Ergonomic Pipetting for Problem Liquids!

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HIGHEST RECISION WITH ROBLEM LIQUIDS USER-FRIENDLY NO RISK OF CONTAMINATION O S I T I V E D I S L AC E M E N T I E T T E Ergonomic ipetting for roblem Liquids!

ADVANCED ERGONOMIC DESIGN TO INCREASE IETTE ERFORMANCE The MICROMAN E delivers the highest performance to scientists who need to pipette problem liquids thanks to: EASE OF USE: atented QuickSnap feature automatically fixes the disposable Capillary iston (C), thus eliminating the need to fit the C to the pipette before each use and minimizing possible errors due to additional pipetting moves Volume control button prevents accidental volume changes IMROVED ERGONOMIC DESIGN: Large action button Comfortable finger hook Clearly visible volumeter Volume control button atented QuickSnap: easy C fitting

INTRODUCING THE MICROMAN E, A NOVEL IETTE LINE FOR ROBLEM LIQUIDS The MICROMAN E is created specifically for scientists who work with viscous, volatile, corrosive, hot or cold problem liquids. Delivering the highest ease-of-use and control through an improved ergonomic design, a patented QuickSnap feature and a volume control button, the MICROMAN E helps you counter your common pipette problems that may affect accuracy and precision when working with problem liquids. MICROMAN E is available in 6 models covering a volume range from 1 µl to 1000 µl for many applications. MAIN ALICATIONS MAIN ROTOCOLS ROBLEM LIQUIDS TYES EXAMLES Molecular biology CR/qCR, cloning Viscous, cold and contaminating DNA fragments, buffers, enzyme solution, plasmids Chemistry, petrochemistry HLC, LC-MS Volatile, hazardous Alcohol, solvents, glue, oil, organic solvents oint of care diagnostic Quantitative immunoassays Viscous, cold and hazardous Serum, whole blood, plasma Forensic Cosmetic and pharmaceutical formulation Environment, quality control Food quality control Liquids search in post mortem blood, biological control Bacteriology control, cream transfers, takings and dilution of solvents and high viscosity finished product Microbiological control and other biological tests Microbiological control and other biological tests Volatile, viscous and contaminant liquids Viscous, volatile Corrosive and volatile High viscosity, hot DNA fragments, body fluids, solvents Creams, shampoo, drugs, gel esticides, waste waters Syrup, milk, juices

THE HIGHEST RECISION WITH VISCOUS, VOLATILE, HOT OR COLD LIQUIDS As a positive displacement pipette, the MICROMAN E with its Cs solves common pipetting problems by isolating the aspirated sample from the inside part of the pipette, eliminating the air space between the piston and sample, to offer the highest precision for pipetting problem liquids. 1. EXERIENCE COMLETE AND RAID IETTING OF VISCOUS AND DENSE SAMLES. roblem avoided: With air displacement pipettes, viscous and dense liquids are difficult to aspirate and can also stick to the tip when dispensing. 2. NO LEAKS WHEN IETTING VOLATILE HIGH VAOR RESSURE LIQUIDS. roblem avoided: When an organic solvent is used with an air-displacement pipette, leaks may occur due to the difference between the vapor pressure of the solvent and the pressure of the air cushion between the tip and the sample. IETTING 100 µl OF VISCOUS OR VOLATILE LIQUIDS 4.00 % SYSTEMATIC ERROR 2.00 0-2.00-4.00-6.00-8.00-10.00 MICROMAN E Glycerol, 100% Acetone Regular ipette Average of 10 measurements, no pre-wetting 3. DELIVER THE RIGHT VOLUME USING ANY SAMLE TEMERATURE. roblem avoided: Cold liquids tend to be delivered in excess quantity while a warm liquid tends to be under-delivered. IETTING 100 µl OF HOT LIQUIDS 4.00 % SYSTEMATIC ERROR 2.00 0-2.00-4.00-6.00-8.00-10.00 MICROMAN E coffee 75-85 C Regular ipette hot tap water 75-85 C Average of 10 measurements, no pre-wetting

NO RISK OF CONTAMINATION MICROMAN E and Cs are the strongest barrier against residual sample carryover, aerosol contamination, and crosscontamination when pipetting liquids such as DNA fragments, CR templates, enzyme solutions and buffers. REGULAR AIR DISLACEMENT IETTE Unprotected air space: Vapor contamination MICROMAN E OSITIVE DISLACEMENT IETTE No air space Aerosol contamination With the MICROMAN E, you protect your pipette, your sample and yourself in three ways: 1. User protection: With a built-in ejector, there is no contact between the sample and the contaminated disposable capillary with the user s hand. This is especially important when dealing with infectious solutions and radiolabeled components. 2. Sample protection: Within molecular biology and forensic sciences, any trace of a previous sample can interfere with CR or microbiological techniques and lead to erroneous results. Disposable capillaries and pistons provide absolute protection against cross-contamination. 3. ipette protection: The pipette s shaft is never in contact with corrosive vapors or liquids, such as acids, bases, solvents and radiolabeled components.

TRUSTED RESULTS GUARANTEED MICROMAN E s highest level of accuracy and precision is achieved by skillful engineering based on over 30 years of experience with positive displacement pipettes. Today MICROMAN E has become the international gold standard in pipetting for viscous, volatile, hazardous, contaminating, hot or cold liquids. MAXIMUM ERMISSIBLE ERRORS MODEL VOLUME GILSON ISO 8655 (Reference) (μl) Systematic error (μl) Random error (μl) Systematic error (μl) Random error (μl) M10E (FD10001) 1 5 10 ± 0.09 ± 0.10 ± 0.15 0.03 0.03 0.06 ± 0.2 ± 0.2 ± 0.2 0.1 0.1 0.1 M25E (FD10002) M50E (FD10003) M100E (FD10004) M250E (FD10005) M1000E (FD10006) 3 10 25 20 50 10 50 100 50 100 250 100 500 1000 ± 0.25 ± 0.27 ± 0.30 ± 0.34 0 ± 0.50 5 ± 1.00 ± 1.50 ± 1.70 ± 2.50 ± 3.0 ± 5.0 ± 8.0 0.08 0.08 0.10 0.20 0 0.20 0 0.40 0 0 0.50 1.6 2.5 ± 1.5 ± 1.5 ± 1.5 ± 6 ± 6 ± 6 ± 12 ± 12 ± 12 0.6 0.6 0.6 2.0 2.0 2.0 MICROMAN Cs Large volume range from 1 μl to 1000 μl with six different models. No need for calibration when you change the C. Disposable C, capillaries made of pure polypropylene. Free of dyes, no risk or contamination from the C s components. Slim C fits in even the narrowest and deepest tubes. MICROMAN MODEL M10E M25E M50E M100E M250E M1000E RELATED C C10 C10 C10ST* C10ST* C25 C25 C25ST* C50 C50 C50ST* C100 C100 C100ST* C100ST* C250 C250 C250ST* C1000 C1000ST* VOLUME RANGE (μl) 1-10 3-25 20-50 10-100 50-250 100-1000 ORDERING REFERENCE F148412 F148312 F148413 F148313 F148012 F148112 F148712 F148013 F148113 F148713 F148414 F148314 F148415 F148315 F148014 F148114 F148714 F148560 F148180 QUANTITY ER BOX 200 576 200 576 200 576 182 182 AVAILABLE IN TIACK - - - *ST=Sterilized www.gilson.com I sales@gilson.com I service@gilson.com I training@gilson.com Gilson, Inc. World Headquarters I 3000 armenter Street I.O. Box 620027 Middleton, WI 53562-0027 USA Tel: (1) 800-445-7661 or (1) 608-836-1551 I Fax: (1) 608-831-4451 Gilson S.A.S. I 19, avenue des Entrepreneurs B 145, F-95400 Villiers-le-Bel, FRANCE Tel: +33(0)1 34 29 50 00 I Fax: +33 (0)1 34 29 50 20 LT800548 10/2014 Gilson SAS. All rights reserved. All trademarks are the property of Gilson Inc. and its subsidiaries.