-dione (1c). A suspension of squaric acid (4.56 g, 40 mmol, 1.0 equiv) in PhMe (30 ml) was treated with cyclopentanol (24 ml, 264 mmol, 6.5 equiv) and the resulting mixture was refluxed with Dean-Stark apparatus. After 7 h, the reaction mixture was concentrated. Purification by flash column chromatography (CH 2 Cl 2 1% MeOH in CH 2 Cl 2 2% MeOH in CH 2 Cl 2 ) gave a brown solid, which was washed with ice-cold hexanes to afford 1c (7.31 g, 29.2 mmol, 73%) as a white solid. 1 H MR (500 MHz, CDCl 3 ): δ = 5.54 (tt, J = 5.3, 2.5 Hz, 2H), 1.96 (m, 8H), 1.81 (m, 4H), 1.68 (m, 4H). 13 C MR (125 MHz, CDCl 3 ): δ = 189.8, 184.3, 88.1, 33.9, 23.8. HRMS (ESI) cald for C 14 H 18 O 4 (M+H) + : 251.1278. Found: 251.1283. IR (film): 1721 cm - 1. mp 60-62 C 3,4-bis(pentan-3-yloxy)cyclobut-3-ene-1,2-dithione (2b). To a solution of Lawesson s reagent (4.33 g, 10.7 mmol, 1.0 equiv) in dry CH 2 Cl 2 (45 ml) was added 1b (2.71 g, 10.7 mmol, 1.0 equiv). The reaction was stirred for 46 h, during which time dry CH 2 Cl 2 was added as needed to maintain constant volume. The reaction mixture was then gravity filtered, concentrated to roughly half the original volume, and immediately loaded onto column. Quickly eluting with 1:1 hexanes:ch 2 Cl 2 afforded 2b (2.46 g, 8.6 mmol, 80%) as a red oil. 1 H MR (500 MHz, CDCl 3 ): δ = 5.69 (m, 2H), 1.82 (m, 8H), 1.01 (t, J = 7.4 Hz, 12H). 13 C MR (125 MHz, CDCl 3 ): δ = 218.6, 186.6, 89.2, 27.6, 9.4. HRMS (ESI) cald for C 14 H 22 O 2 S 2 (M+H) + : 287.1134. Found: 287.1119. IR (film): 1648 cm -1 S2

3,4-bis(cyclopentyloxy)cyclobut-3-ene-1,2-dithione (2c). To a solution of Lawesson s reagent (3.70 g, 9.15 mmol, 1.0 equiv) in dry CH 2 Cl 2 (40 ml) was added 1c (2.29 g, 9.15 mmol, 1.0 equiv). The reaction was stirred for 37 h, during which time dry CH 2 Cl 2 was added as needed to maintain constant volume. The reaction mixture was then gravity filtered, concentrated to roughly half the original volume, and immediately loaded onto column. Quickly eluting with 1:1 hexanes:ch 2 Cl 2 afforded 2c (1.83 g, 6.48 mmol, 71%) as an amorphous orange solid. 1 H MR (500 MHz, CDCl 3 ): δ = 6.06 (m, 2H), 2.02 (m, 8H), 1.86 (m, 4H), 1.70 (m, 4H). 13 C MR (125 MHz, CDCl 3 ): δ = 218.8, 186.3, 88.9, 34.4, 23.9. HRMS (ESI) cald for C 14 H 18 O 2 S 2 (M+H) + : 283.0821. Found: 283.0733. IR (film): 1648 cm -1 2,3-bis(cyclopentyloxy)-4-thioxocyclobut-2-en-1-one (3b). To a solution of Lawesson s reagent (737 mg, 1.82 mmol, 0.5 equiv) in dry CH 2 Cl 2 (8 ml) was added 1c (911 mg, 3.64 mmol, 1.0 equiv). After 25 h, the reaction mixture was concentrated to roughly half the original volume and immediately loaded onto column. Quickly eluting with 1:1 hexanes:ch 2 Cl 2 CH 2 Cl 2 afforded 3b (659 mg, 2.47 mmol, 68%) as a pink solid that decomposes under ambient conditions within several days. 1 H MR (500 MHz, CDCl 3 ): δ = 5.88 (m, 1H), 5.58 (tt, J = 5.3, 2.6 Hz, 1H), 1.99 (m, 8H), 1.83 (m, 4H), 1.70 (m, 4H). 13 C MR (125 MHz, CDCl 3 ): δ = 223.2, 187.7, 185.2, 185.0, 89.3, 88.0, 34.1, 33.9, 23.9, 23.7. HRMS (ESI) cald for C 14 H 18 O 3 S (M+a) + : 289.0869. Found: 289.0841. IR (film): 1780, 1612 cm -1. mp 71-73 C 3,4-bis(benzylamino)cyclobut-3-ene-1,2-dithione (4a). To a solution of 2c (141 mg, 0.50 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1 ml) was added benzylamine (115 µl, 1.05 mmol, 2.1 equiv) at 0 C and the resulting solution was stirred for 5 min at that temperature, then 1.5 h at room temperature. 1 ml hexanes was then added and the suspension filtered to afford 4a (139 mg, 0.43 mmol, 86%) as an amorphous yellow solid. The compound exists as three rotamers in DMSO at room temperature in a ratio of 26:9:2. Major rotamer: 1 H MR (500 MHz, DMSO-d 6 ): δ = 8.81 (t, J = 6.2 Hz, 2H), 7.34 (m, 10H), 5.31 (d, J = 6.4 Hz, 4H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 204.1, 170.0, 137.4, 128.7, 128.0, 127.7, 46.0. HRMS (ESI) cald for C 18 H 16 2 S 2 (M+H) + : 325.0828. Found: 325.0826. IR (film): 1708, 1569 cm -1 S3

3,4-bis(cyclohexylamino)cyclobut-3-ene-1,2-dithione (4b). To a solution of 2c (141 mg, 0.50 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1 ml) was added cyclohexylamine (120 µl, 1.05 mmol, 2.1 equiv) at 0 C and the resulting solution was stirred for 5 min at that temperature, then 2 h at room temperature. 1 ml hexanes was then added and the suspension filtered to afford 4b (92 mg, 0.30 mmol, 60%) as an amorphous yellow solid. Major rotamer: 1 H MR (500 MHz, DMSO-d 6 ): δ = 8.40 (d, J = 8.6 Hz, 2H), 4.70 (m, 2H), 1.05-2.10 (m, 20H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 203.5, 169.6, 51.6, 33.5, 24.5, 23.8. HRMS (ESI) cald for C 16 H 24 2 S 2 (M+H) + : 309.1454. Found: 309.1451. IR (film): 1705, 1567 cm -1 3,4-bis(tert-butylamino)cyclobut-3-ene-1,2-dithione (4c). To a solution of 2c (141 mg, 0.50 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1 ml) was added tert-butyl amine (110 µl, 1.05 mmol, 2.1 equiv) at 0 C and the resulting solution was stirred for 5 min at that temperature, then 2 h at room temperature. 1 ml hexanes was then added and the suspension filtered to afford 4c (39 mg, 0.15 mmol, 30%) as an amorphous yellow solid. 1 H MR (500 MHz, DMSO-d 6 ): δ = 8.70 (s, 2H), 1.60 (s, 18H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 203.9, 172.1, 54.6, 31.0. HRMS (ESI) cald for C 12 H 20 2 S 2 (M+H) + : 257.1141. Found: 257.1142. IR (film): 1682, 1546 cm -1 3,4-bis(phenylamino)cyclobut-3-ene-1,2-dithione (4d). To a solution of 2c (94 mg, 0.33 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1 ml) was added aniline (90 µl, 0.99 mmol, 3.0 equiv) and the resulting solution stirred for 20 h, during which time a yellow precipitate appeared. 1 ml hexanes was then added and the suspension filtered to afford 4d (76 mg, 0.26 mmol, 79%) as an amorphous yellow solid. Analytical data matched previously reported values: 1 1 H MR (500 MHz, DMSO-d 6 ): δ = 10.86 (s, 2H), 6.90-7.40 (m, 10H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 207.9, 169.4, 136.9, 128.5, 125.3, 122.1. S4

3,4-bis((3,5-dimethoxyphenyl)amino)cyclobut-3-ene-1,2-dithione (4e). To a solution of 2c (85 mg, 0.30 mmol, 1.0 equiv) in dry CH 2 Cl 2 (0.7 ml) was added 3,5-dimethoxyaniline (97 mg, 0.63 mmol, 2.1 equiv) and the resulting solution was stirred for 40 h, during which time a yellow precipitate formed. 1 ml hexanes was then added and the suspension filtered to afford 4e (95 mg, 0.23 mmol, 77%) as an amorphous yellow solid. 1 H MR (500 MHz, CD 3 C): δ = 8.72 (s, 2H), 6.31 (s, 4 H), 6.12 (s, 2H), 3.68 (s, 12H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 169.7, 160.2, 139.0, 100.5, 97.7, 55.1. HRMS (ESI) cald for C 20 H 20 2 O 4 S 2 (M+H) + : 417.0937. Found: 417.0939. IR (film): 1694, 1597, 1540 cm -1 3,4-bis((3,5-bis(trifluoromethyl)phenyl)amino)cyclobut-3-ene-1,2-dithione (4f). To a solution of 2c (141 mg, 0.5 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1.5 ml) was added 3,5-bis(trifluoromethyl)aniline (0.31 ml, 2.0 mmol, 4.0 equiv) and the resulting solution was stirred for 4 days, during which time the solution turned dark and a yellow precipitate formed. About 2 ml hexanes was then added and the suspension filtered to afford 4f (145 mg, 0.26 mmol, 52%) as an amorphous yellow solid. Spectroscopic data for 4f was consistent with that of 4d and 4e, but differed from previously reported values. 1 1 H MR (500 MHz, CD 3 C): δ = 9.16 (s, 2H), 7.69 (s, 2H), 7.62 (s, 1H). 13 C MR (125 MHz, CD 3 C): δ = 213.3, 170.2, 139.9, 133.0 (q, J = 33.8 Hz), 125.0, 123.2, 122.9, 120.0. HRMS (ESI) cald for C 20 H 8 F 12 2 S 2 (M+H) + : 569.0010. Found: 568.9991. IR (film): 1683, 1625, 1558 cm -1 3-(benzylamino)-4-(cyclopentyloxy)cyclobut-3-ene-1,2-dithione (5a). To a solution of 2c (237 mg, 0.84 mmol, 1.2 equiv) in dry CH 2 Cl 2 (2 ml) was added benzylamine (76 µl, 0.70 mmol, 1.0 equiv) at 0 C and the resulting solution was stirred for 15 min at that temperature, then 15 min at room temperature. The solution was then loaded directly onto column and quickly eluted with CH 2 Cl 2 to afford 5a (166 mg, 0.55 mmol, 79%) as an amorphous yellow solid. The compound exists as two rotamers in DMSO at room temperature in a ratio of 0.55:0.33. Major rotamer: 1 H MR (500 MHz, DMSO-d 6 ): δ = 10.26 (t, J = 6.2 Hz, 1H), 7.35 (m, 5H), 6.37 (m, 1H), 4.56 (d, J = 6.5 Hz, 2H), 1.81-2.08 (m, 4H), 1.53-1.81 (m, 4H). Minor rotamer: 1 H MR (500 MHz, DMSO-d 6 ): δ = 10.15 (t, J = 6.3 Hz, 1H), 7.35 (m, 5H), 6.32 (m, 1H), 5.19 (d, J = 6.5 Hz, 1H), 1.81-2.08 (m, 4H), 1.53-1.81 (m, S5

4H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 217.7, 217.4, 205.6, 182.8, 182.2, 175.2, 172.7, 137.2, 137.2, 128.6, 128.6, 127.9, 127.7, 127.6, 127.4, 87.3, 87.2, 47.9, 45.9, 33.7, 23.2, 23.0. HRMS (ESI) cald for C 16 H 17 OS 2 (M+H) + : 304.0824. Found: 304.0807. IR (film): 1691, 1513 cm -1 3-(cyclopentyloxy)-4-(pyrrolidin-1-yl)cyclobut-3-ene-1,2-dithione (5b). To a solution of 2c (169 mg, 0.60 mmol, 1.2 equiv) in dry CH 2 Cl 2 (1.5 ml) was added pyrrolidine (42 µl, 0.50 mmol, 1.0 equiv) at 0 C and the resulting solution was stirred for 20 min at that temperature, then 30 min at room temperature. The solution was then loaded directly onto column and quickly eluted with 1:1 hexanes:ch 2 Cl 2 CH 2 Cl 2 to afford 5b (84 mg, 0.31 mmol, 62%) as an amorphous yellow solid. 1 H MR (500 MHz, CDCl 3 ): δ = 6.45 (m, 1H), 4.17 (m, 2H), 3.69 (m, 2H), 1.92-2.14 (m, 8H), 1.65-1.84 (m, 4H). 13 C MR (125 MHz, CDCl 3 ): δ = 218.0, 205.5, 182.3, 171.3, 87.8, 50.1, 49.5, 34.5, 25.3, 24.7, 23.9. HRMS (ESI) cald for C 13 H 17 OS 2 (M+H) + : 268.0824. Found: 268.0825. IR (film): 1708, 1506 cm -1 3-(cyclopentyloxy)-4-(phenylamino)cyclobut-3-ene-1,2-dithione (5c). To a solution of 2c (339 mg, 1.2 mmol, 1.2 equiv) in dry CH 2 Cl 2 (3 ml) was added aniline (91 µl, 1.0 mmol, 1.0 equiv) and the resulting solution was stirred for 14 h, during which time a yellow precipitate appeared (diaryl thiosquaramide byproduct). The suspension was then loaded directly onto column and quickly eluted with 1:1 hexanes:ch 2 Cl 2 CH 2 Cl 2 to afford 5c (150 mg, 0.52 mmol, 52%) as an orange solid. 1 H MR (500 MHz, DMSO-d 6 ): δ = 11.59 (s, 1H), 7.40 (m, 4H), 7.26 (m, 1H), 6.47 (s, 1H), (s, 4H), 1.66 (s, 4H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 217.5, 208.0, 181.6, 172.5, 136.3, 128.7, 126.0, 122.2, 87.8, 33.7, 23.0. HRMS (ESI) cald for C 15 H 15 OS 2 (M+H) + : 290.0668. Found: 290.0655. IR (film): 1674, 1598, 1520 cm -1. mp 108-112 C (decomp) 3-(pentan-3-yloxy)-4-(phenylamino)cyclobut-3-ene-1,2-dithione (5d). To a solution of 2b (1.00 g, 3.49 mmol, 1.2 equiv) in dry CH 2 Cl 2 (2 ml) was added aniline (0.26 ml, 2.90 mmol, 1.0 equiv) and the resulting solution was stirred for 13 h. The solution was then loaded directly onto column and quickly eluted with 1:1 hexanes:ch 2 Cl 2 CH 2 Cl 2 1% MeOH in CH 2 Cl 2 to afford 5d (499 mg, 1.71 mmol, 59%) as an amorphous orange solid. 1 H MR (500 MHz, DMSO-d 6 ): δ = 11.63 (s, 1H), S6

7.40 (m, 4H), 7.28 (m, 1H), 6.15 (m, 1H), 1.75 (m, 4H), 0.91 (s, 6H). 13 C MR (125 MHz, DMSOd 6 ): δ = 217.4, 207.7, 182.1, 172.3, 136.3, 128.8, 126.1, 122.2, 87.0, 26.3, 8.7. HRMS (ESI) cald for C 15 H 17 OS 2 (M+H) + : 292.0824. Found: 292.0826. IR (film): 1674, 1598, 1520 cm -1 3-(cyclopentyloxy)-4-((4-methoxyphenyl)amino)cyclobut-3-ene-1,2-dithione (5e). To a solution of 2c (220 mg, 0.79 mmol, 1.2 equiv) in dry CH 2 Cl 2 (1.5 ml) was added p-anisidine (81 mg, 0.66 mmol, 1.0 equiv) and the resulting solution was stirred for 3 h. The solution was then loaded directly onto column and quickly eluted with 1:1 hexanes:ch 2 Cl 2 1:2 hexanes:ch 2 Cl 2 CH 2 Cl 2 to afford 5e (164 mg, 0.51 mmol, 77%) as an orange solid. 1 H MR (500 MHz, DMSO-d 6 ): δ = 11.49 (s, 1H), 7.33 (d, J = 8.8 Hz, 2H), 6.95 (d, J = 8.8 Hz, 2H), 6.46 (s, 1H), 3.76 (s, 3H), 1.93 (s, 4H), 1.65 (m, 4H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 217.7, 206.8, 181.6, 172.1, 157.6, 129.3, 123.7, 113.9, 87.6, 55.4, 33.7, 23.0. HRMS (ESI) cald for C 16 H 17 O 2 S 2 (M+H) + : 320.0773. Found: 320.0745. IR (film): 1680, 1511 cm -1. mp 120-124 C (decomp) 3-((3,5-bis(trifluoromethyl)phenyl)amino)-4-(cyclopentyloxy)cyclobut-3-ene-1,2-dithione (5f). To a solution of 2c (261 mg, 0.92 mmol, 1.2 equiv) in dry CH 2 Cl 2 (1 ml) was added 3,5- bis(trifluoromethyl)aniline (120 µl, 0.77 mmol, 1.0 equiv) and the resulting solution was stirred for 16 h. The solution was then loaded directly onto column and quickly eluted with 1:1 hexanes:ch 2 Cl 2 1:2 hexanes:ch 2 Cl 2 CH 2 Cl 2 to afford 5f (218 mg, 0.51 mmol, 66%) as an orange solid. 1 H MR (500 MHz, DMSO-d 6 ): δ = 11.85 (s, 1H), 8.14 (s, 2H), 7.97 (s, 1H), 6.46 (s, 1H), 1.98 (m, 4H), 1.62 (m, 4H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 216.5, 210.1, 181.9, 173.0, 138.6, 130.9 (q, J = 33.4 Hz), 126.2, 124.0, 122.2, 121.9, 119.7, 118.4, 88.4, 33.7, 23.2. HRMS (ESI) cald for C 17 H 13 F 6 OS 2 (M+H) + : 426.0416. Found: 426.0389. IR (film): 1672, 1534 cm -1. mp 120-126 C (decomp) Synthesis of hydrochloride salts of catalysts 6a-g. Due to zwitterionic and rotameric forms of catalysts 6a-g, their HCl salts were used for MR characterization. Representative example: To solid thiosquaramide catalyst was added 1.0 M hydrochloric acid in Et 2 O (0.4 ml, 0.4 mmol, 4.0 equiv) and the resulting suspension stirred for 2 min at room temperature. 0.6 ml CH 2 Cl 2 was then added, followed by 1.0 M hydrochloric acid in Et 2 O (0.2 ml, 0.2 mmol, 2.0 equiv) and the suspension stirred for another 2 min at room temperature. About 1 ml hexanes was then added and the suspension filtered to afford the HCl salts of thiosquaramide catalysts 6a-g which were used for MR characterization. S7

S S Ph H H 6a 3-(benzylamino)-4-(((1R,2R)-2-(piperidin-1-yl)cyclohexyl)amino)cyclobut-3-ene-1,2-dithione (6a). To a solution of 5a (156 mg, 0.51 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1.5 ml) was added (1R,2R)-2- (piperidin-1-yl)cyclohexan-1-amine 2 (93 mg, 0.51 mmol, 1.0 equiv) at 0 C and the resulting solution was stirred for 1 h at that temperature, then 0.5 h at room temperature. Hexanes was then added and the resulting solution concentrated to give a solid, which was washed with pentane to afford 6a (185 mg, 0.46 mmol, 90%) as an orange solid. Analytical data matched previously reported values. 2 MR characterization for 6a.HCl: 1 H MR (500 MHz, CD 3 C): δ = 10.04 (s, 1H), 9.20 (s, 1H), 8.53 (s, 1H), 7.45 (d, J = 7.3 Hz, 2H), 7.39 (t, J = 7.4 Hz, 2H), 7.32 (t, J = 7.2 Hz, 1H), 5.35 (m, 2H), 4.90 (m, 1H), 3.48 (d, J = 11.3 Hz, 1H), 3.39 (t, J = 10.6 Hz, 1H), 3.23 (m, 2H), 2.91 (m, 1H), 2.45 (d, J = 13.4 Hz, 1H), 1.89 (m, 4H), 1.32-1.82 (m, 9H). 13 C MR (125 MHz, CD 3 C): δ = 207.9, 205.5, 173.0, 172.1, 138.8, 129.9, 129.0, 128.9, 68.8, 54.0, 53.6, 48.2, 47.9, 36.6, 24.9, 24.5, 24.3, 24.2, 24.0, 22.5. 3-(phenylamino)-4-(((1R,2R)-2-(piperidin-1-yl)cyclohexyl)amino)cyclobut-3-ene-1,2-dithione (6b). To a solution of 5c (80 mg, 0.28 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1 ml) was added (1R,2R)-2- (piperidin-1-yl)cyclohexan-1-amine 2 (51 mg, 0.28 mmol, 1.0 equiv) at 0 C and the resulting solution was stirred for 1 h at that temperature, then 1 h at room temperature. The solution was then loaded directly onto column and quickly eluted with CH 2 Cl 2 1% MeOH in CH 2 Cl 2 3% MeOH in CH 2 Cl 2 5% MeOH in CH 2 Cl 2 to afford 6b (104 mg, 0.27 mmol, 96%) as an orange solid. MR characterization for 6b.HCl: 1 H MR (500 MHz, CD 3 C): δ = 11.05 (s, 1H), 9.99 (s, 1H), 8.48 (s, 1H), 7.77 (d, J = 7.6 Hz, 2H), 7.38 (t, J = 7.9 Hz, 2H), 7.21 (t, J = 7.4 Hz, 1H), 4.95 (s, 1H), 3.47 (m, 2H), 3.27 (m, 2H), 2.94 (m, 1H), 2.46 (d, J = 13.5 Hz, 1H), 1.88-2.21 (buried, 5H), 1.80 (m, 3H), 1.60 (m, 2H), 1.42 (m, 3H). 13 C MR (125 MHz, CD 3 C): δ = 208.5, 207.0, 173.0, 171.2, 137.7, 129.9, 126.5, 123.1, 68.9, 54.1, 53.8, 48.2, 36.5, 24.8, 24.5, 24.3, 24.2, 24.0, 22.5. HRMS (ESI) cald for C 21 H 27 3 S 2 (M+H) + : 386.1719. Found: 386.1719. IR (film): 1691, 1598, 1559 cm -1. mp 130-146 C (decomp) S8

3-((4-methoxyphenyl)amino)-4-(((1R,2R)-2-(piperidin-1-yl)cyclohexyl)amino)cyclobut-3-ene-1,2- dithione (6c). To a solution of 5e (109 mg, 0.34 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1 ml) was added (1R,2R)-2-(piperidin-1-yl)cyclohexan-1-amine 2 (62 mg, 0.34 mmol, 1.0 equiv) at 0 C and the resulting solution was stirred for 0.5 h at that temperature, then 1.5 h at room temperature. The solution was then loaded directly onto column and quickly eluted with CH 2 Cl 2 1% MeOH in CH 2 Cl 2 3% MeOH in CH 2 Cl 2 5% MeOH in CH 2 Cl 2 to afford 6c (126 mg, 0.30 mmol, 88%) as an orange solid. MR characterization for 6c.HCl: 1 H MR (500 MHz, CD 3 C): δ = 10.99 (s, 1H), 9.93 (s, 1H), 8.47 (s, 1H), 7.67 (d, J = 8.1 Hz, 2H), 6.93 (d, J = 8.9 Hz, 2H), 4.95 (m, 1H), 3.79 (s, 3H), 3.46 (m, 2H), 3.25 (m, 2H), 2.94 (q, J = 11.5 Hz, 1H), 2.46 (d, J = 12.7 Hz, 1H), 1.88-2.21 (buried, 5H), 1.80 (m, 3H), 1.60 (m, 2H), 1.42 (m, 3H). 13 C MR (125 MHz, CD 3 C): δ = 207.1, 207.0, 170.7, 158.8, 130.7, 124.7, 115.0, 68.9, 56.3, 54.1, 53.7, 48.3, 36.4, 24.8, 24.4, 24.3, 24.2, 23.9, 22.5. HRMS (ESI) cald for C 22 H 29 3 OS 2 (M+H) + : 416.1825. Found: 416.1823. IR (film): 1694, 1606 cm -1. mp 118-138 C (decomp) 3-((3,5-bis(trifluoromethyl)phenyl)amino)-4-(((1R,2R)-2-(piperidin-1-yl)cyclohexyl)amino)cyclobut- 3-ene-1,2-dithione (6d). To a solution of 5f (213 mg, 0.50 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1 ml) was added (1R,2R)-2-(piperidin-1-yl)cyclohexan-1-amine 2 (91 mg, 0.50 mmol, 1.0 equiv) at 0 C and the resulting solution was stirred for 1 h at that temperature, then 0.5 h at room temperature. About 5 ml hexanes was then added. The suspension was then filtered and washed with ice-cold 4:1 hexanes:ch 2 Cl 2 to afford 6d (187 mg, 0.36 mmol, 72%) as an orange-red solid. MR characterization for 6d.HCl: 1 H MR (500 MHz, CD 3 C): δ = 11.60 (s, 1H), 9.98 (s, 1H), 8.42 (s, 2H), 8.33 (s, 1H), 7.75 (s, 1H), 4.93 (s, 1H), 3.47 (m, 2H), 3.32 (m, 2H), 2.96 (m, 1H), 2.44 (m, 1H), 2.17 (m, 3H), 1.90 (m, 2H), 1.80 (m, 3H), 1.52-1.73 (m, 2H), 1.34-1.52 (m, 2H). 13 C MR (125 MHz, CD 3 C): δ = 210.9, 206.9, 173.0, 171.3, 139.7, 132.6 (q, J = 33.3 Hz), 125.4, 123.3, 122.5, 68.9, 54.1, 54.0, 48.4, 36.4, 24.8, 24.4, 24.2, 24.2, 24.0, 22.4. HRMS (ESI) cald for C 23 H 25 F 6 3 S 2 (M+H) + : 522.1467. Found: 522.1472. IR (film): 1688, 1568 cm -1. mp 138-144 C (decomp) 3-(((1R,2R)-2-(dimethylamino)cyclohexyl)amino)-4-(phenylamino)cyclobut-3-ene-1,2-dithione (6e). To a solution of 5c (80 mg, 0.28 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1 ml) was added (1R,2R)- 1, 1 - dimethylcyclohexane-1,2-diamine 3 (44 µl, 0.28 mmol, 1.0 equiv) at 0 C and the resulting solution was stirred for 1 h at that temperature, then 1 h at room temperature. The solution was then loaded directly onto column and quickly eluted with CH 2 Cl 2 1% MeOH in CH 2 Cl 2 3% MeOH in S9

CH 2 Cl 2 5% MeOH in CH 2 Cl 2 to afford 6e (80 mg, 0.23 mmol, 82%) as a yellow solid. MR characterization for 6e.HCl: 1 H MR (500 MHz, CD 3 C): δ = 11.51 (s, 1H), 10.35 (s, 1H), 8.42 (s, 1H), 7.79 (d, J = 6.5 Hz, 2H), 7.38 (t, J = 7.9 Hz, 2H), 7.22 (t, J = 7.4 Hz, 1H), 5.21 (s, 1H), 3.39 (td, J = 11.9, 3.5 Hz, 1H), 2.87 (d, J = 3.9 Hz, 3H), 2.78 (d, J = 4.5 Hz, 3H), 2.32 (m, 1H), 2.10 (m, 1H), 1.89 (m, 1H), 1.79 (m, 1H), 1.48-1.69 (m, 2H), 1.33-1.46 (m, 2H). 13 C MR (125 MHz, CD 3 C): δ = 208.3, 206.8, 173.4, 171.3, 137.6, 129.9, 126.6, 123.3, 68.9, 53.7, 43.6, 38.0, 35.4, 24.5, 24.4, 23.4. HRMS (ESI) cald for C 18 H 23 3 S 2 (M+H) + : 346.1406. Found: 346.1404. IR (film): 1691, 1598, 1559 cm -1. mp 148-158 C (decomp) 3-((3,5-bis(trifluoromethyl)phenyl)amino)-4-(((1R,2R)-2-(dimethylamino)cyclohexyl)amino)cyclobut- 3-ene-1,2-dithione (6f). To a solution of 5f (213 mg, 0.50 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1.5 ml) was added (1R,2R)- 1, 1 -dimethylcyclohexane-1,2-diamine 3 (78 µl, 0.50 mmol, 1.0 equiv) at 0 C and the resulting solution was stirred for 1 h at that temperature, then 0.5 h at room temperature. About 2 ml hexanes was then added and the suspension filtered to afford 6f (182 mg, 0.38 mmol, 76%) as a yellow solid. MR characterization for 6f.HCl: 1 H MR (500 MHz, CD 3 C): δ = 12.06 (s, 1H), 10.32 (s, 1H), 8.47 (s, 2H), 8.25 (s, 1H), 7.74 (s, 1H), 5.17 (m, 1H), 3.45 (td, J = 11.8, 3.7 Hz, 1H), 2.91 (s, 3H), 2.78 (s, 3H), 2.31 (m, 1H), 2.13 (m, 1H), 1.90 (m, 1H), 1.80 (m, 1H), 1.51-1.68 (m, 2H), 1.33-1.50 (m, 2H). 13 C MR (125 MHz, CD 3 C): δ = 210.8, 206.7, 173.5, 171.4, 139.9, 132.7 (q, J = 33.3 Hz), 125.5, 123.3, 122.6, 69.1, 53.9, 43.6, 38.1, 35.5, 24.4, 24.4, 23.5. HRMS (ESI) cald for C 20 H 21 F 6 3 S 2 (M+H) + : 482.1154. Found: 482.1157. IR (film): 1684, 1568 cm - 1. mp 156-160 C (decomp) Quinine-derived diamine QA was synthesized according to the literature. 4 S10

3-((3,5-bis(trifluoromethyl)phenyl)amino)-4-(((S)-(6-methoxyquinolin-4-yl)((1S,2S,4S,5R)-5- vinylquinuclidin-2-yl)methyl)amino)cyclobut-3-ene-1,2-dithione (6g). To a solution of 5f (85 mg, 0.20 mmol, 1.0 equiv) in dry CH 2 Cl 2 (1 ml) was added a solution of diamine QA in dry CH 2 Cl 2 (0.5 M, 0.4 ml, 1.0 equiv) at 0 C and the resulting solution was stirred for 0.5 h at that temperature, then 3 h at room temperature. About 4 ml hexanes was then added. The suspension was filtered and washed with ice-cold 4:1 hexanes:ch 2 Cl 2 to afford 6g (93 mg, 0.14 mmol, 70%) as an amorphous yellow solid. MR characterization for 6g.2HCl: 1 H MR (500 MHz, CD 3 C): δ = 12.36 (s, 1H), 11.72 (s, 1H), 9.82 (s, 1H), 9.03 (s, 1H), 8.61 (s, 1H), 8.55 (d, J = 8.8 Hz, 1H), 8.37 (s, 2H), 8.06 (s, 1H), 7.71 (m, 2H), 7.53 (s, 1H), 5.85 (m, 1H), 5.18 (d, J = 16.5 Hz, 1H), 5.12 (d, J = 10.0 Hz, 1H), 4.38 (s, 1H), 4.15 (s, 3H), 4.15 (buried, 1H), 3.70 (m, 1H), 3.34 (s, 2H), 2.81 (s, 1H), 1.54-2.09 (m, 5H), 1.14 (s, 1H). 13 C MR (125 MHz, CD 3 C): δ = 209.6, 207.2, 171.6, 168.9, 162.0, 141.9, 139.4, 138.7, 138.5, 136.6, 132.7 (q, J = 33.8 Hz), 129.9, 128.8, 125.3, 125.3, 123.2, 122.9, 121.0, 119.3, 117.8, 103.9, 58.1, 55.0, 43.7, 37.4, 27.5, 24.6, 24.1. HRMS (ESI) cald for C 32 H 28 F 6 4 OS 2 (M+H) + : 663.1681. Found: 663.1680. IR (film): 1681, 1622, 1566, 1510 cm -1 2-(tert-butylamino)-3-(cyclopentyloxy)-4-thioxocyclobut-2-en-1-one (7). To a solution of 3b (278 mg, 1.04 mmol, 1.2 equiv) in dry CH 2 Cl 2 (2 ml) was added tert-butyl amine (91 µl, 0.87 mmol, 1.0 equiv) at 0 C and the resulting solution was stirred for 3 h at that temperature. The solution was then loaded directly onto column and quickly eluted with CH 2 Cl 2 5% MeOH in CH 2 Cl 2 to afford 7 (186 mg, 0.73 mmol, 84%) as an amorphous yellow solid. The compound exists as two rotamers in DMSO at room temperature in a ratio of 0.54:0.40. Major rotamer: 1 H MR (500 MHz, DMSO-d 6 ): δ = 9.43 (s, 1H), 6.32 (m, 1H), 1.82-2.06 (m, 4H), 1.55-1.82 (m, 4H), 1.38 (s, 9H); Minor rotamer: 1 H MR (500 MHz, DMSO-d 6 ): δ = 9.74 (s, 1H), 6.50 (m, 1H), 1.82-2.06 (m, 4H), 1.55-1.82 (m, 4H), 1.34 (s, 9H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 205.5, 203.4, 184.6, 183.2, 181.0, 178.5, 176.8, 175.6, 85.7, 54.4, 53.8, 33.7, 33.7, 29.7, 29.5, 23.3, 23.1. HRMS (ESI) cald for C 13 H 19 O 2 S (M+H) + : 254.1209. Found: 254.1212. IR (film): 1765, 1633 cm -1 S11

2-(tert-butylamino)-3-(((1R,2R)-2-(dimethylamino)cyclohexyl)amino)-4-thioxocyclobut-2-en-1-one (8). To a solution of 7 (230 mg, 0.91 mmol, 1.0 equiv) in dry CH 2 Cl 2 (0.5 ml) was added (1R,2R)- 1, 1 -dimethylcyclohexane-1,2-diamine 3 (142 µl, 0.91 mmol, 1.0 equiv) at room temperature. A precipitate formed within 30 min, at which point an additional 1 ml dry CH 2 Cl 2 was added to facilitate stirring. After stirring an additional 24 h, about 2 ml hexanes was added and the suspension filtered to afford 8 (162 mg, 0.52 mmol, 57%) as an amorphous yellow solid. 1 H MR (500 MHz, DMSO-d 6 ): δ = 8.46 (s, 1H), 7.83 (d, J = 8.5 Hz, 1H), 4.82 (m, 1H), 2.35 (m, 1H), 2.20 (s, 6H), 2.08 (m, 1H), 1.84 (m, 1H), 1.73 (m, 1H), 1.62 (m, 1H), 1.42 (s, 9H), 1.11-1.30 (m, 4H). 13 C MR (125 MHz, DMSO-d 6 ): δ = 200.1, 178.5, 171.8, 169.2, 65.8, 53.7, 53.0, 38.9-40.3 (buried x2), 35.9, 29.9, 24.3, 24.1, 21.1. HRMS (ESI) cald for C 16 H 27 3 OS (M+H) + : 310.1948. Found: 310.1947. IR (film): 1756, 1594, 1538 cm -1 Squaramides 12a, 5 12b, 6 12c, 6 12d, 2 and 12e 7 are known. O O OH + Ph O O OEt catalyst (10 mol %) CH 2 Cl 2, 1 h, rt O O OH * Ph O O OEt 9 10 11 Asymmetric Michael addition of lawsone (9) to β,γ-unsaturated α-oxo ester 10 8 catalyzed by an oxoor thiosquaramide catalyst. Representative example: To a solution of lawsone (17.4 mg, 0.10 mmol, 1.0 equiv) and catalyst (0.01 mmol, 10 mol %) in CH 2 Cl 2 (0.5 ml) was added α-oxo ester 10 (22.5 mg, 0.11 mmol, 1.1 equiv) and the resulting solution was stirred for 1 h at room temperature. About half of the solution was then loaded directly onto a preparatory TLC plate and eluted with 1:2 EtOAc:hexanes to afford pure 11 for HPLC analysis. The remaining solution was concentrated and conversion measured by 1 H-MR spectroscopy. MR data matched previously reported values. 9 HPLC conditions: Daicel Chiralpak IC column, 80:20 hexanes:iproh, 1.0 ml/min, λ = 250 nm, t minor = 15.3 min, t major = 19.7 min. TBSO Ph + Ph Ph catalyst (20 mol %) PhMe, 12 h, rt TBSO Ph Ph Ph 13 14 15 Aza-Diels-Alder reaction of 2-siloxydiene 13 10 with -benzylideneaniline 14 catalyzed by thiosquaramide 4f, oxosquaramide 12e, or Schreiner s thiourea catalyst. Representative example: To a solution of -benzylideneaniline 14 (36 mg, 0.20 mmol, 1.0 equiv) and catalyst (0.04 mmol, 20 S12

mol %) in dry PhMe (1 ml) or dry CH 2 Cl 2 (1 ml) was added 2-siloxydiene 13 (63 mg, 0.24 mmol, 1.2 equiv) and the resulting reaction mixture was stirred for 12 h at room temperature. 1,3,5- Trimethoxybenzene was then added, the reaction mixture was concentrated, and the resulting residue taken up in CDCl 3 for determination of yield of 15 by 1 H-MR spectroscopy. MR data matched previously reported values. 11 S13

MR spectra 2.00 8.03 12.27 7.27 5.05 5.04 5.03 5.02 5.00 1.79 1.77 1.76 1.76 1.75 1.73 1.00 0.98 0.97-0.00 1b 189.67 184.53 88.80 77.48 77.23 76.98 27.49 9.45 1b S14

2.00 8.16 4.16 4.10 7.26 5.55 5.55 5.54 5.54 5.53 5.53 5.52 1.99 1.99 1.98 1.97 1.97 1.96 1.95 1.93 1.92 1.83 1.82 1.82 1.81 1.80 1.79 1.69 1.69 1.69 1.68 1.67 1.67 1.66 1.57-0.00 1c 1c 189.81 184.29 88.05 77.49 77.23 76.98 33.86 23.75 S15

2.00 7.97 12.11 7.26 5.72 5.71 5.69 5.68 5.67 1.86 1.85 1.84 1.84 1.83 1.82 1.81 1.81 1.81 1.80 1.80 1.02 1.01 1.00-0.00 2b 218.63 186.60 89.24 77.48 77.23 76.98 27.57 9.42 2b S16

2.00 8.14 4.19 4.09 7.26 6.08 6.07 6.06 6.06 6.05 2.04 2.03 2.03 2.02 2.02 2.01 2.01 2.00 1.87 1.87 1.86 1.85 1.85 1.84 1.73 1.72 1.71 1.70 1.70 1.70 1.69 1.68 1.55 2c 218.82 186.27 88.87 77.48 77.23 76.98 34.37 23.94 2c S17

S18 4.23 4.25 8.00 0.93 0.92-0.00 1.67 1.68 1.68 1.69 1.69 1.70 1.70 1.70 1.71 1.71 1.72 1.81 1.82 1.83 1.84 1.85 1.96 1.96 1.97 1.97 1.98 1.98 1.99 2.00 2.00 2.00 2.01 2.02 2.03 2.03 5.57 5.57 5.58 5.58 5.59 5.59 5.60 5.86 5.87 5.88 5.88 5.89 7.26 23.68 23.87 33.93 34.11 76.98 77.23 77.48 88.03 89.31 184.96 185.21 187.69 223.18 3b 3b

S19 4.00 10.24 1.30 0.22 0.23 0.10 2.49 2.51 4.67 4.69 5.14 5.15 5.21 5.23 5.31 5.32 5.44 5.45 7.31 7.32 7.33 7.34 7.34 7.35 7.36 7.37 7.38 7.39 7.40 7.40 7.42 8.80 8.81 8.82 9.38 9.39 9.40 9.68 10.05 10.06 10.07 39.01 39.18 39.34 39.51 39.68 39.85 40.00 46.00 127.74 127.95 128.74 137.39 169.96 204.08 4a 4a

2.00 2.00 21.97 8.41 8.39 4.71 4.70 4.69 1.98 1.96 1.72 1.71 1.70 1.58 1.56 1.37 1.35 1.33 1.31 1.29 1.27 1.24 1.23 1.20 4b 203.45 169.63 51.58 40.01 39.84 39.68 39.51 39.34 39.17 39.01 24.49 23.77 4b S20

2.00 18.50 8.70 2.51 2.49 1.60 4c 203.93 172.08 54.61 40.01 39.84 39.68 39.51 39.34 39.18 31.01 4c S21

1.81 10.00 10.86 7.17 7.04 4d 207.94 169.42 136.88 128.49 125.27 122.12 40.01 39.84 39.68 39.51 39.34 39.17 39.01 4d S22

S23 12.02 2.10 4.00 1.67 1.93 1.95 3.68 6.12 6.31 8.72-20 -10 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 f1 (ppm) -1000 0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000 11000 12000 MR_7_106_C_3 39.01 39.18 39.34 39.51 39.68 39.84 40.01 55.10 97.66 100.48 139.01 160.16 169.70 4e 4e

S24-1.0-0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5 11.0 f1 (ppm) -500 0 500 1000 1500 2000 2500 3000 3500 4000 4500 5000 5500 MR_7_14_2017_A 1.97 4.04 2.00 1.93 1.93 1.95 7.62 7.69 9.16 S S H H F F F F F F F F F F F F 0.89 1.06 1.22 1.39 1.55 1.72 1.89 118.37 119.98 122.89 123.24 125.05 132.82 133.09 139.87 170.28 213.26 4f 4f

S25 4.25 4.02 1.19 0.71 0.33 0.60 5.00 0.33 0.55 1.60 1.61 1.62 1.63 1.64 1.65 1.74 1.75 1.88 1.89 1.92 1.93 1.95 1.97 1.98 1.98 2.00 2.49 2.51 2.52 4.55 4.57 5.18 5.20 6.32 6.32 6.36 6.36 6.37 6.37 6.38 7.30 7.31 7.31 7.32 7.32 7.34 7.35 7.36 7.37 7.38 7.39 7.41 10.14 10.15 10.17 10.24 10.26 10.27 23.03 23.20 33.66 39.01 39.18 39.34 39.51 39.68 39.84 40.01 45.94 47.91 87.24 87.26 127.41 127.61 127.70 127.88 128.57 128.64 137.17 137.20 172.70 175.15 182.16 182.84 205.58 217.43 217.74 5a 5a

S26 4.11 8.12 2.00 2.02 0.99-0.00 0.01 1.69 1.70 1.72 1.77 1.78 1.79 1.80 1.97 1.98 1.99 1.99 2.02 2.03 2.03 2.04 2.05 2.06 2.06 3.67 3.68 3.69 3.70 4.15 4.17 4.17 4.18 6.44 6.44 6.45 6.45 6.46 7.26 23.85 24.73 25.27 34.46 49.51 50.12 76.98 77.23 77.48 87.75 171.29 182.28 205.50 218.02 5b 5b

0.98 4.14 1.15 1.00 4.03 4.26 11.59 7.41 7.40 7.28 7.27 7.26 7.25 7.24 6.47 2.51 2.49 1.66 1.65 5c 217.51 207.97 181.61 172.54 136.27 128.68 126.02 122.21 87.75 40.01 39.84 39.68 39.51 39.34 39.18 39.01 33.67 22.96 5c S27

S28-0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.5 11.5 12.5 f1 (ppm) -200 0 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000 3200 3400 MR_7_17_H 6.34 4.15 1.00 1.00 4.19 0.99 0.91 1.72 1.74 1.76 1.77 1.79 6.13 6.14 6.15 6.16 6.17 7.27 7.28 7.39 7.40 7.42 11.63 S S H O -20-10 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 f1 (ppm) -200 0 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000 3200 3400 MR_7_17_C 8.66 26.28 39.01 39.18 39.34 39.51 39.68 39.84 40.01 86.96 122.19 126.07 128.77 136.28 172.29 182.12 207.69 217.38 5d 5d

S29-0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.5 11.5 12.5 f1 (ppm) -200 0 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000 3200 3400 3600 3800 MR_7_164_H 4.19 4.05 3.03 1.00 2.04 2.03 0.98 1.64 1.66 1.93 3.76 6.46 6.95 6.96 7.32 7.34 11.49 S S H O O H 3 C -20-10 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 f1 (ppm) -200 0 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000 MR_7_164_C 22.95 33.67 39.01 39.18 39.34 39.51 39.68 39.84 40.01 55.37 87.59 113.91 123.70 129.32 157.55 172.07 181.60 206.77 217.73 5e 5e

S30 4.35 4.18 1.00 1.05 2.05 0.96 1.60 1.62 1.64 1.96 1.98 1.99 2.00 2.49 2.51 2.52 6.46 7.97 8.14 11.85 23.18 33.68 39.01 39.18 39.34 39.51 39.68 39.85 40.01 88.40 118.39 119.68 121.85 122.19 124.02 126.19 130.44 130.72 130.98 131.24 138.55 172.95 181.90 210.05 216.46 5f 5f

S31-1.0-0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5 11.0 f1 (ppm) -200 0 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000 3200 MR_7_180_H 9.12 3.12 0.90 0.99 2.02 1.95 1.00 2.03 5.47 0.73 0.77 0.81 1.39 1.39 1.41 1.44 1.53 1.53 1.55 1.58 1.61 1.68 1.71 1.74 1.76 1.80 1.89 1.93 1.95 2.44 2.46 2.90 2.92 3.21 3.25 3.37 3.39 3.41 3.47 3.49 4.90 5.28 5.29 5.31 5.32 5.38 5.39 5.40 5.42 7.31 7.32 7.34 7.38 7.39 7.40 7.44 7.45 8.53 9.20 10.04 H H S S + H Cl - -20-10 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 f1 (ppm) 0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000 MR_7_180_C 0.89 1.06 1.22 1.39 1.56 1.72 1.89 22.51 23.99 24.22 24.26 24.52 24.86 36.59 47.90 48.17 53.62 54.02 68.76 118.35 128.87 128.96 129.86 138.80 172.10 172.98 205.49 207.92 6a.HCl 6a.HCl

S32-0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.5 11.5 12.5 f1 (ppm) -100 0 100 200 300 400 500 600 700 800 900 1000 1100 1200 MR_7_176_H 3.22 2.18 3.52 1.00 1.00 2.07 2.07 0.98 1.13 2.13 0.84 0.87 0.89 1.40 1.40 1.42 1.44 1.46 1.56 1.58 1.64 1.66 1.79 1.80 1.80 1.81 1.90 1.93 1.95 2.45 2.48 2.93 2.95 2.98 3.23 3.26 3.28 3.31 3.34 3.44 3.46 3.49 3.51 4.95 7.20 7.21 7.23 7.36 7.38 7.39 7.76 7.77 8.48 9.99 11.05 S S H H + H Cl - -20-10 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 f1 (ppm) -1000 0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000 11000 12000 13000 14000 15000 16000 17000 MR_7_176_C 0.89 1.06 1.22 1.39 1.56 1.72 1.89 22.48 23.98 24.23 24.29 24.46 24.83 36.49 48.22 53.81 54.13 68.87 118.33 123.07 126.46 129.89 137.65 171.24 173.04 206.96 208.53 6b.HCl 6b.HCl

S33-0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.5 11.5 12.5 f1 (ppm) -100 0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 MR_7_178_H 3.18 2.22 3.30 0.98 1.00 2.06 2.07 3.30 0.97 2.17 1.97 0.91 0.93 0.94 1.41 1.43 1.46 1.58 1.63 1.65 1.78 1.79 1.80 1.81 1.90 1.93 1.95 2.14 2.18 2.20 2.45 2.47 2.93 2.95 3.23 3.25 3.27 3.31 3.41 3.43 3.46 3.50 3.52 3.79 4.95 6.92 6.93 7.66 7.68 8.47 9.93 10.99 S S H H - H O H 3 C Cl - -20-10 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 f1 (ppm) -1000 0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000 11000 12000 13000 MR_7_178_C 0.89 1.06 1.22 1.39 1.56 1.72 1.89 22.48 23.92 24.22 24.29 24.40 24.79 36.42 48.25 53.72 54.10 56.30 68.91 115.02 118.33 124.70 130.65 158.81 170.72 206.96 207.05 6c.HCl 6c.HCl

S34 3.27 2.27 3.39 2.27 3.53 1.02 1.07 2.16 2.09 1.00 1.00 0.80 2.15 0.91 0.83 1.40 1.42 1.44 1.45 1.68 1.79 1.80 1.81 1.81 1.90 1.92 1.93 1.93 1.95 2.14 2.17 2.19 2.95 2.97 3.27 3.34 3.36 3.45 3.47 3.49 4.93 7.75 8.33 8.42 9.98 11.60 0.84 1.01 1.17 1.34 1.51 1.67 1.84 22.43 24.00 24.22 24.24 24.44 24.76 36.38 48.39 53.95 54.12 68.91 118.32 122.54 123.26 125.42 13 132.77 133.04 139.74 171.32 172.99 206.87 210.85 6d.HCl 6d.HCl

S35 2.04 2.08 1.11 0.74 1.25 1.01 3.03 3.10 0.95 1.00 0.97 2.05 1.87 0.80 0.84 0.86 1.37 1.38 1.39 1.41 1.79 1.80 1.80 1.89 1.89 1.90 1.93 1.93 1.95 2.09 2.11 2.30 2.33 2.77 2.78 3.37 3.37 3.39 3.40 3.41 3.42 5.21 7.20 7.22 7.23 7.36 7.38 7.39 7.78 7.80 8.42 10.35 11.51 0.89 1.06 1.23 1.39 1.56 1.72 1.89 23.41 24.37 24.46 35.40 37.95 43.59 53.68 68.92 118.35 123.25 126.56 129.93 137.59 171.25 173.43 206.78 208.30 6e.HCl 6e.HCl

S36 2.07 2.07 1.00 0.73 0.91 1.00 3.14 3.08 0.98 1.00 1.02 0.95 2.03 0.90 0.91 1.39 1.42 1.45 1.55 1.55 1.57 1.63 1.65 1.81 1.90 1.93 1.95 2.12 2.13 2.15 2.16 2.30 2.78 2.91 3.42 3.43 3.45 3.45 3.47 3.48 5.13 5.14 5.15 5.16 5.17 5.18 5.19 5.20 7.74 8.25 8.47 10.32 12.06 0.89 1.06 1.23 1.39 1.56 1.72 1.89 23.45 24.37 24.40 35.45 38.08 43.60 53.90 69.10 118.36 122.58 123.30 125.47 132.31 132.58 132.84 139.86 171.43 173.46 206.72 210.80 6f.HCl 6f.HCl

S37 1.32 2.31 3.10 1.16 2.15 1.09 4.07 0.70 2.07 1.00 0.68 1.97 1.02 1.56 0.85 0.82 0.80 0.59 0.61 0.59 1.14 1.76 1.93 1.95 2.00 2.81 3.34 3.69 3.71 4.15 4.38 5.11 5.13 5.16 5.20 5.85 7.53 7.70 7.72 8.06 8.37 8.54 8.56 8.61 9.03 9.82 11.72 12.36 0.89 1.06 1.23 1.39 1.56 1.72 1.89 24.08 24.57 27.48 37.43 43.67 54.95 58.05 103.93 117.79 118.32 119.32 121.01 122.92 123.17 125.26 125.34 129.89 132.28 132.55 132.82 136.56 138.47 138.67 139.37 141.85 161.95 168.86 171.55 207.19 209.64 6g.2HCl 6g.2HCl

S38 3.36 4.79 4.05 4.09 0.57 0.42 0.54 0.40 1.34 1.38 1.61 1.63 1.65 1.67 1.69 1.71 1.73 1.74 1.74 1.75 1.87 1.88 1.88 1.89 1.90 1.92 1.96 1.97 2.49 2.51 6.31 6.31 6.32 6.32 6.33 6.33 6.49 6.50 6.50 6.51 6.51 9.43 9.74 23.06 23.30 29.47 29.74 33.66 33.68 39.01 39.18 39.34 39.51 39.68 39.84 40.01 53.83 54.44 85.74 175.55 176.84 178.54 180.99 183.16 184.57 203.38 205.50 7 7

S39 4.18 9.30 1.10 1.04 1.06 1.08 6.11 1.18 1.00 1.01 0.99 1.13 1.16 1.18 1.21 1.25 1.42 1.62 1.73 1.82 1.85 2.07 2.09 2.20 2.33 2.34 2.35 2.36 2.37 2.51 4.79 4.81 4.83 4.85 7.82 7.84 8.46 21.10 24.06 24.25 29.91 35.90 39.01 39.18 39.34 39.51 39.68 39.82 40.01 53.00 53.66 65.84 169.23 171.84 178.53 200.12 8 8

HPLC traces The catalyst used is shown above the trace. Racemic: O O H 12a H S S H 6e H S40

F 3 C O O F 3 C H H 12b F 3 C S S F 3 C H H 6f F 3 C O O F 3 C H H 12c S41

F 3 C S S F 3 C H H 6d O O Ph H H 12d S S Ph H H 6a S42

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