DNA & RNA from Environmental Sources

DNA & RNA from Environmental Sources

Ultra-Pure DNA & RNA from Environmental Sources Environmental samples (soil, sludge, sediment, feces, etc.) are rich sources of novel, uncharacterized microbes that are often unable to be cultured. This microbial diversity is typically difficult to study and hindered by the lack of rapid and robust purification schemes that enable analysis. Similarly, samples refractory to conventional lysis procedures (such as plants, seeds, food, arthropods, yeast, filamentous fungi, unicellular and filamentous algae, and protozoa) require efficient methods for the isolation of DNA and RNA. Canonical procedures for DNA and RNA isolation from these samples are often lengthy, messy, inconsistent and cost prohibitive. In addition, the purity of the DNA or RNA is not always sufficient for downstream applications in molecular biology. To address these issues, Zymo Research has developed a range of technologies to easily and effectively extract DNA and RNA from these tough-to-lyse, difficult samples. The environmental DNA and RNA kits from Zymo Research feature chemically inert, high density BashingBeads to thoroughly lyse samples and liberate nucleic acids for purification. This is coupled with unique Fast-Spin column and plate technologies to maximize yield and allow for minimal volume elution in minutes. Polyphenolic compounds in environmental and plant samples (i.e., humic/fulvic acids, tannins, etc.) can confound downstream analyses such as PCR. Our proprietary resins in select products remove these compounds from the eluted DNA and RNA. This technology is also available as a stand-alone product for the cleanup of impure preparations as the OneStep PCR Inhibitor Removal Kit. At Zymo Research, we are committed to quality and guarantee that all of our products meet and exceed your expectations. USA: (888) 882-9682 INTL: (949) 679-1190 Fax: (949) 266-9452 Find us on Facebook & follow us on Twitter for the latest product releases, special offers, and more! Your questions, comments, and suggestions are always welcome info@zymoresearch.com Sign up to receive our newest publications http://www.zymoresearch.com/publications Please recycle this newsletter 2

BashingBead Lysis for Environmental DNA Purification The BashingBead DNA purification kits from Zymo Research are for rapid recovery of inhibitor-free PCR-ready DNA from a broad range of tough-to-lyse organisms and environmental samples. Kits have been specifically designed for the efficient recovery of inhibitor-free DNA from plants, seeds, tissues, insects, and microorganisms that inhabit soil, sludge, sediment, and fecal samples. Products are available in spin column Micro- (5 µg/prep), - (25 µg/ prep), - (125 µg/prep) and 96-well (5 µg/well) formats these formats are diagrammed below. For processing, samples are simply transferred into provided ZR BashingBead Lysis Tubes and efficiently lysed by bead beating in uniquely designed lysis buffers. Processing the samples can be performed using any bead mill, pulverizer, or vortex that can accommodate standard 2.0 ml, ml tubes, or 96-well blocks depending on the format of the kit. Following lysis, DNA is isolated using innovative Fast-Spin column and plate technologies, and in cases where plant, feces, or soil samples are processed, the DNA is subsequently filtered to remove humic/fulvic acids or polyphenols that can inhibit PCR. The isolation of inhibitor-free DNA typically takes about 15 minutes. Micro & Formats Format 96-Well Format ((( ))) PCR-Ready Ultra-pure DNA PCR-Ready, Ultra-pure DNA Pulverize Sample with ZR BashingBead Lysis Tube Filter Lysate with Zymo-Spin IV Continue with the following step for ZR Soil Microbe DNA, ZR Fecal DNA, and ZR Plant/Seed DNA Kits only. Bind, Wash, Elute DNA with Zymo-Spin IC or IIC Polyphenolic Removal with Zymo-Spin IV-μHRC or IV-HRC ((( ))) OR Spin Wash Elute PCR-Ready Ultra-pure DNA Continue with the following step for ZR Soil Microbe DNA, ZR Fecal DNA, and ZR Plant/Seed DNA Kits only. PCR-Ready, Ultra-pure DNA Pulverize Sample in ZR BashingBead Lysis/Filtration Tube Centrifuge & Transfer Lysate to Zymo-Spin V-E w/ Zymo- Filter Polyphenolic Removal with Zymo-Spin IV-HRC ((( ))) PCR-Ready Ultra-pure DNA Continue with the following steps for ZR Soil Microbe DNA, ZR Fecal DNA, and ZR Plant/Seed DNA Kits only. PCR-Ready, Ultra-pure DNA Pulverize Samples with ZR BashingBead Lysis Rack Deep-well Block Bind, Wash, Elute DNA with Silicon-A Plate Polyphenolic Removal with Silicon-A -HRC Plate www.zymoresearch.com 3

= Spin Column Application Guide: Environmental DNA Purification ZR Soil Microbe DNA Kits ZR Fungal/Bacterial DNA Kits ZR Fecal DNA Kits ZR Tissue & Insect DNA Kits ZR Plant/Seed DNA Kit = 96-Well Micro 96-Well Micro 96-Well Micro 96-Well Micro 96-Well Micro 96-Well Specifications ZR BashingBead Lysis P P P P P P P P P P P P P P P P P P P P Format DNA Binding Capacity 5 µg 25 µg. 125 µg. 5 µg 5 µg. 25 µg. 125 µg. 5 µg 5 µg. 25 µg 125 µg. 5 µg 5 µg 25 µg 125 µg 5 µg 5 µg 25 µg 125 µg 5 µg Elution Volume 10µl 25µl 1µl 25µl 10µl 25µl 1µl 25µl 10µl 25µl 1 µl 25µl 10µl 25µl 1µl 25µl 10µl 25µl 1µl 25 µl Removal of PCR Inhibitors P P P P P P P P P P P P P P P P P P P P Removal of Humic, Fulvic, Polyphenolic Substances P P P P P P P P P P P P Processing Time 15 min. 15 min. 25 min. min. 10 min. 10 min. 20 min. 40 min. 15 min. 15 min. 25 min. min. 10 min. 10 min. 20 min. 40 min. 15 min. 15 min. 25 min. min. Application Environmental Sources Soil P P P P Sediment P P P P Sludge P P P P Feces P P P P Microorganisms Bacteria P P P P P P P P P P P P Gram (+) P P P P P P P P P P P P Gram (-) P P P P P P P P P P P P Fungi P P P P P P P P P P P P Unicellular (Yeast) P P P P P P P P P P P P Filamentous P P P P P P P P P P P P Algae P P P P P P P P P P P P Unicellular P P P P P P P P P P P P Filamentous P P P P P P P P P P P P Protists P P P P P P P P P P P P Tough-to-Lyse Tissues, Organisms, Insects, & Plants Soft Tissues Some Some Some Some Some Some Some Some Some Some Some Some P P P P Solid Tissues (Food) P P P P Tough-to-Lyse Tissues P P P P Tough-to-Lyse Organisms P P P P Insects/Arthropods P P P P Plant Material P P P P Seeds P P P P Fruit P P P P

ZR Soil Microbe DNA Kits Simple, efficient isolation of humic-free DNA from microbes in soil, sludge, sediment, and sand in minutes. Ideal for tough-to-lyse bacteria, fungi, algae, and protozoa. Fast-Spin column and unique filtration technologies effectively remove PCR inhibitors from the DNA product. Samples A 1 kb B 1 3 2 4 5 P Soil Sample 2 Sample 1 Class: Sandy Clay Loam Class: Sand <5% Organic <5% Organic <% Silt/Clay <10% Silt/Clay P Sediment P Sludge Sample 3 Class: Sandy Loam <5% Organic <40% Silt/Clay Slightly Hydrophobic Sample 4 Class: Sandy Loam 7-8% Organic <25-30% Silt/Clay Coarse Sand Sample 5 Class: Sandy Loam 5-6% Organic <25% Silt/Clay Fine Gravel C D P Gram (+) Bacteria The ZR Soil Microbe Kit can be used to isolate high quality DNA from a variety of soil types which yields robust products following PCR. Panel A: Physical characteristics of sampled soils (1-5). Panel B: Microbial, genomic DNA was isolated from soil samples (1-5) using the ZR Soil Microbe Kit. Approximately 10% of the eluted DNA was then separated in a 0.8% (w/v) agarose/ethidium bromide gel. Panels C and D show the results of PCR of microbial DNA isolated from the samples with primers specific for prokaryotic 16S rrna (C) or eukaryotic rrna (D). In the figures, the 1 kb size marker (NEB) is as indicated and the arrows show the prokaryotic 16S rrna and eukaryotic rrna PCR products. P Gram (-) Bacteria P Yeast P Filamentous Fungi P Unicellular Algae P Filamentous Algae Comparative Overview P Protist Cat # Desc. s D6003 Micro D6001 D6101 25 D6002 96-Well 2 x 96 Processing Time Soil Input DNA Recovery DNA Integrity Fidelity of PCR Use of Proteases Use of Organic Denaturants ZR Soil DNA 10-15 minutes 0.25 grams Good Supplier M 40- minutes 0.25 grams Fair Supplier E 1 hour 30 minutes 0.1 grams Low Poor Low Yes ZR Fungal/Bacterial DNA Kits light Simple, efficient isolation of DNA from all types of tough-to-lyse fungi and bacteria in minutes. Zymo Research 1 kb Yeast Spores E. coli Supplier M Yeast Spores E. coli P Gram (+) Bacteria P Gram (-) Bacteria P Yeast P Filamentous Fungi Fungal and bacterial DNA purification. DNA isolated from Saccharomyces cerevisiae (spores) and E. coli using the ZR Fungal/ Bacterial DNA is high quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the ZR Fungal/Bacterial DNA or the kit from supplier M. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research). P Unicellular Algae P Filamentous Algae P Protist Comparative Overview Cat # Desc. s D6007 Micro D6005 D6105 25 D6006 96-Well 2 x 96 Processing Time DNA Recovery DNA Integrity Fidelity of PCR Use of Proteases ZR Fungal/Bacterial DNA 10-15 minutes Good www.zymoresearch.com Supplier M Supplier Q 40- minutes Fair Fair > 3 hours Fair Fair Fair Yes 5

ZR Fecal DNA Kits Rapid methods for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes including those from humans, birds, rats, mice, cattle, etc. Fast-Spin column and unique filtration technologies effectively remove PCR inhibitors from the DNA product. P Feces P Bacteria Gram (+) P Bacteria Gram (-) P Yeast P Filamentous Fungi P Unicellular Algae P Filamentous Algae P Protist Cat # Desc. s D6012 Micro D6010 D6110 25 D6011 96-Well 2 x 96 Zymo Other Suppliers Research M Q Comparison of DNA yields from rat feces using the ZR Fecal DNA and kits from suppliers M and Q. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate. Comparative Overview Fecal Sample Only Fecal Sample + E. coli (puc19) M 1 10-1 10-2 10-3 10-4 10-5 10-6 1 10-1 10-2 10-3 10-4 10-5 10-6 The results of PCR of DNA isolated from human feces with and without the addition of E. coli containing puc19 plasmid DNA (indicated at the top of the image) using primers specific for the puc19 sequence. In each case, amplicons were analyzed in a 1.5% (w/v) agarose/ethidium bromide gel using a UV imager. Numbers above each lane of the gel images are the volumetric equivalent (in µl) of eluted DNA used for PCR. Arrows mark the relative migration of amplicons in the gels, and M is a 100 bp DNA ladder (NEB). ZR Fecal DNA Supplier M Supplier Q Processing Time 10-15 minutes 40- minutes 40- minutes DNA Yield Fair Low DNA Integrity Good Fair Poor Fidelity of PCR Fair Use of Proteinases Yes Use of Organic Denaturants ZR Tissue & Insect DNA Kits light Simple and efficient isolation of DNA from insects, including mosquitoes, bees, lice, ticks, and D. melanogaster. Also compatible with tough-to-lyse tissues from other organisms. P Soft & Solid Tissues (Food) P Tough-to-Lyse Tissues 1 kb D. melanogaster n = 10 D. melanogaster n = 20 D. melanogaster larvae n = 10 D. melanogaster larvae n = 20 Darkling Beetle larva Cricket 1 kb Mouse Kidney Mouse Liver Mouse Tailsnip P Tough-to-Lyse Organisms P Insects/Arthropods Cat # Desc. s D6015 Micro D6016 D6115 25 D6017 96-Well 2 x 96 DNA yields from various insect and mouse samples using the ZR Insect & Tissue DNA. Various amounts of sample were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The 1 kb DNA size marker is from Zymo Research. 6 www.zymoresearch.com

ZR Plant DNA Kits Simple methods for the isolation of DNA from tough-to-lyse plant and seed samples in minutes. Fast-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors (tannins) from the DNA product. P Plant Material P Seeds P Fruit Cat # Desc. s D6022 Micro D6020 D6120 25 D6021 96-Well 2 x 96 Plant M 1 2 Seed M 3 4 5 6 Comparison of DNA yields from various plant and seed samples using the ZR Plant/Seed DNA. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 1 kb DNA size marker (Zymo Research). Arabidopsis thaliana (1), juniper (2), corn kernel (3, 4), sunflower seed (5, 6). Without Zymo-Spin IV-HRC With Zymo-Spin IV-HRC M 0 0.1 0.01 0.001 0 0.1 0.01 0.001 PCR of diluted DNA (0 to 0.001) isolated with the ZR Plant/Seed DNA Kit from Arabidopsis thaliana leaf samples demonstrates the effectiveness of the Zymo-Spin IV-HRC Column at removing PCR inhibitors from the DNA. The arrow shows the relative migration of a ~700 bp amplicon from Chromosome 1 in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 100 bp DNA size marker (Zymo Research). BashingBead Lysis for Environmental RNA Purification ((( ))) Pulverize Sample with ZR BashingBead Lysis Tube The BashingBead RNA purification kits from Zymo Research are designed for quick recovery of RT-ready total RNA from tough-to-lyse environmental samples. RNA can be isolated from a broad range of samples including plants, seeds, insects and microorganisms in soil, sludge, sediment, or fecal samples. Kits are available in Micro (5 μg/ prep) and (25 μg/prep) spin column formats (see illustrations). For processing, samples are simply transferred to the provided ZR BashingBead Lysis Tubes and then rapidly and efficiently processed by bead beating in specially formulated lysis buffers. Bead beating can be performed in any bead mill, pulverizer, or vortex that can accommodate standard 2.0 ml tubes. Following lysis, RNA is purified using innovative Fast-Spin column technology. Special filtration technologies are implemented for plant, fecal, and soil samples to remove polyphenolic inhibitors that can inhibit reverse transcriptase (RT). The isolation of inhibitor-free RNA typically takes about 15 minutes. Tissue & Insect RNA or Fungal/Bacterial RNA Inhibitor-free RNA Eliminate DNA with Zymo-Spin IIIC Bind, Wash, Elute RNA with Zymo-Spin IC or IIC Inhibitor-free RNA Plant RNA Eliminate DNA with Zymo-Spin IIIC Bind, Wash, Elute RNA with Zymo-Spin IIC Polyphenolic Removal with Zymo-Spin IV-HRC Inhibitor-free RNA Soil/Fecal RNA Eliminate DNA with Zymo-Spin IIIC Bind, Prewash, Elute RNA with Zymo-Spin IIIC Polyphenolic Removal with Zymo-Spin IV-HRC Clean & Concentrate RNA with Zymo-Spin IC or IIC www.zymoresearch.com 7

Application Guide: Environmental RNA Purification = Spin Column ZR Soil/Fecal RNA ZR Fungal/Bacterial RNA ZR Tissue & Insect RNA ZR Plant RNA Micro Micro Micro Specifications BashingBead Lysis P P P P P Format Binding Capacity 5 µg/prep. 5 µg/prep. 25 µg/prep. 5 µg/prep. 25 µg/prep. Elution Volume 6 µl 6 µl 25 µl 6 µl 25 µl Removal of RT Inhibitors P P P P P Removal of Polyphenolic RT Inhibitors P P Processing Time 20 min. 15 min. 15 min. 15 min. 15 min. Application Environmental Sources Soil P Sediment P Sludge P Feces P Microorganisms Bacteria P P P Gram (+) P P P Gram (-) P P P Fungi P P P Unicellular (Yeast) P P P Filamentous P P P Algae P P P Unicellular P P P Filamentous P P P Protists P P P Solid Tissue and Tough-to-Lyse Samples Soft Tissues some some some P Solid Tissues P Tough-to-Lyse Tissues P Tough-to-Lyse Organisms P Insects/Arthropods P Plant Material P Seeds P Fruit P Food P P P

ZR Soil/Fecal RNA Kits Simple and efficient method for the isolation of inhibitor-free RNA from soil and fecal samples. Ultra-high density BashingBeads can be used with any bead mill, disrupter, or vortex. M RT-PCR +HRC -HRC Control P P P P Soil Sediment Sludge Feces Cat # Desc. s R2040 Micro PCR amplification of a eukaryotic transcript post-rt: Total RNA isolated from sludge with or without inclusion of the Zymo-Spin IV-HRC spin filter during the ZR Soil/Fecal RNA Micro protocol. M is a ZR 1 kb DNA Marker (Zymo Research). ZR Fungal/Bacterial RNA Kits Quick (15 minute) isolation of total RNA from tough-to-lyse bacteria, yeast, and fungi. Omits the use of organic denaturants and proteases. P Gram ( + ) Bacteria P Gram ( - ) Bacteria P Yeast P Filamentous Fungi 9.0-6.0-3.0-2.0-1.0 - RNA Ladder Zymo Research Supplier Q DNA Ladder - 10.0-3.0-1.0-0.5 P Unicellular Algae P Filamentous Algae P Protists P Soft Tissues (limited) P Food Cat # Desc. s R2010 Micro R2014 0.5 - kb DNase I * DNase I Total RNA was isolated from equal amounts of pelleted E. coli cells containing plasmid DNA (pgem ) using the ZR Fungal/Bacterial RNA Micro or the kit from Supplier Q. The samples were resolved in a 2% (w/v) agarose gel. RNA Millenium Markers (Ambion) and ZR 1 kb DNA Marker (Zymo Research) were used. * = genomic (> 10 kb) and plasmid (> 3 kb) DNA contamination; DNase I = samples treated with DNase I. kb www.zymoresearch.com 9

ZR Tissue Insect RNA Kits Quick (15 minute) isolation of RNA from insects and tough-to-lyse tissues. Omits the use of organic denaturants and proteases. M 1 2 9.0-6.0 5.0 4.0 - P Soft Tissues P Solid Tissues 3.0 2.5 - P Tough-to-Lyse Tissues P Tough-to-Lyse Organisms 2.0 - P Insects/Arthropods 1.5 - P Food 1.0 - Cat # Desc. s R2030 Micro kb Analysis of ZR Tissue & Insect RNA Micro. Isolation of total RNA from n=2 Drosophila sp. individuals was performed in duplicate (lanes 1 and 2). Samples were processed (2 x 30 sec at 6 m/s) using a Fast -24 Instrument (MP Biomedicals) and resolved alongside (lane M) RNA Millenium Markers (Ambion) in a 1% (w/v) non-denaturing agarose gel. ZR Plant RNA Kits Quick (15 minute) isolation of inhibitor-free total RNA from a variety of plant tissues. Fast-spin column technology coupled with filtration removes polyphenolic RT inhibitors from the RNA product Sample 1 Sample 2 P Plant Material P Seeds P Fruit Cat # Desc. s R2024 Isolation of total RNA from 10 mg of a fresh leaf material (Nicotiana sp.) using the ZR Plant RNA. Leaves were minced then processed using a Fast -24 instrument (MP Biomedicals). Samples 1 and 2 were loaded in 2x and 1x volume aliquots, respectively, and resolved in a 1% (w/v) nondenaturing agarose gel. RNA Millenium Markers (Ambion) were used as size standards. 10 www.zymoresearch.com

OneStep PCR Inhibitor Removal Kits Removes PCR inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA. Fast, one-step procedure for cleaning impure samples prior to PCR, sequencing, reverse transcription (RT), etc. P P Polyphenolic PCR Inhibitor Removal from DNA Polyphenolic RT Inhibitor Removal from RNA Cat # Desc. s D6030 Spin Column D6035 96-Well 2 x 96 OneStep PCR Inhibitor Removal Kit Procedure Spin Column Format 96-Well Format Add Sample 300 bp - 200 bp - 100 bp - Ladder Controls DNA Sample Containing Humic Acid n-treated Treated Product DNA is efficiently amplified by PCR following humic acid removal with the OneStep PCR Inhibitor Removal Kit. The figure shows amplification of a 200 bp product from DNA containing humic acid that was treated with the kit. Alternatively, PCR amplification was completely inhibited in the case of the non-treated sample. In each case, equal amounts of DNA were used for each PCR and equivalent amounts of the reaction were then analyzed in a 2.0% (w/v) agarose/tae/etbr gel. The ladder is a 100 bp DNA marker (Zymo Research). Hot start PCR was performed using ZymoTaq PreMix (Zymo Research). M +HRC RT-PCR -HRC Control Inhibitor-Free DNA (or RNA) PCR amplification of an eukaryotic transcript (post-rt): Total RNA isolated from sludge with or without inclusion of the Zymo-Spin IV-HRC Spin Filter. M is a 1 kb DNA Marker (Zymo Research). ZR BashingBead Lysis Tubes & Racks ZR BashingBead Lysis Tubes (0.5 mm or 2 mm) Each impact resistant 2.0 ml tube contains 0.7 ml (dry volume) of ZR BashingBead lysis matrix, available in 0.5 or 2 mm. These state-of-the-art, ultra-high density beads are fracture resistant and chemically inert and ideal for disrupting tough-to-lyse bacteria, yeast, fungi, and algae. Cat # Desc. Qty. S6002- Tube (0.5 mm) S6003- Tube (2.0 mm) S6002-96-1 S6002-96-2 Rack (0.5 mm) Rack (2.0 mm) 1 1 ZR-96 BashingBead Lysis Rack (0.5 mm or 2 mm) Each impact resistant 1.1 ml tube contains 0.5 ml dry volume of ZR BashingBead lysis matrix, available in 0.5 or 2 mm. Tubes are in a 96-well rack with caps and a cover for high throughput processing. Perfect for microbes and fungi in soil, feces, sludge, etc. www.zymoresearch.com 11

Environmental DNA D6003 ZR Soil Microbe DNA Micro preps. D6001 ZR Soil Microbe DNA preps. D6101 ZR Soil Microbe DNA 25 preps. D6002 ZR-96 Soil Microbe DNA Kit 2 x 96 preps. D6007 ZR Fungal/Bacterial DNA Micro preps. D6005 ZR Fungal/Bacterial DNA preps. D6105 ZR Fungal/Bacterial DNA 25 preps. D6006 ZR-96 Fungal/Bacterial DNA Kit 2 x 96 preps. D6012 ZR Fecal DNA Micro preps. D6010 ZR Fecal DNA preps. D6110 ZR Fecal DNA 25 preps. D6011 ZR-96 Fecal DNA Kit 2 x 96 preps. D6015 ZR Tissue & Insect DNA Micro preps. D6016 ZR Tissue & Insect DNA preps. D6115 ZR Tissue & Insect DNA 25 preps. D6017 ZR-96 Tissue & Insect DNA Kit 2 x 96 preps. D6022 ZR Plant/Seed DNA Micro preps. D6020 ZR Plant/Seed DNA preps. D6120 ZR Plant/Seed DNA 25 preps. D6021 ZR-96 Plant/Seed DNA Kit 2 x 96 preps. Environmental RNA R2040 ZR Soil/Fecal RNA Micro preps. R2010 ZR Fungal/Bacterial RNA Micro preps. R2014 ZR Fungal/Bacterial RNA preps. R2030 ZR Tissue & Insect RNA Micro preps. R2024 ZR Plant RNA preps. Cell Disrupters & Accessories S6001-2-120 120V 1 unit Disruptor Genie S6001-2-230 European Plug 1 unit Bullet Blender S6007-2 BBX24B Bullet Blender Blue (24 x 1.5-2.0 ml tubes) with cooling fan 1 unit S6007-1 BBX24 Bullet Blender (24 x 1.5-2.0 ml tubes) 1 unit S6007-3 BBXB Bullet Blender Blue (9 x ml tubes) with cooling fan 1 unit Fast -24 S6005 Fast -24 1 unit Fast -24 Acessories S6005-1 Hi Attachment (48 x 2 ml tubes) 1 unit S6005-2 Cool Attachment (24 x 2 ml tubes) 1 unit S6005-3 Teen Attachment (12 x 15 ml tubes) 1 unit S6005-4 Big Attachment (2 x ml tubes) 1 unit S6005-5 Fast European AC Cord 1 unit 2010 Geno/Grinder S6006 2010 Geno/Grinder 1 unit 2010 Geno/Grinder Accessories S6006-1 2 ml Tube Holder/Cryo Block Assembly (48 x 2.0 ml tubes/block) 2 blocks S6006-2 15 ml Tube Holder/Cryo Block Assembly (15 x 15 ml tubes/block) 2 blocks S6006-3 ml Tube Holder/Cryo Block Assembly (6 x ml tubes/block) 2 blocks S6006-10 Large Capacity Clamp Assembly 1 unit also available D6030 OneStep PCR Inhibitor Removal Kit preps. D6035 OneStep-96 PCR Inhibitor Removal Kit 2 x 96 preps. S6002- ZR BashingBead Lysis Tubes (0.5 mm) tubes S6003- S6002-96-1 ZR BashingBead Lysis Tubes (2.0 mm) ZR-96 BashingBead Lysis Rack (0.5 mm) tubes 1 rack For a complete product listing, please visit www.zymoresearch.com S6002-96-2 ZR-96 BashingBead Lysis Rack (2.0 mm) 1 rack Find us on Facebook & follow us on Twitter for the latest product releases, special offers, and more!