Applications of In Situ Hybridisation in Research and Disease

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Cancer Research UK Cambridge Institute Applications of In Situ Hybridisation in Research and Disease 7 8 May 2015

Programme Applications of In Situ Hybridisation in Research and Disease 11

Thursday 7 May 9.00 9.05 Welcome from Will Howat Session 1 Chromogenic and Fluorescent In Situ Hybridisation J Howard Pringle, University of Leicester 9.05 9.10 Introduction of the Chair by Will Howat 9.10 9.40 J Howard Pringle University of Leicester Probe technology for in situ hybridisation 9.40 10.10 Sheila O Connor St James s Institute of Oncology, Leeds Detection of BCL2 gene rearrangements in the Reed-Sternberg cells of composite lymphoma 10.10 10.40 Jane Starczynski Heartlands Hospital, Birmingham Tumours break rules: The importance of integrating pathology and genetics in identifying patients for targeted therapy. 10.40 11.10 Coffee/tea 11.10 11.40 Thomas Bjarnsholt University of Copenhagen The in vivo Biofilm 11.40 12.10 Aldo Ciau-Uitz University of Oxford Gene expression dynamics indicate the establishment of gene regulatory networks during haematopoietic stem cell programming 12.10 12.25 Selected talk from submitted abstracts : José Luis Ferran University of Murcia, Spain Exploring Brain Genoarchitecture by Chromogenic In Situ Hybridization in Whole-Mount, Cryostat or Floating sections 12.25 12.55 Panel discussion introduced by J Howard Pringle, University of Leicester 12.55 14.30 Lunch and poster session 12 CRUK CI In Situ Hybridisation Symposium 2015

Session 2 Automation of In Situ Hybridisation Julia Jones, Cancer Research UK Cambridge Institute 14.30 14.35 Introduction of the Chair by Will Howat 14.35 15.05 Julia Jones Cancer Research UK Cambridge Institute Multiplex ISH on the Bond Rx 15.05 15.35 Paul Murdock Asterand Bioscience, Royston Experiences with the latest generation in situ hybridisation methodologies 15.35 16.05 Adrian Murillo Ventana Medical System, Tucson Automated ISH as a System 16.05 16.30 Coffee/tea 16.30 17.00 Amy Bernard Allen Institute for Brain Science, Seattle Automating Atlases: Development of an in situ hybridization platform for integrating data acquisition and analysis 17.00 17.30 Stephen Taylor University of Oxford Zegami: A tool for image data exploration and analysis 17.30 17.45 Selected talk from submitted abstracts: Vikram Deshpande Massachusetts General Hospital Application of RNA in situ Hybridization in Routine Clinical Practice: targeting Albumin, hr-hpv and Kappa/Lambda 17.45 18.15 Panel discussion introduced by Julia Jones, Cancer Research UK Cambridge Institute 18.15 Drinks reception 18.45 Buffet dinner at the CRUK Cambridge Institute Applications of In Situ Hybridisation in Research and Disease 13

Friday 8 May 8.30 9.00 Coffee/tea Session 3 Single Molecule Detection In Situ Hybridisation Scott Fraser, University of Southern California 9.00 9.05 Introduction of the Chair by Will Howat 9.05 9.35 Scott Fraser University of Southern California Multiplex Imaging and Sensing the Signatures of Embodied Cells 9.35 10.05 Sanjay Tyagi Public Health Research Institute Center, New Jersey Studying barriers to transmission of transcriptional noise by single molecule-fish 10.05 10.35 Shalev Itzkovitz Weizmann Institute of Science, Israel Bursty gene expression in the intact mammalian liver 10.35 11.05 Coffee/tea 11.05 11.35 Ola Söderberg Uppsala University, Sweden Molecular tools for single-cell analysis: use of the Proximity Ligation Assay 11.35 12.05 Je H. Lee Cold Spring Harbor Laboratory Next Generation Sequencing In Situ: Implications for biomarker discovery and cancer biology 12.05 12.35 Panel discussion introduced by Scott Fraser, University of Southern California 12.35 13.30 Lunch 14 CRUK CI In Situ Hybridisation Symposium 2015

Session 4 Non-coding RNA In Situ Hybridisation Boye Schnack Nielsen, Bioneer, Denmark 13.30 13.35 Introduction of the Chair by Will Howat 13.35 14.05 Boye Schnack Nielsen Bioneer, Denmark Visualizing the world of non-coding RNA 14.05 14.35 Yuling Luo Advanced Cell Diagnostics Inc, California The Renaissance of In Situ Hybridization 14.35 15.05 John Le Quesne University of Leicester Semi-quantitative LNA-ISH for the detection of micro-rna expression in tissue microarrays of archival breast tumour tissue 15.05 15.35 Coffee/tea 15.35 16.05 Lorenzo Sempere Van Andel Institute, Michigan Tissue slide based multiplex assays for co-detection of microrna and protein biomarkers in translational cancer research 16.05 16.35 Thomas Tuschl The Rockefeller University, New York Characterization of mirna expression patterns by small RNA sequencing and mirna-fish in archival tissues 16.35 16.50 Selected talk from submitted abstracts: Joseph Krueger Flagship Biosciences Automated Quantitative Chromagenic In Situ Hybridization to Enable Companion Diagnostic Approaches 16.50 17.20 Panel discussion introduced by Boye Schnack Nielsen Bioneer, Denmark 17.20 17.30 Announcement of poster prize winner and symposium close Applications of In Situ Hybridisation in Research and Disease 15