Product Datasheet TGN38 Antibody NBP1-03495 Unit Size: 0.5 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 5 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nbp1-03495 Updated 1/30/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/nbp1-03495
NBP1-03495 TGN38 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Reconstitution Instructions Isotype Purity Buffer 0.5 mg Please see the vial label for concentration. If unlisted please contact technical services. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Polyclonal No Preservative Reconstitute with 0.5 ml of sterilized water to desired concentration. Centrifuge to remove any insoluble material. Note that the 0.1mg size ships already reconstituted. IgG IgG purified PBS Page 1 of 4 v.20.1 Updated 1/30/2018 Product Description Description Host PLEASE NOTE: 0.1mg sample size is provided in reconstituted format and the 0.5mg size is provided lyophilized. (Please see reconstitution instuctions). Rabbit Gene ID 10618 Gene Symbol Species Marker Specificity/Sensitivity Immunogen TGOLN2 Human, Mouse, Rat TGN Marker TGN38 (or TGN46, TGN48, TGN51 in human). A synthetic peptide from the cytoplasmic part of mouse TGN38 conjugated to an immunogenic carrier protein was used as the immunogen. Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry Western Blot 10-50 ug/ml, Immunohistochemistry 10-50 ug/ml, Immunocytochemistry/Immunofluorescence 1:50-1:250 The 0.1 mg size ships reconstituted and the 0.5 mg size ships lyophilized.
Images 03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides. Page 2 of 4 v.20.1 Updated 1/30/2018 03495] - Human Melanoma cell line C 32. 03495] - Human Melanoma cell line C 32 03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
Publications Page 3 of 4 v.20.1 Updated 1/30/2018 Ansari IH, Longacre MJ, Stoker SW et al. Characterization of Acyl-CoA synthetase isoforms in pancreatic beta cells: Gene silencing shows participation of ACSL3 and ACSL4 in insulin secretion. Arch. Biochem. Biophys. 2017 Feb 11 [PMID: 28193492] (WB, Human) Sugahara D Kobayashi Y Akimoto Y, Kawakami H. Mouse intestinal niche cells express a distinct a1,2-fucosylated glycan recognized by a lectin from Burkholderia cenocepacia. Glycobiology. Nov 22 2016 12:00AM [PMID: 27979999] (Mouse) Fernandez-Fernandez MR, Ruiz-Garcia D, Martin-Solana E et al. 3D electron tomography of brain tissue unveils distinct Golgi structures that sequester cytoplasmic contents in neurons. J. Cell. Sci. Aug 5 2016 12:00AM [PMID: 27505890] (ICC/IF, Mouse) Ansari IH, Longacre MJ, Paulusma CC et al. Characterization of P4 ATPase Phospholipid Translocases (Flippases) in Human and Rat Pancreatic Beta Cells: Their Gene Silencing Inhibits Insulin Secretion. J. Biol. Chem. 2015 Aug 03 [PMID: 26240149] (WB, Human) Ting CH, Wen HL, Liu HC et al. The Spinal Muscular Atrophy Disease Protein SMN Is Linked to the Golgi Network PLoS One 2012 [PMID: 23284781] (ICC/IF, WB, Mouse)
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