Stream Observation Data Sheet School: Charleroi Middle School Date 9/24/15 Stream Study Site: Maple Creek. Macroinvertebrate Survey

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Stream Observation Data Sheet School: Charleroi Middle School Date 9/2/15 Stream Study Site: Maple Creek Teacher: Mr. Johnson Latitude_210 7 degrees NORTH Yesterday Group Members: Period 7 th Longitude_79 5 18 degrees WEST Weather Today Air Temperature 77 C or F Cloud Cover clear partly cloudy cloudy Air Temperature 6 C or F Cloud Cover clear_ partly cloudy cloudy Precipitation 0.0 in Precipitation 0.0 in How could yesterday s weather affect today s field study? Lack of rain can affect the level of the stream Macroinvertebrate Survey Collection method used: Kick-Seine or D-Net (circle). If using a kick-seine, collect samples times. If using a D-net, collect 20 scoops and record the number of scoops taken from each of the habitat areas in the table Check all of the macroinvertebrates that you find in your stream and calculate the stream s water quality rating [you may also record the number of each captured, but to calculate the rating at the bottom, only count each kind of animal once, regardless of the quantity found]. Benthic Habitat Sampled Habitat # scoops Riffle 111 Rootwads/ woody 11 debris/ leaf pack Submerged 1 Undercut s Other (specify): TOTAL 20 SENSITIVE LESS SENSITIVE TOLERANT to pollution of pollution Caddisflies (except net spinners) Caddisflies, common net spinning Crayfish Aquatic worms Mayflies Dobsonflies Scuds 1 Black flies Stoneflies Fishflies Aquatic sowbugs Midge flies 1 Watersnipe flies 1 Crane flies Clams Leeches 5 Riffle beetles Damselflies Mussels Lunged snails 10 Water pennies Dragonflies Salamanders 1 Worm 2 Gilled snails Alderflies 1 Caterpillar # of check marks (# of kinds found) # of check marks (# of kinds found) count both columns under less sensitive # of check marks (# of kinds found) 18 5 Now add up the numbers you calculated for all three categories, above, and write the total # here:89 # above x = 5_ # above x 2 = 20 5 # above x = 15 WATER QUALITY RATING Circle the rating that corresponds to the total of your columns. Excellent: > 22 Good: 17-22 Fair: 11 16 Poor: < 11

Stream Habitat Assessment Apparent Color of Water Brown Stream speed Slow Odor of Water None Water Temperature 57º Characteristics for stream habitat assessment Characteristic Verge % Bare Soil On Erosion Slumping and movement Bends and Riffles Turbidity (JTU s) Aquatic Sediment Deposition Excellent Score present, Canopy intact Good Score and canopy nearly intact Fair Score 2 Disturbed Poor Score 1 Cleared land or urban development Site Score in slightly severely undisturbed state disturbed moderately disturbed disturbed 0-10% 11-0% 1-80% 81-100% Stable, no sign of erosion No movement Very occasional local erosion Slight movement on the bank Some erosion evident Moderate bank collapses Severe bank failure; extensive cracking and fall-ins Severe bank failure; extensive cracking & fall-ins Bends present, 5-10 Bends present, 1- Occasional bend, 1-2 Straight channel, riffles in 10 meters, riffles in 10 meters, riffles in 50 meters, riffles/pools absent, no many snags some snags few snags snags 0-10 11-0 1-150 >150 Little vegetation - uncluttered look; fairly small numbers of many different kinds of plants Less than 20% of stream bottom affected by deposition; minor accumulation of fine and coarse material at snags and little or no enlargement of islands or point bars Moderate amounts of vegetation 20-50% of stream deposition; moderate accumulation; substantial sediment movement during major storms; some new increase in bar formation Cluttered, weedy conditions; vegetation sometimes luxurious and green; seasonal algal blooms 50-80% of stream deposition; pools shallow, heavily silted; embankments may be present on both banks; frequent and substantial sediment movement during storm events Choked, weedy conditions, heavy algal blooms or no vegetation at all >80% of stream deposition; heavy deposits; mud, silt, and/or sand in pools almost absent due to deposition Stream Habitat Rating 2-6 = Excellent (Natural or virtually natural state) 2-1 = Good (Some alteration from natural state) 1-22 = Fair (Significant alteration from natural state) 0-1 = Poor (Very degraded habitat) Total score 1 What rating did you give to your stream habitat? Good

Water Quality Testing (1) Follow instructions provided with each test kit to test different parameters. (2) Record your data here: DATA Dissolved Oxygen (DO) Dissolved Oxygen (DO) % Saturation See conversion chart ph Phosphate Nitrate Chloride Transparency (cm) Turbidity (JTU ~= NTU) Conductivity ( s/cm) Total Dissolved Solids (TDS) (ppm = mg/l) Water Temperature ( o C) Trial 1 100 8.0.1 15 180 1 Trial 2 100 8.25.2 15 18 1 Trial.1 191 () Circle the corresponding value here: Dissolved Oxygen (DO) % Saturation (see conversion chart) ph (units) Water Quality Summation for Chemical Tests ECELLENT GOOD FAIR POOR 80 120 70 80 120 10 7.0 7.5 6.5 7.0 7.5 8.5 50 70 > 10 5.5 6.5 8.5 9.0 < 50 < 5.5 > 9.0 Reactive Phosphate (PO ) 0 0.2 0.2 0.5 0.5 2.0 > 2.0 Nitrate (NO ) 0 5 5 10 > 10 Chloride (Cl) 0 20 20 50 50 250 > 250 Transparency (cm) > 65.0 65.0 5.0 5.0 15.5 < 15.5 Turbidity (JTU ~= NTU) 0 10 10 20 20 0 > 0 Total Dissolved Solids (ppm = mg/l) 0 150 150 250 250 50 > 50 Conductivity ( s/cm) 0 171 172 27 28 500 > 500 Based on your tests and observations, how would you rate water quality overall (e.g., if you had some excellent, some fair, mostly good, you might give an overall rating of good)? Circle your answer: Overall Water Quality Rating: Excellent Good Fair Poor [continued, next page]

Overall Stream Health Assessment Write your ratings from all three of the above tests, here: Based on your tests and observations, how would you rate the health of your stream overall? Macroinvertebrate Survey Habitat Assessment Water Quality Tests Overall Stream Health Excellent Good Fair Poor