Tomatoes from Tomatoes Using the Effluent of Anaerobically Digested Tomatoes to Fertilize Tomato Plants By Jacquelyn Neal BioEnergy Summer School 2011
Outline Introduction to anaerobic digestion Problem Objectives Method Results Areas for further study Implications to overall sustainability
Anaerobic Digestion Microbes break down biodegradable materials in a non-oxygen environment Forms methane and other gases which can be used for energy Manages waste Effluent, or digestate, is another product of this process. It is often thought to be a possible biofertilizer.
Energy Waste (culls) Biogas Biofertilizer Biodigestion Soil
Problem Can anaerobically digested tomato culls be used to grow tomatoes?
Objectives To determine if anaerobically digested tomato effluent can be used as an effective substitute fertilizer for tomatoes (compared with NPK and organic fertilizers) To determine an effective rate of application for effluent from anaerobically digested tomatoes
Method 400 ml BMP bottles (15) were filled with effluent from an anaerobic digester at the BEST Lab and filled with 2 g COD/L of tomato culls (14.41 grams tomato culls) The 16 th bottle was filled with only effluent and served as a blank for testing
Methods (cont) Three BMP bottles were tested at random for ammonia content. The average concentration of the three was 533 ppm. The amount of effluent needed for each treatment was calculated and then diluted to 250 ppm with tap water The plants were fertilized, and each plant was watered 250 ml every day (except when it rained)
Methods (cont) A soil substitute was created using peat moss and perlite. For every kg of peat moss, 200 g of perlite was used. Pepper plants were substituted for the tomato plants in this experiment as pepper plants are better adapted to Florida s summer climate. The pepper plants were transplanted to 1 gallon pots and fertilizer was applied six days later
Methods (cont) Synthetic NPK fertilizer: Miracle-Gro Organic fertilizer: Fish emulsion Anaerobically digested tomatoes: Three rates of application: Low: 10 kg N/ha Medium: 20 kg N/ha High: 30 kg N/ha Five Treatments The NPK and organic fertilizer were each applied at 20 kg N/ha
Methods (cont) Each plant was measured every three days and the leaves were counted. Ultimately, the leaf data was collected but not used in the analysis. A seed germination test was conducted to determine the viability of tomato seeds post-anaerobic digestion
Treatment Results (cont) Average Cumulative Height Change (cm) Standard Deviation Control 2.00 1.32 MiracleGro 4.00 3.04 Fish Emulsion 4.17 2.93 ADE Rate 1 3.67 0.76 ADE Rate 2 6.50 4.00 ADE Rate 3 5.33 0.29
Results (cont) Dish 1 Dish 2 Total % Germinated Fresh Seeds 8 9 56.67 Frozen Seeds 0 0 0 Anaerobically Digested Seeds 0 0 0
Implications of the Seed Germination Test Only the fresh seeds germinated. The post-anaerobic digestion seeds did not germinate, so if effluent of anaerobically digested tomatoes were used as a fertilizer, unwanted plants would not sprout. Tomato processors have previously been unable to flush tomato waste because of seed germination If processors were unable to use the effluent as fertilizer, they might have the option of flushing their effluent, as the seeds would not germinate
Areas for Further Study Nutrient testing: Test effluent for phosphorus and potassium, as it may needs supplements Measuring plant growth at the end of experiment by measuring total biomass Use effluent from a digester that continuously digests tomato culls Larger-scale growth experiment more plants per treatment longer time frame with multiple treatment applications
Implications for Sustainability There is potential for the effluent of anaerobically digested tomato culls to be used as a fertilizer for tomato plants Effluent quality is dependent upon the feedstock, there could be high variability between batches of effluent fertilizer, so nutrient testing must occur before use. Tomato culls as a feedstock could yield more continuous nutrient content. If effluent were developed as a fertilizer, the tomato industry could become a closed-loop system
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