DiPel Biological Insecticide Horticulture Crops Technical Manual Innovative solutions. Business made easy.
Aim: Getting safe, reliable, cost-effective control of Lepidoptera pests in a full spectrum of horticulture crops (including conventional and organic production).
Introduction: DiPel is the number one biological insecticide in the world. This potent Bacillus thuringiensis (Bt) insecticide provides proven control of Lepidoptera pests in many crops. Its balanced blend of four active toxins, plus a natural spore, provides exceptional control. This varied list of active toxins also makes DiPel an effective resistance management tool. DiPel can be applied in conventional or organic systems. Because it is Exempt from Tolerance, DiPel gives grower s full confidence when selling crops to foreign markets. In addition, DiPel s superior, high-potency, dry-flowable formulation ensures the longest shelf life of any other Bt on the market. Benefits of Using Diepel: It deters the worm from feeding immediately, protecting the crop from damage. Death occurs within 1 to 3 days. DiPel s dry-flowable formulation is convenient and easy to handle. DiPel s unique mode of action is an excellent tool for managing resistance of many pests. It can be readily tankmixed to improve the performance of traditional insecticides or used as a rotation partner in conjunction with a traditional insecticide program. Using DiPel can increase the overall value of your insect control program. Growers can enjoy improved performance at equal or less costs. DiPel is not harmful to bees or beneficial insects making it an excellent tool for insect management at bloom time as well as in an intergraded pest management programs (IPM). No re-entry interval restriction, allowing quick access to your crops. Exempt from maximum residue limits in Canada so you can apply up to harvest. Residual last between 7 and 10 days depending on formulation. Rain fastness is 3 hours. Safe to humans and crops. 3
Other Benefits of using DiPel: The safety and environmental profile of DiPel insectide results in no re-entry intervals and the shortest pre-harvest intervals (PHI) allowed by law. This gives growers great flexibility throughout the growing season. In addition, DiPel is exempt from tolerance, which means growers don t need to worry about selling into export markets due to residue issues. DiPel is OMRI-certified for use in organic production. It provides organic growers with a proven and highly effective natural tool to fight insect pests.
Mode of Action: DiPel is created with a naturally occurring bacterium found in soil and on plant leaves throughout most regions of the world. It contains insecticidal proteins and spores that become active inside the insect s gut when ingested by Lepidopteran larvae. The most potent Bt products deliver multiple modes of action in the insect s gut, which make it a valuable tool in resistance management. In fact, many new insecticide technologies are being met with resistance emphasizing the need for Bt insecticides. Being the strongest Bt product on the market DiPel goes to work quickly. Once ingested, the Bt toxins bind to the midgut cells, creating performances in the gut lining and causing the insect to stop feeding within several minutes. Bt spores then penetrate the body cavity and circulatory systems. The spores germinate, spread through the body and cause poisoning. Insects start to die one to three days after ingestion. While lethal to Lepidopteran pests, studies have shown that DiPel does not harm humans, the environment or beneficial insects that are pests natural predators. Caterpillar pest ingests Bt product (toxins and spores), and the pest stops feeding within several minutes. The crop is protected. 1. The Bt toxin crystals dissolve in the midgut. 2. The Bt toxins are activated and attach to midgut receptors. 3. Pores form in the cell wall and cells rupture. 4. Bt spores will invade the insect body, death follows. 5
Formulations: DiPel is available in two convenient formulations: DiPel 2X DF and DiPel WP. Registered Crops and Rates for DiPel 2X DF: Apples: 1125 1 6 7 5 g / h a ( 4 5 5 6 7 8 g / a c ) Blueberry (Lowbush): 550 1125 g/ha (223 455 g/a c) Blueberry (Highbush): 1680 g/ha (680 g/ac) Broccoli: 55 550 g/ha (22 223 g/ac) Cabbage: 55 550 g/ha (22 223 g/ac) Cauliflower: 55 550 g/ha (22 223 g/ac) Chinese Cabbage: 55 550 g/ha (22 223 g/ac) Bok Choy: 55 550 g/ha (22 223 g/ac) Chinese Broccoli: 55 550 g/ha (22 223 g/ac) Asian Radish: 55 550 g/ha (22 223 g/ac) Chokeberry: 635 g/ha (257 g/ac) Collards: 275 550 g/ha (111 223 g/ac) Cranberry: 275 550 g/ha (111 223 g/ac) Ginseng: 565 1125 g/ha (229 455 g/ac) Grape: 1125 g/ha (455 g/ac) Herbs and Spices: 275 550 (111 223 g/ac) Kale: 275 550 g/ha (111 223 g/ac) Lettuce: 275 550 g/ha (111 223 g/ac) Mustard Greens: 275 550 g/ha (111 223 g/ac) Nut crops, Parsley: 1125 1675 g/ha (455 678 g/ac) Pears: 1125 1675 g/ha (455 678 g/ac) 6
Potatoes: Raspberry: Spinach: 560 1120 g/ha (227 453 g/ac) 550 1125 g/ha (223 455 g/ac) 275 550 g/ha (111 223 g/ac) Stone Fruit (Apricot, Cherry, Plum): 1125 1 6 7 5 g / h a ( 4 5 5 2 2 3 g / a c ) Sweet and Field Corn: Timothy: Turnip Greens: Tobacco: Tomato: Berries and small Fruit (Crop Group C13 07): Sunflower: Greenhouse Ornamentals: Greenhouse Herbs: Greenhouse Vegetables (pepper, tomato, cucumber): 560 1120 g/ha (227 453 g/ac) 140 275 g/ha (57 111 g/ac) 275 550 g/ha (111 223 g/ac) 55 140 g/ha (23 57 g/ac) 140 550 g/ha (57 223 g/ac) 225 1125 g/ha (91 455 g/ac) 315 625 g/ha (127 253 g/ac) 625g/1000L 625g/1000L 625g/1000L Ornamental and Shade trees: 65 250g /400L Sea Buckthorn: 125 250 g/400l Registered Pests DiPel 2X DF: Leafrollers (Fruitree, European, Oblique banded, Three lined), Winter moth, Blueberry spanworm, Chainspotted geometer, Rannoch looper, Cabbage looper, Diamondback moth, Imported cabbageworm, Fall Webworm, Green and Brown spanworm, Grape Berry moth, Cranberry Fruitworm, Cherry Fruitworm, Sunflower moth, European Corn Borer, Essex (European) skipper, Hornworm, Duponchelia foveali, Tent caterpillar, Elm spanworm, Fall webworms, Spring and Fall cankerworm, Bagworm, Gypsy moth, Spruce budworm. 7
Registered Crops and Rates for DiPel WP: Apples: Broccoli: Cauliflower: Collards: Cranberries: Kale: Lettuce: Mustard Greens: Parsley: Pears: Raspberry: Spinach: Stone Fruit (Peachy, Cherry, Plum, Apricot): Sunflower: Timothy: Turnip Green: Tobacco: Tomatoes: Ornamental and Shade Trees: 225 3350 g/ha (910 1356 g/ac) 110 1100 g/ha (45 445 g/ac) 110 1100 g/ha (45 445 g/ac) 550 1100 g/ha (45 445 g/ac) 550 1100 g/ha (223 445 g/ac) 550 1100 g/ha (223 445 g/ac) 550 1100 g/ha (223 445 g/ac) 550 1100 g/ha (223 445 g/ac) 550 g/ha (223 g/ac) 2250 3350 g/ha (910 1356 g/ac) 1100 2250 g/ha (445 910 g/ac) 550 1100 g/ha (223 445 g/ac) 2250 3350 g/ha (910 1356 g/ac) 625 1120 g/ha (253 453 g/ac) 275 550 g/ha (111 223 g/ac) 550 1100 g/ha (223 445 g/ac) 110 275 g/ha (45 223 g/ac) 275 1100 g/ha (223 445 g/ac) 250 500 g/400l 8
Registered Pests DiPel WP: Leafrollers (Fruitree, European, Oblique-banded, Three-lined), Winter moth, Cabbage looper. Diamondback moth, Imported cabbageworm, Fruitworm, Sunflower moth, Essex (European) skipper, Hornworm, Tomato fruitworm, Tomato hornworm, Tent caterpillar, Elm spanworm, Fall webworms, Spring and Fall cankerworm, Bagworm, Gypsy moth. Application Rate and Timing: Apply DiPel when insects pests are small (early instars) before crop damage has occurred. DiPel should be applied to actively growing larvae. The pest will stop feeding immediately and die within 1-3 days. Repeat at an interval sufficient to maintain control, usually 3-14 days depending on plant growth rate and population density, rainfall after treating and other factors. The best application timing is at egg hatch of the target pests, from petal fall through to green fruit stage. Weekly applications may be necessary if egg hatch is asynchronous. Also, DiPel application is similar to all common insecticides: best applied in the evening or in overcast conditions. Good coverage is essential to ensure the crop is well protected. Apply in sufficient water volume to cover all foliage and fruit surfaces. A spreader-stick which has been approved for use on growing and harvesting crops should be added to hard to wet crops such as cabbage, or to improve weather fastness of the spray deposits. The best time to apply is when the larvae are young. After eating a lethal dose of DiPel, the pest will stop feeding immediately and die within 1-3 days. Mortality varies with with larval size, lepidopteran species and dose consumed. In heavy pest pressure, higher rates of DiPel are recommended. 9
The Program: Early season: Apply DiPel alone at bloom or add it to a tank-mix to improve insect control. You can also use DiPel as an economical alternative to other soft insecticides to preserve beneficial insects and to avoid flaring secondary insects. Mid-Season: Maximize during the middle. Use DiPel mid-season to improve performance and reduce the risk of resistance due to DiPel s multiple modes of actions. Late Season: Finish with flexibility. Use DiPel late season and enjoy the shortest re-entry and pre-harvest intervals allowed by law. With no re-entry intervals restrictions and shortest pre-harvest intervals allowed by law, DiPel can be used late season. DiPel treated cabbage 14 days after application. Untreated cabbage.
Resistance Management: DiPel 2X DF has a unique mode of action, not related to chemical insecticides. DiPel 2X DF is an IRAC class 11 insecticide, no cross resistance has ever been observed with synthetic insecticides. DiPel 2X DF contains four separate insecticidal Bt toxins making it difficult for resistance to develop. Quality: DiPel is produced using the latest technology available. From the fermentor to the field, DiPel production is overseen by using strict quality standards. Every lot of DiPel has been tested in the lab on thousands of insects before commercial release into the market. Growers benefit from the same quality control and superior manufacturing expertise that goes into human health products. Each gram of DiPel offers consistent quality which translates to consistent field performance leading to profitable pest control in crop production. 11
Mixing Instructions: Partially fill spray tank with water and provide moderate agitation. Add the required amount of DiPel Biological Insecticide while maintaining agitation. Add remaining water, if any. Maintain moderate agitation while loading and spraying. Do not mix more DiPel Biological Insecticide than can be used in a 12- hour period. Compatibility: DiPel can be tank-mixed with most other other insecticides and fungicides as well as Plant Growth Regulators. Storage: In order to ensure microbial purity and potency DiPel Biological Insecticide should be stored in the original container at 0 to 25 C and use within 24 months of the date of manufacture. With no re-entry intervals restrictions and shortest pre-harvest intervals allowed by law, DiPel can be used late season. Quality in Formulation: DiPel provides excellent wetting properties and easy suspension. (2X DF formulation pictured.)
Comparing DiPel to Conventional Insecticides: Resistance Mode of Action Mode of Action Feeding cessation Residual, MRL limitations Safety to other arthropods Restrictions Organic status Labeled Crops DiPel Unique, no cross-resistance with any chemical insecticide. Unique mixture of toxins. Disruption of gut cells. Fast, measured in minutes. None, PHI either 0 or minimal. Safe for beneficial predators, parasites and bees. Few restrictions on timing or location. Yes, OMRI, NOP, others in Europe, Asia and Latin America. Globally over 300. Conventional Insecticides Possible cross-resistance. Single active. Varied mode of action. Good-Excellent. PHI enforced due to MRL. Often toxic to beneficial insects. Generally, some toxicity to non-target organisms. Rare. Variable. 13
Why Use DiPel? To reduce chemical insecticide residue on food crops. Due to limits on amounts and frequency of chemical insecticide use. No worker re-entry interval restriction. For chemical insecticide resistance management (IPM). To provide the best possible control conditions for beneficial insects such as honey bees. As activator to enhance efficacy of chemical insecticide. As one of the few insecticides approved for organic use (OMRI listed). MRL for export tolerance. For environmental reasons. Return on Investment (ROI). DiPel OBLR Control in Gala Apples 14 days after application. Mattawa, WA. Application dates 4/24, 5/2. Airblast sprayer 100 gal/ac. 14
Notes: 15
Nufarm is the marketer of Valent products in Canada. To learn more about DiPel or any other Nufarm products visit our website or call our technical support line, toll-free. www.nufarm.ca 1-800-868-5444 This information is for marketing purposes only. ALWAYS READ AND FOLLOW THE LABEL BEFORE USING ANY NUFARM PRODUCTS. DiPel is a registered trademark of Valent BioSciences Corporation. January 2013