DNA Structure and Function DNA Structure DNA Replication Ch 4: Nucleic Acids and the Origin of LIfe Ch 13: DNA and its role in heredity
Discussion Summary: Week 12 Stirring the Simmering Designer Baby Pot Team Discussion: Questions Take home message Group discussion Take home messages - one from each group Questions- one from each group
Nucleic Acid Structure ff Purine / Pyrimidine Base pair Ribose Phosphate Nucleotide Double-helix Access Excellence http://www.accessexcellence.org/rc/vl/gg/dna_molecule.php Michael Blaber http://www.mikeblaber.org/oldwine/bch5425/lect02/lect02.htm
Nucleic Acid Structure ff Access Excellence http://www.accessexcellence.org/rc/vl/gg/dna_molecule.php Michael Blaber http://www.mikeblaber.org/oldwine/bch5425/lect02/lect02.htm DNA Structure Purine / Pyrimidine Base pair
Nucleic Acid Structure Ribose Deoxyribose Michael Blaber h8p://www.mikeblaber.org/oldwine/bch5425/lect02/lect02.htm DNA Structure
Nucleic Acid Structure Phosphate Nucleotide Michael Blaber http://www.mikeblaber.org/oldwine/bch5425/lect02/lect02.htm DNA Structure
Nucleic Acid Structure Nucleotide polymer Michael Blaber http://www.mikeblaber.org/oldwine/bch5425/lect02/lect02.htm DNA Structure
DNA Structure B form of DNA Anti-parallel
Biochemistry Chargaff Modeling Crick and Watson Structural Biology Rosalind Franklin DNA Structure and Function Multidisciplinary Endeavor
Central Dogma DNA à RNA à Protein DNA Function ERA Net http://www.ict-science-tosociety.org/pathogenomics/ genome.aspx
DNA Function Gene Structure Promoter 5 UTR Coding region 3 UTR Terminator University of Arizona http://nitro.biosci.arizona.edu/courses/eeb600a-2003/lectures/lecture24/lecture24.html
DNA and Heredity! Classic experiments
DNA as Genetic Material Classic Experiments Griffith s Transformation Streptococcus pneumoniae How did the mouse die when the only living strain was not virulent? DNA and heredity U of Cincinnati Claremont: Dr. Frankhauser http://biology.clc.uc.edu/fankhauser/labs/genetics/lectures/lecture_htm/01_genetics_history.htm
DNA as Genetic Material Classic Experiments Avery, MacLeod, McCarty Which fraction of the virulent cell can transform the non virulent strain? Vision Learning.com http://www.visionlearning.com/library/module_viewer.php?mid=149 DNA and heredity
DNA as Genetic Material Classic Experiments Hershey and Chase E. coli Which molecule from a phage moves to the bacterial cell? Access Excellence http://www.accessexcellence.org/rc/vl/gg/hershey.php DNA and heredity
Investigating Life : Terminology Transformation Permanent DNA change to Bacterial and non-animal Eukaryotic cells Transfection Impermanent DNA change Sometimes means any added DNA in animals Transduction Chapter 13 Using a virus vector DNA and heredity h8p://www.biotechnologyforums.com/ thread-1775.html
DNA Replication Steps Break H-bond New strand RNA primer Players Helicase DNA polymerase Template DNA Nucleotides (dntp s) Result 2 DNA double-helices, identical copies of one Extra details Methylation of new DNA DNA repair Mutations 5 to 3 (3 to 5 possible, but does not happen)
DNA Replication Overview Circular DNA Important proteins Replication fork Leading/lagging strands Termination What when how.com http://what-when-how.com/molecular-biology/dna-replication-molecular-biology/ Contexto Info http://www.contexo.info/dna_basics/dna%20replication.htm
Semiconservative Replication Experiment Meselson-Stahl Grow cells in heavy nitrogen source ( 15 N) Density-gradient centrifugation of DNA. Let cells replicate in normal nitrogen source ( 15 N) Density-gradient centrifugation of DNA Repeat for another generation Steven Carr http://www.mun.ca/biology/scarr/igen3_03-02.html DNA Replication
DNA Replication Overview Circular DNA Important proteins Replication fork Leading/lagging strands Termination Supercoling and replication: Stone et al http://www.pnas.org/content/100/15/8654/f1.expansion.html DNA Replication
DNA Replication Overview Circular DNA Important proteins Replication fork Leading/lagging strands Termination Wikipedia http://en.wikipedia.org/wiki/replication_fork Dolan DNA learning Center http://www.dnalc.org/resources/3d/03-mechanism-of-replication-basic.html http://youtu.be/gw3qzf9clia
DNA Replication Overview Circular DNA Important proteins Replication fork Leading/lagging strands Termination: DNA gyrase splits Nobel Overviews: http://www.nobelprize.org/educational/ medicine/dna/a/replication/ replication_ani.html http://www.nobel.se/medicine/educational/ dna/a/replication/lagging_ani.html Wikipedia http://en.wikipedia.org/wiki/replication_fork
Some great DNA animations https://youtu.be/dkubyirin84 Chromosomes are always there just not easy to see http://sites.fas.harvard.edu/~biotext/animations/ replication1.swf Harvard is always accurate =) https://www.dnalc.org/resources/3d/04-mechanism-ofreplication-advanced.html So is Cold Spring Harbor! http://www.hhmi.org/biointeractive/chemical-structuredna http://www.hhmi.org/biointeractive/dna-replicationschematic Don t forget Howard Hughes Medical Institute (HHMI)
STRETCH BREAK!
STRETCH BREAK!
This week s lab: Lab 5a Intro Punnett squares Meiosis generates a diversity haploids via crossing-over, independent assortment Fertilization generates diversity because it is random Punnett squares help us figure out all possible combinations Real Data Track real phenotypes in progeny from known crosses Compare with predictions Molecular genotyping Genotypes can be inferred through phenotypes and pedigrees Genotypes can be determined directly through molecular means (PCR, restriction digests, sequencing)
Next week s lab: Lab 5b week 1 intro Culture Pseudomonas syringae (2 strains) for innoculations Prepare dilutions of bacterial solutions for controlled experiment Innoculate A. thaliana with pathogen Predict phenotypes based on genotypes and classical genetic analysis from lab 5a